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Temperature effects on the growth and morphology of Anabaena sp.: lab-scale investigation and onsite validation

  • Oh Kyung Choi (Bio Resource Center, Institute for Advanced Engineering) ;
  • Dong Hyuk Shin (Department of Environmental Engineering, Korea University) ;
  • Dandan Dong (Department of Environmental Engineering, Korea University) ;
  • Sung Kyu Maeng (Department of Civil and Environmental Engineering, Sejong University) ;
  • Jungsu Park (Department of Civil and Environmental Engineering, Hanbat National University) ;
  • Jae Woo Lee (Department of Environmental Engineering, Korea University)
  • Received : 2023.07.17
  • Accepted : 2024.03.05
  • Published : 2024.01.25
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Abstract

This study presents the characteristics of growth and morphology of Anabaena sp., a representative filamentous cyanobacterium, depending on temperature variation from 10 to 30 ℃. Both the filament density (or number) and its length of Anabaena were highly affected by temperature, as well as growth stage. Rapid growth at a higher temperature led to an increase in Anabaena filament density, as well as optical density at 680 nm (OD680). However, the number of vegetative cells within a single filament of Anabaena grown at 30 ℃ was smaller than those grown at lower temperatures, due to the intercalary division of the filament. Of the three different cells comprising a single Anabaena filament, the vegetative cell marginally affects the growth of Anabaena. The main dimensions of the vegetative cell, i.e., length and width, depend on the temperature and growth stage. The length-to-width (L/W) ratios of vegetative cells and akinetes were relatively consistent regardless of the temperature. However, in vegetative cells with dichotomous growth, the L/W ratio shows clear differences depending on their growth stage. It has been demonstrated that the L/W ratio could be used as an indicator to indirectly predict the growth stage of on-sit Anabaena samples.

Keywords

Acknowledgement

This research was supported by the Korea Environmental Industry & Technology Institute (KEITI) through the Aquatic Ecosystem Conservation Research Program, funded by Korea Ministry of Environment (MOE) [2020003030006].

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