• 한국자기공명학회논문지
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• 제16권2호
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• pp.147-161
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• 2012

Human melanocortin-4 receptor (hMC4R) among MC-Rs, expressed in the brain, is in charge of the control on energy homeostasis and food intake. The structure and function of human MC4R have been studied to understand their essential function and roles. To investigate the structure and function, it is necessary to prepare sufficient amounts of proteins. However, their expression and purification is demanding and time-consuming due to their innate insoluble and toxic properties. The heterozygous mutations of hMC4R, exchange of Asp 90 to Asn located in second transmembrane, cause severe obesity in human. To obtain purified hMC4R wt-TM2 for structural studies, it was first over-expressed and purified by fast protein liquid chromatography (FPLC) and then solution NMR studies were performed to get high-resolution spectra. In here, we established optimized purification scheme to get more purified target peptide.

• 약학회지
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• 제48권3호
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• pp.213-217
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• 2004

For the determination of glutathione S-transferase activity, a new method was established by using HPLC system. Moreover, amount of enzyme for a optimum reaction was determined by a comparative study with a variety concentration of enzyme. Using a established method, activity of glutathione S-transferase that is alcohol metabolizing enzyme was investigated on the fruit of Hovenia dulcis Thunb. As the result of experiment, EtOH and $H_2O$ extracts of the fruit of Hovenia dulcis Thunb showed visible a synergistic effect of glutathione S-transferase activity. On a continuous experiment, EtOH and $H_2O$ extracts of the fruit of Hovenia dulcis Thunb showed alcohol decomposition activity on the in vivo test using rat. These results suggest that the fruit of Hovenia dulcis Thunb may be useful in the prevention of hangover.

• Asia Marketing Journal
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• 제6권3호
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• pp.157-171
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• 2004

이 사례는 KT&G가 환경변화에 적응하는 전략을 소개하는 것이다. KT&G의 주력제품인 담배는 이미 암의 발생원인으로 지목되어 사회적 운동으로 금연캠페인을 하고 있을 정도로 시장여건이 좋지 않다. 위협 수준이 아니라 위기 수준이라고 할 수 있다. 이러한 위기를 기회로 전환시키는 전략으로 KT&G는 크게 네 가지 방향을 모색하고 있다. 먼저, 사회적인 저항 속에서도 흡연자는 꾸준히 존재하는 점을 감안하여 철저한 세분화전략으로 신제품을 출시하고 있다. 둘째, 타 소비재와 마찬가지로 고품질의 담배를 개발하여 브랜드파우워를 높이는 전략을 구사하고 있다. 셋째, 담배가 바이오산업의 핵심이 될 수 있음을 감안하여 기업이미지를 바이오와 연결시키려 노력하고 있다. 끝으로, KT&G의 강점인 인삼을 활용하여 웰빙산업에 진출하려는 관련 다각화의 계획을 가지고 있다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 춘계학술대회
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• pp.499-501
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• 2021

4차 산업에 대한 꾸준한 투자와 개발로 IoT 기술이 보다 발전하게 되었고, 이에 따라 필요한 보안 플랫폼을 제공할 수 있는 플랫폼 기반의 블록체인 기술이 필요하게 되었다. 블록체인 기반의 IoT 어플리케이션인 BIoT는 향후 보다 다양한 분야에 적용되기 위하여 보다 정교한 보안성과 확장성 및 효율성을 제공하는 플랫폼 기술이 요구된다. 본 논문에서는 최근 발표되어 이슈가 되고 있는 스마트 컨트랙트 ETH 2.0을 분석하여 기존의 BIoT 개발에 요구되는 사항들을 비교하고 향후 다가오는 BIoT의 미래를 전망하여 본다.

• 한국가구학회지
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• 제23권3호
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• pp.298-304
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• 2012

Since 1990's, a flooring based on plywood has gotten customers' demand. Costs of raw material and production increased because of changed environment of industry. Tropical timber such as Red Meranti (Shorea acuminate) used for raw material of the floor has been depleting beside countries in South Eastern Asia changed species of afforestation. As a result, it gets hard to secure good quality of raw material for plywood. Moreover plywood price is increased suddenly after earthquake in Japan. Eucalyptus (Eucalyptus globulus) in china has been using for raw material as a countermeasure of changed environment of industry. In this study, possibility of using flooring consisted of Eucalyptus veneer as a core layer was checked by heat transfer experiments. Flooring consisted of Red Meranti was used for comparison. Two factors which impact on heat transfer are a type of density gradient and density difference between Long-grain veneer and Short-grain veneer. Red Meranti samples are 'M' type of density gradient on the other hand Eucalyptus samples are 'W' type of density gradient.

• 한국산업융합학회 논문집
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• 제25권2_2호
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• pp.309-314
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• 2022

The bonding strength [ISO22674] test was conducted by firing a dedicated ceramic powder on the surface of the dental titanium material treated with micro-arc oxidation. In the test group, an average result value of 34.34 MPa was obtained, and in the control group, a result value of 21.53 MPa was obtained. The bonding strength of the test group was higher than that of the control group by 12.81 MPa, resulting in a 37% improvement in durability of the dental artificial tooth ceramic restoration.

