• Particle and aerosol research
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• v.13 no.1
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• pp.25-31
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• 2017

This paper offer the characteristics for the detection and classification of biological and non-biological aerosol particles in the air by using laser-induced-fluorescence (LIF) based Bio-aerosol Detection System (BDS). The BDS is mainly consist of an optical chamber, in-outlet nozzle system, 405 nm diode laser, an avalanche photo detector (APD) for scattering signal and photomultiplier tubes (PMT) for fluorescence signals in two different wavelength range ; F1, 510-600 nm and F2, 435-470 nm. The detection characteristics, especially ratio of fluorescence signal intensity were examined using well-known components : polystylene latex (PSL), fluorescence PSL, $2{\mu}m$ of SiO2 micro sphere, dried yeast, NADH, ovalbumin, fungicide powder and standard dust. The results indicated that the 405 nm diode laser-based LIF instrument can be a useful bio-aerosol detection system for unexpected biological threaten alter in real-time to apply for dual-use technology in military and civilian fields.

• Microbiology and Biotechnology Letters
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• v.31 no.3
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• pp.307-310
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• 2003

A gene encoding streptodornase(sdc) from Streptococcus equisimilis H46A was expressed in S. equisimilis H64H sdc under the control of the streptokinase gene promoter. Secretion of the streptodornase was directed by the signal sequences of streptokinase or streptodornase. The expressed streptodornase activity from S. equisimilis H46A sdc transformant with streptokinase promoter - streptodornase coding sequence fusion vector was 2.3 fold higher than that from wild type. Construct of signal sequence region replaced by streptokinase ones was similarly expressed as a wild type. But constructs of skc or lrp core regions of streptokinase promoter streptodornase fusion were similarly expressed as in sdc mutant. In conclusion, improved expression of streptodornase by use of streptokinase promoter required the full length of promoter.

• Journal of Biomedical Engineering Research
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• v.15 no.3
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• pp.305-316
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• 1994

All physical data in the real world are nonstationary signals that have the time varying statistical characteristics. Although few algorithms suitable to process the nonstationary signals have ever been suggested, these are treated the nonstationary signals under the assumption that the nonstationary signal is a piece-wise stationary signal. Recently, statistical analysis algorithms for the nonstationary signal have concentrated so much interest. In this paper, nonstationary EMG signals are mapped onto the orthogonal wavelet transform domain so that the eigenvalue spread of its autocorrelation matrix could be more smaller than that in the time domain. Then the model in the wavelet transform domain and an algorithm to estimate the model parameters are suggested. Also, an test signal generated by a white gaussian noise and the EMG signal are identified, and the algorithm performance is considered in the sense of the mean square error and the evaluation parameters.

• Journal of Biomedical Engineering Research
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• v.16 no.3
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• pp.301-308
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• 1995

A high-precision pre-amplifier is designed for general use in EEG measurement system. It consists of signal generator, signal amplifier with a impedance converter, shield driver, body driver, differential amplifier, and isolation amplifier. The combination of minimum use of inaccurate passive components and the appropriate matching of each monolithic amplifiers results in good noise behavior, low leakage current, high CMRR, high input impedance, and high IMRR. The performance of EEG pre-amplifier has been verified by showing the typical EEG pattevn of a nomad person through the clinical experiments.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.05
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• pp.79-81
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• 1994

Petubation in the fundamental frequency and in the peak amplitude of the EGG signal derived with a four-electrode EGG system were investigated for the purpose of developing useful measures for the detection of layngeal pathology. The data were compared to the degree of amplitude perturbation and frequency perturbation. There was a close relation between amplitude perturbation and frequency perturbation analysis of EGG signal and degree of laryngeal pathology.

• Journal of Biomedical Engineering Research
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• v.10 no.3
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• pp.285-288
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• 1989

A characteristic proton NMR signal is found in human cancer serums. The signal is given by $6.43{\pm}0.06$ ppm on average. An attempt is made to find a certain correlation between the appearance of the signal and the amounts of CEA and AFP in the serums.

