• International journal of advanced smart convergence
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• 제7권1호
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• pp.42-47
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• 2018

Recently, as the demand for limited resources continues to rise and problems of resource depletion rise worldwide, the importance of renewable energy is gradually increasing. In order to solve these problems, various methods such as energy conservation and alternative energy development have been suggested, and biogas, which can utilize the gas produced from biomass as fuel, is also receiving attention as the next generation of innovative renewable energy. New and renewable energy using biogas is an energy production method that is expected to be possible in large scale because it can supply energy with high efficiency in compliance with energy supply method of recycling conventional resources. In order to more efficiently produce and manage these biogas, a biogas plant has emerged. In recent years, a large number of biogas plants have been installed and operated in various locations. Organic wastes corresponding to biogas production resources in a biogas plant exist in a wide variety of types, and each of the incoming raw materials is processed in different processes. Because such a process is required, the case where the biogas plant process is inefficiently operated is continuously occurring, and the economic cost consumed for the operation of the biogas production relative to the generated biogas production is further increased. In order to solve such problems, various attempts such as process analysis and feedback based on the feedstock have been continued but it is a passive method and very limited to operate a medium/large scale biogas plant. In this paper, we propose "CNN-based production yield prediction algorithm for increasing process efficiency of biogas plant" for efficient operation of biogas plant process. Based on CNN-based production yield forecasting, which is one of the deep-leaning technologies, it enables mechanical analysis of the process operation process and provides a solution for optimal process operation due to process-related accumulated data analyzed by the automated process.

• KSBB Journal
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• 제20권1호
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• pp.34-39
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• 2005

목련과 수종인 함박꽃나무의 현탁배양세포로부터 생리활성을 갖는 리그난화합물인 (+)-eudesmin을 효율적으로 생산하기 위한 연구로써 플라스크배양 단계에서의 다양한 배양조건들 즉, 배지, 초기 당농도, 교반속도, 초기 접종농도, 그리고 elicitation 효과를 확인하고자 하였다. MS배지를 포함한 4종의 배지에서는 물질의 생산성과 생중량 모두에서 MS배지가 적합한 것으로 나타났다. 130 rpm으로 교반되는 항온배양기에서 $3\%$ sucrose와 0.5 mg/L 2,4-D가 첨가된 MS배지에 0.5 mg (DCW)의 농도로 세포를 접종한 실험구에서 8주 후 플라스크 당 3.71 g (DCW)의 생중량을 얻었으며, 지표물질인 (+)-eudesmin의 함량은 $5\%$ sucrose와 200 mg/L chitosan 처리구에서 $3.2{\mu}g/g$ (DCW)으로 대조구에 비해 1.7배의 증가를 나타내었다. 이와 같은 연구결과는 생물반응기를 이용한 목련과 수종에서의 유용물질 생산 연구에 활용될 수 있을 것으로 사료된다.

• KSBB Journal
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• 제21권2호
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• pp.123-128
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• 2006

황기의 형질전환된 모상근으로부터 약리물질인 astragalosides I, II, 그리고 III을 효율적으로 생산하기 위하여 다양한 물리화학적 조건들 즉, 배지, 초기 당농도, 교반속도, 초기 접종농도, 광조사, 그리고 chitosan 처리에 한 elicitation 효과를 조사 하였다. 선발된 AG-04 clone의 성장과 astragalosides의 함량은 4종의 배지들 중 SH배지에서 가장 좋았으며, 배지에 첨가하는 초기 당 농도는 3%와 6%에서 각기 건물중과 astragalosides의 함량이 가장 높게 나타났다. 또한, 초기 접종량은 50 ml 배지에 500 mg(FRW)씩을 주입하는게 가장 효과적이었으며, 18시간 광조사가 되는 Shaking incubator에서 90 rpm과 120으로 교반시켰을 때 모상근의 성장과 astargalosides의 함량이 높게 나타났다. 배양 2주 후 chitosan의 처리는 모상근의 성장에는 크게 영향을 미치지 않았으나, 100 mg/L 처리구에서 대조구의 약 2.1배의 astragalosides 함량 증가를 가져왔다. 이와같은 연구결과는 황기의 대량배양을 통한 saponins 생산 연구에 효율적으로 활용될 수 있을 것으로 본다.

