• Title/Summary/Keyword: Bio-system

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Future Directions and Perspectives on Soil Environmental Researches (토양환경분야 연구동향 및 전망)

  • Yang, Jae-E.;Ok, Yong-Sik;Chung, Doug-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.6
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    • pp.1286-1294
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    • 2011
  • This paper reviews the future directions and perspectives on the soil environmental researches in the 21 century. Previously, the principal emphasis of soil environmental researches had put on the enhancement of food and fiber productions. Beside the basic function of soil, however, the societal needs on soil resources in the 21st century have demands for several environmental and social challenges, occurring regionally or globally. Typical global issues with which soil science should deal include food security with increasing agronomic production to meet the exploding world population growth, adaptation and mitigation of climate change, increase of the carbon sequestration, supply of the biomass and bioenergy, securing the water resource and quality, protection of environmental pollution, enhancing the biodiversity and ecosystem health, and developing the sustainable farming/cropping system that improve the use efficiency of water and agricultural resources. These challenges can be solved through the sustainable crop production intensification (SCPI) or plant welfare concept in which soil plays a key role in solving the abovementioned global issues. Through implementation of either concept, soil science can fulfill the goal of the modern agriculture which is the sustainable production of crops while maintaining or enhancing the ecosystem function, quality and health. Therefore, directions of the future soil environmental researches should lie on valuing soil as an ecosystem services, translating research across both temporal and spatial scales, sharing and using data already available for other purposes, incorporating existing and new technologies from other disciplines, collaborating across discipline, and translating soil research into information for stakeholders and end users. Through the outcomes of these approaches, soil can enhance the productivity from the same confined land, increase profitability, conserve natural resource, reduce the negative impact on environment, enhance human nutrition and health, and enhance natural capital and the flow of ecosystem services. Soil is the central dogma, final frontier and new engine for the era of sustainability development in the $21^{st}$ century and thus soil environmental researches should be carried according to this main theme.

Contents of Soil Microbial Phospholipid Fatty Acids as Affected by Continuous Cropping of Pepper under Upland (노지 고추 연작 토양의 미생물 인지질 지방산 함량)

  • Hwang, Jae-Moon;Park, Kee-Choon;Kim, Su-Jung
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.1012-1017
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    • 2010
  • This study was carried out to investigate the effect of continuous cropping of pepper on soil microbial phospholipid fatty acids (PLFAs) under upland applied without any pesticides and chemical herbicides from 2000 to 2009. Microbial PLFAs were analysed from soils sampled in 2009. Soil microbial diversities showed PLFAs of monoplanting of pepper were distinct from those of monoplanting of garlic and interplanting of garlic and pepper by principle component 2 (PC2). Furthermore, soil microbial activity of monoplanting of pepper significantly decreased PLFAs representing as VAM-fungi, whereas it significantly increased in actinomycetes and saturated/monounsaturated PLFAs' ratio. The results drove continuous cropping of pepper would vary the microbial community and their specific activity. Soil microbial activities in continuous cropping system would depend on crop root systems.

Cell Disruption of Dunaliella salina using Batch Low Frequency Non-Focused Ultrasound (비집속 회분저주파를 이용한 Dunaliella salina 세포 파쇄)

  • Choi, Jun-Hyuk;Kim, Gwang-Ho;Park, Jong-Rak;Jeong, Sang-Hwa
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.20 no.10
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    • pp.63-71
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    • 2021
  • Using fossil fuels in existing industrial systems causes a variety of social problems. Recently, many studies have been conducted on bio-refineries, which aim to actively utilize biomass to reduce the use of fossil fuels and solve various social problems. Among them, research using microalgae as a third-generation biomass has attracted considerable attention. Microalgae use inorganic matter to produce organic matter, and cell destruction is necessary to extract useful organic materials from microalgae. The extracted organic materials are currently used in various industrial fields. Numerous cell-destruction methods exist. We have investigated cell disruption by sonication, especially its efficiency. Ultrasound is a sound wave with frequencies above 20 kHz, and destroys cells by sending high energy through a cavitation that occurs, according to the characteristics of the sound wave. The Dunaliella salina microalgae used in this study was cultured in a flat-type photobioreactor. Experiments were performed using a batch low-frequency processing device. Logistic model was applied to analyze the results of cell-destruction experiments using ultrasound. The proper conditions for the most efficient cell destruction were OD 1.4(microalgae concentration)), 54watt(output power) and 200mL(microalgae capacity).

