• Molecules and Cells
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• 제42권1호
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• pp.56-66
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• 2019

Histidine triad nucleotide-binding protein (HINT) is a member of the histidine triad (HIT) superfamily, which has hydrolase activity owing to a histidine triad motif. The HIT superfamily can be divided to five classes with functions in galactose metabolism, DNA repair, and tumor suppression. HINTs are highly conserved from archaea to humans and function as tumor suppressors, translation regulators, and neuropathy inhibitors. Although the structures of HINT proteins from various species have been reported, limited structural information is available for fungal species. Here, to elucidate the structural features and functional diversity of HINTs, we determined the crystal structure of HINT from the pathogenic fungus Candida albicans (CaHINT) in complex with zinc ions at a resolution of $2.5{\AA}$. Based on structural comparisons, the monomer of CaHINT overlaid best with HINT protein from the protozoal species Leishmania major. Additionally, structural comparisons with human HINT revealed an additional helix at the C-terminus of CaHINT. Interestingly, the extended C-terminal helix interacted with the N-terminal loop (${\alpha}1-{\beta}1$) and with the ${\alpha}3$ helix, which appeared to stabilize the dimerization of CaHINT. In the C-terminal region, structural and sequence comparisons showed strong relationships among 19 diverse species from archea to humans, suggesting early separation in the course of evolution. Further studies are required to address the functional significance of variations in the C-terminal region. This structural analysis of CaHINT provided important insights into the molecular aspects of evolution within the HIT superfamily.

• Animal Bioscience
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• 제37권5호
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• pp.826-831
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• 2024

Objective: The major histocompatibility complex in chicken demonstrates a great range of variations within varities, breeds, populations and that can eventually influence their immuneresponses. The preset study was conducted to understand the major histocompatibility complex-B (MHC-B) variability in five major populations of Bangladesh native chicken: Aseel, Hilly, Junglefowl, Non-descript Deshi, and Naked Neck. Methods: These five major populations of Bangladesh native chicken were analyzed with a subset of 89 single nucleotide polymorphisms (SNPs) in the high-density MHC-B SNP panel and Kompetitive Allele-Specific polymerase chain reaction genotyping was applied. To explore haplotype diversity within these populations, the results were analyzed both manually and computationally using PHASE 2.1 program. The phylogenetic investigations were also performed using MrBayes program. Results: A total of 136 unique haplotypes were identified within these five Bangladesh chicken populations, and only one was shared (between Hilly and Naked Neck). Phylogenetic analysis showed no distinct haplotype clustering among the five populations, although they were shared in distinct clades; notably, the first clade lacked Naked Neck haplotypes. Conclusion: The present study discovered a set of unique MHC-B haplotypes in Bangladesh chickens that could possibly cause varied immune reponses. However, further investigations are required to evaluate their relationships with global chicken populations.

• 한국초지조사료학회지
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• 제38권2호
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• pp.135-139
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• 2018

본 연구는 제주조릿대의 사초생산성과 사료가치평가를 진행하여 조릿대의 활용도를 높임과 동시에 현재 한라산에서 발생하고 있는 조릿대로 인한 식물종 다양성 감소문제를 완화하는데 도움이 되고자 실시하였다. 조릿대의 사초생산성은 실험결과 5월과 6월기간에 비교적 우수한 재생력을 보였으나, 예취가 반복 될수록 재생능력이 감소하여 생산성이 떨어지는 경향을 나타내었다. 조릿대의 일반성분 분석결과 조단백질은 가을철 함량보다 봄철에서 비교적 높은 함량을 보였으며 6월에서 $8.64{\pm}0.20%$로 다른 기간에 비해 비교적 높은 함량을 나타내었으며 가장 낮은 NDF, ADF의 함량은 6월에서 각각 $77.90{\pm}0.25%$, $42.33{\pm}0.49%$로 나타나 섬유소 함량이 매우 높은 것으로 판단된다. 조릿대는 NDF와 ADF의 함량은 높으나, 볏짚에 비해 조단백질 함량은 비교적 높은 것으로 판단되며 가축의 특성에 맞추어 급여한다면 이용성이 있을 것으로 여겨진다. 조릿대를 사료원으로 이용하기 위해서는 섬유소 분해능력이 좋은 흑염소 같은 가축 또는, 조릿대에 대한 기호성이 우수하고, 소화율이 비교적 우수한 말에게 급여하는 것이 효율적일 것으로 여겨진다. 조릿대의 사료화를 위해서는 보다 다양한 축종을 대상으로 추가 연구가 진행되어야 할것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제24권1호
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• pp.26-35
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• 2014