• 한국대기환경학회지
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• 제27권4호
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• pp.426-435
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• 2011

In this study, PM emission factors for commercial meat cooking were developed by the experiment using the similar restaurant model. The commercial material types of meat cooking were beef, pork and duck. And meat cooking materials were classified with marinated and raw cooking methods. The marinated and raw cooking methods were flat griddle (LPG) and under fired charbroiling grill. As a results, $PM_{10}$ emission factors for underfired pork cooking was estimated as 7.39 g/kg-meat which was the highest value in this study. The emission factors of under fired charbroiling grill method were higher than that of flat griddle method regardless of meat cooking material types and marinated meat. The particle size distribution of meat cooking was 0.23~5 ${\mu}m$ and median diameter was 2~2.5 ${\mu}m$.

• 한국지하수토양환경학회지:지하수토양환경
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• 제22권6호
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• pp.94-103
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• 2017

Surface soil is one of the most important resources that have many functions for human needs such as conservation of water resource, purification of contaminated materials, and productivity of food or energy. However, the surface soil is a limited resource that cannot be recovered readily for a long time once it is lost by erosion. In Korea, the Ministry of Environment enacted the notification on the investigation of surface soil erosion and corresponding countermeasures. As the results, database of soil quality assess criteria (biomass, groundwater recharge, habitat, carbon storage, buffer, and soil loss) was established, and the web-based system that can evaluate surface soil conditions was developed. However, non-experts have difficulties in using the system because the system requires in-depth knowledge about soil qualities. In this study, the Web Geographic Information System (GIS) watershed-based surface soil information system was developed to improve usability of the system and accessibility of soil quaility database. The system provides the current condition of surface soil characteristics and GIS-based soil data at selected locations. The users are able to download soil quality data in different districts, watersheds, and special regions allocated by TauDEM module. The system developed in this study would valuable surface soil information for studies of soil quality and its environmental effects, and thereby contributing to establishing more appropriate and robust soil conservation laws.

• 한국약용작물학회지
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• 제14권1호
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• pp.31-35
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• 2006

Agrobacterium rhizogenes ATCC15834에 의해 형질전환된 지황 (Rehmannia glutinosa)의 모상근으로부터 유식물체를 재분화 시키기 위한 최적 조건을 탐색하였다. 모상근 절편으로부터 유식물체의 재분화는 0.5 mg/l BA가 첨가된 SH배지 (3% sucrose, pH 5.8)에서 배양 5주 후 이루어졌다. 이와 같은 유식물체는 식물생장조절물질이 포함되지 않은 기본배지로 옮겼을 때 발근이 이루어졌다. 형질전환된 모상근 유래 식물체는 잎으로부터 직접 재분화된 식물체와 비교했을 때 잎이 오므라드는 경향을 보였고 줄기는 절간이 짧았으며, 뿌리는 가늘고 수량이 많은 특징을 보였다. 생체량 비교에서 형질전환식물체는 식물 하나의 무게가 1.28 g (생중량)으로 대조구인 잎 유래 재분화식물체 (0.53 g)에 비해 높았으며, 지표물질인 catalpol의 함량에 있어서도 차이를 보여주었다.

• ALGAE
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• 제33권3호
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• pp.279-290
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• 2018

Microalgae have been utilized in diverse industries including aquaculture. Among the microalgae, dinoflagellates are known to have various bioactive compounds, and thus the interest in their application to industry has increased. In order to test their potential as food materials for aquaculture animals, the crude protein contents and compositions of amino acids of six dinoflagellates Heterocapsa rotundata (family Heterocapsaceae), Ansanella granifera (Suessiaceae), Alexandrium andersonii (Ostreopsidaceae), Takayama tasmanica (Brachidiniaceae), Takayama helix, and Gymnodinium smaydae (Gymnodiniaceae) belonging to diverse families were analyzed. The percentage of the amount of the crude protein relative to dry weight of T. tasmanica was the highest (65%) and that of A. andersonii was the lowest (26%). However, the highest percentage of total detected amino acids in crude protein was found in A. andersonii (98.2%). In all six dinoflagellates, glutamic acid was the most dominant amino acid in crude protein. However, the second main amino acid was aspartic acid for H. rotundata, A. granifera, T. helix, and G. smaydae, but were arginine and leucine for A. andersonii and T. tasmanica, respectively. Furthermore, T. tasmanica and T. helix did not have taurine and gamma-aminobutyric acid, whereas the other dinoflagellates possessed them. The percentages of essential amino acid contents of the dinoflagellates met the requirement levels for juvenile shrimps. In addition, the dinoflagellates were not toxic to the brine shrimp Artemia salina. Compared with the other microalgae reported so far, H. rotundata and A. andersonii can be used for arginine-rich diets, T. tasmanica for valine and leucine-rich diets, A. granifera for histidine-rich diets, T. helix for threonine-rich diets, and G. smaydae for lysine-rich diets. Therefore, based on their biochemical composition and toxicity to Artemia, the dinoflagellates could be used as essential amino acid sources for cultivating animals in the aquaculture industry.

