• Bulletin of the Korean Chemical Society
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• 제35권2호
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• pp.383-391
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• 2014

Biotin-S-S-Phosphine was designed and synthesized as a potential tool for a proteomic study of O-GlcNAcmodified proteins. This reagent features a disulfide linker between a triarylphosphine moiety, which allows selective conjugation to azide-containing proteins, and a biotin moiety that can allow easy isolation through its strong affinity toward avidin-coated solid beads. The disulfide linkage within this reagent can allow the easy release of the bound molecules of interest, which is difficult to achieve when a biotin:avidin pair is used alone, by reducing the disulfide bond of the reagent with DTT. Preliminary in vitro biological assays with azidelabeled and unlabeled cell lysates and a pure protein Nup62 showed that the Biotin-S-S-Phosphine reagent is highly reactive toward the free thiol groups of proteins. When a molecular tool with a disulfide linker is applied to the enrichment of the molecules of interest from other species, it is important to block the free-thiols of the sample using exhaustive alkylation prior to the Staudinger ligation reactions to restore the bioorthogonal nature of this reaction.

• Journal of Microbiology
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• 제44권2호
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• pp.171-176
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• 2006

A strictly aerobic, non-motile, ovoid-shaped Alphaproteobacteria, designated strain $JC2049^T$ was isolated from a tidal flat sediment sample. The results of 16S rRNA gene sequence analysis indicated that this isolate belonged to the genus Thalassobius, with a sequence similarity of 96.9-97.3% to other valid Thalassobius spp. The cells required 1-7% NaCl for growth (optimum 2%) and accumulated $poly-\beta-hydroxybutyrate$. Nitrite was reduced to nitrogen, but nitrate was not reduced to nitrite. No genetic potential for aerobic anoxygenic photosynthesis was detected. The primary isoprenoid quinone (Ubiquinone-10), predominant cellular fatty acids $(C_{18:1}{\omega}7c,\;11\;methyl\;C_{18:1}\omega7c\;and\;C_{16:0})$ and DNA G+C content (61 mol %) were all consistent with the assignment of this isolate to the genus Thalassobius. Several phenotypic characteristics clearly distinguished our isolate from other Thalassobius species. The degree of genomic relatedness between strain $JC2049^T$ and other Thalassobius species was in a range of 20-43 %. The polyphasic data presented in this study indicates that our isolate should be classified as a novel species within the genus Thalassobius. The name Thalassobius aestuarii sp. novo is therefore proposed for this isolate; the type strain is $JC2049^T(=IMSNU\;14011^T=KCTC\;12049^T=DSM\;15283^T)$.

• Applied Biological Chemistry
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• 제39권6호
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• pp.419-423
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• 1996

SOS chromotest 방법을 이용하여 천연물에 포함된 항변이원성 물질의 활성을 정량화 할 수 있는 항변이원 활성단위 측정법을 개발하였다. 이 방법에 의하여 시료의 항변이원 활성을 특정 화학적 변이원이 유도하는 변이원성이 표준측정 조건하에서 50% 억제되는 시료의 양으로 수치화하는 것이 가능하게 되었다. 확립된 방법으로 쌀의 품종별 항변이원 활성 및 기타 곡류의 항변이원 활성을 조사하였다. 그 결과 변이품종 쌀인 자도와 향도가 취반용으로 사용되는 추청보다 항변이원 활성이 높았고, 일반적으로 검정콩이나 팥과 같은 잡곡의 항변이원 활성이 현미보다 높았다.

• Applied Biological Chemistry
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• 제18권2호
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• pp.56-60
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• 1975

완두유묘의 자엽 및 뿌리조직중 탄소-14 방사능을 Liquid scintillation counter로 신속히 측정하는 새로운 방법에 관하여 실험하였다. 식물조직을 액체공기에 처리하여 분쇄한 다음 Gel phosphor에 헌탁시켜, Liquid scintillation counter로 측정하였다. 이시료에 다시 내부표준물질을 가한 다음 계측효율을 구하여 Apparent absolute activity를 얻었다. 이렇게 얻은 헌탁시료의 절대방사능은 자기 흡수현상 때문에, 따로 Flask combustion 방법으로 구한 절대방사능 보다는 낮았으나, 항상 비례하였다. 따라서 동일한 실험조건하에서 상술한 비율을 얻으면, 완두조직중 탄소-14의 점대방사능은, 비교적 간편한 헌탁측정(suspension counting) 만으로 가능하였다. 본방법은 완두 외에 다른 식물조직중 탄소-14 분석에도 쓰일 수 있다.

• Journal of Applied Biological Chemistry
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• 제42권4호
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• pp.175-179
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• 1999

Grape seed oil was extracted using different preparatory treatments as follows: (1) grinding, (2) grinding and roasting, (3) grinding and wet- roasting, (4) grinding, roasting, and wet-roasting, and (5) grinding, wet-roasting, and wet-roasting. The highest antioxidant activity was obtained from the sample with the method (2). Initial states of oxidation were similar except method (1) that showed more oxidized state, being P.O.V.8. Acid values were observed in the range from 1.42 to 1.89. The lowest acid value was found as 1.42 in method (1) and those of others were somewhat higher, indicating that heating process of roasting produced some free fatty acids. From the results of sensory evaluation, the best odor and taste were obtained from the methods (2) and (3). Repetitive procedure of wet-roasting, like method 5, caused some loss of flavor components and decrease in the sensory evaluation score. Addition of grape seed oil (method 2) to soybean and perilla oil at the level of 20% retained considerable antioxidant activities as much as 4.3 and 5 times, respectively, than 100% soybean or perilla oil stored for 12 weeks. When soybean or perilla oil was mixed with 20% grape seed oils, P.O.V. decreased to half of that of unmixed oils.

