• Applied Biological Chemistry
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• v.38 no.3
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• pp.232-238
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• 1995

Quality changes of high fructose corn syrups (HFCS) were investigated during storage at $-5{\sim}60^{\circ}C$. Color properties such as spectrophotometric properties, HMF and Hunter color value increased continuously during storage period. And the change was most evident as storage temperature and fructose content were elevated. High fructose corn syrups all showed flow behaviors as dilatant fluid and their viscosities increased slightly during storage. Content of fructose decreased, while glucose, maltose and oligosaccharide contents increased during storage. Especially, crystallization of what was observed below $13^{\circ}C$ during storage.

• Journal of Applied Biological Chemistry
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• v.49 no.1
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• pp.24-27
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• 2006

Four ursane-type triterpenoids, 2${\alpha}$,3${\alpha}$,19${\alpha}$-trihydroxy-24-norurs-4(23),12-dien-28-oic acid (1), 4(R),23-epoxy-2${\alpha}$,3${\alpha}$,19${\alpha}$-trihydroxy-24-norurs-12-en-28-oic acid (2), myrianthic acid (3), and tormentic acid (4), and a phenolic compound, ethyl gallate (5), from an EtOAc-soluble extract of the stems of Rumex japonicus, were subjected to in vitro bioassays to evaluate advanced glycation end products (AGEs) and rat lens aldose reductase (RLAR) inhibitory activity. Compounds 1 and 5 exhibited a significant inhibitory activity on AGEs formation with $IC_{50}$ value of 87 ${\mu}M$ and on RLAR with $IC_{50}$ value of 14.3 ${\mu}M$, respectively. Ethyl gallate (5) was isolated for the first time from this plant.

• Applied Biological Chemistry
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• v.35 no.5
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• pp.389-394
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• 1992

Effect of dry-heat treatment $(130{\sim}220^{\circ}C)$ on properties of corn starch was investigated. No significant changes of surface color and swelling power were observed. Blue value, water-binding capacity, solubility and degree of hydrolysis by acid or glucoamylase were increased; however, intrinsic viscosity and crystallinity were decreased as heating temperature was raised. Noticeable changes occurred between $170{\sim}190^{\circ}C$.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.183-188
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• 2014

In order to increase the nutritional quality of soybean meal (SBM) as an animal feed, Bacillus subtilis TP6, a previously isolated strain from an Indonesian traditional fermented soybean food, Tempeh, was used as a starter organism for solid-state fermentation. In the pre-treated SBM with water content of 60% (v/w), B. subtilis TP6 was grown to a maximum viable cell number of $3.5{\times}10^9CFU/g$. Compared to control, crude protein in Bacillus fermented SBM was increased by 16%, while raffinose, stachyose, and trypsin inhibitors were reduced by 31, 37, and 90%, respectively. The Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that proteins in the fermented SBM were remarkably hydrolyzed into smaller molecular masses, resulting in a decrease in large sized proteins. Our data suggested that B. subtilis fermentation could increase the nutritive value of SBM through reduction of anti-nutritive factors and improvement of protein quality by hydrolysis of soy protein. In addition, B. subtilis TP6 produced a functional ingredient, poly-${\gamma}$-glutamic acid which has various health benefits.

• Biomaterials and Biomechanics in Bioengineering
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• v.4 no.1
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• pp.9-20
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• 2019

Living beings are formed of advanced biological and mechanical systems which exist for millions of years. It is known that various animals and insects right from small ants to huge whales have different weight carrying capacities, which is generally expressed as a ratio of their own bodyweights i.e., Strength to Bodyweight Ratio (SBR). The puzzle is that when a rhinoceros beetle (scientific name: Dynastinae) can carry 850 times its own bodyweight, why a man cannot accomplish the same feat. There are intrinsic biological and mechanical reasons related to their capacities, as per biomechanics. Yet, there are underlining principles of engineering and structural mechanics which tend to solve this puzzle. The paper attempts to give a plausible answer for this puzzle through structural mechanics and experimental modeling techniques. It is based on the fact that smaller an animal or creature, it has larger value of weight lifting by self-weight ratio. The simple example of steel prism model discussed in this paper, show that smaller the physical model size, larger is its SBR value. To normalize this, the basic length of the model need to be considered and when multiplied with SBR, a constant is arrived. Hence, the aim of the research presented is to derive this constant on a pan-living being spectrum through size/scaling effect.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.419-428
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• 2019

