• 산업식품공학
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• 제21권3호
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• pp.233-241
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• 2017

Scaffolds of cell substrates are biophysical platforms for cell attachment, proliferation, and differentiation. They ultimately play a leading-edge role in the regeneration of tissues. Recent studies have shown the potential of bioactive scaffolds (i.e., osteo-inductive) through 3D printing. In this study, rice bran-derived biocomposite was fabricated for fused deposition modeling (FDM)-based 3D printing as a potential bone-graft analogue. Rice bran by-product was blended with poly caprolactone (PCL), a synthetic commercial biodegradable polymer. An extruder with extrusion process molding was adopted to manufacture the newly blended "green material." Processing conditions affected the performance of these blends. Bio-filament composite was characterized using field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Mechanical characterization of bio-filament composite was carried out to determine stress-strain and compressive strength. Biological behaviors of bio-filament composites were also investigated by assessing cell cytotoxicity and water contact angle. EDX results of bio-filament composites indicated the presence of organic compounds. These bio-filament composites were found to have higher tensile strength than conventional PCL filament. They exhibited positive response in cytotoxicity. Biological analysis revealed better compatibility of r-PCL with rice bran. Such rice bran blended bio-filament composite was found to have higher elongation and strength compared to control PCL.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.108-108
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• 2017

In this study, biocomposite filaments with agricultural by-products can be used in extrusion-based 3D (Three-dimensional) printing. Extrusion-based 3D printing stands as a promising technique owing to its versatility. We hypothesized that bio-filament composite consisted of something derived from agricultural by-products could be used as 3D printing materials that could overcome the drawbacks of PCL (poly-caprolactone). Bio-filament mixed with PCL and agricultural by-products was defined as r-PCL in this study. In order to find it out the optimal mixing ratio of filaments, we had investigated PCL, r-PCL 10%, r-PCL 20%, r-PCL 50% separately. The morphological and chemical characteristics of the filaments were analyzed by FE-SEM (Field emission scanning electron microscope) and EDX (Energy-dispersive X-Ray spectroscopy), and the mechanical properties were evaluated by stress-strain curve, water contact angle, and cytotoxicity analysis. Results of this study have been shown as a promising way to produce eco-friendly bio-filaments composite for FDM (Fused deposition modeling) method based 3D printing technology. Thus, we could establish biomimetic scaffolds based on bio-printer filaments mixed with agricultural by-product.

• Journal of the Korean Wood Science and Technology
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• 제46권1호
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• pp.107-113
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• 2018

본 연구에서는 대표적인 친환경 소재인 바이오복합재료(bio-composite)를 이용한 3D 프린터 필라멘트를 제작하였다. 바이오복합재료의 제조를 위해 매트릭스로는 생분해성 고분자인 poly lactic acid (PLA)를 그리고 충전제로는 대나무 분말(Bamboo flour)을 사용하였다. 대나무는 담양에서 생산되는 왕대, 솜대, 죽순대를 이용하였으며, 대나무 분말과 PLA의 혼합비율은 중량기준 10/90, 20/80, 30/70으로 설정하였다. 3개 죽종으로 제조한 대나무/PLA 바이오복합재료의 기본물성 평가를 위해 인장강도를 비교하였다. 그 결과, 왕대 분말/PLA의 비율이 10/90일 때의 인장강도가 7.12 MPa로 가장 높게 나타남으로써 3D 프린터 필라멘트 제작용 대나무/PLA 바이오복합재료로 가장 적합한 것으로 판단되었으며, 현미경 관찰 결과, 죽분의 함량을 더욱 낮춘 필라멘트를 제작할 필요성이 있다고 판단된다.

• Membrane and Water Treatment
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• 제15권1호
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• pp.11-19
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• 2024

This study presents the characteristics of growth and morphology of Anabaena sp., a representative filamentous cyanobacterium, depending on temperature variation from 10 to 30 ℃. Both the filament density (or number) and its length of Anabaena were highly affected by temperature, as well as growth stage. Rapid growth at a higher temperature led to an increase in Anabaena filament density, as well as optical density at 680 nm (OD₆₈₀). However, the number of vegetative cells within a single filament of Anabaena grown at 30 ℃ was smaller than those grown at lower temperatures, due to the intercalary division of the filament. Of the three different cells comprising a single Anabaena filament, the vegetative cell marginally affects the growth of Anabaena. The main dimensions of the vegetative cell, i.e., length and width, depend on the temperature and growth stage. The length-to-width (L/W) ratios of vegetative cells and akinetes were relatively consistent regardless of the temperature. However, in vegetative cells with dichotomous growth, the L/W ratio shows clear differences depending on their growth stage. It has been demonstrated that the L/W ratio could be used as an indicator to indirectly predict the growth stage of on-sit Anabaena samples.

