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Optimization of Culture Conditions for the [+]-Eudesmin Production in Magnolia Sieboldii Cells  

Hwang Sung Jin (Department of Oriental Medicine Materials, Dongshin University)
Publication Information
KSBB Journal / v.20, no.1, 2005 , pp. 34-39 More about this Journal
Abstract
In order to product the furofuranoid lignans, (+)-eudesmin which is one of the secondary products from Magnolia sieboldii. through cell suspension cultures; various culture media, initial sucrose concentration, elicitations, shaking speeds, and inoculum sizes. Among the culture media tested, MS medium had a pronounced effect on suspension cell growth and (+)-eudesmin contents. The maximum dry cell weight (DCW) of 3.71 g per flask was obtained at inoculum size of 0.5 g and in MS medium supplemented with $3\%$ sucrose plus 0.5 mg/L 2,4-D after 8 weeks. (+)-Eudesmin biosynthesis was stimulated with high initial sucrose concentration ,and the maximum (+)-eudesmin production of $3.2{\mu}g/g$ DCW was achieved at 200mg/L chitosan and $5\%$ initial medium sucrose. The optimal shaking speeds for dry biomass accumulation and (+)-eudesmin contents was 130 rpm. This work is considered to be helpful for large-scale bioprocessing of Magnolia sieboldii suspension cell cultures in bioreactor.
Keywords
Magnolia sieboldii; suspension cell cultures; (+)-eudesmin;
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1 Kvasnickova, L., Z. Glatz, H Sterbova, V. Kahle, J. Slanina ,and P. Musil (2001), Application of capillary electrochromatography using macroporous polyacrylamide columns for the analysis of lignanas from seeds of Schisandra chinensis, J. Chromatogr. A 916, 265-271   DOI   PUBMED   ScienceOn
2 Sladkovsky, R., P. Solich, and L. Opletal (2001), Simultaneous determination of quercetin, kaempferol and (E)-cinamic acid in vegetative organs of Schisandra chinensis by HPLC, J. Pharm. Biochemi. Anal. 24, 1049-1054   DOI   ScienceOn
3 Paska, C., G. Innocenti, M. Kunvari, M. Laszlo, and M. Szilagyi (1999), Lignan production by Ipomoea cairica callus cultures, Phytochemistry 52, 879-883   DOI   ScienceOn
4 Park, H. J. (1996), A new aporphine-type alkaloid from the leaves of Magnolia sieboldii, Korean J. Pharmacogn. 27, 123-128
5 Mori, T., M. Sakurai, and S. Furusaki (1994), Effects of conditioning factor on anthocyanin production in strawberry suspension cultures, J. Sci. Food Agric. 66, 381-388   DOI   ScienceOn
6 Berlin, J. E. Forche, V. Wray, J. Hammer, and W. Hosel (1983), Formation of benzophenantltiridine alkaloids by suspension cultures of Eschscholtzia californica, Z. Naturforsch 38, 346-352
7 Sakamoto, K., K. Iida, K. Sawamura, K. Hajiro, Y. Asada, T. Yoshikawa, and T. Furuya (1993), Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata, Phytochemicals 33, 357-360   DOI   ScienceOn
8 Zhong, J. J. and T. Yoshida (1995), High-density cultivation of Perilla frutescens cell suspensions for anthocyanin production: Effects of sucrose concentration and inoculum size, Enzyme Microb. Technol. 17, 1073-1079   DOI   ScienceOn
9 Su, W. W. and F. Lei (1993), Rosmarinic acid production in perfused Anchusa officinalis cultures: Effects of inoculum size, Biotechnol. Lett. 15, 1035-1038   DOI   ScienceOn
10 Wu, S., Y. Zu, and M. Wu (2003), High yield production of salidroside in the suspension culture of Rhodiola sachalinensis, J. Biotechnol. 106, 33-43   DOI   ScienceOn
11 Gregorio, G. H. and V. M. Loyola-vargas (1997), Effects of ASA on secondary metabolism of C. roseus tumor suspension culture, Plant Cell Rep. 16, 87-290
12 Lloyd, G. B. and B. H. McCown (1980), Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by the use of shoot top culture, Comb. Proc. Int. Plant Prop. Soc. 30, 421-437
13 Miyauchi, T. and S. Ozawa (1998), Formation of (+)-eudesmin in Magnolia kobus, Phytochemistry 47, 665-670   DOI   ScienceOn
14 Knobloch, K. H. and J. Berlin (1980), Influence of medium composition on the formation of secondary compounds in cell suspension cultures of Catharanthus roseus, Z. Naturforsch 35, 551-556
15 Petersen, M. and A. W. Alfermann (2001), The production of cytotoxic lignans by plant cell cultures, App. Microbiol. Biotechnol. 55, 135-142   DOI   ScienceOn
16 Rao, S. R. and G. A. Ravishankar (2002), Plant cell culture: Chemical factories of secondary metabolites, Biotechnology Advances 20, 101-153   DOI   ScienceOn
17 Dornenberg, H. and D. Knorr D (1995), Strategies for the improvement of secondary metabolites production in plant cell cultures. Enzyme Microb. Technol. 17, 674-684   DOI   ScienceOn
18 Fujita, M., H. Itokawa, and Y. Sashida (1972), Honokiol, a new phenolic compound isolated from the bark of Magnolia ovata, Chem. Pharm. Bull. 20, 183-217