Analysis of Flood Control Capacity of Agricultural Reservoir Based on SSP Climate Change Scenario (SSP 기후변화 시나리오에 따른 농업용 저수지 홍수조절능력 분석)

  • Kim, Jihye;Kwak, Jihye;Hwang, Soonho;Jun, Sang Min;Lee, Sunghack;Lee, Jae Nam;Kang, Moon Seong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.63 no.5
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    • pp.49-62
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    • 2021
  • The objective of this study was to evaluate the flood control capacity of the agricultural reservoir based on state-of-the-art climate change scenario - SSP (Shared Socioeconomic Pathways). 18 agricultural reservoirs were selected as the study sites, and future rainfall data based on SSP scenario provided by CMIP6 (Coupled Model Intercomparison Project 6) was applied to analyze the impact of climate change. The frequency analysis module, the rainfall-runoff module, the reservoir operation module, and their linkage system were built and applied to simulate probable rainfall, maximum inflow, maximum outflow, and maximum water level of the reservoirs. And the maximum values were compared with the design values, such as design flood of reservoirs, design flood of direct downstream, and top of dam elevation, respectively. According to whether or not the maximum values exceed each design value, cases were divided into eight categories; I-O-H, I-O, I-H, I, O-H, O, H, X. Probable rainfall (200-yr frequency, 12-h duration) for observed data (1973~2020) was a maximum of 445.2 mm and increased to 619.1~1,359.7 mm in the future (2011~2100). For the present, 61.1% of the reservoirs corresponded to I-O, which means the reservoirs have sufficient capacity to discharge large inflow; however, there is a risk of overflowing downstream due to excessive outflow. For the future, six reservoirs (Idong, Baekgok, Yedang, Tapjung, Naju, Jangsung) were changed from I-O to I-O-H, which means inflow increases beyond the discharge capacity due to climate change, and there is a risk of collapse due to dam overflow.

6-O-Galloylsalidroside, an Active Ingredient from Acer tegmentosum, Ameliorates Alcoholic Steatosis and Liver Injury in a Mouse Model of Chronic Ethanol Consumption

  • Kim, Young Han;Woo, Dong-Cheol;Ra, Moonjin;Jung, Sangmi;Kim, Ki Hyun;Lee, Yongjun
    • Natural Product Sciences
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    • v.27 no.3
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    • pp.201-207
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    • 2021
  • We have previously reported that Acer tegmentosum extract, which is traditionally used in Korea to reduce alcohol-related liver injury, suppresses liver inflammation caused by excessive alcohol consumption and might improve metabolism. The active ingredient, 6-O-galloylsalidroside (GAL), was isolated from A. tegmentosum, and we hypothesized that GAL could provide desirable pharmacological benefits by ameliorating physiological conditions caused by alcohol abuse. Therefore, this study focused on whether GAL could ameliorate alcoholic fat accumulation and repair liver injury in mice. During chronic alcohol consumption plus binge feeding in mice, GAL was administered orally once per day for 11 days. Intrahepatic lipid accumulation was measured in vivo using a noninvasive method, 1H magnetic resonance imaging, and confirmed by staining with hematoxylin and eosin and Oil Red O. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using a Konelab system, and the triglyceride content was measured in liver homogenates using an enzymatic peroxide assay. The results suggested that GAL alleviated alcohol-induced steatosis,e as indicated by decreased hepatic and serum triglyceride levels in ethanol-fed mice. GAL treatment also correlated with a decrease in the Cd36 mRNA expression, thus potentially inhibiting the development of alcoholic steatosis via the hepatic de novo lipogenesis pathway. Furthermore, treatment with GAL inhibited the expression of cytochrome P450 2E1 and attenuated hepatocellular damage, as reflected by a reduction in ALT and AST levels. These findings suggest that GAL extracted from A. tegmentosum has the potential to serve as a bioactive agent for the treatment of alcoholic fatty liver and liver damage.

