• Journal of Microbiology
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• 제42권2호
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• pp.152-155
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• 2004

Pseudomonas sp. K82 has been reported to be an aniline-assimilating soil bacterium. However, this strain can use not only aniline as a sole carbon and energy source, but can also utilize benzoate, p-hydroxybenzoate, and aniline analogues. The strain accomplishes this metabolic diversity by using dif-ferent aerobic pathways. Pseudomonas sp. K82, when cultured in p-hydroxybenzoate, showed extradiol cleavage activity of protocatechuate. In accordance with those findings, our study attempted the puri-fication of protocatechuate 4,5-dioxygenase (PCD 4,5). However the purified PCD 4,5 was found to be very unstable during purification. After Q-sepharose chromatography was performed, the crude enzyme activity was augmented by a factor of approximately 4.7. From the Q-sepharose fraction which exhibited PCD 4,5 activity, two subunits of PCD4,5 (${\alpha}$ subunit and ${\beta}$ subunit) were identified using the N-terminal amino acid sequences of 15 amino acid residues. These subunits were found to have more than 90％ sequence homology with PmdA and PmdB of Comamonas testosteroni. The molecular weight of the native enzyme was estimated to be approximately 54 kDa, suggesting that PCD4,5 exists as a het-erodimer (${\alpha}$$_1$${\beta}$$_1$). PCD 4,5 exhibits stringent substrate specificity for protocatechuate and its optimal activity occurs at pH 9 and 15 $^{\circ}C$. PCR amplification of these two subunits of PCD4,5 revealed that the ${\alpha}$ subunit and ${\beta}$ subunit occurred in tandem. Our results suggest that Pseudomonas sp. K82 induced PCD 4,5 for the purpose of p-hydroxybenzoate degradation.

• 한국환경과학회지
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• 제8권1호
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• pp.7-18
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• 1999

밀양강 수계 7개 지점에 대하여 1997년 4월부터 12월까지 5회에 걸쳐 채집된 저서성 대형무척추동물의 군집분석 및 저서성 대형무척추동물에 의한 생물학적 수질평가 결과를 요약하면 다음과 같다. 분류군은 총 3문 8강 15목 37과 45속 81종으로서 빈모류 1종, 거머리류 2종, 복족류 6종, 부족류 5종, 갑각류 2종, 곤충류 65종으로 곤충류가 가장 많았다. 전체 지점에 대한 평균 개체수 현존량은 815개체/㎡였다. 이 중 곤충류(약80%)가 가장 많았으며, 다시 곤충류 중에는 하루살이류(약 70%)와 날도래류(약 18%)가 주를 이루었다. 각 지점별 평균개체수 현존량을 보면, 제1지점인 청도천의 경우 262개체/㎡(32.2%)로서 가장 많은 출현율을 보였고, 제7지점인 밀양강 끝지점의 경우 38개체 /㎡(4.7%)로서 가장 출현율이 낮았다. 전체 평균 수준에서의 제1우점종은 Ecdyomurus levis Navas였으며, 우점도지수는 0.3이었다. 각 지점별 우점종을 보면 제1지점부터 제5지점까지는 Ecdyomurus levis Navas이였고, 제6지점과 제7지점은 Hirudinidae sp.1이었다. 그리고, 전체 평균 수준에서의 다양도지수는 2.66이었다. 조사지점별 평균 다양도 지수를 보면 제4지점이 가장 높았으며(H'=3.47), 다음은 제2지점, 1지점, 3지점, 5지점, 6지점, 7지점의 순이었다. 생물학적 지표종을 중심으로한 수질평가에 의하면, 밀양강 전체의 평균은 군오염지수(GPI) 1,49로서 ${\beta}$-mesosaprobic의 II급수로 평가되었다. 각 지점별로는 제3지점의 경우 Oligosaprobic의 I급수로서 가장 양호한 수질이었고, 제1지점과, 제2지점, 제4지점, 제5지점은 ${\beta}$-mesosaprobic의 II급수로, 제6지점은 ${\beta}$-mesosaprobic의 III급수로 평가되었다. 그리고, 제7지점은 ${\alpha}$-mesosaprobic의 III급수로서 가장 수질이 불량한 것으로 평가되었다.

