Methanol extracts of fresh materials of 57 plants were screened for in vivo antifungal activity against Magna-porthe grisea, Corticium sasaki, Botrytis cinerea, Phyto-phthora infestans, Puccinia recondita, and Blumeria graminis f. sp. hordei. Among them, seven plant extracts showed disease-control efficacy of more than 90% against at least one of six plant diseases. None of the plant extracts was highly active against tomato gray mold. The methanol extracts of Chloranthus japonicus (roots) (CjR) and Paulownia coreana (stems) (PcS) displayed the highest antifungal activity; the CjR extract controlled the development of rice blast, rice sheath blight, and wheat leaf rust more than 90%, and tomato gray mold and tomato late blight more than 80%. The PcS extract displayed control values of more than 90 % against rice blast, wheat leaf rust, and barley powdery mildew and more than 80% against tomato gray mold. The extract of PcS also had a curative activity against rice sheath blight and that of CjR had a little curative activity against rice blast. On the other hand, the extract of Rumex acetocella roots reduced specifically the development of barley powdery mildew. Further studies on the characterization of antifungal substances in antifungal plant extracts are underway and their disease-control efficacy should be examined under greenhouse and field conditions.
Kim, Byung-Ryun;Hahm, Soo-Sang;Han, Kwang-Seop;Kim, Jong-Tae;Park, In-Hee
한국균학회소식:학술대회논문집
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2016.05a
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pp.25-25
/
2016
Biological control has many advantages as a disease control method, particularly when compared with pesticides. One of the most important benefits is that biological control is an environmental friendly method and does not introduce pollutants into the environment. Another great advantage of this method is its selectivity. Selectivity is the important factor regarding the balance of agricultural ecosystems because a great damage to non target species can lead to the restriction of natural enemies' populations. The objective of this research was to evaluate the effects of several different bacterial isolates on the efficacy of biological control of soil borne diseases. White rot caused by Sclerotium cepivorum was reported to be severe disease of garlic and chive. The antifungal bacteria Burkholderia pyrrocinia CAB08106-4 was tested in field bioassays for its ability to suppress white rot disease. In field tests, B. pyrrocinia CAB08106-4 isolates suppressed white rot in garlic and chive, with the average control efficacies of 69.6% and 58.9%, respectively. In addition, when a culture filtrate of B. pyrrocinia CAB08106-4 was sprayed onto wounded garlic bulbs after inoculation with a Penicillium hirstum spore suspension in a cold storage room ($-2^{\circ}C$), blue mold disease on garlic bulbs was suppressed, with a control efficacy of 79.2%. These results suggested that B. pyrrocinia CAB08106-4 isolates could be used as effective biological control agents against both soil-borne and post-harvest diseases of Liliaceae. Chinese cabbage clubroot caused by Plasmodiophora brassicae was found to be highly virulent in Chinese cabbage, turnips, and cabbage. In this study, the endophytic bacterium Flavobacterium hercynium EPB-C313, which was isolated from Chinese cabbage tissues, was investigated for its antimicrobial activity by inactivating resting spores and its control effects on clubroot disease using bioassays. The bacterial cells, culture solutions, and culture filtrates of F. hercynium EPB-C313 inactivated the resting spores of P. brassicae, with the control efficacies of 90.4%, 36.8%, and 26.0%, respectively. Complex treatments greatly enhanced the control efficacy by 63.7% in a field of 50% diseased plants by incorporating pellets containing organic matter and F. hercynium EPB-C313 in soil, drenching seedlings with a culture solution of F. hercynium EPB-C313, and drenching soil for 10 days after planting. Soft rot caused by Pectobacterium carotovorum subsp. carotovorum was reported to be severe disease to Chinese cabbage in spring seasons. The antifungal bacterium, Bacillus sp. CAB12243-2 suppresses the soft rot disease on Chinese cabbage with 73.0% control efficacy in greenhouse assay. This isolate will increase the utilization of rhizobacteria species as biocontrol agents against soft rot disease of vegetable crops. Sclerotinia rot caused by Sclerotinia sclerotiorum has been reported on lettuce during winter. An antifungal isolate of Pseudomonas corrugata CAB07024-3 was tested in field bioassays for its ability to suppress scleritinia rot. This antagonistic microorganism showed four-year average effects of 63.1% of the control in the same field. Furthermore, P. corrugata CAB07024-3 has a wide antifungal spectrum against plant pathogens, including Sclerotinia sclerotiorum, Sclerotium cepivorum, Botrytis cinerea, Colletotrichum gloeosporioides, Phytophotra capsici, and Pythium myriotylum.