• 한국약용작물학회지
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• 제26권6호
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• pp.464-470
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• 2018

Background: Ginseng produced by hydroponics can be cultivated without using agricultural chemicals; thus, it can be used as a raw materials for functional foods, medicines, and cosmetics. This study aimed to determine the optimal harvesting time to obtain the highest levels of ginsenoside and ginseng, as this was not previously unknown. Methods and Results: One-year-old organic ginseng seedlings were transplanted and cultivated using hydroponics for 150 days in a venlo-type greenhouse, using ginseng nursery bed soil and a nutrient solution ($NO_3{^-}-N$; 6.165, P; 3.525, K; 5.625, Ca; 4.365, Mg; 5.085, S; $5.31mEq/{\ell}$). Ginsenoside content and fresh and dry weights were higher at 120 days after transplanting than at 30, 60, 90, and 150 days. Total ginsenoside content was 11.86 times higher in the leaf and stem than in the root at 120 days after transplanting. Ginsenosides F1, F2, F3, and F5 were detected in ginseng leaves and stems. These chemical compounds are known to be effective in altering skin properties, including whitening, anti-inflammation, and anti-aging. Conclusions: Optimal harvesting time for ginseng cultivated using hydroponics was 120 days after transplanting when the biomass and ginsenoside content were highest.

• 한국약용작물학회지
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• 제28권6호
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• pp.445-454
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• 2020

Background: Culturing wild ginseng adventitious root using plant factory technology provides genetic safety and high productivity. This production technology is drawing attention in the fields of functional raw materials and product development. The cultivation method using elicitors is key technology for controlling biomass and increasing secondary metabolites. Methods and Results: Elicitor treatments using methyl jasmonate, pyruvic acid, squalene, β-sistosterol were performed to amplify total ginsenosides (Rb1, Rc, Rb2, Rb3, and Rd) of cultured wild ginseng adventitious root. Thereafter, fermentation and steaming processes were performed to convert total ginsenosides into minor molecular ginsenosides (Rg3, Rk1, and Rg5). The result indicated that methyl jasmonate minimizes the reduction in fresh weight of cultured wild ginseng adventitious root and maximizes total ginsenosides (sum of Rb1, Rc, Rb2, Rb3, and Rd). Ginsenoside conversion results showed a maximum degree of conversion of 131 mg/g. Conclusions: In this study, we demonstrated that the optimal elicitor treatment method increased the content of total ginsenosides, while the steaming and fermentation processing method increased the content of minor ginsenosides.

• 한국식생활문화학회지
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• 제36권1호
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• pp.66-75
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• 2021

Microalgae are unicellular microorganisms inhabiting various ecosystems of the world, including marine and freshwater systems and extreme environments. Only a few species have been actively used as food. Microalgae are attracting attention as a means of biological CO2 reduction because they play an important role in absorbing atmospheric CO2 through their rapid growth by photosynthesis in water. Besides, microalgae are considered to be an eco-friendly energy source because they can rapidly produce biomass containing a large quantum of lipids that can be converted into biodiesel. Several microalgae, such as Chlorella spp., Spirulina spp. and Haematococcus spp. have already been commercialized as functional health supplements because they contain diverse nutrients including proteins, vitamins, minerals, and functional substances such as docosahexaenoic acid (DHA), β-glucan, phycocyanin, astaxanthin, etc. Moreover, they have the potential to be used as food materials that can address the protein-energy malnutrition (PEM) which may occur in the future due to population growth. They can be added to various foods in the form of powder or liquid extract for enhancing the quality characteristics of the foods. In this review, we analyzed several microalgae which can be used as food additives and summarized their characteristics and functions that suggest the possibility of a role for microalgae as future food.