• Natural Product Sciences
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• 제19권4호
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• pp.303-310
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• 2013

Velvet deer antler (VDA) is well known oriental medicine claimed to have tonic activities as improving bone mineral density (BMD), immune-enhancing, rejuvenating and many other medicinal activities. Ossified deer antler (ODA) is bony product produced by over-calcification of deer antler due to late harvesting. The extraction efficiency of ODA by conventional boiling in water must be very poor due to bony nature, hence the reputations for the medicinal efficacies of ODA has been highly under-evaluated compared to that of VDA without any experimental evidences. Employing our new efficient water extraction process ($135^{\circ}C$), the extracts of ODA and VDA were analysed to compare the contents of bioactive components and the potencies of pharmacological activities. The results showed that; 1) The $135^{\circ}C$ extraction (autoclaving) of ODA gave highly increased amount of biomass, 120% more than the conventional extraction by 100-boiling, whereas the same treatment for VDA showed only 15% increased amount of biomass. 2) Feeding the ODA- or VDA-extracts to oophorectomized rats showed very potent BMD-recovering activity. 3) During the ossification of deer antler, the total collagen content was found to be increased by addition of type-1 to pre-existing type-2 collagen, but not replacement of type-2 to type-1 collagen. High titer of peptide hormones like growth hormone and IGF-1 were detected in the ODA- and VDA-extracts and also in the serum of ODA- or VDA-treated oophorectomized animals dose-dependently. Present experimental data will give a conclusion that folkloric poor reputations on ODA must be concerned only with poor extraction efficiency of conventional $100^{\circ}C$ water extraction and not based on the composition of bioactive substances of ODA.

• Journal of Dairy Science and Biotechnology
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• 제31권1호
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• pp.75-83
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• 2013

Mare milk is gaining importance because of its nutritional characteristics and therapeutic properties, which enable its use as part of the diet of the elderly, convalescents, and newborn infants. This review describes the functional and bioactive components of mare milk, such as proteins, carbohydrates, and lipids, and the characteristics such as acidification and released free amino acids of fermented mare milk. The protein profile of mare milk differs from that of bovine milk but is similar to that of human milk. The salt and lactose content in mare's milk is similar to that in human milk, but mare's milk has a significantly lower content of fat. Whey protein concentration is higher and casein content is much lower in mare milk than in bovine milk. These health-promoting properties indicate that mare milk and its derivatives could become valuable foods for elderly consumers in the form of probiotic beverages. Protein allergies related to and the potential industrial applications of mare milk have also been discussed in comparison with those of bovine milk. Although mare milk has diverse advantages if used as a nutritional food and has positive effects on health, further studies are required to enable its use as a complete substitute for human milk or as a health food.

• 분석과학
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• 제35권2호
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• pp.53-59
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• 2022

Mugwort (Artemisia montana), which is a perennial plant mainly distributed throughout Northeast Asian regions, has been used as a preferred source of various foods and traditional medicines in Korea. In particular, as essential oils extracted from mugwort were reported to be biologically active, its steam distillate has been widely used to treat various conditions, such as itching, hemorrhoids, and gynecological inflammation. Therefore, efforts have been devoted to develop effective methods for the collection of bioactive essential oils from mugwort. In this study, five mugwort extracts were obtained using different extraction conditions, namely, 6 % ethanol at room temperature and at 80 ℃, pure ethanol, n-hexane, and an adsorbent resin. To evaluate the five extracts of mugwort, area-under-the-curve values (AUCs), chemical profiles, and major bioactive essential oil contents were investigated using gas chromatography-mass spectrometry (GC-MS). An overall assessment of the volatile components, including essential oils, in the five extracts was conducted using AUCs, and the individual essential oil in each extract was identified. Furthermore, the four major essential oils (1,8-cineole, camphor, borneol, and α-terpineol), which are known to possess anti-microbial and anti-inflammatory activities, were quantified using authentic chemical standards. Based on the evaluation results, pure ethanol was the best extractant out of the five used in this study. This study provides evaluation results for the five different mugwort extracts and would be helpful for developing extraction methods to efficiently collect the bioactive oil components for medical purposes using chemical profiles of the extracts.