Purpose: The purpose of this study was to analyze the effects of a quit smoking program using the Web and short message service on exhaled carbon monoxide, self-efficacy, and depression according to nicotine dependency level in undergraduate students. Methods: In this study a non-equivalent control group pretest-posttest design was applied. The participants included 90 students (52 in the low nicotine dependency group and 38 in the high nicotine dependency group) who succeeded in quitting smoking. Data were collected on 3 occasions, that is, before the program, immediately after the program, and 3 weeks after the program. Collected data were analyzed using independent t-test, repeated measure ANOVA, and paired t-test with SPSS 20.0. Results: Exhaled carbon monoxide was higher in the high nicotine dependency group than in the low nicotine dependency group. Self-efficacy significantly increased 3 weeks after the program in the low nicotine dependency group and significantly increased immediately after the program in the high nicotine dependency group. Depression significantly decreased 3 weeks after the program in the low nicotine dependency group. Conclusion: Self-efficacy may be enhanced when it is dealt with during an early phase of the quit smoking program for the high nicotine dependency group. Long-term intervention and persistent intervention are needed with regard to depression during a quit smoking program.
Purpose: The purpose of this study was to describe sports injuries among elderly people and to compare physical activity, perceived health status and exercise self-efficacy between elderly people who had experienced a sports injury and elderly people who hadn't. Methods: The sample of this study were 100 elderly in Korea, 51 of whom had had a sports injury. The study used questionnaires to gather data. There were 8 demographic factors, 4 factors related to physical activity, perceived health status and exercise self-efficacy of the subjects. Data were analyzed by frequencies, chi-square test, and t-test. Result: Among the sports injured elders, one occurrence of a sport injury was 86.3%. There were more outdoor than indoor sports injuries of elders (66.7%), and sports injuries of elders occurred more in the winter (82.7%). The major cause of the sports injuries was loss of balance. Ankles were frequently damaged from these injuries. The most significant change after the sports injury was reduction of physical activity (60.8%). There were significant differences between sports injured elderly and non-injured elderly for age (${\chi}^2=2.58$, p=.011) and the amount of physical activity (t=1.96, p=.050), but there was no significant difference in perceived health status and exercise self-efficacy. Conclusion: To prevent sports injuries in the elderly means to maintain a medium level of physical activity and to improve their balance.
Purpose: The purpose of this study was to identify influencing factors on rehabilitation adherence in stroke patients. Methods: This study was a descriptive survey. A structured questionnaire was used for face-to-face interviews with a convenient sample of 192 subjects, who were admitted in 5 rehabilitation hospitals located in G metropolitan city. Results: The score of rehabilitation motivation in the subjects was a mean of $2.04{\pm}0.35$, self-efficacy $6.22{\pm}2.32$, family support $3.40{\pm}0.82$ and rehabilitation adherence $3.08{\pm}0.41$. The rehabilitation adherence was a statistically significant difference according to the education level (F= 3.40, p= .035), marital status (F= 4.04, p= .019), number of personal insurance policies (K= 9.80, p= .020), location of paresis (F= 2.72, p= .046), and status of current smoking (M = 657.00, p= .001). There was significant correlation among degree of rehabilitation adherence, rehabilitation motivation (r= .30, p< .001), self-efficacy (r= .14, p= .046) and family support (r= .18, p= .011). Rehabilitation motivation (${\beta}=0.19$, p= .007), self-efficacy (${\beta}=0.14$, p= .035), marital status (${\beta}=0.14$, p= .038), number of personal insurance policies (${\beta}=-0.15$, p= .045) and location of paresis(${\beta}=-0.15$, p= .028) were identified as significant predictors. This model explained 22.6% of variance in rehabilitation adherence (F= 5.92, p< .001). Conclusion: There is a need to develop an effective intervention for rehabilitation adherence improvement considering the identified variables in this study.
Purpose: This study was performed to identify the effects of dance sports programs on the parameters of health promotion (blood pressure, pulse, glucose, blood lipids, musculoskeletal pain, fatigue, self-efficacy, and depression) in rural women. Methods: A non-equivalent control group pretest-posttest experimental design was used. The study subjects were 38 women who were aged 45-78 and cultivating perilla leaves in a rural area. An experimental group (n=20) received the dance sports program from December 3rd, 2009 to June 3rd, 2010 and a control group (n=18) did not. Data was collected 3 times (before the program, 12 during and 24 weeks after the program) and were analyzed with $X^2$ test, t-test, and Repeated Measures ANOVA using SPSS/WIN 18.0. Results: For the experimental group, significant improvement was found in high density lipoprotein cholesterol (HDL-C), low density cholesterol (LDL-C), triglyceride (TG) musculoskeletal pain, fatigue, self-efficacy, and depression as compared to the control group. Conclusion: The findings of the study indicates that the dance sports program is effective on health promotion for rural women. Therefore, dance sports programs can be recommended for health promotion of rural residents.
