• Membrane and Water Treatment
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• v.3 no.2
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• pp.133-140
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• 2012

The overall performance of BNR-MBR, so-called Anoxic-Anaerobic-Aerobic Membrane Bioreactor ($A^3$-MBR), developed for nutrient removal was studied to determine the efficiencies and mechanisms under different solid retention time (SRT). The reactor was fed by synthetic high-rise building wastewater with a COD:N:P ratio of 100:10:2.5. The results showed that TKN, TN and phosphorus removal by the system was higher than 95%, 93% and 80%, respectively. Nitrogen removal in the system was related to the simultaneous nitrification-denitrification (SND) reaction which removed all nitrogen forms in aerobic condition. SND reaction in the system occurred because of the large floc size formation. Phosphorus removal in the system related to the high phosphorus content in bacterial cells and the little effects of nitrate nitrogen on phosphorus release in the anaerobic condition. Therefore, high quality of treated effluent could be achieved with the $A^3$-MBR system for various water reuse purposes.

• Journal of Marine Bioscience and Biotechnology
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• v.8 no.1
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• pp.24-29
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• 2016

Microalgae are considered as superior biodiesel producers, because they could effectively produce high amount of lipid with fast growth rate. In this study, Chlorella vulgaris was exposed to various light wavelengths (${\lambda}_{max}$ 470 nm, ${\lambda}_{max}$ 525 nm, and ${\lambda}_{max}$ 660 nm) using light emitting diodes (LEDs) to examine effect of light quality on their growth and fatty acid production in 0.4-L bubble column photobioreactors. Fluorescent lamps were also used as polychromatic light sources (control). From the results, biomass productivity was varied by light wavelength from 0.05 g/L/day to 0.30 g/L/day. Maximum biomass productivity was obtained from red LED among tested ones. We also observed that contents of oleic acid and linolenic acid, which affect biodiesel properties, were significantly changed depending on supplied wavelength. These results indicated that production of algal biomass, and fatty acid content and productivity could be improved or controlled by supplying specific light wavelength.

• KSBB Journal
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• v.31 no.1
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• pp.79-84
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• 2016

Biofuel has been considered as promising renewable energy to solve various problems that result from increasing usage of fossil fuels since the early 20th century. In terms of chemical and physical properties as fuel, biobutanol has more merits than bioethanol. It could replace gasoline for transportation and industrial demand is increasing significantly. Production of butanol can be achieved by chemical synthesis or by microbial fermentation. The water hyacinth, an aquatic macrophyte, originated from tropical South America but is currently distributed all over the world. Water hyacinth has excellent water purification capacity and it can be utilized as animal feed, organic fertilizer, and biomass feedstock. However, it can cause problems in the rivers and lakes due to its rapid growth and dense mats formation. In this study, the potential of water hyacinth was evaluated as a lignocellulosic biomass feedstock in biobutanol fermentation by using Clostridium beijerinckii. Water hyacinth was converted to water hyacinth hydrolysate medium through pretreatment and saccharification. It was found that productivity of water hyacinth hydrolysate medium on biobutanol production was comparable to general medium.

• KSBB Journal
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• v.26 no.5
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• pp.365-373
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• 2011

Effective Microorganisms (EM), a fermented medium developed by Professor Higa at the University of the Ryukyus, is a mixed culture containing dozens of microorganisms which are beneficial to nature including people, animals, plants and many microbial species in environment. EM is known to contain more than 80 kinds of anaerobic or aerobic microbes including photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, fungi and so on, with yeast, lactic acid bacteria and photosynthetic bacteria as the main species of EM. Antioxidant effect generated by the concert of complex coexistence and coprosperity among these microbes is considered to be the main source of EM benefits. Currently, EM is earning an increasing attention with applications in agriculture, forestry, animal husbandry, fisheries, environment and medicine among others. At the same time, however, a quantitative interpretation of EM system based on a mixed culture model needs efforts from biochemical engineers for efficient production and further promotion of EM. In this paper, we describe the functions of major microbes in EM and current researches and applications of EM in agriculture, forestry, animal husbandry, fisheries, environment and medicine.

• KSBB Journal
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• v.27 no.1
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• pp.51-56
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• 2012

This research was to investigate the polyester preparation using waste ethylene glycol (EG) generated from the wastepaper pretreatment process. Waste EG was obtained from using EG five times repeatedly in the pretreatment of wastepaper. The hydroxyl value of the waste EG was 441 mg KOH/g and its composition was 0.68% cellulose, 6.5% hemicellulose, 6.1% lignin, and 86.7% EG. Maleic acid was used as carboxylic acid. The effect of reaction temperature and time except carboxyl group/hydroxyl group ratio on the crosslinkage of the prepared polyester was marginal. Citric acid, lithium hydroxide and dicumyl peroxide were used as additive or catalyst to enhance the crosslinkage of polyester. Among them, 10% of citric acid was found to be most effective. The crosslinkage was 86% when the polyester was prepared at an optimum condition such as $130^{\circ}C$ and 15 minutes, 1.5 of C/H ratio, and 10% of citric acid, and its insoluble percentage in boiling water for 6 hours was 47%. The weight loss of the prepared polyester was approximately 40% when it was buried in damp soil for 5 months, indicating that it is readily biodegradable. This results can provide some information for future development of wastepaper pretreatment by organic solvent.

