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http://dx.doi.org/10.7853/kjvs.2011.34.1.001

Development of serodiagnostic surface plasmon resonance imaging assay for the detection of antibodies to porcine circovirus type 2  

Park, Chul (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Kim, Bum-Seok (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Kim, Yong-Hwan (Department of Veterinary Research, Gwangju Metropolitan City Institute of Health & Environment)
Cho, Ho-Seong (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.34, no.1, 2011 , pp. 1-4 More about this Journal
Abstract
A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P<0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection.
Keywords
Porcine circovirus type 2; Surface plasmon resonance imaging; Protein chip;
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