Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Widdrol Blocks 3T3-L1 Preadipocytes Growth and Differentiation Due to Inhibition of Mitotic Clonal Expansion

  • Yun, Hee-Jung (Department of Biomaterial Control (BK21 Program), Dong-Eui University Graduate School) ;
  • Kim, Jeong-Hwan (Department of Biomaterial Control (BK21 Program), Dong-Eui University Graduate School) ;
  • Jeong, Hyun-Young (Department of Life Science and Biotechnology, College of Natural Science, Dong-Eui University) ;
  • Ji, Hyang-Hwa (Department of Biomaterial Control (BK21 Program), Dong-Eui University Graduate School) ;
  • Nam, Soo-Wan (Department of Biomaterial Control (BK21 Program), Dong-Eui University Graduate School) ;
  • Lee, Eun-Woo (Department of Biomaterial Control (BK21 Program), Dong-Eui University Graduate School) ;
  • Kim, Byung-Woo (Department of Biomaterial Control (BK21 Program), Dong-Eui University Graduate School) ;
  • Kwon, Hyun-Ju (Department of Biomaterial Control (BK21 Program), Dong-Eui University Graduate School)
  • Received : 2011.10.12
  • Accepted : 2012.02.15
  • Published : 2012.06.28
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Abstract

Adipocyte differentiation is strongly associated with obesity, which causes metabolic disorders. In this study, we investigated the inhibitory effects of widdrol on 3T3-L1 preadipocyte growth and differentiation. Widdrol decreased lipid droplet accumulation and down-regulated adipogenic transcription factors such as C/$EBP{\alpha}$, C/$EBP{\beta}$, and $PPAR{\gamma}$. Widdrol blocked preadipocyte proliferation and differentiation through the inhibition of mitotic clonal expansion, which was accompanied by the failure of degradation of p21, a cyclin-dependent kinase inhibitor. Cell-cycle analysis clearly indicated that widdrol actively induces cell-cycle arrest at the G1-S phage transition, causing cells to remain in the preadipocyte state. Moreover, widdrol increased p21 expression and inhibited Rb phosphorylation in preadipocyte incubated in a hormone medium. Therefore, these findings clearly suggest that widdrol blocks preadipocyte growth and differentiation through the inhibition of mitotic clonal expansion by p21-and Rb-dependent G1 arrest and can be developed as a potent anti-adipogenic agent for reducing obesity.

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References

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