• 한국군사과학기술학회지
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• 제14권6호
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• pp.1159-1165
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• 2011

Telemetry techniques of rats have been used for assessing safety pharmacology of drugs and chemicals. Biological signals including blood pressure and heart rate measured under anesthesia were significantly different from those obtained under normal conditions. The stress of restraint in awake animals can also affect the accuracy of physiological evaluation. This paper details the surgery required to allow key cardiovascular parameters to be determined. The telemetric measurement of cardiovascular parameters such as blood pressure, heart rate, electrocardiograph(ECG) established. We carried out the continuous monitoring of cardiovascular parameters using the telemetry system in F344 rats. During the measurement, no significant changes were observed in the heart rate and blood pressure. ECG signals and body temperature were also constant during the measurement of biological signals. With the results of this study, we conclude that this telemetry system can be applied usefully for the assesment of biological parameters in the rats.

• 한국미생물·생명공학회지
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• 제48권4호
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• pp.533-538
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• 2020

To investigate the effect of the predominant microorganisms in kimchi on quality, Leuconostoc mesenteroides ATCC 8293 was used as starter culture during kimchi fermentation. A higher number of lactic acid bacteria and lower initial pH were observed in starter kimchi than in non-starter kimchi in the early stage of fermentation. The concentrations of the main metabolite, lactic acid, were 69.88 mM and 83.85 mM for the non-starter and starter fermented kimchi, respectively. The free sugar concentrations of starter kimchi decreased earlier than those of non-starter kimchi, and the levels of free sugars in both kimchi samples decreased during fermentation. At the end of fermentation, non-starter kimchi had a softer texture than starter kimchi, suggesting that L. mesenteroides is useful in extending shelf life. Sensory evaluation showed that starter kimchi had higher sourness and lower bitterness and astringency values, resulting in high sensory quality. These results suggest that the L. mesenteroides ATCC 8293 strain could be a potential starter culture in kimchi.

• 한국버섯학회지
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• 제20권4호
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• pp.193-198
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• 2022

The biological efficiencies of promising Pleurotus spp. were evaluated. Pleurotus ostreatus, Pleurotus tuberregium,and Pleurotus sajor-cajuwere investigated at temperatures of 22℃, 20℃, and 18℃ to determine their ability to adapt to temperatures that are likely to be found in subtropical regions. The experiment was conducted using a substrate comprising Popular sawdust 60% + cotton seed 20% + beet pulp 20% under a relative humidity of 65%. The results indicated that there were significant differences in terms of biological efficiency between the species: P. tuber regium 128.84% at 22℃, P. ostreatus 108.41% at 22℃, and P. sajor-caju is 80% at 20℃. The common temperatures at which all species showed the highest biological efficiencies were 22℃ and 20℃. Therefore, the production of the evaluated species was equal to or surpassed biological efficiencies reported in tropical environments, thereby demonstrating a potential opportunity to adapt to tropical environments without compromising quality standards.

• 한국환경과학회지
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• 제27권6호
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• pp.383-399
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• 2018

In the present study, the community distribution, functional feeding groups, and habitat groups of the benthic macroinvertebrates from Gayasan National Park were investigated at eight different sites from 2015 to 2016. The correlation between habitat altitude and each group type was analyzed statistically. Biodiversity indices, including dominance, diversity, richness, and evenness indices, were calculated. The results showed a total of 4,216 individual benthic macroinvertebrates were collected, including 4 phyla, 6 classes, 16 orders, 40 families, and 100 species. Gammarus sobaegensis was dominant with 0.141% dominance, and the subdominant species was Epeorus nipponicus with 0.088% dominance. The EPT group(Ephemeroptera, Plecoptera and Tricoptera) accounted for 71.00% of the total species and 67.69% of the total individuals. The number of scraper species was highest, and the number of gathering collector individuals was highest among functional feeding groups. The individual number of scrapers(r=-0.358, p < 0.05) and gathering collectors(r=-0.343, p < 0.05) decreased with increasing altitude. The numbers of species and individuals of clingers and sprawlers were highest among the habitat groups. The individual number of clingers(r=-0.547, p < 0.01) and burrowers(r=-0.331, p < 0.05) significantly decreased with increasing altitude. The diversity and richness indices were higher in St. 3 than in the other sites. The dominance index was highest in St. 2, and the evenness index was highest in St. 7. We evaluated the biological water quality with several known methods, and concluded that ESB(Ecological score of benthic macrovertebrates community) and GPI(Group polution index) were more suitable than KSI(Korean saprobic index) and BMI (Benthic macrovertebrate index) to assess the biological water quality of Gayasan mountain stream water.

• 한국잡초학회지
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• 제31권1호
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• pp.103-111
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• 2011

물달개비는 한국의 논에서 문제되는 난방제 잡초중의 하나이다. 물달개비의 생물학적 방제 인자로 물달개비바구미를 선발하였다. 생물학적 방제에 이용하기 위해 2006년과 2007년 계대사육을 실시하여 형태적, 생태적 특징을 조사하였다. 이 종은 1년에 한세대만 경과하며 성충으로 월동하고, 성충은 6월 하순부터 9월 하순까지 발생한다. 물달개비바구미는 알에서 성충까지 $22{\pm}0.7$일이 소요된다. 주요 농작물과 수생식물 60종을 대상으로 기주특이성을 조사 결과 물달개비의 생물학적 방제 곤충으로서의 가능성을 보였다.