Management of Food Allergy in the Facilities Registered at Center for Children's Foodservice Management in Gangdong-gu (강동구 어린이급식관리지원센터 등록 시설의 식품알레르기 관리 현황)

  • Kim, Soon Mi
    • Korean Journal of Community Nutrition
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    • v.26 no.5
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    • pp.396-407
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    • 2021
  • Objectives: We examined the common allergenic foods, symptoms and management of food allergies in children attending the facilities registered at Center for Children's Foodservice Management in Gangdong-gu, Korea. Methods: The survey was conducted among the directors or head teachers of 186 children's food service facilities with 7,591 children in 2019. The questionnaire consisted of three parts, including general information about food service facilities, information related to food allergies and allergenic foods and symptoms in individual children. Results: The number of children with food allergy was 271 (3.6%), and the proportion decreased with the increase of age. There were 91 children (33.6%) with a medical certificate, and these children had a significantly higher number of allergenic foods and symptoms than those without a medical certificate. Allergenic food groups included meat, fish, eggs and legumes (59.1%), fruits (12.4%), milk and dairy products (8.9%), cereals (7.8%), vegetables (6.2%), processed foods (3.8%) and oils and sugars (1.9%). Eggs accounted for 22.1%, followed by peanut and tree nuts (18.6%), fruits (12.4%), milk and dairy products (8.9%), shellfish (8.6%), vegetables (6.2%), fish (5.7%), cereals (4.3%) and meat (1.1%). The common allergenic foods were eggs, peanuts, walnuts, kiwi, shrimp, milk, tomatoes, mackerel, blue-green fish, peaches, shellfish (clams and abalone), buckwheat, wheat and soybeans. The most common allergic symptoms were skin and mucous membrane symptoms, such as hives, rash, itching and oral angioedema. Meal management for children with food allergies showed different trends depending on the causative food. Conclusions: The objective diagnosis by an allergist should be done for food allergy management in children's catering facilities. A system for systematic meal management of causative foods should be prepared.

Radiation measurement and imaging using 3D position sensitive pixelated CZT detector

  • Kim, Younghak;Lee, Taewoong;Lee, Wonho
    • Nuclear Engineering and Technology
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    • v.51 no.5
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    • pp.1417-1427
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    • 2019
  • In this study, we evaluated the performance of a commercial pixelated cadmium zinc telluride (CZT) detector for spectroscopy and identified its feasibility as a Compton camera for radiation monitoring in a nuclear power plant. The detection system consisted of a $20mm{\times}20mm{\times}5mm$ CZT crystal with $8{\times}8$ pixelated anodes and a common cathode, in addition to an application specific integrated circuit. The performance of the various radioisotopes $^{57}Co$, $^{133}Ba$, $^{22}Na$, and $^{137}Cs$ was evaluated. In general, the amplitude of the induced signal in a CZT crystal depends on the interaction position and material non-uniformity. To minimize this dependency, a drift time correction was applied. The depth of each interaction was calculated by the drift time and the positional dependency of the signal amplitude was corrected based on the depth information. After the correction, the Compton regions of each spectrum were reduced, and energy resolutions of 122 keV, 356 keV, 511 keV, and 662 keV peaks were improved from 13.59%, 9.56%, 6.08%, and 5%-4.61%, 2.94%, 2.08%, and 2.2%, respectively. For the Compton imaging, simulations and experiments using one $^{137}Cs$ source with various angular positions and two $^{137}Cs$ sources were performed. Individual and multiple sources of $^{133}Ba$, $^{22}Na$, and $^{137}Cs$ were also measured. The images were successfully reconstructed by weighted list-mode maximum likelihood expectation maximization method. The angular resolutions and intrinsic efficiency of the $^{137}Cs$ experiments were approximately $7^{\circ}-9^{\circ}$ and $5{\times}10^{-4}-7{\times}10^{-4}$, respectively. The distortions of the source distribution were proportional to the offset angle.