Saccharothrix algeriensis NRRL B-24137 produces naturally different dithiolopyrrolone derivatives. The enzymatic activity of pyrrothine N-acyltransferase was determined to be responsible for the transfer of an acyl group from acyl-CoA to pyrrothine core. This activity was also reported to be responsible for the diversity of the dithiolopyrrolone derivatives. Based on this fact, nine dithiolopyrrolone derivatives were produced in vitro via the crude extract of Sa. algeriensis. Three of them have never been obtained before by natural fermentation: acetoacetyl-pyrrothine, hydroxybutyryl-pyrrothine, and dimethyl thiolutin (holomycin). Two acyltransferase activities, acetyltransferase and benzoyltransferase catalyzing the incorporation of linear and cyclic acyl groups to the pyrrothine core, respectively, were biochemically characterized in this crude extract. The first one is responsible for formation of acetyl-pyrrothine and the second for benzoyl-pyrrothine. Both enzymes were sensitive to temperature changes: For example, the loss of acetyltransferase and benzoyltransferase activity was 53% and 80% respectively after pre-incubation of crude extract for 60 min at $20^{\circ}C$. The two enzymes were more active in neutral and basal media (pH 7-10) than in the acidic one (pH 3-6). The optimum temperature and pH of acetyltransferase were $40^{\circ}C$ and 7, with a $K_m$ value of $7.9{\mu}M$ and a $V_{max}$ of $0.63{\mu}M/min$ when acetyl-CoA was used as limited substrate. Benzoyltransferase had a temperature and a pH optimum at $55^{\circ}C$and 9, a $K_m$ value of $14.7{\mu}M$, and a $V_{max}$ of $0.67{\mu}M/min$ when benzoyl-CoA was used as limited substrate.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.67-67
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• 2020

Though there is an on-going need for the diversity of ornamental plants for in-door environment, their growth and stress adaptability in comparatively low light intensity condition require further studies for implementation. Here investigates the growth and chlorophyll fluorescence parameters of Farfugium japonicum (L.) Kitam, Liriope muscari (Decne.) L.H.Bailey and Acorus gramineus Aiton under several light intensities which were based on common in-door environment. The growth measurement of the plants included the quantity, length, width and SPAD value of the leaves. Calculated values of Fm/Fo, Fv/Fm, Pi_Abs, ETo/RC and DIo/RC were used as the parameters of the chlorophyll fluorescence under 10, 50, 100 and 200 PPFD (μmol·m^-2·s^-1) light intensities. In-door plants group was put in a closed room allowing no other light sources for 10 weeks and the control group was put in glass-greenhouse for the same period. The overall in-door growth of L. muscari was not significantly different in all light intensities compared to the control group and even showed the higher SPAD values. Also, an increasing tendency of Pi_Abs value under 10 to 100 PPFD was observed implying that L.muscari could adapt well to in-door environment. Measurement of A. gramineus growth mostly showed the highest values in the control group especially in the number of the leaves. Nevertheless, chlorophyll fluorescence parameters showed no significant value difference between in-door and the control groups and thus, A. gramineus might have possibility of successful adaptation to in-door environment. F. japonicum showed deficient growth in plant height and leaf length compared to the control but, it seemed to be able to sustain ornamental value under in-door light intensities. Furthermore, Pi_Abs and DIo/RC values were increased under in-door light conditions suggesting potential adaptability of F. japonicum.