19 Stuart, R. and H. E. Street (1969), Studies on the growth in culture of plant cells. IV. The initiation of division in suspensions of stationary phase cells of Acer pesudoplatanus L, J. Exp Bot 20, 556-571   DOI
20 Matsubara, K., K. Shigekazu, T. Yoshioka, T. Morimoto, Y. Fujita, and Y. Yamada (1989), High density culture of Coptis japonica cells increases berberine production, J. Chem. Tech Biotech 46, 61-69   DOI
21 Misawa, M. (1985), Production of useful plant metabolites. In Adv Biochem Eng Biotechnol. A. Fiechter Eds., pp. 59-88, Berlin, Springer-Verlag
22 Smollny, T., H. Wichers, S. Kalenberg, A. Shahsavari, M. Petersen, and A. W. Alfennann (1998), Accumulation of podophyllotoxin and related lignans in cell suspension cultures of Linum album, Phytochemistry 48, 975-979   DOI   ScienceOn
23 Lim, S. S., K. H. Shin, H. S. Ban, Y. P. Kim, S. H. Jung, Y. J. Kim, and K. Okuchi (2002), Effect of the essential oil from the flowers of Magnolia sieboldii on the lipopolysaccharide-induced production of nitric oxide and prostaglandin E2 by rat peritoneal macrophage, Plant Med. 68, 459-462   DOI   ScienceOn
24 DiCosmo, F. and G. H. N. Towers (1984), Stress and secondary metabolism in .cultured plant cells. In Phytochemical adaptations to stress , B. N. Tinunermann, .C. Steelink and F. A. Loewus, Eds., p97-175, Plenum press, NY
25 Van Gulik, W. M., A. M. Nuutila, K. L. Vinke, H. J. G. ten Hoopen, and J. J. Heijnen (1994), Effects of carbon dioxide, air flow rate, and inoculum density on the batch growth of Catharanthus roseus cell suspension in stirred ferments, Biotechnol. Prog. 10, 335-339   DOI   ScienceOn
26 Zhong, J. J., J. Seki, S. Kinoshita, and T. Yoshida (1992), Physiological characteristics of cell suspension and cell culture of Perillar frutescens, Biotechnol. Bioeng. 40, 1256-1262   DOI   PUBMED
27 Chang, J. H. and H. J. Lee (1992), Characteristics of oil production during suspension culture with chitosan elicitation, Ann Res Center for New Bio-Master Agric. 111-117
28 Choi, J. H., J. Ha, J. H. Park, J. Y. Lee, Y. S. Lee, H. J. Park, J. W. Choi, K. Mass Nakaya, and K.T Lee (2002), Costunolide triggers apoptosis in human leukemia U937 by depleting intracellular thiols. Japan J. Cancer Res. 93, 1327-1333   DOI
29 Murashige, T. and F. Skoog (1969), A revised medium for rapid growth and bioassay with tobacco tissue cultures, Physiol. Plant. 15, 473-497   DOI
30 Sakato, K. and M. Misawa (1974), Effects of chemical and physical conditions on growth of Campotheca acuminata cell cultures, Agric. Biol. Chem. 38, 491-498   DOI
31 Funk, C. and P. Brodelius (1990), Influence of growth regulators and an elicitor on phenylpropanoide metabolism in suspension cultures of Vanilla planifolia, Phytochemistry 29, 845-848   DOI   ScienceOn
32 Sakurai, M. and T. Mori (1996), Stimulation of anthocyanin synthsis by conditioned medium produced by strawberry suspension cultures, J. Plant Physiol. 149, 599-604   DOI   ScienceOn
33 Do, C. B. and F. Cormier (1999), Accumulation of anthocyanins enhanced by a high osmotic potential in grape cell suspension culture, Plant Cell Rep. 9, 143-146
34 Ulbric, B., W. Wiesner, and H. Arens (1985), Large scale production of rosmarinic acid from plant cell cultures of Coleus blumei Benth. In Secondary metabolism oj plant cell culture, B. Deus-Neumann, W. Barz and E. Reinhard Eds.; Berlin, Springer-Verlag pp. 293-303
35 Kimura, M., J. Suzuki, M. Yamada, M. Yoshizaki, T. Kikuchi, S. Kadota, and S. Masuda (1985), Antiinflammatory effect of neolignans isolated from the crude drug 'Shin-i'(Flos Magnoliae), Planta Med 51, 291-293   DOI   ScienceOn
36 Seidel, V., J. Windhovel, G. Eaton, A. W. Alferrnann, R. R. J. Arroo, M. Medarde, M. Petersen, and J. G. Woolley (2002), Biosynthesis of podophyllotoxin in Linum album cell cultures, Planta 215, 1031-1039   DOI   ScienceOn
37 Van Uden, W., N. Pras, and T. M. Malingre (1990), On the improvement of the podophyllotoxin production by phenylpropanoid precursor feeding to cell cultures of Podophyllum hexandrum, Plant Cell Tissue Org. Cult. 23, 217-224   DOI
38 Park, S. H., S. U. Choi, C. O. Lee, S. E. Yoo, S. K. Yoon, Y. K. Kim, and S. Y. Ryu (2001), Costunolide, a sesquiterpene from the stem bark of Magnolia sieboldii, inhibits the RAS-famesyl-proteintransferase, Plant Med. 67, 358-359   DOI   ScienceOn
39 Endress, R. (1994), Plant cell biotechnology, p121-242, SpringerVerlag, Berlin, Heidelberg
40 Gamberg, O. L., R. A. Miller, and K. Ojima (1968), Nutrient requirements of suspension cultures of soybean root cells, Exp. Cell Res. 50, 148-151
41 James, P. K., M. D. Samija, G. M. Hewirr, E. C. Bugante, and H. Gu (1993), Antiinflammatoryoleanane triterpenes from Terygium wilfordii cell suspension cultures by fungal elicitation, Plant Cell Rep. 12, 356-359
42 Schenk, R. V. and A. C. Hildrbrandt (1972), Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures, Can J. Bot. 50, 199-204   DOI