• Journal of the Korean Institute of Gas
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• v.12 no.3
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• pp.1-6
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• 2008

The safety management of the LNG industry which shows huge growth recently, become an essential element. So the necessity of development for the pre-existing LNG storage/transport facility has been shown up and the improvement of information technology (IT) of these days make it possible to synthesize several models for integrated LNG facilitiy safety management system. This system will contains risk analysis/assessment technology, explosion, leakage and diffusion model construction technology, real-time monitoring and fault diagnosis technology, and reliability progression technology of process information through data reconciliation. The final integrated safety management system will contribute the increase of LNG industry's safety and exportation of technique.

• Journal of the Korea Institute of Military Science and Technology
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• v.20 no.6
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• pp.853-859
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• 2017

Terrorists always threats the global security with the possibility of using prohibited warfare, NBCs(Nuclear, Biological and Chemical Warfare). Compared to other prohibited warfares, most of biological warfare agents (BWAs) have no physical properties and time delays from spread to affect. Therefore the early detection is important to protect and decontaminate from BWAs. On the preliminary detection stage for suspicious material, most of detection kits only serve to know weather the BWAs exists or not. Due to this reason, simple field confirmation testing for suspicious substances have been used to identify materials which show negative result on detection kits. Considering the current Lab on a Chip(LOC) technologies, we suggest simple identification platform for unknown suspicious substances based on paper fluidics. We hope that our research will envision the future direction for the specific point-of-view for LOC technologies on detection strategy of BWAs.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.913-917
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• 2008

The cyclic undecapeptide, cyclosporin A (CyA), is one of the most commonly prescribed immunosuppressive drugs. It is generated nonribosomally from a multifunctional cyclosporin synthetase enzyme complex by the filamentous fungus Tolypocladium niveum. In order to maximize the production of CyA by wild-type T. niveum (ATCC 34921), each of three culture stages (sporulation culture, growth culture, and production culture) were sequentially optimized. Among the three potential sporulation media, the SSMA medium generated the highest numbers of T. niveum spores. The SSM and SM media were then selected as the optimal growth and production culture media, respectively. The addition of valine and fructose to the SM production medium was also determined to be crucial for CyA biosynthesis. In this optimized three-stage culture system, 3% of the spore inoculum generated the highest level of CyA productivity in a 15-day T. niveum production culture, thereby implying that the determination of an appropriate size of T. niveum spore inoculum plays a critical role in the maximization of CyA production.

• Korea-Australia Rheology Journal
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• v.15 no.2
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• pp.91-96
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• 2003

The sensitivity of the product to the ongoing sinusoidal disturbances of the process has been investigated in the film casting of viscoelastic polymer fluids using frequency response analysis. As demonstrated for fiber spinning process (Jung et al., 2002; Devereux and Denn, 1994), this frequency response analysis is useful for examining the process sensitivity and the stability of extensional deformation processes including film casting. The results of the present study reveal that the amplification ratios or gains of the process/product variables such as the cross-sectional area at the take-up to disturbances exhibit resonant peaks along the frequency regime as expected for the systems having hyperbolic characteristics with spilt boundary conditions (Friedly, 1972). The effects on the sensitivity results of two important parameters of film casting, i.e., the fluid viscoelasticity and the aspect ratio of the casting equipment have been scrutinized. It turns out that depending on the extension thinning or thickening nature of the fluid, increasing viscoelasticity results in enlargement or reduction of the sensitivity, respectively. As regards the aspect ratio, it has been found that an optimum value exists making the system least sensitive. The present study also confirms that the frequency response method produces results that corroborate well those by other methods like linear stability Analysis and transient solutions response. (Iyengar and Co, 1996; Silagy et al., 1996; Lee and Hyun, 2001).

• ALGAE
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• v.24 no.2
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• pp.121-127
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• 2009

The unicellular green alga, Haematococcus pluvialis, accumulates the highest level of astaxanthin among knownastaxanthi.n-producing organisms. Light is the most important factor to induce astaxanthin by H. pluvialis. BIue andred LEDs, whose ${\lambda}_{max}$'s are 470 and 665 nm, respectively, were used for internally illuminated light sources.Fluorescent lamps were also used for both internal and external illumination sources. The astaxanthin levels in thesevarious lighting systems were analyzed and compared each other. The cultures under internally illuminated LEDsaccumulaled 20% more astaxanthin than those under fluorescent lamp. Furthermore, LEDs generated much lessheat than the fluorescent lamps, which gives one more reason for the LEDs being a suitable internal Light source forastaxanthin induction. The results reported here would lead novel designs of photobioreactors with improvementsof illumination methods for high level of astaxanthm production. The maximum astaxanthin concentrations as wellas the astaxanthin yield per supplied photon were increased by at least 20% when blue or red LEDs were supplied.