• Title/Summary/Keyword: Bio-pigment

Search Result 61, Processing Time 0.028 seconds

Studies on the Red Pigment Produced by Monascus sp. in Submerged Culture. Part II Production of Crude Pigment, Physical and Physiological Characteristics. (액침 진탕 배양에 의한 Monascus sp.가 생산하는 적색 색소에 관한 연구 제2보 적색조색소의 생산과 물리적 성질 및 생리적 성질)

  • 김현수;곽효성;양호석;변유량;유주현
    • Microbiology and Biotechnology Letters
    • /
    • v.7 no.1
    • /
    • pp.31-36
    • /
    • 1979
  • Yellow pigment was extracted by petroleum ether, and red pigment by 60% ethanol. By thin layer chromatography red pigment preparation consists of more than five species whereas yellow pigment preparation consist of single species. The absorption curve of pigment solution exhibits maximum peak at wavelongth range of 495~500 nm and endo pigment at 394~403 nm. Pigment solution was relatively stable at the pH range of 3~9. Extracted pigment solution gave negative hemolysis test and pigment showed no bio-activity and nontoxicity.

  • PDF

Wnt5a attenuates the pathogenic effects of the Wnt/β-catenin pathway in human retinal pigment epithelial cells via down-regulating β-catenin and Snail

  • Kim, Joo-Hyun;Park, Seoyoung;Chung, Hyewon;Oh, Sangtaek
    • BMB Reports
    • /
    • v.48 no.9
    • /
    • pp.525-530
    • /
    • 2015
  • Activation of the Wnt/β-catenin pathway plays a pathogenic role in age-related macular degeneration (AMD) and is thus a potential target for the development of therapeutics for this disease. Here, we demonstrated that Wnt5a antagonized β-catenin response transcription (CRT) induced with Wnt3a by promoting β-catenin phosphorylation at Ser33/Ser37/Thr41 and its subsequent degradation in human retinal pigment epithelial (RPE) cells. Wnt5a decreased the levels of vascular endothelial growth factor (VEGF), tumor necrosis factor-α(TNF-α), and nuclear factor-κB (NF-κB), which was up-regulated by Wnt3a. Furthermore, Wnt5a increased E-cadherin expression and decreased cell migration by down-regulating Snail expression, thereby abrogating the Wnt3a-induced epithelial-mesenchymal transition (EMT) in human RPE cells. Our findings suggest that Wnt5a suppresses the pathogenic effects of canonical Wnt signaling in human RPE cells by promoting β-catenin phosphorylation and degradation. Therefore, Wnt5a has significant therapeutic potential for the treatment of AMD. [BMB Reports 2015; 48(9): 525-530]

Effects of Incubation Temperature on Egg Development, Hatching and Pigment Plug Evacuation in Farmed Siberian Sturgeon Acipenser baerii

  • Park, Chulhong;Lee, Sang Yoon;Kim, Dong Soo;Nam, Yoon Kwon
    • Fisheries and Aquatic Sciences
    • /
    • v.16 no.1
    • /
    • pp.25-34
    • /
    • 2013
  • Early ontogenic development in Siberian sturgeon Acipenser baerii was documented and the effects of different temperatures on embryonic and prelarval development were examined. Photograph-assisted data on morphogenesis in Siberian sturgeon prolarvae agreed well with published descriptions of their ontogeny and ecological behaviors, although certain aspects of differentiation, such as gill covering and scute development, could be rearing condition-sensitive. The present study provides the first characterization of the transient development of teeth during early larval stages; the pattern was congruent with the transition of prolarvae to exogenous feeding. From examinations of embryonic and prelarval development under different temperature conditions ($12-24^{\circ}C$), developmental speed was inversely related with temperature. Overall, hatchability was higher and hatching events were more synchronized at $20^{\circ}C$ than at lower temperatures. After hatching, similar patterns of temperature-dependency were observed in yolk sac absorption and the evacuation of the pigment plug. Our results suggest that the incubation of Siberian sturgeon embryos and prolarvae at temperatures close to $20^{\circ}C$ would be advantageous in hatcheries, based on reductions in the duration and uniformity of egg and prolarval developmental stages.

Characteristics and Stability of Violet Red Pigment Extracted from Salicornia herbacea L. (퉁퉁마디(Salicornia herbacea L.)에서 추출한 적자색 색소의 특성 및 안정성)

  • Lee, Young-Jae;Park, In-Bae;Kim, Hae-Seop;Shin, Gung-Won;Park, Jeong-Wook;Jo, Yeong-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.38 no.7
    • /
    • pp.885-891
    • /
    • 2009
  • To examine stability of a violet red pigment in a Salicornia herbacea L., several tests for pH, sugar, organic acid, ascorbic acid, amino acid, salt and temperature effect were carried out. A violet red pigment in Salicornia herbacea L. was the most stable in the range of pH 4 to 6. Each of the violet red pigments in Salicornia herbacea L. added with different amounts of sugar showed higher retention rate according to storage time than those of the control group. After 10 days of storage, especially the retention rate of the experimentals with 0.5 M sucrose was higher than the controls by about 11.1% while fructose, maltose, glucose and galactose were less higher in order. Also, adding organic acid reduced retention rate of the experimentals over the controls irrespective of sort and concentration of organic acid; also, the retention rate of the experiment with 0.5 M organic acid, the highest amount of all, reduced radically to 13.1 to 15.9% after 10 days storage. The retention rate of the experimentals added 1,000 ppm ascorbic acid and thio urea respectively after 10 days was 48.4%, which is higher than the controls by around 23.1%. As to amino acid, treatment on the pigment with aspartic acid 50 mM marked the highest retention rate of 41.5% and treatment with 2.0 M NaCl showed 31.6% retention rate, which was higher than the controls. While low temperature like $5^{\circ}C$ and $25^{\circ}C$ kept the stability of the pigment in storage, as temperature increased the stability of the pigment dropped rapidly.