• International Journal of Industrial Entomology and Biomaterials
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• 제30권2호
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• pp.75-80
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• 2015

The wet spinning of silk solution has attracted researchers' attention because of 1) unique properties of silk as a biomedical material and 2) easy control of the structure and properties of the regenerated silk fiber. Recently, studies have reported that different silkworm varieties produce silk with differences in the molecular weight (MW) and other mechanical properties of the regenerated silk fibroin (SF) film. In this study, we look at the effect of different Bombyx mori varieties on the wet spinning of SF. Although five regenerated SFs from different silkworm varieties have different MWs and solution viscosity, the wet spinnability and post drawing performance of regenerated SFs were not different. This result is due to low variability in the MW of the regenerated SF samples from the different silkworm varieties. In addition, unlike regenerated SF films, the mechanical properties of wet spun regenerated SF filament were not affected by silkworm variety. This result suggests that the mechanical properties of wet spun SF filament are less affected by MW than those of SF film are.

• 한국정밀공학회지
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• 제23권5호
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• pp.190-196
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• 2006

Transparent materials are widely used in the fields of optic parts and bio industry. We have experiment to find out the characteristics of the micromachining inside transparent materials using femtosecond laser pulses. With its non-linear effects by very high peak intensity, filament (plasma channel) was formed by the cause of the self-focusing and the self-defocusing. Physical damage could be found when the intensity is high enough to give rise to the thermal stress or evaporation. At the vicinity of the power which makes the visible damage or modification, the structural modification occurs with the slow scanning speed. According to the polarization direction to the scanning direction, the filament quality is quite different. There is a good quality when the polarization direction is parallel to the scanning direction. For fine filament, we could suggest the conditions of the high numerical aperture lens, the short shift of focusing point, the low scanning speed and the low power below 20 mW. As the examples of optics parts, we fabricated the fresnel zone plate with the $225{\mu}m$ diameter and Y-bend optical wave guide with the $5{\mu}m$ width.

• The Plant Pathology Journal
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• 제29권1호
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• pp.17-30
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• 2013

Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treat-ments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2010년도 제39회 하계학술대회 초록집
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• pp.293-293
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• 2010

Carbon nanotubes (CNTs) have attracted considerable attention as possible routes to device miniaturization due to their excellent mechanical, thermal, and electronic properties. These properties show great potential for devices such as field emission displays, CNT based transistors, and bio-sensors. The metals such as nickel, cobalt, gold, iron, platinum, and palladium are used as the catalysts for the CNT growth. In this study, diamond-like carbon (DLC) was used for CNT growth as a nonmetallic catalyst layer. DLC films were deposited by a radio frequency (RF) plasma-enhanced chemical vapor deposition (RF-PECVD) method with a mixture of methane and hydrogen gases. CNTs were synthesized by a hot filament plasma-enhanced chemical vapor deposition (HF-PECVD) method with ammonia (NH3) as a pretreatment gas and acetylene (C2H2) as a carbon source gas. The grown CNTs and the pretreated DLC filmswere observed using field emission scanning electron microscopy (FE-SEM) measurement, and the structure of the grown CNTs was analyzed by high resolution transmission scanning electron microscopy (HR-TEM). Also, using energy dispersive spectroscopy (EDS) measurement, we confirmed that only the carbon component remained on the substrate.

• Journal of Species Research
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• 제9권2호
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• pp.174-179
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• 2020

Larvae and pupae of Simulium (Eusimulium) satsumense were collected and recorded from Korea for the first time. Detailed descriptions and photographs of both immatures are provided. Currently, 42 species are known from the subgenus Eusimulium, which is found globally, mainly in the Holarctic Region, but unknown from Korea. Members of the subgenus Eusimulium are morphologically homogeneous in male, female, and pupa, making them extremely difficult to distinguish from one another. S. (E.) satsumense can be distinguished from other Korean black flies by the following combination of the characteristics: Pupa: Gill of four slender filaments, dorsalmost filament strongly divergent from other filaments; cocoon slipper shaped without anterodorsal projection; Larva: Head spots distinctly positive; postgenal cleft short, apically rounded or squared; antenna with proximal segment brown; abdominal segments IX with prominent ventral tubercles; and rectal papillae of three simple lobes. In addition to descriptions for larvae and pupae of the species, a key to genera and subgenera of Simulium of Korean black flies are also provided.

• 한국패류학회지
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• 제30권1호
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• pp.51-56
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• 2014

주사전자현미경과 광학현미경을 이용하여 북방전복 아가미의 형태와 조직화학적 특징에 대하여 기재하였다. 북방전복의 아가미는 원새형의 bipectinate형 이었다. 아가미 새엽의 표면은 잘 발달된 섬모들로 덮여있었다. 새엽의 상피층은 공통적으로 단층으로 원주형의 상피세포와 점액세포로 구성되어 있었다. AB-PAS (pH 2.5와 pH 1.0) 반응과 AF-AB (pH 2.5) 반응 결과, 점액세포들은 주로 산성의 뮤코점액다당류를 함유하고 있었다.