• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.392-392
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• 2005

• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.921-924
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• 2010

Blocking of IL-6 has been postulated to be an effective therapy in the pathogenesis of several inflammatory diseases. The current study was performed to examine the potential effects of alkamides isolated from P. longum and P. nigrum on IL-6 induced Stat3 activation and identify the structure-activity relationship of these alkamides in human hepatoma cells. Among 10 alkamides isolated from P. longum and P. nigrum, compounds 6, 7 and 9 were identified as strong inhibitors of IL-6 action, which inhibit IL-6 induced Stat3-dependent luciferase activities. These inhibitory activities were positively influenced by the presence of piperidine moiety.

• 한국당과학회:학술대회논문집
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• 2006.11a
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• pp.53-54
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• 2006

• 한국당과학회:학술대회논문집
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• 2006.11a
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• pp.55-55
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• 2006

• Journal of the Korean Society for Precision Engineering
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• v.27 no.6
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• pp.90-97
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• 2010

In order to understand the biomaterial like the blood vessel of artery, there is a need to quantify the biomechanical behavior of the vessel. Using computer-controlled experimental system, the experiment can acquire data such as inner pressure, axial load, diameter and axial gauge length without contacting the specimen. Rubber-liked material which is similar to passive artery was selected as pseudo-biomaterial. Deformations are measured for pressure-diameter curves. The data were collected and stored online to be used in the feedback control of experimental protocols. Finally, the illustrative data obtained from the experimental system were presented and the system shows that strain invariants are controlled to understand the nonlinear elastic behavior of biomaterial which is involved with strain energy function.

• Journal of Microbiology and Biotechnology
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• v.30 no.12
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• pp.1810-1818
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• 2020

Inhibitor K562 (IK) protein was first isolated from the culture medium of K562 cells, a leukemia cell line, and is an inhibitory regulator of interferon-γ-induced major histocompatibility complex class II expression. Recently, exogenous truncated IK (tIK) protein showed potential as a therapeutic agent for inflammation-related diseases. In this study, we designed a novel putative anti-inflammatory peptide derived from tIK protein based on homology modeling of the human interleukin-10 (hIL-10) structure, and investigated whether the peptide exerted inhibitory effects against pro-inflammatory cytokines such as IL-17 and tumor necrosis factor-α (TNF-α). The peptide contains key residues involved in binding hIL-10 to the IL-10 receptor, and exerted strong inhibitory effects on IL-17 (43.8%) and TNF-α (50.7%). In addition, we used circular dichroism spectroscopy to confirm that the peptide is usually present in a random coil configuration in aqueous solution. In terms of toxicity, the peptide was found to be biologically safe. The mechanisms by which the short peptide derived from human tIK protein exerts inhibitory effects against IL-17 and TNF-α should be explored further. We also evaluated the feasibility of using this novel peptide in skincare products.

• Bulletin of the Korean Chemical Society
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• v.31 no.7
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• pp.1931-1936
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• 2010

Discovery and isolation of compounds capable of blocking the interactions between VCAM-1 and VLA-4, a major pair of adhesion molecules contributing to the different steps of leukocyte migration across the endothelium in inflammatory responses, has been a major goal of this lab. Through bioactivity-guided fractionation, five saikosaponins were subsequently isolated from the methanol extracts of the roots of Bupleurum falcatum L. Their structures were elucidated by spectroscopic analysis ($^1H-$, $^{13}C$-NMR and 2D-NMR), as follows, saikosaponins: A (1); D (2); C (3); B3 (4); B4 (5). Compounds 1 and 2 inhibited interaction of sVCAM-1 and VLA-4 of THP-1 cells with respective $IC_{50}$ values of 7.8 and 1.7 ${\mu}M$. The aglycone structure of 2 also showed cell adhesion inhibitory activity with an $IC_{50}$ value of 21.1 ${\mu}M$. With these results, we suspect these two saikosaponins from the Bupleurum falcatum L. roots to be prime candidates for therapeutic strategies towards inflammation.

• Journal of Microbiology and Biotechnology
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• v.22 no.6
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• pp.806-813
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• 2012

Adipocyte differentiation is strongly associated with obesity, which causes metabolic disorders. In this study, we investigated the inhibitory effects of widdrol on 3T3-L1 preadipocyte growth and differentiation. Widdrol decreased lipid droplet accumulation and down-regulated adipogenic transcription factors such as C/$EBP{\alpha}$, C/$EBP{\beta}$, and $PPAR{\gamma}$. Widdrol blocked preadipocyte proliferation and differentiation through the inhibition of mitotic clonal expansion, which was accompanied by the failure of degradation of p21, a cyclin-dependent kinase inhibitor. Cell-cycle analysis clearly indicated that widdrol actively induces cell-cycle arrest at the G1-S phage transition, causing cells to remain in the preadipocyte state. Moreover, widdrol increased p21 expression and inhibited Rb phosphorylation in preadipocyte incubated in a hormone medium. Therefore, these findings clearly suggest that widdrol blocks preadipocyte growth and differentiation through the inhibition of mitotic clonal expansion by p21-and Rb-dependent G1 arrest and can be developed as a potent anti-adipogenic agent for reducing obesity.

• Journal of Veterinary Clinics
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• v.39 no.2
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• pp.59-64
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• 2022

A dog aged two years and seven months and a cat aged seven years were referred owing to fractures of long bones. Preoperative radiographs revealed comminuted bone fractures close to joints. Conventionally, long-bone fractures are treated using intramedullary pins, plate and screw systems, or an external fixator system. In cases of non-reducible fractures, various graft materials have been used in fracture treatments to stimulate bone repair. Here, recombinant human bone morphogenetic protein-2 (rhBMP-2) and a collagen membrane were applied. Four weeks after surgery, fractured bone fragments began to unite and the bone union was observed using radiography four months after surgery. No complications occurred related to grafted materials. We successfully applied rhBMP-2 and collagen membranes in two different species to support the healing process of comminuted fractures, according to the concept of guided bone regeneration.

• Prospectives of Industrial Chemistry
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• v.18 no.4
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• pp.1-9
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• 2015

최근 신체의 손상된 조직 및 세포를 재생하기 위해 약물 전달이 가능한 생체재료에 관한 연구가 활발히 이루어지고 있다. 이러한 생체재료 개발을 위해 생체적합하며 생분해성이 뛰어난 천연고분자가 큰 각광을 받고 있다. 기존의 약물 전달을 위한 고정화 방법은 화학적 가교가 널리 이용되어 왔으나, 이 방법의 여러 단점들이 보고된 바 있다. 이러한 단점을 극복하기 위해 광반응성 천연고분자를 이용한 약물광고정화 방법이 연구되어 왔다. 본 글에서는 광고정화를 위해 합성되는 여러 광반응성 천연고분자의 종류 및 특성과 생체재료로써의 활용 방안을 소개하고자 한다.