Purpose: The purpose of this study was to develop a clinical practice nursing students' microbiology program based on the mastery learning model, and to evaluate the effects of the program on nursing students' knowledge, self-efficacy, performance, and satisfaction related to the nursing students' microbiology program. Methods: The program was developed by using the processes of the mastery learning model. The pre-experimental research design involved a one group pretest-posttest design. The setting was a university located in Incheon, Korea. A total of 130 nursing students participated in the program including a theoretical lecture, clinical practice, and formative and summative evaluation. Results: Using the program that was designed and developed, results for the total score of self-efficacy, knowledge, and performance in the post-test application were significantly higher than in the pre-test application (p<.05). The satisfaction of hand hygiene and disinfection/contaminated hand microbial culture and disinfection test received the highest ratings. Conclusion: The application of a clinical practice nursing students' microbiology program was effective, and can be expanded to other nursing students. Future research with other study designs was warranted in order to prove the effect of a microbiology program based on the mastery learning model.
Muthuramalingam, Karthika;Kim, Young Mee;Cho, Moonjae
Journal of Applied Biological Chemistry
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v.64
no.1
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pp.1-4
/
2021
Ambient air pollution, in particular, particulate matter (PM) pollution imposes serious health concerns such as hospitalization and premature deaths, worldwide. While commercial breathing masks are in use for protection against this hazardous issue, yet their efficiency in filtering PM was not up to the par, besides several other discomforts such as poor breathability due to reduced air flow, sweat production etc. In this study, commercial face mask coated with β-glucan, a high molecular weight polymer is tested for its efficacy in filtering PM. Quantification of PM before and after filtration and microscopic observation (using scanning electron microscopy (SEM)) of the fabric used in filtering the dust pollutants (generated from wood chips and cigarette) showed that β-glucan coated fabric were significantly efficient in capturing PM (size of 10 and 2.5 ㎛ in diameter) than that of the untreated control fabric, wherein the former had filtration efficacy with fold increase of 11.6 and 2.6 towards capturing PM2.5 and PM10 respectively than the latter. Thus, β-glucan coated fabric was found to be effective in filtering PM.
Purpose: This study aimed to investigate the status and factors associated with self-management behavior (SMB) of patients with type 2 diabetes (T2DM) in a border area of southwest China. Methods: A cross-sectional study was conducted with T2DM patients in Dali, China. The participants filled in the questionnaires including demographic and disease-related characteristics, psychosocial status, resources of DM knowledge, knowledge of DM care, competency in DM care skills, the Chinese Diabetes Management Self-Efficacy Scale (C-DMSES), and the Chinese Summary of Diabetes Self-Care Activities (C-SDSCA). All the data were analyzed with SPSS version 26. Multiple linear regression analysis examined associations between predictors and SMB. Results: A total of 470 valid questionnaires have been collected. The score for overall SMB was 50.71± 11.99; 19.6% of patients were at a good level, 48.3% were moderate, and 32.1% were poor. The significant factors that influenced SMB included self-efficacy (β= 0.37; p< .001), competency in DM care skills (β= 0.22; p< .001) and lacked in the treatment confidence (β= -0.09; p= .023). Conclusion: The level of self-management among T2DM patients in this area was medium-low state. For future studies, our findings suggest that self-efficacy, competency in DM care skills, and treatment confidence should be considered essential factors in improving the self-management behavior of T2DM patients in the border area of southwest China.
Park, Soon Yong;Jeong, Ae-Jin;Kim, Geun-Young;Jo, Ara;Lee, Joo Eon;Leem, Sun-Hee;Yoon, Joung-Hahn;Ye, Sang Kyu;Chung, Jin Woong
Journal of Microbiology and Biotechnology
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v.27
no.10
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pp.1877-1884
/
2017
Mesenchymal stem cells (MSCs) have been suggested as a primary candidate for cell therapy applications because they have self-renewal and differentiation capabilities. Although they can be expanded in ex vivo system, clinical application of these cells is still limited because they survive poorly and undergo senescence or apoptosis when transplanted and exposed to environmental factors such as oxidative stress. Thus, reducing oxidative stress is expected to improve the efficacy of MSC therapy. The milk protein lactoferrin is a multifunctional iron-binding glycoprotein that plays various roles, including reduction of oxidative stress. Thus, we explored the effect of lactoferrin on oxidative stress-induced senescence and apoptosis of human MSCs (hMSCs). Measurement of reactive oxygen species (ROS) revealed that lactoferrin inhibited the production of hydrogen peroxide-induced intracellular ROS, suggesting lactoferrin as a good candidate as an antioxidant in hMSCs. Pretreatment of lactoferrin suppressed hydrogen peroxide-induced senescence of hMSCs. In addition, lactoferrin reduced hydrogen peroxide-induced apoptosis via inhibition of caspase-3 and Akt activation. These results demonstrate that lactoferrin can be a promising factor to protect hMSCs from oxidative stress-induced senescence and apoptosis, thus increasing the efficacy of MSC therapy.
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