• Journal of Biomedical Engineering Research
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• v.5 no.1
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• pp.5-8
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• 1984

As the ensemble averaged dz/dt signal during exercise gets smoothed, it is difficult to find the distinctive marks for estimation of stroke volume. The cross correlation function was made use of estmating these marks for automatic calculation by computer from the ensemble averaged dz/dt signal. LVET(Left Ventricular Ejection Time) and stroke volume were estimated based on the calculated parameters from the characteristic points. LVET, stroke volume calculated by hand, by the ensemble average and the cross correlation were compared for accuracy validation.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.11
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• pp.175-177
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• 1996

본 논문에서는 일반 심전도 모니터링 장비가 갖는 이동성 및 상시성의 제한을 극복하여 공중무선망을 이용한 심전도 모니터링 장치에 관한것으로서 특히, 활동중인 사람의 심전도 데이터를 공중 무선 통신망에서 제공하는 망써비스를 이용하여 원거리 모니터링 센터에서 모니터링 및 응급조치를 하는 시스템에 관한 것이다.

• Korean Journal of Environmental Agriculture
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• v.30 no.4
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• pp.390-394
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• 2011

BACKGROUND: Plant expression system for mass production of recombinant proteins has several advantages over other existing expression systems with economical and safety issues. Breast cancer is a cancer originating from breast tissue and comprises almost 25% of all cancers in women world widely. Lewis-Y antigen is difucosylated oligosaccharide and is carried by glycoconjugates at cancer cell surface. In this study, the anti-breast cancer mAb BR55, which recognizes the epitope Lewis-Y, was expressed in the plant expression system. METHODS AND RESULTS: We have developed plant system for production of mAb BR55 with or without KDEL (the ER retention signal). This ER retention signal was attached to C-terminus of protein to help retain the recombinant glycoprotein carrying oligomannose glycans and enhance glycoprotein accumulation. PCR analysis was performed and confirmed the presence of recombinant genes. Western blot validated that the recombinant proteins mAb BR55 with or without KDEL were expressed in transgenic plants, moreover, the expression level of the mAb BR55 with KDEL was higher compared to the mAb BR55 without KDEL. CONCLUSION: These results indicate that KDEL fusion is a good way to produce proteins and plant can be an ideal expression system to obtain proteins and enhance accumulation of proteins.

• BMB Reports
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• v.53 no.12
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• pp.640-645
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• 2020

Suppressors of cytokine signaling (SOCS) exhibit diverse anti-inflammatory effects. Since ROS acts as a critical mediator of inflammation, we have investigated the anti-inflammatory mechanisms of SOCS via ROS regulation in monocytic/macrophagic cells. Using PMA-differentiated monocytic cell lines and primary BMDMs transduced with SOCS1 or shSOCS1, the LPS/TLR4-induced inflammatory signaling was investigated by analyzing the levels of intracellular ROS, antioxidant factors, inflammasome activation, and pro-inflammatory cytokines. The levels of LPS-induced ROS and the production of pro-inflammatory cytokines were notably down-regulated by SOCS1 and up-regulated by shSOCS1 in an NAC-sensitive manner. SOCS1 up-regulated an ROS-scavenging protein, thioredoxin, via enhanced expression and binding of NRF-2 to the thioredoxin promoter. SOCS3 exhibited similar effects on NRF-2/thioredoxin induction, and ROS downregulation, resulting in the suppression of inflammatory cytokines. Notably thioredoxin ablation promoted NLRP3 inflammasome activation and restored the SOCS1-mediated inhibition of ROS and cytokine synthesis induced by LPS. The results demonstrate that the anti-inflammatory mechanisms of SOCS1 and SOCS3 in macrophages are mediated via NRF-2-mediated thioredoxin upregulation resulting in the downregulation of ROS signal. Thus, our study supports the anti-oxidant role of SOCS1 and SOCS3 in the exquisite regulation of macrophage activation under oxidative stress.