• Title/Summary/Keyword: Biomass Measurement

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Eco-friendly Leather Dyeing using Biomass Wastes(I): Natural Dyeing of Eel Skin using Onion Peels (생물자원 폐기물을 활용한 친환경 가죽염색(I): 양파껍질을 이용한 장어가죽 천연염색)

  • Yeo, Youngmi;Yoo, Dong Il;Shin, Younsook
    • Textile Coloration and Finishing
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    • v.30 no.2
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    • pp.141-149
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    • 2018
  • This study aimed to investigate the efficacy of eco-friendly leather dyeing by utilizing food wastes. Natural dyeing of eel skin was attempted using onion peels which have been used commonly for natural dyeing of textile fabrics. Eel skin is a by-product from fishery processing and is used mainly for making leather products. The colorant was extracted from onion peels in boiling water, concentrated, and freeze-dried. Dyeing of eel skin was carried out to study the effects of dyeing conditions, mordant type and mordanting method on dye uptake, color change, drape stiffness and colorfastness. The optimum dyeing conditions were $60^{\circ}C$ of dyeing temperature, 60min of dyeing time at 1:100($H_2O$ 90%: ethanol 10%) of bath ratio. The onion peels produced yellowish color on eel skin. The pre-mordanting was effective than the post-mordanting. As a result of the drape stiffness measurement, the Fe-mordanted sample was somewhat stiffer comparing to other mordanted samples. The light fastness of the non-mordant dye was excellent in 3-4 grade. Drycleaning fastness and rubbing fastness showed excellent results, but fastness was not significantly improved by mordanting.

Application of a Fed-Batch Bioprocess for the Heterologous Production of hSCOMT in Escherichia coli

  • Passarinha, L.A.;Bonifacio, M.J.;Queiroz, J.A.
    • Journal of Microbiology and Biotechnology
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    • v.19 no.9
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    • pp.972-981
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    • 2009
  • In this paper, a fed-batch cultivation process in recombinant Escherichia coli BL21(DE3) bacteria, for the production of human soluble catechol-O-methyltransferase (hSCOMT), is presented. For the first time, a straightforward model is applied in a recombinant hSCOMT expression system and distinguishes an initial cell growth phase from a protein production phase upon induction. Specifically, the kinetic model predicts biomass, substrate, and product concentrations in the culture over time and was identified from a series of fed-batch experiments designed by testing several feed profiles. The main advantage of this model is that its parameters can be identified more reliably from distinct fed-batch strategies, such as glycerol pulses and exponential followed by constant substrate additions. Interestingly, with the limited amount of data available, the proposed model accomplishes satisfactorily the experimental results obtained for the three state variables, and no exhaustive process knowledge is required. The comparison of the measurement data obtained in a validation experiment with the model predictions showed the great extrapolation capability of the model presented, which could provide new complementary information for the COMT production system.

Fermentation Characteristics and Microbial Protein Synthesis in an In Vitro System Using Cassava, Rice Straw and Dried Ruzi Grass as Substrates

  • Sommart, K.;Parker, D.S.;Rowlinson, P.;Wanapat, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.8
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    • pp.1084-1093
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    • 2000
  • An in vitro gas production system was used to investigate the influence of various substrate mixtures on a natural mix of rumen microbes by measurement of fermentation end-products. The treatments were combinations of cassava (15.0, 30.0 and 45.0%) with different roughage sources (ruzi grass, rice straw or urea treated rice straw). Microbial biomass, net $^{15}N$ incorporation into cells, volatile fatty acid production, gas volume and rate of gas production increased linearly with increasing levels of cassava inclusion. There was also an effect of roughage source, with rice straw being associated with the lowest values for most parameters whilst similar values were obtained for ruzi grass and urea treated rice straw. The results suggest that microbial growth and fermentation rate increase as a function of readily available carbohydrate in the substrate mixture. A strong linear relationship between $^{15}N$ enrichment, total volatile fatty acid production and gas production kinetics support the suggestion of the use of the in vitro gas production system as a tool for screening feedstuffs as an initial stage of feed evaluation.