• Archives of Pharmacal Research
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• 제16권3호
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• pp.213-218
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• 1993

($\pm$)-Higenamine is known as a cardiotonic principle of aconite root (root of Aconitum spp., Ranunculaceae). A simple and sensitive detection method for higenamine was developed by using gas chromatography-mass spectrometry (GC/MS). The recovery of higenamine after extraction and concentration with XAD-2 resin column was around 95% from rat biological fluids such as bile, plasma and urine. The limits of detection of higenamine in these biological fluids were approximately 0.1 ng/ml each. It has well been suggested that tetrahydroisoquinolines possessing catechol moiety such as higenamine should be subjected to the catechol-O-methyl transferase (COMT) activity in vivo. We detected two major peaks of presumed metabolites of higenamine in the total ion chromatogram obtained from the rat urine sample after the oral adminstration of ($\pm$)-higenamine. The scan mass spectrum of one of the metabolties coincided with those obtained from coclaurine $(C_6$-O-methyl higenamine) and those of the other metabolite are suggestive of isococlaurine $(C_7$-O-methyl higenamine).

• Journal of Korean Biological Nursing Science
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• 제20권2호
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• pp.114-121
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• 2018

Purpose: The purpose of this study was to investigate the rate of human papilloma virus (HPV) vaccinations among 12-year-old daughters and to analyze factors influencing the mothers' decisions to vaccinate their daughters. Methods: A cross sectional descriptive study was conducted using a convenience sample of 139 mothers. Data was analyzed using SPSS WIN 23.0. Descriptive statistics and a logistic regression analysis were conducted. Results: The vaccination rate of HPV among children was 43.9%. Mothers showed a higher knowledge of the HPV vaccination than of cervical cancer and of human papilloma virus. In self-efficacy scores, the subscores of self-regulation efficacy were higher than self-confidence and task difficulty preference. The factors influencing the vaccination of daughters included the age of the daughters at the time of the vaccination, the provision of information on the HPV vaccine, knowledge of the HPV vaccination, and self-confidence. Conclusion: In order to increase the HPV vaccination rate, it is necessary to provide accurate scientific knowledge to mothers. A variety of intervention strategies should be developed to enhance the confidence of mothers so that mothers who want to provide the HPV vaccination to their daughters are able to follow through and provide the vaccination.

• 한국수자원학회논문집
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• 제38권1호
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• pp.49-57
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• 2005

본 연구에서는 홍천강, 미호천, 내성천을 평가 대상하천으로 선정하여, '하천의 생물서식처 복원을 위한 하천 자연도평가 : I. 평가방법의 제안'에 따라 하천자연도평가를 시행하였다. 하천자연도의 종합평가결과 홍천강은 2등급(평균 1.92), 미호천은 2등급(평균 1.43), 내성천은 2등급(평균 2.31)으로 평가되었다. 수질과 하천자연도평가의 상관관계를 비교ㆍ분석한 결과, 수질과 하천자연도 종합평가결과의 상관계수가 0.575로 상관성이 매우 작게 분석되어, 하천자연도평가가 수질평가와 다른 관점에서 하천의 생태계를 평가하는 지표로 활용이 가능한 것으로 분석되었다.는 지표로 활용이 가능한 것으로 분석되었다.

• Journal of Korean Biological Nursing Science
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• 제20권1호
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• pp.20-29
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• 2018

Purpose: This study aimed to determine the relationships between particulate matter risk perception, knowledge, and perceived barriers and health-promoting behaviors among college students. Methods: Data for this cross-sectional study were collected from September 1 to 30, 2017. The study sample consisted of 85 students from a university, Seoul. Students not living in the Seoul metropolitan area during the spring 2017 semester were excluded from participation. Pearson's correlation coefficient was used to identify relationships among study variables. Results: A significant positive correlation existed between particulate matter risk perception and health-promoting behaviors related to particulate matter (r= .51, p< .001). Among the risk perception subdomains, attention (r= .47, p< .001) and health effect (r= .55, p< .001) showed strong positive relationships with health-promoting behaviors. No significant relationships were found between knowledge (r= .12, p= .288) or perceived barriers (r= -.12, p= .264) and health-promoting behaviors related to particulate matter. Conclusion: Based on the study results, strategies for enhancing particulate matter risk perception are needed to increase the level of health-promoting behaviors related to particulate matter among college students.

• Applied Biological Chemistry
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• 제22권3호
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• pp.142-144
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• 1979

경기도(京畿道) 용인군(龍仁郡)과 화성군(華城郡)의 농촌지역(農村地域)에서 음료수(飮料水)를 취(取)하여 세균검사(細菌檢査)를 한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 총생균수는 3지역의 시료만 100/ml이하였으며 $1{\times}10^4/ml$ 이상은 6지역이었으며 최고 $1{\times}10^5/ml$로 나타났다. 2. Faecal Streptococcus는 용인군(龍仁郡)의 4개 지역의 시료에서 검출(檢出)되었다. 3. 음료(飮料) 적합여부(適合如否)를 국립보건 연구원의 표준비교에 의하여 판정한 결과 할 지역의 시료를 제외한 모두가 음료(飮料) 부적(不適)내지 음료적합여부(飮料適合如否)가 의심스러운 것으로 밝혀졌다.