• Title/Summary/Keyword: Bio Chip

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Peeling Device of Chonggag Radish for Kimchi Processing (김치용 총각무 삭피장치)

  • Min, Y.B.;Kim, S.T.;Chung, T.S.;Moon, S.D.;Moon, S.W.
    • Journal of Biosystems Engineering
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    • v.31 no.5 s.118
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    • pp.403-409
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    • 2006
  • This study was performed to design a peeling device for root crop that were considered of the three possible types such as blade belt type, brush type, and rotating blade type, and to inspect the characteristics of the experimented peeling methods based on the chonggag (altari) radish tests. The peeling performances by the blade belt type and brush type devices were not established so well with the troublesome like adhesion of the chip on the blades was occurred, and the difficulties to produce the blade parts were come out on these types of the peeling devices. But the peeling operations by the rotating blade type peeling device was completed without needs to clean blades, and it was concluded that it would be possible to practical mechanization of the root crop which shaped similar chonggag radish for it's constructional simplicity and feasibility.

Development of serodiagnostic surface plasmon resonance imaging assay for the detection of antibodies to porcine circovirus type 2

  • Park, Chul;Kim, Bum-Seok;Kim, Yong-Hwan;Cho, Ho-Seong
    • Korean Journal of Veterinary Service
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    • v.34 no.1
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    • pp.1-4
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    • 2011
  • A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P<0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection.

Construction and Analysis of a DNA Microarray for the Screening of Biosynthetic Genes of Secondary-Metabolites formation in Streptomyces (방선균 유래 이차대사 생합성 유전자 분석용 DNA Microarray 제작 및 해석)

  • Nam Soo Jung;Kang Dae-Kyung;Rhee Ki Hyeong;Kim Jong-Hee;Kang Sang Sun;Chang Yong Keun;Hong Soon-Kwang
    • Korean Journal of Microbiology
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    • v.41 no.2
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    • pp.105-111
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    • 2005
  • Streptomyces produces many kinds of secondary-metabolites including antibiotics. Screening of a new compound and elucidation of a biosynthetic pathway for the secondary metabolites are very important fields of biology, however, there is a main problem that most of the identified compounds are already researched compounds. To solve these problems, a microarray system that is based on the data related to the biosynthetic genes for secondary-metabolites was designed. For the main contents of DNA microarray, the important genes for the bio-synthesis of aminoglycosides, polyenes group, enediyne group, alpha-glucosidase inhibitors, glycopeptide group, and orthosomycin group were chosen. A DNA microarray with 69 genes that were involved in the bio-synthesis for the antibiotics mentioned above was prepared. The usability of the DNA microarray was confirmed with the chromosomal DNA and total RNA extracted from S. coelicolor whose genomic sequence had already been reported.

Selection of the Superior Potato Clones Based on Acrylamide Reduction for Cold Chipping (아크릴아마이드 저감화된 콜드칩 가공용 우수감자 계통 선발)

  • Jin, Cheng Wu;Hwang, Won Nam;Cho, Dong Ha;Kang, Wie Soo;Lim, Hak Tae
    • Horticultural Science & Technology
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    • v.30 no.5
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    • pp.603-612
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    • 2012
  • In order to select potato clones for making cold chip, this study analyzed the glucose content, acrylamide content, and the correlation between the two properties after harvest, $4^{\circ}C$ and $8^{\circ}C$ low-temperature storage, and $20^{\circ}C$ heating treatment of 47 breeding clones and control cultivars 'Atlantic', 'Sumi', and 'Gui Valley'. In all of the control cultivars and 47 clones, glucose content was below 0.25% and acrylamide content was below 1000 ppb just after harvest, but after $4^{\circ}C$ low-temperature storage both the glucose content and acrylamide content increased rapidly and only 4 clones H7, H13, H16, and H40 showed a level below 500 ppb. In $8^{\circ}C$ low-temperature storage as well both contents increased, but the increase was relatively smaller than that in $4^{\circ}C$ low-temperature storage. In addition, $20^{\circ}C$ heating treatment decreased both contents. In the results of analyzing the correlation between glucose content and acrylamide content at low-temperature storage, a positive correlation was observed. In conclusion, clones H7, H13, H16, and H40 showing low glucose content even at low-temperature treatment were found to contain less acrylamide and therefore they were selected as potato clones suitable for making cold chip.

