• Korean Journal of Biological Psychiatry
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• v.20 no.2
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• pp.45-53
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• 2013

Objectives Non-major depression with fewer symptoms than required for a Diagnostic and Statistical Manual of Mental Disorders-4th edition diagnosis of major depressive disorder (MDD) has consistently been found to be associated with functional impairment. In this study, we aim to estimate the cognitive impairment and the quality of life in elderly patients with subsyndromal depression (SSD) compared with non-depressive elderly (NDE). Methods The Korean version of Mini International Neuropsychiatric Interview was administered to 194 outpatients with depression and 108 normal controls. SSD is defined as having five or more current depressive symptoms with core depressive symptoms (depressive mood or loss of interest or pleasure) during more than half a day and more than seven days over two weeks. Depression was evaluated by the Korean form of Geriatric Depression Scale of a 15-item short version. Global cognition was assessed by Mini-Mental State Examination in the Korean version of CERAD assessment packet (MMSE-KC). Subjective cognitive impairment was assessed by the Subjective Memory Complaint Questionnaire. Quality of life was evaluated by the Korean Version of Short-Form 36-Item Health Survey. Results The mean score of the MMSE-KC in the SSD group was lower than that in the NDE group with adjustment for age, gender, and education [F = 4.270, p = 0.04, analysis of covariance (ANCOVA)]. If we defined those having Z-score of MMSE-KC < -1.5 as a high risk group of cognitive impairment, the odds ratio for the high risk group of cognitive impairment was 1.86 [95% confidence intervals (CI) 1.04-3.34] in SSD and 7.57 (95% CI 3.50-16.40) in MDD compared to NDE. The scores of physical component summary (F = 9.274, p = 0.003, ANCOVA) and mental component summary (F = 53.166, p < 0.001, ANCOVA) in the SSD group were lower than those in the NDE group with adjustment for age, gender, and education. Conclusions The subjects with SSD, as well as those with MDD, showed impairment of global cognition and also experienced low quality of life in both physical and mental aspects, compared to the NDE group.

• Journal of Applied Biological Chemistry
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• v.60 no.4
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• pp.343-349
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• 2017

This study was conducted to investigate the residual level and the amount of transfer to lettuce grown in field condition treated with the 5-methyl-1,2,4-triazolo[3,4-b][1,3]benzothiazole (Azoxystrobin). The field trials on lettuce were carried out at two different green houses located in Gwangju (Field 1) and Yongin (Field 2). Soil and lettuce samples were collected at different days after soil treatment of azoxystrobin with two different concentrations, respectively. Average recoveries for azoxystrobin ranged from 86.9 to 113.6% from soil and lettuce with the variation coefficient of 0.1-4.6%. The initial concentrations of azoxystrobin in Gwangju soil were 9.20, 11.00 mg/kg and decreased to 1.36, 2.70 mg/kg at 43 DAT (days after treatment) in field 1, while 1.06, 2.23 mg/kg decreased to 0.20 and 0.67 mg/kg at 36 DAT in field 2, respectively. The half-lives of azoxystrobin were about 19.4 and 23.3 days for the low and high concentration of azoxystrobin treated soils in field 1 and 11.5 and 17.8 days in field 2 soils, respectively. Residue levels of azoxystrobin in lettuce were not detected in filed 1 and field 2 soils, respectively.

• Journal of the Korean Society of Child Welfare
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• no.58
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• pp.103-139
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• 2017

The objective of this study was to examine in depth the life of Korean-Chinese adolescents before and after entering Korean society. These adolescents experienced early separation from their biological mothers in China and were raised by relatives due to the absence of parents. The adolescents' childhoods were filled with a sense of loneliness and emptiness, which made them feel hopeless in life, and traveling to Korea in hope and fear was a major challenge in their lives. After coming to Korea, they harmed themselves by engaging in toxic behavior and found themselves hopeless. They also experienced difficulties due to the relationships that needed to be rebuilt with their biological parents, as well as the relationships with their stepparents and half-siblings. However, they reported feeling self-confident and hopeful that their life would improve in the future. In addition, family support and social-support systems have given them a potential avenue for development. Verbal data collected from seven Korean-Chinese adolescents were analyzed. Based on the results, practical recommendations to be reflected in related programs were provided.