Mass Cultivation of Botryococcus braunii for the Advanced Treatment of Swine Wastewater and Lipid Production in a Photobioreactor (축산폐수의 고도처리 및 지질생산을 위한 Botryococcus branuii의 대량배양)

  • 이석준;김성빈;김희식;권기석;윤병대;오희목
    • Microbiology and Biotechnology Letters
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    • v.27 no.2
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    • pp.166-171
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    • 1999
  • This study was conducted to investigate the removal rate of nitrogen and phosphorus, and lipid production from a swine wastewater by Botryococcus braunii UTEX 572 in an outdoor photobioreactor. B. braunii successfully predominated in competition with bacteria and other algae, especially Oscillatoria, which were grown spontaneously in a secondary-treated swine wastewater, under the conditions of incubation temperature at $25^{\circ}C$ and increased inoculum amount at 287mg/l. There was a significant relationship between dry weight of B. braunii and absorbance of culture solution at 680mn(r2=0.967), suggesting that the latter is as good as the former commonly used for the measurement of algal biomass which is considerably time-consuming. The removal rates of COD, TOC, total nitrogen, and total phosphorus from the swine wastewater were 33.2$\pm$2.6% and 32.8$\pm$3.2, respectively, which showing no different between them. These results suggested that the mass cultivation of B. braunii in an outdoor photobioreactor could be used for the advanced treatment of swine wastewater and lipid production.

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Hydrophilic Extracts of the Bark from Six Pinus Species

  • Masendra, Masendra;Ashitani, Tatsuya;Takahashi, Koetsu;Susanto, Mudji;Lukmandaru, Ganis
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.1
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    • pp.80-89
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    • 2019
  • Pine barks are important biomass resources because they are utilised in the production of pine wood and rosins. However, no chemical study has been conducted on the hydrophilic status of pine barks in Indonesia. This aim of this study is to explore the hydrophilic extracts of the barks from six Pinus species (P. elliotii, P. caribeae, P. oocarpa, P. merkusii P. montezumae, and P. insularis). The hydrophilics of pine barks were analysed using gas chromatography-mass spectrometry. The presence of polyphenol contents in the ethanol extracts obtained from the barks of six Pinus species was determined using the tannin-formaldehyde method, Folin-Cioucalteu assay, and vanillin-HCl assay. The ethanol and hot water soluble extractives derived from inner barks were higher in quantity when compared to those derived from the outer bark samples. The polyphenol measurement showed that the highest value of total phenol content was derived from the outer bark of P. montezumae whereas those of the total phenol and tannin- formaldehyde contents were derived from the inner and outer barks of P. oocarpa. GC-MS analysis revealed that nitrogenous compounds are dominant constituents in the inner and outer barks of the six species, followed by sugars and monophenolics, respectively.

Ecosysteme de I′Etang de Berre (Mediterranee nord-occidentale) : Caracteres Generales Physiques, Chimiques et Biologiques