A Bio-Inspired Cell-Microsystem to Manipulate and Detect Living Cells

  • Lim, Jung-Min;Byun, Sang-Won;Park, Tai-Hyun;Seo, Jong-Mo;Yoo, Young-Suk;Hum Chung;Dong-il
    • KIEE International Transactions on Electrophysics and Applications
    • /
    • v.4C no.4
    • /
    • pp.160-164
    • /
    • 2004
  • In this study, we demonstrate for the first time a bio-inspired Cell-Microsystem to manipulate and detect living cells. Cultured retinal pigment epithelial cell line (ARPE-19) was directed to grow in a pre-defined Cell-Microsystem. The three-dimensional micropillars of 5 ${\mu}{\textrm}{m}$ in height and diameter of the Cell-Microsystem were fabricated. Inhibited DNA synthesis and transformed cell morphology were observed throughout the culture period. The demonstration of manipulating and detecting living cells by the surface topography is a new approach, and it will be very useful for the future design of cell-based biosensors and bioactuators.

Graphiumins I and J, New Thiodiketopiperazines from the Marine-derived Fungus Graphium sp. OPMF00224

  • Fukuda, Takashi;Nagai, Kenichiro;Kurihara, Yuko;Kanamoto, Akihiko;Tomoda, Hiroshi
    • Natural Product Sciences
    • /
    • v.21 no.4
    • /
    • pp.255-260
    • /
    • 2015
  • Two new thiodiketopiperazines (TDKPs), designated graphiumins I (1) and J (2), were isolated from the culture broth of the marine-derived fungus Graphium sp. OPMF00224 by solvent extraction, silica gel column chromatography, and HPLC. Their absolute structures were elucidated by spectroscopic analyses (1D and 2D NMR data, ROESY correlations, and CD data) and chemical methods. They were found to be structurally rare TDKPs with a phenylalanine-derived indolin substructure. Compounds 1 and 2 inhibited yellow pigment production by methicillin-resistant Staphylococcus aureus (MRSA) with $IC_{50}$ values of 63.5 and $76.5{\mu}g/ml$, respectively, without inhibiting its growth, even at $250{\mu}g/ml$.

Red to Red - the Marine Bacterium Hahella chejuensis and its Product Prodigiosin for Mitigation of Harmful Algal Blooms

  • Kim, Doc-Kyu;Kim, Ji-Hyun F.;Yim, Joung-Han;Kwon, Soon-Kyeong;Lee, Choong-Hwan;Lee, Hong-Kum
    • Journal of Microbiology and Biotechnology
    • /
    • v.18 no.10
    • /
    • pp.1621-1629
    • /
    • 2008
  • Harmful algal blooms (HABs), commonly called red tides, are caused by some toxic phytoplanktons, and have made massive economic losses as well as marine environmental disturbances. As an effective and environment-friendly strategy to control HAB outbreaks, biological methods using marine bacteria capable of killing the harmful algae or algicidal extracellular compounds from them have been given attention. A new member of the $\gamma$-Proteobacteria, Hahella chejuensis KCTC 2396, was originally isolated from the Korean seashore for its ability to secrete industrially useful polysaccharides, and was characterized to produce a red pigment. This pigment later was identified as an alkaloid compound, prodigiosin. During the past several decades, prodigiosin has been extensively studied for its medical potential as immunosuppressants and antitumor agents, owing to its antibiotic and cytotoxic activities. The lytic activity of this marvelous molecule against Cochlodinium polykrikoides cells at very low concentrations ($\sim$l ppb) was serendipitously detected, making H. chejuensis a strong candidate among the biological agents for HAB control. This review provides a brief overview of algicidal marine bacteria and their products, and describes in detail the algicidal characteristics, biosynthetic process, and genetic regulation of prodigiosin as a model among the compounds active against red-tide organisms from the biochemical and genetic viewpoints.

Optimal Culture Conditions for Production of Yellow Pigments from Monascus purpureus in Liquid Culture (액체 배양에 의한 Monascus purpureus가 생산하는 황색색소의 최적 생산조건)

  • Choi Chung-Sig;Jeon Chun-Pyo;Lee Jung-Bok;Lee Oh-Seuk;Rhee Chang-Ho;Kwon Gi-Seok
    • Food Science and Preservation
    • /
    • v.13 no.2
    • /
    • pp.192-197
    • /
    • 2006
  • The optimum cultural conditions for production of yellow pigment by Monascus purpureus MMK2 were investigated in liquid culture. Monascus purpureus MMK2 was shown to give the maximum production of yellow pigment in the medium containing of 3.0% wheat flour, 0.15% $NaNO_3$, 0.25% $Na_2HPO_4\;12H_2O\;and\;0.15%\;MgSO_4\;7H_2O$. The optimal culture conditions for temperature and initial pH were $30^{\circ}C$ and 6.5, respectively. The yellow pigment production reached a maximum level at the 7th day of cultivation.