Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Plant Regeneration from Hairy Root of Rehmannia glutinosa Liboschitz Transformed by Agrobacterium rhizogenes

형질전환된 지황의 모상근으로부터 식물체의 재분화

  • Hwang, Sung-Jin (Department of Oriental Medicine Materials, Dongshin University)
  • 황성진 (동신대학교 한약재산업학과)
  • Published : 2006.02.28
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Abstract

A protocol for plant regeneration from hairy root of Rehmannia glutinosa transformed by Agrobacterium rhizogenes ATCC15834 has been developed. Transgenic shoots were regenerated from hairy roots within 6 weeks after culture on the SH medium supplemented with 0.5 mg/l BA. Shoots were rooted on plant growth regulator free SH medium successfully. The transformed plants, which were regenerated from hairy roots, had thiner roots with extensive lateral branches, wrinkled leaves, shorter node, and grew faster compared with non-transformed plants. The biomass of the transformed plant was 1.28 g (F.W) per plant, significantly higher than the non-transformed plant (0.54 g F.W). The catalpol content in the transformed plant (0.56%) was also higher than that of the non-transformed plants (0.43%).

Agrobacterium rhizogenes ATCC15834에 의해 형질전환된 지황 (Rehmannia glutinosa)의 모상근으로부터 유식물체를 재분화 시키기 위한 최적 조건을 탐색하였다. 모상근 절편으로부터 유식물체의 재분화는 0.5 mg/l BA가 첨가된 SH배지 (3% sucrose, pH 5.8)에서 배양 5주 후 이루어졌다. 이와 같은 유식물체는 식물생장조절물질이 포함되지 않은 기본배지로 옮겼을 때 발근이 이루어졌다. 형질전환된 모상근 유래 식물체는 잎으로부터 직접 재분화된 식물체와 비교했을 때 잎이 오므라드는 경향을 보였고 줄기는 절간이 짧았으며, 뿌리는 가늘고 수량이 많은 특징을 보였다. 생체량 비교에서 형질전환식물체는 식물 하나의 무게가 1.28 g (생중량)으로 대조구인 잎 유래 재분화식물체 (0.53 g)에 비해 높았으며, 지표물질인 catalpol의 함량에 있어서도 차이를 보여주었다.

Keywords

References

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