  • Kim, Ki-Tai
    • Korean Journal of Environmental Biology
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    • v.22 no.2
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    • pp.247-258
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    • 2004
  • Climatological, hydrological and planktonical research studies, measurements of primary production and photosynthetic efficiency from December 1976 to December 1978 have been carried out in two brackish lakes: Lake Etang de Berre and Lake Etang de Vaine located in the French Mediterranean coast, in the region of Carry-le-Rouet located on the north-west Mediterranean near Marseilles, and in fresh water inflows from 4 Rivers (Touloubre, Durance, Arc, Durancole) to Lake Etang de Berre. Physico-chemical parameters were measured for this study: water temperature, salinity, density, pH, alcalinity, dissolved oxygen (% saturation), phosphate, nitrate, nitrite, silicate etc. Diverse biological parameters were also studied: photosynthetic pigments, phaeopigments, specific composition and biomass of phytoplankton, primary pelagic production etc. Climatical factors were studied: air-temperature, solar-radiation, evaporation, direction (including strength) of winds, precipitation and freshwater volume of the four rivers. The changes in Lake ‘Etang de Berre’ ecosystem depend on the quality of the water in the Durance River, and on the effects of seawater near the entrance of the Caronte Canal. The water quality of the lake varies horizontally and vertically as a result of atmospheric phenomena, maritime currents and tides. The distribution of water temperatures is generally heterogeneous. Southeasterly winds and the Northeasterly Mistral wind are important in the origins of circulated and mixed water masses. These winds are both frequent and strong. They have, as a result, a great effect on the water environment of Lake Etang de Berre. In theory, the annual precipitation in this region is well over eight times the water mass of the lake. The water of the Durance River flows into Lake Etang de Berre through the EDF Canal, amounting to 90% of the precipitation. However, reduction of rainfall in dry seasons has a serious effect on the hydrological characteristics of the lake. The temperature in the winter is partially caused by the low temperature of fresh water, particularly that of the Durance River. The hydrological season of fresh and brackish water is about one month ahead of the hydrological season of sea water in its vicinity. The salinity of Lake Etang de Berre runs approximately 3$\textperthousand$, except at lower levels and near the entrance to the Caronte Canal. However, when the volume of the Durance River water is reduced in the summer and fall, the salinity rises to 15$\textperthousand$. In the lake, the ratio of fresh water to sea water is six to one (6:1). The large quantities of seston conveyed by rivers, particularly the Durance diversion, strongly reduce the transparency in the brackish waters. Although the amount of sunshine is also notable, transparency is slight because of the large amount of seston, carried chiefly by Tripton in the fresh water of the Durance River. Therefore, photosynthesis generally occurs only in the surface layer. The transparency progressively increases from freshwater to open seawater, as mineral particles sink to the bottom (about 1.7kg $m^{-2}a^{-1}$ on the average in brackish lakes). The concentration of dissolved oxygen and the rate of oxygen saturation in seawater (Carry-le-Rouet) ranged from 5.0 to 6.0 $m\ell$ㆍ.$1^{-1}$, and from 95 to 105%, respectively. The amount of dissolved oxygen in Etang de Berre oscillated between 2.9 and 268.3%. The monographs of phosphate, nitrate, nitrite and silicate were published as a part of a study on the ecology of phytoplankton in these environments. Horizontal and vertical distributions of these nutriments were studied in detail. The recent diversion of the Durance River into Lake Etang de Berre has effected a fundamental change in this formerly marine environment, which has had a great impact in its plankton populations. A total of 182 taxa were identified, including 111 Bacillariophyceae, 44 Chlorophyceae, and 15 Cyanophyceae. The most abundant species are small freshwater algae, mainly Chlorophyceae. The average density is about $10^{8}$ cells $1^{-1}$ in Lake Etang de Berre, and about double that amount in Lake Etang de Vaine. Differences in phytoplankton abundance and composition at the various stations or at various depths are slight. Cell biovolume V (equivalent to true biomass), plasma volume VP (‘useful’ biomass) and, simultaneously. the cell surface area S and S/V ratio through the measurement of cell dimensions were computed as the parameters of phytoplankton productivity and metabolism. Pigment concentrations are generally very high on account of phytoplankton blooms by Cyanophyceae, Chlorophyceae and Cryptophyceae. On the other hand, in freshwaters and marine waters, pigment concentrations are comparatively low and stable, showing slight annual variation. The variations of ATP concentration were closely related to those of chlorophyll a and phytoplankton blooms only in marine waters. The carbon uptake rates ranged between 38 and 1091 mg$Cm^{-2}d^{-1}$, with an average surface value of 256 mg; water-column carbon-uptake rates ranged between 240 and 2310 mg$Cm^{-2}d^{-1}$, with an average of 810, representing 290 mg$Cm^{-2}$, per year 45 000 tons per year of photosynthetized carbon for the whole lake. Gross photosynthetic production measured by the method of Ryther was studied over a 2-year period. The values obtained from marine water(Carry-le-Rouet) ranged from 23 to 2 337 mg$Cm^{-2}d^{-1}$, with a weighted average of 319, representing about 110 gCm$^{-2}$ per year. The values in brakish water (Etang de Berre) ranged from 14 to 1778 mg$Cm^{-2}d^{-1}$, with a weighted average of 682, representing 250 mg$Cm^{-2}$ per year and 38 400 tons per year of photosynthesized carbon for the whole lake.