Phytases are enzymes that can hydrolyze phytate and its salts into inositol and phosphoric acid, and have been utilized to increase the availability of nutrients in animal feed and mitigate environmental pollution. However, the enzymes' low thermostability has limited their application during the feed palletization process. In this study, a combination of B-value calculation and protein surface engineering was applied to rationally evolve the heat stability of Escherichia coli phytase. After systematic alignment and mining for homologs of the original phytase from the histidine acid phosphatase family, the two models 1DKL and 1DKQ were chosen and used to identify the B-values and spatial distribution of key amino acid residues. Consequently, thirteen potential amino acid mutation sites were obtained and categorized into six domains to construct mutant libraries. After five rounds of iterative mutation screening, the thermophilic phytase mutant P56214 was finally yielded. Compared with the wild-type, the residual enzyme activity of the mutant increased from 20% to 75% after incubation at $90^{\circ}C$ for 5 min. Compared with traditional methods, the rational engineering approach used in this study reduces the screening workload and provides a reference for future applications of phytases as green catalysts.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.3
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• pp.99-114
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• 2021

We examined a current status of damage in biodiversity and its causing factors in Hasidong Anin coastal dune, Gangneung-si, Gangwon province which is designated as ecological and landscape conservation area. In this study, we found that ecosystem and biodiversity have been primarily damaged by anthropogenic factors such as the construction of surrounding area, military facilities illegally dumped garbage and the expansion of windbreak forest. These factors occur to damage the landscape, ecosystem and biodiversity etc. There is a significant lack of basic data needed for preservation and restoration due to the lack of prior research and value assessment. In order to establish solutions for preservation and restoration, it is critical to collect fundamental data and implement value assessments. Therefore, further studies such as ecosystem services assessment, increasing biodiversity, spatial analysis and monitoring of various items related to coastal dunes are needed.

• Fisheries and Aquatic Sciences
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• v.25 no.7
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• pp.357-379
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• 2022

Increased fisheries products have raised by-products that are discarded due to low economic value. In addition, marine by-products are still rich in protein and nutritional value that have biological activities and give benefits to human health. Meanwhile, there is raised pressure for sustainability practices in marine industries to reduce waste and minimize the detrimental effect on the environment. Thus, valorization by-products through bioactive peptide mining are crucial. This review focus on various ways to obtain bioactive peptides from marine by-products through protein hydrolysis, for instance chemical hydrolysis (acid and based), biochemical hydrolysis (autolysis and enzymatic hydrolysis), microbial fermentation, and subcritical water hydrolysis. Nevertheless, these processes have benefits and drawbacks which need to be considered. This review also addresses various biological activities that are favorable in pharmaceutical industries, including antioxidant, antihypertensive, anticancer, anti-obesity, and other beneficial bioactivities. In addition, some potential marine resources of Indonesia for the marine biopeptide from their by-product or undesired marine commodities would be addressed as well.

• Journal of Dairy Science and Biotechnology
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• v.40 no.1
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• pp.23-34
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• 2022

In this study, to determine the importance of lactic acid bacteria (LAB) in Kombucha fermentation, biological functions, such as organic acid production and anti-inflammatory and antibacterial activities, of Kombucha, with or without LAB inoculation, were evaluated. Lactobacillus paracasei DK215, Saccharomyces cerevisiae C3, and Acetobacter pasteurianus P2 were selected as the inoculants. Organic acids were measured every 3 days from the end of fermentation using HPLC; the organic acid content of LAB-inoculated Kombucha was relatively high. Samples with or without LAB inoculation showed high antibacterial activity against Escherichia coli. The MTT assay results indicated no significant difference in concentration difference and cell death. In the NO production test, compared with the uninoculated Kombucha sample, the LAB-inoculated Kombucha sample exhibited a value similar to that of the group without LPS treatment. The levels of cytokine (IL-1α, IL-6, TNF-α) production were significantly lower than those of the LPS(+) group, indicating the anti-inflammatory activity potential of the Kombucha sample. This improvement in the biological function of the LAB-inoculated Kombucha further verifies the value of LAB in the fermented food and beverage industry.

• Membrane and Water Treatment
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• v.14 no.3
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• pp.115-120
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• 2023

The design theory for nanoporous filtration membranes needs to be established. The present study shows that the performance and technical advancement of nanoporous filtration membranes are determined by the fundamental parameter I (in the unit Watt^1/2) which is formulated as a function of the shear strength of the liquid-pore wall interface, the radius of the filtration pore, the membrane thickness, and the bulk dynamic viscosity of the flowing liquid. This parameter determines the critical power loss on a single filtration pore for initiating the wall slippage, which is important for the flux of the membrane. It also relates the membrane permeability to the power cost by the filtration pore. It is shown that for biological cellular membranes its values are on the scale 1.0E-8Watt^1/2, for mono-layer graphene membranes its values are on the scale 1.0E-9Watt^1/2, and for nanoporous membranes made of silica, silicon nitride or silicon carbonized its values are on the scale 1.0E-5Watt^1/2. The scale of the value of this parameter directly measures the level of the performance of a nanoporous filtration membrane. The carbon nanotube membrane has the similar performance with biological cellular membranes, as it also has the value of I on the scale 1.0E-8Watt^1/2.