• Natural Product Sciences
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• 제20권2호
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• pp.102-106
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• 2014

Gumiganghwal-tang (GMT) is a traditional herbal prescription used for treatment of the common cold, pain, and inflammatory diseases. Variations in the amounts of bioactive components of GMT and GMT fermented with 10 Lactobacillus strains were investigated by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). Simultaneous qualitative and quantitative analyses of eleven bioactive compounds (prim-O-glucosylcimifugin, liquiritin, cimifugin, baicalin, liquiritigenin, wogonoside, baicalein, wogonin, butylphthalide, imperatorin, and isoimperatorin) were performed, with comparison of their retention times (tR) and UV spectra with those of standard compounds. The amounts of baicalin (8.71 mg/g), liquiritigenin (5.28 mg/g) and butylphthalide (5.10 mg/g) were the major compounds in GMT. We found that L. fermentum KFRI 145 fermented wogonoside and baicalin to their aglycones, wogonin and baicalein, respectively. These results indicated that L. fermentum KFRI 145 has potential as a functional starter culture for manufacturing fermented GMT.

• Journal of Ginseng Research
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• 제48권4호
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• pp.354-365
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• 2024

Panax species include Panax ginseng Meyer, Panax quinquefolium L., Panax notoginseng, Panax japonicum, Panax trifolium, and Panax pseudoginseng, which contain bioactive components (BCs) such as ginsenosides and polysaccharides. Recently, growing evidence has revealed the pharmacological effects of Panax species and their BCs on allergic airway diseases (AADs), including allergic asthma (AA) and allergic rhinitis (AR). AADs are characterized by damaged epithelium, sustained acquired immune responses with enforced Th2 responses, allergenspecific IgE production, and enhanced production of histamine and leukotrienes by activated mast cells and basophils. In this review, we summarize how Panax species and their BCs modulate acquired immune responses involving interactions between dendritic cells and T cells, reduce the pro-inflammatory responses of epithelial cells, and reduce allergenic responses from basophils and mast cells in vitro. In addition, we highlight the current understanding of the alleviative effects of Panax species and their BCs against AA and AR in vivo. Moreover, we discuss the unmet needs of research and considerations for the treatment of patients to provide basic scientific knowledge for the treatment of AADs using Panax species and their BCs.

• 약학회지
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• 제34권4호
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• pp.277-281
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• 1990

The isolation and identification of an antibacterial component, from Polygoni Radix against a cariogenic bacterium Streptococcus mutans OMZ 176, were carried out for development of anticariogenic agents. The bioactive component was identified to be emodin. The minimal inhibitory concentration (MIC) of emodin was $100\;{\mu}g/ml$ against S. mutans OMZ 176. The bioactive component emodin weakly inhibited ${\beta}-lactamase$ activity with the inhibition ratio of 1.7, 4.3 and 7.6% at the concentration of 50, 100, and 200 uM, respectively. Emodin exhibited slight phototoxicity when analysed by the photohemolysis method.

• Journal of Dairy Science and Biotechnology
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• 제28권2호
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• pp.19-24
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• 2010

Milk components and their derivatives are a rich source of bioactive substances and play a central role in the development of functional foods. Many scientific evidences have shown that milk proteins have a biological health-promoting functionality. However, in recent times, dairy calcium and growth factors derived from milk have been misunderstood to cause diseases such as osteoporosis or cancer. This review focuses on the importance of milk-derived growth factors and some minor active components and briefly considers the emerging trends of personalized nutrition in future dairy research and development.

• 생명과학회지
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• 제28권2호
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• pp.229-239
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• 2018