Development of Heat Exchanger for Fermentation Heat Utilization from Waste Woody Biomass (목질계 폐바이오메스의 발효열이용 열교환기의 개발)

  • Cho, Nam-Seok;Choi, Tae-Ho;Kim, Hong-Eun;Lee, Suk-Ho;Lee, Chung-Koo
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.1
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    • pp.94-104
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    • 2009
  • It is urgently required to develop the production of fermentation-heat energy from the waste agricultural and forest biomass and its effective heat exchanging system for the supply of warm water to rural households and greenhouses. In this study 3 helical-type and 1 plate-type heat exchangers using 3 different waste biomasses [e.g. hardwood (HW) sawdust (100%), softwood (SW) sawdust : HW sawdust (50 : 50) and HW sawdust : grass (90 : 10)] were applied in order to find out the best heat recovery system. The heat exchanger was basically considered to improve the overall heat recovery efficiency, to minimize heat loss and to simplify manufacturing, assembling and breaking up the fermenting beds. The helical-type heat exchanger (HX-H3) installed in fermenting bed of HW sawdust : grass (90 : 10) showed relatively higher temperature profiles, in particular mid- and upper-parts than lower and surface parts during 45-day fermentation process. The maximum temperature was ranged from $40^{\circ}C$ to $65^{\circ}C$ with average $60^{\circ}C$. The water temperature of tank outlet was ranged to $33{\sim}48^{\circ}C$ during whole measuring periods. By the way plate-type one (HX-P) installed in same biomass compositional fermenting bed showed $64.5{\sim}76.5^{\circ}C$ at center part, and $43{\sim}56^{\circ}C$ and $42{\sim}58^{\circ}C$, water tank and tank outlet temperatures, respectively, during 100 day measurement. It could be concluded that the plate-type heat exchanger (HX-P) provides not only the effective heating for the rural households and greenhouses, but also having the best heat recovery performance, easy manufacturing, assembling and breaking up the systems.

Growth and Fruiting Characteristics and No. of Acorns/tree Allometric Equations of Quercus acuta Thunb. in Wando Island, Korea (완도지역 붉가시나무의 성장 및 결실 특성과 종실량 상대성장식)

  • Kim, Sodam;Park, In-Hyeop
    • Korean Journal of Environment and Ecology
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    • v.33 no.4
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    • pp.440-446
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    • 2019
  • This study examined the growth and fruiting characteristics and the acorns biomass allometric equation of Quercus acuta to provide reference data related to the growth and seed supply during the restoration of evergreen forest in the warm temperate zone in Wando Island, Korea. For the growth survey, we selected and cut three sample trees having a mean diameter at breast height (DBH) to investigate the growth analysis through a stem analysis. We then developed the allometric equation (Y=aX+b) of DBH and tree height growth characteristic (Y) according to the average tree age (X) of sampled trees and estimated the DBH and tree height according to the age of Quercus acuta. For the fruiting survey, we selected and cut three sample trees with full fruit in August when, they are at the early mature fruiting stage, for the analysis. To develop the acorns/tree biomass allometric equation of Quercus acuta, we selected and cut ten sample trees of evenly divided diameters. The acorns biomass allometric equation ($Y=aX^b$) was derived by analyzing the biomass (Y) and the growth characteristics (X), such as the DBH, tree height, crown width, and crown height. The allometric equations of average tree age according to DBH and tree height were Y=0506X-2.064 ($R^2=0.999$) and Y=0.321X+0689 ($R^2=0.992$), respectively. The developed allometric equations estimated that the DBH were 3.0cm, 8.1cm, 13.1cm and 18.2cm while the tree heights were 3.9m, 7.1m, 10.3m, and 13.5m when the tree ages were 10, 20, 30, and 40 years, respectively. The analysis results of fruiting characteristics showed that the length, the diameter, the number of fruits, and the number of acorns per fruiting branch had the statistically significant difference and tended to decrease from the upper part to the lower part of crown downward. The total number of acorns was 1,312 acorns/tree in the upper part, 115 acorns/tree in the middle part, and 5 acorns/tree in the lower part of the crown. The allometric equation for the amount of acorns with DBH as an independent variable was $Y=0.003X^{4.260}$ with the coefficient of determination at 0.896. Although the coefficient of determination of the allometric equation using only DBH as the independent variable was lower than that using DBH and tree height ($D^2H$), it would be more practical to consider only DBH as the independent variable because of measurement errors.