• Korean Journal of Environmental Agriculture
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• v.39 no.4
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• pp.305-311
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• 2020

BACKGROUND: The healthy food trend has encouraged the consumption of natural products, including berries. This trend is expected to increase the strawberry consumption. There has been a concern about the exposure of pesticides approved for use on strawberry. In this study, the dissipation patterns of systemic and non-systemic pesticides were evaluated in strawberry under plastic-covered greenhouse conditions. METHODS AND RESULTS: Cyflumetofen and dimethomorph were applied on strawberry in the critical GAP (Good Agricultural Practices). Strawberries were harvested at 0, 1, 2, 3, 5, 7 and 10 days after final application of the pesticides. The analyses of the residual pesticides were performed by HPLC-DAD with C18 column. The limits of quantitation (LOQ) of cyflumetofen and dimethomorph were 0.04 and 0.02 mg/kg, respectively. The recovery of cyflumetofen and dimethomorph were 88.1 ~ 103.3% and 79.0 ~ 110.2% for the spiked two levels (LOQ and 10LOQ), respectively. The biological half-lives of cyflumetofen and dimethomorph werer 7.5 and 8.9 days, respectively. The dissipation rates in strawberry were calculated by the statistics method at a 95% confidence level. The distribution showed that pesticides with low log Pow were indicated by the decreased dissipation rate and pesticides with similar log Pow and low solubility also showed the decreased dissipation rate. CONCLUSION: The residues of cyflumetofen and dimethomorph in strawberry at time 0 after the final application were below the established MRL in Korea. The dissipation behavior of systemic and non-systemic pesticides in strawberry is affected by their log Pow and water solubility values.

• Korean Journal of Environmental Agriculture
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• v.42 no.1
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• pp.21-27
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• 2023

Pre-harvest residue limits (PHRLs) have been proposed as criteria for a proactive role to exceed the maximum residue limit (MRL) of pesticides in agricultural products at harvest. However, PHRL numbers are significantly less than those of established MRLs. This study was performed to determine the dissipation constants and residual concentrations of lufenuron after application on crown daisy and chamnamul under green house conditions. Two residue field trials for each crown daisy and chamnamul were selected to consider a different geographical site at least 20 km far from one another. The pesticide was treated according to critical GAP. After samples were sprayed with lufenuron, they were collected at 0, 1, 3, 5, 7, 10, and 14 days and analyzed using HPLC-DAD. The mean recoveries of crown daisy and chamnamul were within the range of 70-120% with below 20% coefficient variation, which is within the acceptable limits specified by the manual of pre-harvest residue study for pesticides (MFDS, 2014). The biological half-lives in field I and field II were 7.0 and 4.6 days for crown daisy and 2.7 and 2.8 days for chamnamul, respectively. The lower bounds of 95% confidence intervals of dissipation rate constants of lufenuron in crown daisy were determined to be 0.0692 and 0.1298 for field I and field II, respectively, and in chamnamul were 0.2067 for both field I and field II. After applying lufenuron 5% EC, the lufenuron residues on crown daisy and chamnamul at the pre-harvest intervals (14 days for crown daisy and 7 days for chamnamul) were below the safe levels. The dissipation rates of lufenuron in crown daisy and chamnamul were evaluated for similarities with leafy vegetables based on a 95% confidence interval.

• The Korean Journal of Pesticide Science
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• v.13 no.1
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• pp.13-20
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• 2009

Two insecticides, commonly used for Chinese cabbage, etofenprox and methoxyfenozide, were subjected to a field residue trial to evaluate safeties of the residues at harvest. The pesticides were sprayed onto the crop at recommended and double doses 10 days before the prearranged harvest and then sampling was done at 0, 1, 2, 3, 5, 7, 10, and 12 days after spraying. The amounts of pesticides residues in the crop were analyzed by chromatographic methods. Limits of detection (LODs) of both etofenprox and methoxyfenozide were $0.01mg\;kg^{-1}$ and mean recoveries were $96.76{\pm}2.67$ (CV=2.76%) and $95.84{\pm}2.57%$(CV=2.69%) in case of etofenprox and $103.26{\pm}3.21$ (CV=3.11%) and $94.50{\pm}1.35%$(CV=1.43%) in case of methoxyfenozide, respectively. Biological half-lives of etofenprox and methoxyfenozide were 3.2 and 3.5 days at the recommended dose and 2.7 and 3.5 days at the double dose, respectively. Initial residue levels of the pesticides at the recommended and double doses exceeded their MRLs, but final residue levels of the pesticides in the crop samples at harvest were less than their MRLs. The ratios of the EDI to ADI by intake the crop harvested 10 days after spraying were less than 4% of their ADIs, representing that residue levels of two pesticides at harvest were evaluated as safe.