• Journal of Microbiology and Biotechnology
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• 제21권2호
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• pp.187-191
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• 2011

Microbial fuel cells (MFCs) were successfully enriched using sludge contaminated with Cr(VI) and their characteristics were investigated. After enrichment, the charge of the final 10 peaks was 0.51 C ${\pm}$ 1.16%, and the anodic electrode was found to be covered with a biofilm. The enriched MFCs removed 93% of 5 mg/l Cr(VI) and 61% of 25 mg/l Cr(VI). 16S rDNA DGGE profiles from the anodic electrode indicated that ${\beta}$-Proteobacteria, Actinobacteria, and Acinetobacter sp. dominated. This study is the first to report that electrochemically active and Cr(VI)-reducing bacteria could be enriched in the anode compartment of MFCs using Cr(VI)-containing sludge and demonstrates the Cr(VI) removal capability of such MFCs.

• 한국실내디자인학회논문집
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• 제14권5호
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• pp.193-204
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• 2005

Design took a radical turn in the 1980's, deconstructing the rigid ideology of the Modernism, often with great humor. Contemporary representable Avant-garde designers, Philippe Starck and Ron Arad brazenly borrowed from the 'historical elements' of previous styles and created bio-morphic forms with whimsical names of furniture. This study is proposed to understand the diversity of contemporary design, by comparing these two furniture designers. It also covers the common factors in contemporary furniture design, and analyzes their individual differences and similarities in design. It helps to plan the foundation of design methods for 21C, and predict and respond to the future as a reference point. Philippe Starck links Romantic Rationalism within Minimal style and Ron Arad links Deconstructivism within High-tech style but both of them pursue of Organic concept design in their furniture through the concept of humanism. They are able to establish a base of contemporary furniture design by adapting new conceptual form, and design process for the general public.

• Ocean and Polar Research
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• 제24권4호
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• pp.359-365
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• 2002

The qualitative and quantitative composition of the phytoplankton of the coastal waters off Vladivostok during the period 1991-1994 was studied. The following trends in the phytoplankton composition with decreasing distance from the source of eutrophication were revealed: 1) total density and bio-mass increased; 2) the density of the diatom Skeletonema costatum, which reflects a decrease in the Shannon-Weaver species diversity index during the summer microalgal bloom, increased significantly; and 3) the density of the non-diatom component of the phytoplankton increased.

• Animal Systematics, Evolution and Diversity
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• 제33권1호
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• pp.37-44
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• 2017

The present census on freshwater bryozoans in the Han River, Geum River and in lentic water bodies in Gangwon Province revealed three gymnolaemate species. Two of them-Hislopia prolixa Hirose and Mawatari, 2011 and Victorella pavida Saville Kent, 1870 were recorded for the first time from South Korea. Paludicella articulata (Ehrenberg, 1831), on the other hand, had already been documented from a few sites since 1941. The bryozoans, all belonging to the order of Ctenostomata, were found on a variety of substrates in freshwater (H. prolixa and P. articulata) and brackish habitats (V. pavida). Hislopia prolixa had recently been established as a new species and this is the first record for an occurrence outside of Japan, where it had been newly described from Lake Biwa and nearby satellite lakes.

• 한국환경과학회지
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• 제24권9호
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• pp.1211-1220
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• 2015

Soil is the most important factor in natural environment for bio-diversity. Urbanization and development of city devastate urban soil by the fraternization of green network and run off pollution. In these facts, preservation of soil is the main issue in maintain of quality urban environment. In order to handle this issue, the gold network that link fragment soil patches is considered in maintain quality soil. This study researched the infiltration Treebox design technique based on the Low Impact Development. This technique suggest reduction of impervious area of the soil due to urbanization. The main concept of this study is encourage more permeable surfaces in urban area by using a infiltration planter. The function of the planter is hold run off as much as possible from intensive rainfall, and utilizes it in drought season. Also, this planter provides fertile soil for organism habitat by keeping appropriate moisture supplying.