Manganese in Seawaters of the Amundsen Sea, Antarctic (남극 아문젠해에서 해수 중 Mn의 분포 특성)

  • Jang, Dongjun;Choi, Mansik;Park, Jongkyu;Park, Kyungkyu;Hong, Jinsol;Lee, Sanghoon;Jung, Jinyoung
    • Ocean and Polar Research
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    • v.41 no.2
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    • pp.63-77
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    • 2019
  • In order to investigate the behavior and seasonal variability of Mn as one of the bio-essential metals in the Amundsen sea, which is known as the most biologically productive coastal area around the Antartica, seawaters were collected using a clean sampling system for 10 stations (96 ea) in 2014 (ANA04B) and for 12 stations (139 ea) in 2016 (ANA06B) surveys of RV ARAON. Dissolved and particulate Mn concentration varied in the range of 0.15-4.43 nmol/kg and <0.01 to 2.42 nM in 2014 and in the range of 0.25-4.15 nmol/kg and 0.01-2.64 nM in 2016, respectively. From the sectional distribution of dissolved and particulate Mn, it might be suggested that dissolved/particulate Mn was provided from iceberg melting and diffusion/resuspension from sediments, respectively. Although this sea is highly productive, there was little evidence regarding the biological origin of dissolved Mn, but particulate Mn only in sea ice and offshore areas could be explained as originating from organic matters, e.g. phytoplanktons. And it could be suggested that the subsurface maximum of dissolved Mn was formed by isopycnal transport of melting materials from ice wall to offshore. Compared to early (2014) summer, temperature, salinity, biomass, dissolved and particulate Mn in late (2016) summer indicated that temporal variations might be resulted from the reduction of ice melting and mCDW flow, which induced a reduction in resuspension. In addition, in the late summer, particles including biomass were reduced, which brought about a reduction in the removal rate of dissolved Mn.

Design and manufacture of eyeball protrusion measuring device using white light scanning interferometer (백색광 간섭계를 이용한 안구 돌출 측정 장치 설계 및 제작)

  • Chang, Jung-soo;Kim, Young-kil
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.23 no.1
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    • pp.63-69
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    • 2019
  • The relative position of the orbital eye can be a criterion for evaluating several pathological conditions. It is especially useful to diagnose orbital fractures, thyroid eye disease, orbital tumors and to evaluate the outcome of medication and surgical treatment. Hertel and Naugle are representative measurement tools used to measure eyeball protrusion values, and have different measurement results, such as fixed orbits, every time they are inspected, even if the same inspector repeatedly measures them. Even with the same calibrator, it is inevitable that different manufacturers will change the design of the stationary part of the orbit, causing the surveyor to make a measurement error. In this paper, we designed and fabricated a protrusion measuring device using a white light interferometer and measured the protrusion of the human eye and found that the precision and repeatability were significantly higher than the manual measurement method.

Generation of a Human Monoclonal Antibody to Cross-Reactive Material 197 (CRM197) and Development of a Sandwich ELISA for CRM197 Conjugate Vaccines

  • Kim, Dain;Yoon, Hyeseon;Kim, Sangkyu;Wi, Jimin;Chae, Heesu;Jo, Gyunghee;Yoon, Jun-Yeol;Kim, Heeyoun;Lee, Chankyu;Kim, Se-Ho;Hong, Hyo Jeong
    • Journal of Microbiology and Biotechnology
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    • v.28 no.12
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    • pp.2113-2120
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    • 2018
  • Cross-reactive material 197 ($CRM_{197}$) is a non-toxic mutant of diphtheria toxin containing a single amino acid substitution of glycine 52 with glutamic acid. $CRM_{197}$ has been used as a carrier protein for poorly immunogenic polysaccharide antigens to improve immune responses. In this study, to develop a sandwich ELISA that can detect $CRM_{197}$ and $CRM_{197}$ conjugate vaccines, we generated a human anti-$CRM_{197}$ monoclonal antibody (mAb) 3F9 using a phage-displayed human synthetic Fab library and produced mouse anti-$CRM_{197}$ polyclonal antibody. The affinity ($K_D$) of 3F9 for $CRM_{197}$ was 3.55 nM, based on Bio-Layer interferometry, and it bound specifically to the B fragment of $CRM_{197}$. The sandwich ELISA was carried out using 3F9 as a capture antibody and the mouse polyclonal antibody as a detection antibody. The detection limit of the sandwich ELISA was <1 ng/ml $CRM_{197}$. In addition, the 3F9 antibody bound to the $CRM_{197}$-polysaccharide conjugates tested in a dose-dependent manner. This ELISA system will be useful for the quantification and characterization of $CRM_{197}$ and $CRM_{197}$ conjugate vaccines. To our knowledge, this study is the first to generate a human monoclonal antibody against $CRM_{197}$ and to develop a sandwich ELISA for $CRM_{197}$ conjugate vaccines.