• Applied Biological Chemistry
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• 제48권4호
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• pp.331-334
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• 2005

본 연구는 일반적으로 사용되는 DNA 추출방법들에 대한 효율을 비교하기 위해 수행하였다. 원료 옥수수 및 이를 가공한 시료들로부터 DNA추출을 하였으며, 추출된 DNA의 형상, 농도 및 순도 측정 그리고 PCR 분석 결과를 비교하였다. 5가지 방법으로 옥수수의 DNA를 추출한 결과, 추출방법에 따른 DNA 형상의 차이가 거의 없는 것을 확인하였으나, 각각의 시료들로부터 추출된 DNA의 양은 시료 g당 $0.25{\mu}g$부터 $234.0{\mu}g$까지 매우 다양하게 나타났다. 5가지 방법으로 옥수수 시료들의 DNA를 추출한 결과, CTAB법과 DNeasy plant Maxi 키트를 이용한 DNA 추출방법이 높은 수율을 보였다.

• Journal of Applied Biological Chemistry
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• 제56권4호
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• pp.219-227
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• 2013

This study was to investigate serum triglyceride (TG), phospholipid (PL), and cholesterol concentrations after feeding the combined diets of prepared traditional Cheonggukjang (TC) or Cheonggukjang made from sword bean powder (CS), the feed, and high-fat cholesterols at certain ratio for 8 weeks in rats, and then confirmed the effects of the diets based on the changes in the tissues of livers, kidneys, and spleens. The serum TG concentrations were lower in CS group compared to that of in TC group. The increase of PL concentrations was greater in CS group than that of TC group. FChCS6 group (92% feed+2% cholesterol+6% CS) showed the lowest total-cholesterol (T-C), $60.1{\pm}8.5mg/dL$, indicating that CS was effective to lower the T-C. Both TG/PL and T-C/PL ratio tended to be decreased. As CS intake was effective in the reduction of low density lipoprotein-cholesterol level as well as the increase of high density lipoprotein-cholesterol level, it would be effective in the prevention of adult diseases and circulatory diseases. It was confirmed that there were no changes in organ appearance and tissues in the rats with CS intake. Therefore, CS intake showed superior functionalities than that of TC intake.

• 한국수정란이식학회지
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• 제25권2호
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• pp.111-116
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• 2010

Sperm chromatin integrity is essential for successful fertilization and development of an embryo. Reported here is a quantification of DNA fragments which is intimately associated with reproductive potential to provide one of criteria for sperm chromatin integrity. Three sperm populations were considered: CONTROL (no treatment), UV irradiation (48mW/$cm^2$, 1h) and $H_2O_2$ (oxidative stress induced by hydrogen peroxide, 10 mM, 50 mM and 100 mM). DNA fragments in boar sperm were evaluated by using ligation-mediated quantitative real-time polymerase chain reaction (LM-qPCR) assay, which relies on real-time qPCR to provide a measure of blunt 5' phosphorylated double strand breaks in genomic DNA. The results in agarose gel electrophoresis showed no significant DNA fragmentation and no dose-dependent response to $H_2O_2$. However, the remarkable difference in shape and position was observed in melting curve of LM-qPCR. This result supported that the melting curve analysis of LM-qPCR presented here, could be more sensitive and accurate than previous DNA fragmentation assay method.

• 한국토양비료학회지
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• 제47권3호
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• pp.213-216
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• 2014

Arsenic is a known hazardous metalloid not only to the animals but also to plants. With high concentrations, it can impede normal plant growth and cause even death of plants at extremely high levels. A known plant response to stress conditions such as toxic levels of metal (loids) is the production of stress ethylene, causing inhibitory effect on root growth in plants. When the effect of various arsenic concentrations was tested to maize plant, the stress ethylene emission proportionately increased with increasing concentration of As(V). The inoculation of two arsenic tolerant bacteria; Pseudomonas grimonti JS126 and Pseudomonas taiwanensis JS238 having respective high and low 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity reduced stress ethylene emission by 59% and 30% in maize grown in arsenic polluted soils. The result suggested the possible use of Pseudomonas grimonti JS126 for phytoremediation of arsenic polluted soils.

• Journal of Korean Biological Nursing Science
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• 제25권3호
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• pp.183-204
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• 2023

Purpose: The purpose of this study was to evaluate the applicability of ChatGPT in biological nursing science education. Methods: This study was conducted by entering questions about the field of biological nursing science into ChatGPT versions GPT-3.5 and GPT-4 and evaluating the answers. Three questions each related to microbiology and pharmacology were entered, and the generated content was analyzed to determine its applicability to the field of biological nursing science. The questions were of a level that could be presented to nursing students as written test questions. Results: The answers generated in English had 100.0% accuracy in both GPT-3.5 and GPT-4. For the sentences generated in Korean, the accuracy rate of GPT-3.5 was 62.7%, and that of GPT-4 was 100.0%. The total number of Korean sentences in GPT-3.5 was 51, while the total number of Korean sentences in GPT-4 was 68. Likewise, the total number of English sentences in GPT-3.5 was 70, while the total number of English sentences in GPT-4 was 75. This showed that even for the same Korean or English question, GPT-4 tended to be more detailed than GPT-3.5. Conclusion: This study confirmed the advantages of ChatGPT as a tool to improve understanding of various complex concepts in the field of biological nursing science. However, as the answers were based on data collected up to 2021, a guideline reflecting the most up-to-date information is needed. Further research is needed to develop a reliable and valid scale to evaluate ChatGPT's responses.