Anew formula CPC22 regulates bone loss, hot flashes, and dysregulated lipid metabolism in ovariectomized postmenopausal mice

  • Hee-Yun Kim;Hyunwoo Jee;Hosong Cho;Dongjun Park;Hyun-Ja Jeong
    • CELLMED
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    • v.13 no.14
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    • pp.15.1-15.15
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    • 2023
  • Background and objective: A new formular CPC22 consists of Cynanchum wilfordii root, Pueraria thomsonii flower, and Citrus unshiu peel and has been developed to improve the postmenopausal symptoms. The research intended to evaluate whether CPC22 would regulate bone loss, hot flashes, and dysregulated lipid metabolism in ovariectomized (OVX) postmenopausal mice. Method: The OVX mice were orally administered with CPC22 daily for 7 weeks. Results: CPC22 regulated OVX-induced bon loss by enhancing serum osteoprotegerin, alkaline phosphatase, and osteocalcin levels and diminishing serum receptor-activator of the NF-κB ligand (RANKL), collagen type 1 cross-linked N-telopeptide, and tartrate-resistant acid phosphatase levels. As a result of CPC22 treatment, notable decreases in tail skin temperature and rectal temperature were observed, along with diminishment in hypothalamic RANKL and monoamine oxidase A levels and enhancement in hypothalamic serotonin (5-HT), norepinephrine, dopamine, 5-HT2A, and estrogen receptor-β levels. CPC22 enhanced levels of serum estrogen and diminished levels of serum follicle-stimulating hormone and luteinizing hormone. CPC22 regulated levels of serum lipid metabolites, including total cholesterol, triglycerides, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol. Furthermore, CPC22 diminished levels of serum blood urea nitrogen, creatine kinase, alanine transaminase, aspartate aminotransferase, and lactate dehydrogenase and restored vaginal dryness without affecting uterus atrophy index and vagina weights. Conclusion: Therefore, these results indicated that CPC22 improves OVX-induced bone loss, hot flashes, and dysregulated lipid metabolism by compensating for estrogen deficiency without side effects, suggesting that CPC22 may be used for the prevention and treatment of post menopause.

A Compact Ka-Band Doppler Radar Sensor for Remote Human Vital Signal Detection

  • Han, Janghoon;Kim, Jeong-Geun;Hong, Songcheol
    • Journal of electromagnetic engineering and science
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    • v.12 no.4
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    • pp.234-239
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    • 2012
  • This paper presents a compact K-band Doppler radar sensor for human vital signal detection that uses a radar configuration with only single coupler. The proposed radar front-end configuration can reduce the chip size and the additional RF power loss. The radar front-end IC is composed of a Lange coupler, VCO, and single balanced mixer. The oscillation frequency of the VCO is from 27.3 to 27.8 GHz. The phase noise of the VCO is -91.2 dBc/Hz at a 1 MHz offset frequency, and the output power is -4.8 dBm. The conversion gain of the mixer is about 11 dB. The chip size is $0.89{\times}1.47mm^2$. The compact Ka-band Doppler radar system was developed in order to demonstrate remote human vital signal detection. The radar system consists of a Ka-band Doppler radar module with a $2{\times}2$ patch array antenna, baseband signal conditioning block, DAQ system, and signal processing program. The front-end module size is $2.5{\times}2.5cm^2$. The proposed radar sensor can properly capture a human heartbeat and respiration rate at the distance of 50 cm.