Browse > Article
http://dx.doi.org/10.17555/jvc.2022.39.2.59

Guided Bone Regeneration in Comminuted Long-Bone Fractures Using Recombinant Human Bone Morphogenetic Protein-2 and a Collagen Membrane  

Jang, Kwangsik (Biomaterial R&BD Center, Chonnam National University)
Jo, Hyun Min (Biomaterial R&BD Center, Chonnam National University)
Shim, Kyung Mi (Biomaterial R&BD Center, Chonnam National University)
Kim, Se Eun (Biomaterial R&BD Center, Chonnam National University)
Kang, Seong Soo (Biomaterial R&BD Center, Chonnam National University)
Publication Information
Journal of Veterinary Clinics / v.39, no.2, 2022 , pp. 59-64 More about this Journal
Abstract
A dog aged two years and seven months and a cat aged seven years were referred owing to fractures of long bones. Preoperative radiographs revealed comminuted bone fractures close to joints. Conventionally, long-bone fractures are treated using intramedullary pins, plate and screw systems, or an external fixator system. In cases of non-reducible fractures, various graft materials have been used in fracture treatments to stimulate bone repair. Here, recombinant human bone morphogenetic protein-2 (rhBMP-2) and a collagen membrane were applied. Four weeks after surgery, fractured bone fragments began to unite and the bone union was observed using radiography four months after surgery. No complications occurred related to grafted materials. We successfully applied rhBMP-2 and collagen membranes in two different species to support the healing process of comminuted fractures, according to the concept of guided bone regeneration.
Keywords
comminuted long bone fracture; rhBMP-2; collagen membrane; guided bone regeneration; animal;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Tal H, Moses O, Zohar R, Meir H, Nemcovsky C. Root coverage of advanced gingival recession: a comparative study between acellular dermal matrix allograft and subepithelial connective tissue grafts. J Periodontol 2002; 73: 1405-1411.   DOI
2 Ferreira AM, Gentile P, Chiono V, Ciardelli G. Collagen for bone tissue regeneration. Acta Biomater 2012; 8: 3191-3200.   DOI
3 Guda T, Walker JA, Singleton BM, Hernandez JW, Son JS, Kim SG, et al. Guided bone regeneration in long-bone defects with a structural hydroxyapatite graft and collagen membrane. Tissue Eng Part A 2013; 19: 1879-1888.   DOI
4 Harwood PJ, Giannoudis PV. Application of bone morphogenetic proteins in orthopaedic practice: their efficacy and side effects. Expert Opin Drug Saf 2005; 4: 75-89.   DOI
5 Huntingford JL. Fundamentals of physical rehabilitation. In: Fossum TW, Duprey LP, editors. Small animal surgery. 5th ed. Philadelphia: Elsevier. 2019: 105-109.
6 James AW, LaChaud G, Shen J, Asatrian G, Nguyen V, Zhang X, et al. A review of the clinical side effects of bone morphogenetic protein-2. Tissue Eng Part B Rev 2016; 22: 284-297.   DOI
7 Khojasteh A, Kheiri L, Motamedian SR, Khoshkam V. Guided bone regeneration for the reconstruction of alveolar bone defects. Ann Maxillofac Surg 2017; 7: 263-277.   DOI
8 Lee CS, Bentley RT, Weng HY, Breur GJ. A preliminary evaluation of the reliability of a modified functional scoring system for assessing neurologic function in ambulatory thoracolumbar myelopathy dogs. BMC Vet Res 2015; 11: 241.   DOI
9 Xue J, He M, Niu Y, Liu H, Crawford A, Coates P, et al. Preparation and in vivo efficient anti-infection property of GTR/GBR implant made by metronidazole loaded electrospun polycaprolactone nanofiber membrane. Int J Pharm 2014; 475: 566-577.   DOI
10 Arik YB, de Sa Vivas A, Laarveld D, van Laar N, Gemser J, Visscher T, et al. Collagen I based enzymatically degradable membranes for organ-on-a-chip barrier models. ACS Biomater Sci Eng 2021; 7: 2998-3005.   DOI
11 Caballe-Serrano J, Sawada K, Miron RJ, Bosshardt DD, Buser D, Gruber R. Collagen barrier membranes adsorb growth factors liberated from autogenous bone chips. Clin Oral Implants Res 2017; 28: 236-241.
12 Binsalah MA, Ramalingam S, Alkindi M, Nooh N, Al-Hezaimi K. Guided bone regeneration of femoral segmental defects using equine bone graft: an in-vivo micro-computed tomographic study in rats. J Invest Surg 2019; 32: 456-466.   DOI
13 Chen NF, Smith ZA, Stiner E, Armin S, Sheikh H, Khoo LT. Symptomatic ectopic bone formation after off-label use of recombinant human bone morphogenetic protein-2 in transforaminal lumbar interbody fusion. J Neurosurg Spine 2010; 12: 40-46.   DOI
14 Sheikh Z, Qureshi J, Alshahrani AM, Nassar H, Ikeda Y, Glogauer M, et al. Collagen based barrier membranes for periodontal guided bone regeneration applications. Odontology 2017; 105: 1-12.   DOI
15 Hayashi K, Schulz KS. Principles of fracture diagnoses and management. In: Fossum TW, Duprey LP, editors. Small animal surgery. 5th ed. Philadelphia: Elsevier. 2019: 1023-1035.
16 Almazrooa SA, Noonan V, Woo SB. Resorbable collagen membranes: histopathologic features. Oral Surg Oral Med Oral Pathol Oral Radiol 2014; 118: 236-240.   DOI
17 Fillingham Y, Jacobs J. Bone grafts and their substitutes. Bone Joint J 2016; 98-B(1 Suppl A): 6-9.   DOI
18 Jeong KJ, Yang E, Jang K, Shim KM, Bae CS, Kim SE, et al. Successful clinical application of cancellous allografts with structural support for failed bone fracture healing in dogs. In Vivo 2019; 33: 1813-1818.   DOI
19 Lu S, Zhang Z, Wang J. Guided bone regeneration in long bone. An experimental study. Chin Med J (Engl) 1996; 109: 551-554.
20 Turri A, Elgali I, Vazirisani F, Johansson A, Emanuelsson L, Dahlin C, et al. Guided bone regeneration is promoted by the molecular events in the membrane compartment. Biomaterials 2016; 84: 167-183.   DOI