• Journal of the Optical Society of Korea
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• 제13권3호
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• pp.335-340
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• 2009

Ultrashort pulse laser drilling of polycarbonate track-etched membrane (pTEM) material was used to fabricate a mouse embryo cell trapping device. Holes with a diameter of $2{\mu}m$ to $5{\mu}m$ were fabricated on a $10{\mu}m$ thick membrane using a femtosecond laser with a 150 fs pulse width and 775 nm wavelength and multiple-pulse irradiation. In cell trapping tests, the overall cell occupancy of the machined holes in the fabricated pTEM was found to be more than 80%. The results of a single pulse and multiple pulse irradiation were compared in terms of the surface quality. It was generally found that a single pulse with high energy was less desirable than irradiation with multiple pulses of lower energy.

• Corrosion Science and Technology
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• 제15권2호
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• pp.43-53
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• 2016

In this study, electrochemical impedance spectroscopy (EIS) was used to examine the changes in the electrochemical properties of biodegradable pure magnesium implanted into Sprague-Dawley rats for three days. The in vivo test results were compared with those of the in vitro tests carried out in Hank's, dilute saline and simulated body fluid (SBF) solutions. The in vitro corrosion rates were 20~1700 fold higher, as compared to the in vivo corrosion rates. This discrepancy is caused by biomolecule adsorption on the surface, which prevents the transport of water into the magnesium surface on in vivo testing. Among the in vitro experimental conditions, the corrosion rate in SBF solution had the least difference from the in vivo implanted specimen.

• Journal of Microbiology and Biotechnology
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• 제21권11호
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• pp.1143-1146
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• 2011

Metabolic engineering of plant-specific phenylpropanoid biosynthesis has attracted an increasing amount of attention recently, owing to the vast potential of flavonoids as nutraceuticals and pharmaceuticals. Recently, we have developed a recombinant Streptomyces venezuelae as a heterologous host for the production of flavonoids. In this study, we successfully improved flavonoid production by expressing two sets of genes predicted to be involved in malonate assimilation. The introduction of matB and matC encoding for malonyl-CoA synthetase and the putative dicarboxylate carrier protein, respectively, from Streptomyces coelicolor into the recombinant S. venezuelae strains expressing flavanone and flavone biosynthetic genes resulted in enhanced production of both flavonoids.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.61-61
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• 2002

No Abstract, See Full Text

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.60-60
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• 2002

No Abstract, See Full Text

• 한국정밀공학회지
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• 제33권9호
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• pp.699-706
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• 2016

Assay of biomolecules based on absorption spectroscopy is a sensitive, minute, quantitative, rapid and real-time process. UV-vis spectrometry has inherent advantages for use in analytical applications. To reduce the consumption of scarce samples, a new generation of miniaturized UV-vis spectrometric systems has been developed. This study determined empirically and through simulations the feasibility of assaying biomolecules by means of UV-vis spectrometry. This paper also reports a fully integrated portable micro-detection equipment system that utilizes a micro-optical path for analysis of micro-volume samples.

• Applied Microscopy
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• 제48권4호
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• pp.81-86
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• 2018

The respiratory chain complex forms a supercomplex (SC) in the inner mitochondrial membrane. This complex facilitates the process of electron transfer to produce the proton gradient used to synthesize ATP. Understanding the precise structure of the SC is considered an important challenge. However, it has not yet been reported. The development of a Cryo-electron microscopy (EM) technique provides an effective way to obtain high-resolution micrographs to determine the high-resolution three-dimensional structure of biomolecules. In this brief review, the currently reported Cryo-EM structures of the mammalian respirasome have been described in order to establish a direction for further research in the respiratory system.

• 센서학회지
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• 제31권6호
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• pp.389-396
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• 2022

Quartz crystal microbalance (QCM) based sensors are used for various applications owing to advantages such as excellent accuracy and precision, rapid response, and tiny footprint. Traditional applications of QCM-based sensors include biological sensing and thin-film thickness monitoring. Recently, QCMs have been used as functional material for novel physical and chemical detections, and with improved device design. QCM-based sensors are garnering considerable attention in particulate matter sensing and electric nose application. This review covers the challenges and solutions in physical, chemical, and biological sensing applications. First, various physical sensing applications are introduced. Secondly, the toxic gas and chemical detection studies are outlined, focusing on introducing a coating method for uniform sensing film and sensing materials for a minimal damping effect. Lastly, the biological and medical sensing applications, which use the monomolecularly decorating method for biomolecule recognition and a brief description of the overall measuring system, are also discussed.

• 대한방사성의약품학회지
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• 제6권2호
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• pp.177-181
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• 2020

Dual-modality imaging strategy using near-infrared fluorescence (FLI) and photoacoustic imaging (PAI) demands a suitable probe to enable dual-modular signal production. Herein, we demonstrate a synthetic protocol of small molecular dye for dual-modular FLI and PAI. A condensation reaction between squaric acid and carboxypentyl benzoindolium, and followed by basic hydrolysis to give the benzoindole derived squaraine (BSQ) dye in 49% yield. Next, the carboxylic acid group of BSQ was further functionalized with N-hydroxysuccinimide or azide group for an efficient conjugation with a targeting biomolecule. BSQ showed a maximum fluorescent emission at around 680 nm and the photoacoustic signal reached a maximum intensity at 680-700 nm. Based on these results, we conclude that BSQ analogs will be useful probes for dual-modular (FLI/PAI) imaging studies in animal models.

• 대한수학회논문집
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• 제39권1호
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• pp.223-245
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• 2024

We study RNA foldings and investigate their topology using a combination of knot theory and embedded rigid vertex graphs. Knot theory has been helpful in modeling biomolecules, but classical knots emphasize a biomolecule's entanglement while ignoring their intrachain interactions. We remedy this by using stuck knots and links, which provide a way to emphasize both their entanglement and intrachain interactions. We first give a generating set of the oriented stuck Reidemeister moves for oriented stuck links. We then introduce an algebraic structure to axiomatize the oriented stuck Reidemeister moves. Using this algebraic structure, we define a coloring counting invariant of stuck links and provide explicit computations of the invariant. Lastly, we compute the counting invariant for arc diagrams of RNA foldings through the use of stuck link diagrams.