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Construction and Analysis of a DNA Microarray for the Screening of Biosynthetic Genes of Secondary-Metabolites formation in Streptomyces  

Nam Soo Jung (Department of Biological Science, Myongji University)
Kang Dae-Kyung (Bio-Resources Institute, Easy Bio System Inc.)
Rhee Ki Hyeong (College of Industrial Science, Kongju National University)
Kim Jong-Hee (Department of Biological Science, Myongji University)
Kang Sang Sun (Division of Science Education, Chungbuk National University)
Chang Yong Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
Hong Soon-Kwang (Department of Biological Science, Myongji University)
Publication Information
Korean Journal of Microbiology / v.41, no.2, 2005 , pp. 105-111 More about this Journal
Abstract
Streptomyces produces many kinds of secondary-metabolites including antibiotics. Screening of a new compound and elucidation of a biosynthetic pathway for the secondary metabolites are very important fields of biology, however, there is a main problem that most of the identified compounds are already researched compounds. To solve these problems, a microarray system that is based on the data related to the biosynthetic genes for secondary-metabolites was designed. For the main contents of DNA microarray, the important genes for the bio-synthesis of aminoglycosides, polyenes group, enediyne group, alpha-glucosidase inhibitors, glycopeptide group, and orthosomycin group were chosen. A DNA microarray with 69 genes that were involved in the bio-synthesis for the antibiotics mentioned above was prepared. The usability of the DNA microarray was confirmed with the chromosomal DNA and total RNA extracted from S. coelicolor whose genomic sequence had already been reported.
Keywords
Streptomyces; DNA microarray; S. coelicolor;
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