A Study on the Growth Change by DBH Class in Korean White Pine Plantations (잣나무 인공림의 흉고직경 등급별 생장변화에 관한 연구)

  • Jung, Sunghoon;Seo, Yeongwan;Lee, Daesung;Choi, Jungkee
    • Journal of Korean Society of Forest Science
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    • v.111 no.1
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    • pp.125-135
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    • 2022
  • In this study, growth changes of the diameter at breast height (DBH), height, basal area, volume, and biomass of Korean white pine (Pinus koraiensis Siebold & Zucc.) on a plantation were examined via long-term monitoring. In addition, this study was performed to provide the basic data for timber production in line with DBH class by comparing the growth of the relative DBH size. Growth characteristics according to DBH class were analyzed by categorizing trees into five classes based on sorted DBH rankings: class I (1%-20%; upper 20%), class II (21%-40%), class III (41%-60%), class IV (61%-80%), class V (81%-100%; lower 20%). A total class (0%-100%) was also used. Total increment and mean annual increment (MAI) were calculated using data from nine measurements taken over 39 years. Tree characteristics based on average values and stand characteristics based on unit area per hectare were examined. According to the total increments of variables, the differences in DBH, basal area, volume, and biomass among classes I-V increased over time, whereas the height difference did not continually increase. According to MAI, the maximum DBH value was 0.92 cm·yr-1 at age 23 in class I, whereas the maximum value in all trees was 0.69 cm·yr-1 at age 17. The maximum value of height MAI for class I was 0.52 m·yr-1 at age 23, whereas that for all trees was 0.49 m·yr-1 at age 20. In terms of basal area, volume, and biomass growth at tree-and stand-level, the maximum MAI of class I and all trees was not observed during the measurement period. Therefore, additional long-term monitoring data are required to determine the maximum MAI of the variables.

Evaluation of Effective Sensing Distance and Measurement Efficiency for Ground-Based Remote Sensors with Different Leaf Distribution in Tobacco Plant (연초의 엽위 분포형태에 따른 지상 원격센서의 유효 탐사거리와 측정 효율성 평가)

  • Jeong, Hyun-Cheol;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.2
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    • pp.126-136
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    • 2008
  • Tobacco plants grown in pots by sand culture for 70 days after transplanting were used to evaluate the sensing distance and measurement efficiency of ground-based remote sensors. The leaf distribution of tobacco plant and sensing distance from the sensors to the target leaves were controlled by two removal methods of leaves, top-down and bottom-up removal. In the case of top-down removal, the canopy reflectance was measured by the sensor located at a fixed position having an optimum distance from the detector to the uppermost leaf of tobacco every time that the higher leaves were one at a time. The measurement of bottom-up removal, a the other hand, was conducted in the same manner as that of the top-down removal except that the lower leaves were removed one by one. Canopy reflectance measurements were made with hand held spectral sensors including the active sensors such as $GreenSeeker^{TM}$ red and green, $Crop\;Circle\;ACS-210^{TM}$ red and amber, the passive sensors of $Crop\:Circle^{TM}$, and spectroradiometer $SD2000^{TM}$. The reflectance indices by all sensors were generally affected by the upper canopy condition rather than lower canopy condition of tobacco regardless of sensor type, passive or active. The reflectance measurement by $GreenSeeker^{TM}$ was affected sensitively at measurement distance longer than 120 cm, the upper limit of effective sensing distance, beyond which measurement errors are appreciable. In case of the passive sensors that has no upper limit of effective distance and $Crop\;Circle^{TM}(ACS210)$ that has the upper limit of effective sensing distance specified with 213 cm, longer than that of estimated distance, the measurement efficiency affected by the sensing distance showed no difference. This result suggests that it is necessary to use the sensor specified optimum distance. The result revealed that active sensors are more superior than their passive counterparts in establishing between the relative ratio of reflectance index and the dry weight of tobacco treated by top-down removal, and in the evaluation of biomass. $The\;Crop\;Circle\;ACS-210^{TM}$ red was proved to have the highest efficiency of measurement, followed by $Crop\;Circle^{TM}(ACS210)$ amber and $GreenSeeker^{TM}$ red, $Crop\;Circle^{TM}$ passive, $GreenSeeker^{TM}$ green, and spectroradiometer, in descending order.