안전하고 건강지향적인 식품에 관한 소비자들의 관심이 증가함에 따라, 학계와 산업계에서는 생리활성 기능을 가진 소재를 이용한 식품을 개발하려는 시도가 많이 이루어지고 있는 추세이다. 특히 합성물질보다는 생리활성작용을 하는 천연물질의 이용이 증가되고 있는데 이러한 이유는 합성화학물질과 달리 천연물질은 그 이용에 대한 제한이 없고 소비자들의 신뢰도가 높기 때문이다. 폐계육의 가슴살과 명태살을 이용한 게맛살을 제조할 때 동충하초분말, 누에고치분말 및 CLA의 혼합 첨가가 게맛살의 이화학적 품질특성에 미치는 효과를 구명하기 위하여 폐계육 회수단백질과 명태살에 누에고치분말(10 g)을 첨가한 T1, 누에고치분말(5 g)과 동충하초분말(5 g)을 첨가한 T2, 동충하초분말(5 g)과 공액리놀랜산(5 g)(CLA)를 첨가한 T3를 제조하여 실험을 수행한 결과를 요약하면 다음과 같다. pH와 전단가는 저장초기에 비교해 저장 2주 이후에 모든 처리구에서 유의적으로 증가하는 경향을 나타내었다. 전단가는 6주에서 동충하초분말과 CLA를 혼합 첨가한 구(T3)에서 유의적으로 높게 나타났다. 육색의 경우 적색도($a^{\ast}$)와 백색도(W)는 저장기간 동안 모든 구간에서 높게 나타난 반면에 황색도($b^{\ast}$)는 저장기간 동안 감소하였다. T2의 적색도($a^{\ast}$), 황색도($b^{\ast}$)가 다른 처리구보다 상당히 높게 나타내었다. 생리활성물질의 첨가는 게맛살의 겔 특성과 조직감에 영향을 미치지 않았다. 지방산패도(TBARS)와 총균수(TPC)는 T1이 다른 처리구보다 상당히 높게 나온 반면에 단백질변패도(VBN)의 경우 다른 처리구보다 T2가 높게 나왔다. T1과 T3는 저장기간 동안 총 아미노산함량이 감소하였다. 결과적으로 동충하초분말 0.5%와 CLA 0.5% 혼합 첨가한 T3 처리구가 종합적인 품질면에서 폐계육 함유 게맛살의 기능성을 가장 높일 수 있을 것으로 사료된다.

• 한국버섯학회지
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• 제20권3호
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• pp.119-126
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• 2022

전염병확산에 따라 건강에 대한 중요성이 높아지면서, 기능성이 소비자들의 버섯 구매에 중요한 요소로 작용하고 있다. 양송이 소비촉진을 위한 기능성 정보를 알리기 위해 백색 느티만가닥버섯를 대조구로 백색양송이와 갈색양송이로 구분하여 영양성분 및 생리활성 성분을 비교 분석하였다. 백색양송이는 갈색양송이에 비해 당도와 무기성분인 칼륨이 높았으며, 나트륨과 마그네슘, 비타민 C는 갈색양송이와 유의적인 차이가 없었다. 또한 Ergosterol 함량은 백색양송이가 갈색양송이 대비 약 2배정도 높은 것으로 확인되었다. 갈색양송이는 백색양송이에 비해 𝛽-Glucan, 유기산인 oxalic acid가 높았지만, 총 유기산 함량은 유의적 차이가 없었다. 또한, 아미노산은 Ergothionein, Isoleucine, Leucine, Glycine, Alanine, 비타민은 B 그룹, 그리고 총 폴리페놀 함량이 갈색양송이가 백색양송이보다 높았다.

• Journal of Ginseng Research
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• 제33권3호
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• pp.165-176
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• 2009

Korean ginseng, which contains ginsenosides and polysaccharides as its main constituents, is orally administered to humans. Ginsenosides and polysaccharides are not easily absorbed by the body through the intestines due to their hydrophilicity. Therefore, these constituents which include ginsenosides Rb1, Rb2, and Rc, inevitably come into contact with intestinal microflora in the alimentary tract and can be metabolized by intestinal microflora. Since most of the metabolites such as compound K and protopanaxatriol are nonpolar compared to the parental components, these metabolites are easily absorbed from the gastrointestinal tract. The absorbed metabolites may express pharmacological actions, such as antitumor, antidiabetic, anti-inflammatory, anti-allergic, and neuroprotective effects. However, the activities that metabolize these constituents to bioactive compounds differ significantly between individuals because all individuals possess characteristic indigenous strains of intestinal bacteria. Recently, ginseng has been fermented with enzymes or microbes to develop ginsengs that contain these metabolites. However, before using these enzymes and probiotics, their safety and biotransforming activity should be assessed. Intestinal microflora play an important role in the pharmacological action of orally administered ginseng.