• The Korean Journal of Pesticide Science
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• v.12 no.1
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• pp.67-73
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• 2008

The dissipation patterns of a boscalid in cucumber under greenhouse condition was investigated to establish pre-harvest residue limit (PHRL) and biological half-life. Initial concentration of boscalid in cucumber at standard application rate was $7.29\;mg\;kg^{-1}$ and decreased to $0.04\;mg\;kg^{-1}$ after 15 days with half-life of 1.9 day, while the initial concentration was $14.69\;mg\;kg^{-1}$ and decreased to $0.11\;mg\;kg^{-1}$ after same period with half lift of 2.0 day at double application rate. PHRL was suggested by prediction curve derived from the decay curve of boscalid at double rate treatment. For example, $10.39\;mg\;kg^{-1}$ was calculated for 10 days before harvest, and $1.73\;mg\;kg^{-1}$ for 5 days. Dilution effect was major factor far the decrease of boscalid residue due to fast increasement of weight of cucumber during cultivation. Final residues level of boscalid was predicted based on the dissipation curve and guideline on safe use, when boscalid was used to control powdery mildew and gray mold. At standard rate application, $1.26\;mg\;kg^{-1}$ and $1.33\;mg\;kg^{-1}$ were calculated as final residue levels for control powdery mildew and gray mold, respectively, which are above the MRL(Meximum Residue Limit).

• Applied Biological Chemistry
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• v.37 no.3
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• pp.182-188
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• 1994

Similar to the $^{42}K$ uptake, $^{86}Rb$ uptake by the roots of Hordeum distichum grown in the hydroponic culture was negatively correlated with the concentration of K supplied previously, showing that $^{86}Rb$ can be used for the K-bioassay. $^{86}Rb$ having longer half life (18.86 day) than $^{42}K$ (12.36 hr) allowed the use of larger number of root samples. $^{86}Rb$ uptake of 3 years old Citrus unshiu Marc. grown in water culture decreased drastically with the increase of K concentration of the culture solution, thus demonstrating that the nutrition status of K for citrus trees can be diagnosed by K-bioassay using $^{86}Rb$ tracer. $^{86}Rb$ uptake by the excised roots of Hordeum distichum grown in the pot with different K fertilizations was well correlated with the exchangeable K in soil. The amount of exchangeable K in soil for the optimal plant growth can be determined by its relationship. $^{42}K$ and $^{86}Rb-uptake$ by the Hordeum distichum roots were markedly inhibited by $5{\times}10^{-3}\; M$ KCN in the bioassay solution, indicating that uptake is energy-dependent. There was no significant relationship between K content in citrus leaves and K concentration in the water-culture medium. It is concluded that K-bioassay is a potentially useful tool for determining of K requirement in citrus trees.

• Journal of Pharmaceutical Investigation
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• v.9 no.3
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• pp.1-8
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• 1979

The main route of metabolism of isonicotinic acid hydrazid (INH) in man is its conjugation with acetyl coenzyme A to form acetyl-INH. The reaction is catalyzed by an N-acetyl transferase in the liver. The acetylated drug can be excreted by the kidney more efficiently than INH, and the biological half-life of the drug in the body depends upon how rapidly the drug can be acetylated. This report measured the concentration of INH in the blood of 147 individuals 6 hours after they received a standard dose (9.8mg/kg) and plotted the data as a frequeney distribution hiotogram. There was bimodality, with a mean for one subpopulation at approximately $0.6{\sim}0.8\;mcg/ml.$, and a mean for the other subpopulation between 2.8 and 4.0mcg/ml. As might be expected slow acetylators of INH are more likely to develop a cumulative toxicity to the drug. The principle ,toxicity to INH is a peripheral neuritis but this adverse effect can be prevented by given extra pyridoxin to the patients, and the vitamin does not alter the antitubercular activity of INH. This report carried out that pyridoxine does not alter the ratio of free INH to the total INH in blood.

• Journal of Microbiology and Biotechnology
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• v.17 no.6
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• pp.897-904
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• 2007

The aim of this paper is to discuss the methodology of our investigation of the dynamics of protein degradation and the total in situ protealytic activity in meso/eutrophic, eutrophic, and hypereutrophic freshwater environments. Analysis of the kinetics and rates of enzymatic release of amino acids in water samples preserved with sodium azide allows determination of the concentrations of labile proteins $(C_{LAB})$, and their half-life time $(T_{1/2})$. Moreover, it gives more realistic information on resultant activity in situ $(V_{T1/2})$ of ecto- and extracellular proteases that are responsible for the biological degradation of these compounds. Although the results provided by the proposed method are general y well correlated with those obtained by classical procedures, they better characterize the dynamics of protein degradation processes, especially in eutrophic or hypereutrophic lakes. In these environments, processes of protein decomposition occur mainly on the particles and depend primarily on a metabolic activity of seston-attached bacteria. The method was tested in three lakes. The different degree of eutrophication of these lakes was clearly demonstrated by the measured real proteolytic pattern and confirmed by conventional trophic state determinants.