• Journal of Microbiology and Biotechnology
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• 제33권10호
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• pp.1361-1369
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• 2023

Corynebacterium glutamicum (C. glutamicum) has been considered a very important and meaningful industrial microorganism for the production of amino acids worldwide. To produce amino acids, cells require nicotinamide adenine dinucleotide phosphate (NADPH), which is a biological reducing agent. The pentose phosphate pathway (PPP) can supply NADPH in cells via the 6-phosphogluconate dehydrogenase (6PGD) enzyme, which is an oxidoreductase that converts 6-phosphogluconate (6PG) to ribulose 5-phosphate (Ru5P), to produce NADPH. In this study, we identified the crystal structure of 6PGD_apo and 6PGD_NADP from C. glutamicum ATCC 13032 (Cg6PGD) and reported our biological research based on this structure. We identified the substrate binding site and co-factor binding site of Cg6PGD, which are crucial for understanding this enzyme. Based on the findings of our research, Cg6PGD is expected to be used as a NADPH resource in the food industry and as a drug target in the pharmaceutical industry.

• 식품보건융합연구
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• 제9권5호
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• pp.7-11
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• 2023

Purpose: The purpose of this study is to analyze current trends in the Korean ice cream industry and determine how these trends affect the competitiveness of each ice cream company. Method: This study was conducted by reviewing existing related literature and collecting and analyzing data from news articles, corporate reports, and existing researchers' studies related to the ice cream industry. Results: The Korean ice cream industry is growing and developing through the collaboration of fun marketing with taste, quality, and fun elements, and the young MZ generation can be said to be the main customer base. In terms of quality, premium ice cream made with high-quality domestic ingredients has recently been gaining popularity, and ice cream with reduced fat and sugar, such as plant-based substitutes, is also gaining popularity among consumers. Conclusions and Implications: The results of this study highlight the need for continued innovation in the Korean ice cream industry. The industry must also capitalize on the growth of online sales and the global appeal of Korean pop culture. Sustainability efforts, technology adoption, and strategic collaboration are key drivers for future industry success, and social media engagement must be integrated into marketing strategies to increase brand awareness and consumer loyalty.

• Environmental Engineering Research
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• 제25권1호
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• pp.18-28
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• 2020

In this paper the authors provide comparative evaluation of current research that used liquefaction and pyrolysis method for bio-oil production from various types of biomass. This paper review the resources of biomass, composition of biomass, properties of bio-oil from various biomass and also the utilizations of bio-oil in industry. The primary objective of this review article is to gather all recent data about production of bio-oil by using liquefaction and pyrolysis method and their yield and properties from different types of biomass from previous research. Shortage of fossil fuels as well as environmental concern has encouraged governments to focus on renewable energy resources. Biomass is regarded as an alternative to replace fossil fuels. There are several thermo-chemical conversion processes used to transform biomass into useful products, however in this review article the focus has been made on liquefaction and pyrolysis method because the liquid obtained which is known as bio-oil is the main interest in this review article. Bio-oil contains hundreds of chemical compound mainly phenol groups which make it suitable to be used as a replacement for fossil fuels.

• 생명과학회지
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• 제28권2호
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• pp.216-222
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• 2018

Desmodium heterocarpon은 콩과에 속하는 덩굴식물로 주로 한국, 일본 등의 아시아 국가에 분포되어 있다. 본 연구에서는 식물에 존재하는 신규 기능성 소재 개발의 일환으로 Desmodium heterocarpon 에탄올 추출물(DHEE)의 항산화 및 항염증 생리활성을 DPPH 라디칼 소거능, ROS 소거능, NO 생성 억제능 및 관련 단백질 발현을 통해 분석하였다. 먼저 DHEE의 항산화능을 DPPH 라디칼 소거능을 통해 분석한 결과 높은 소거활성을 보여 DHEE가 매우 강한 항산화능을 보유함을 확인하였다. 또한 RAW 264.7 세포주에서 H2O2에 의해 유도된 ROS에 대한 DHEE의 소거능을 분석한 결과 농도의존적인 강한 ROS 소거능을 보였다. 뿐만 아니라 대표적인 항산화 효소 중 하나로 항산화능 보유 천연물에 의해 발현이 유도되는 HO-1 및 그 전사 인자인 Nrf2의 단백질 발현이 DHEE의 처리에 의해 유의적으로 증가됨을 보였다. 한편 DHEE가 LPS에 의해 유도된 NO 생성에 미치는 영향을 분석한 결과 농도 의존적인 NO 생성 저해능을 보였으며 이는 NO 생성 단백질인 iNOS의 발현 저해에서 기인함을 확인하였다. 이러한 결과를 통해 DHEE의 높은 항산화능과 항염증 활성을 확인하였으며 향후 잠재적인 기능성 소재로서 유용하게 활용될 수 있을 것으로 판단된다. 추후 계속적인 연구를 통해 활성 물질의 규명이 필요할 것으로 보인다.