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http://dx.doi.org/10.14775/ksmpe.2021.20.10.063

Cell Disruption of Dunaliella salina using Batch Low Frequency Non-Focused Ultrasound  

Choi, Jun-Hyuk (Dept. of Mechanical Engineering, CHOSUN Univ.)
Kim, Gwang-Ho (Dept. of Mechanical System, Gwangju Campus of Korea Polytechnics)
Park, Jong-Rak (Dept. of Photonic Engineering, CHOSUN Univ.)
Jeong, Sang-Hwa (Dept. of Mechanical Engineering, CHOSUN Univ.)
Publication Information
Journal of the Korean Society of Manufacturing Process Engineers / v.20, no.10, 2021 , pp. 63-71 More about this Journal
Abstract
Using fossil fuels in existing industrial systems causes a variety of social problems. Recently, many studies have been conducted on bio-refineries, which aim to actively utilize biomass to reduce the use of fossil fuels and solve various social problems. Among them, research using microalgae as a third-generation biomass has attracted considerable attention. Microalgae use inorganic matter to produce organic matter, and cell destruction is necessary to extract useful organic materials from microalgae. The extracted organic materials are currently used in various industrial fields. Numerous cell-destruction methods exist. We have investigated cell disruption by sonication, especially its efficiency. Ultrasound is a sound wave with frequencies above 20 kHz, and destroys cells by sending high energy through a cavitation that occurs, according to the characteristics of the sound wave. The Dunaliella salina microalgae used in this study was cultured in a flat-type photobioreactor. Experiments were performed using a batch low-frequency processing device. Logistic model was applied to analyze the results of cell-destruction experiments using ultrasound. The proper conditions for the most efficient cell destruction were OD 1.4(microalgae concentration)), 54watt(output power) and 200mL(microalgae capacity).
Keywords
Cell Disruption; Microalgae; Ultrasound; Cell Membrane; Logistic Model;
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