• 미생물학회지
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• 제38권3호
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• pp.192-197
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• 2002

러시아 바이칼호에서 해빙기에 부유세균과 aggregates에 부착한 세균의 군집구조를 FISH (fluorescent in situ hybridization)방법으로 0 m부터 250 m수심에서 비교 분석하였다. 조사대상은 Eubacteria에 속하는 세균과 class Proteobacteria에 속하는 세균 중 $\alpha$-, $\beta$-, $\gamma$ -group과 Cytophaga-Flavobacterium group,그리고 Planctomycetales였다. 부유세균의 수는 $0.2{\times}10^6\cells{\cdot}ml^-1 to 3.2{\times}10^6 \cells{\cdot}ml^-1$범위였으며, 수심이 깊어질수록 감소하였다. Aggregate에 부착한 세균은 부유세균과 반대로 수심이 깊어질수록 증가하였고, 개체수의 범위는 0.4~$3.3{\times}10^4$ $cells{\cdot}ml^-1$ 이였다. 총세균수에 대한 Eubacteria 수의 비율은 부유세균의 경우 52.3~74.1%, aggregates에 부착한 세균은 39.6~66.7%로 부유세균보다 부착세균에서 그 비율이 낮았으며, 세균의 군집구조 분석 결과에서도 부유세균과 aggregates애 부착한 세균의 군집구조가 다른 양상으로 나타났다. 특히 두 세균의 군집구조는 식물플랑크톤이 밀집해 있는 25 m 수심에서 급격히 변화하여 식물플랑크톤이 부유세균과 부착세균의 군집의 변화에 밀접한 관련이 있는 것으로 확인되었다, Aggregates에 부착한 세균 군집은 수심에 따라 매우 특이한 변화 양상을 나타내었다. $\beta$-proteobacteria group은 수심이 깊어지면서 그 비율이 높아져, 100m에서는 $\beta$-group이 총세균수는 51.8%를 차지하였으나, 250m에서는 $\gamma$-group이 43.8%를 차지하여, 급격하게 우점 group이 변화하였다. 그러나, 부유세균에서는 전혀 다른 군집 구조를 이루고 있었다. 이러한 결과에서 aggregates에 부착한 세균은 부유세균과는 다른 다양성을 이루고, 다른 천이과정을 거치는 것으로 확인되었다.

• Journal of Microbiology and Biotechnology
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• 제21권2호
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• pp.187-191
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• 2011

Microbial fuel cells (MFCs) were successfully enriched using sludge contaminated with Cr(VI) and their characteristics were investigated. After enrichment, the charge of the final 10 peaks was 0.51 C ${\pm}$ 1.16%, and the anodic electrode was found to be covered with a biofilm. The enriched MFCs removed 93% of 5 mg/l Cr(VI) and 61% of 25 mg/l Cr(VI). 16S rDNA DGGE profiles from the anodic electrode indicated that ${\beta}$-Proteobacteria, Actinobacteria, and Acinetobacter sp. dominated. This study is the first to report that electrochemically active and Cr(VI)-reducing bacteria could be enriched in the anode compartment of MFCs using Cr(VI)-containing sludge and demonstrates the Cr(VI) removal capability of such MFCs.

• Journal of Microbiology
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• 제45권1호
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• pp.64-68
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• 2007

In this study we examined the multi-drug resistance profiles of the tetracycline (TC) resistant genus Vibrio to determine its susceptibility to two ${\beta}-lactams$, ampicillin (ABPC), and mecillinam (MPC), as well as to macrolide, erythromycin (EM). The results showed various patterns of resistance among strains that were isolated from very close geographical areas during the same year, suggesting diverse patterns of drug resistance in environmental bacteria from this area. In addition, the cross-resistance patterns suggested that the resistance determinants among Vibrio spp. are acquired differently within the sediment and seawater environments.

• Mycobiology
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• 제34권4호
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• pp.159-165
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• 2006

One of the most economically viable processes for the bioconversion of many lignocellulosic waste is represented by white rot fungi. Phanerochaete chrysosporium is one of the important commercially cultivated fungi which exhibit varying abilities to utilize different lignocellulosic as growth substrate. Examination of the lignocellulolytic enzyme profiles of the two organisms Phanerochaete chrysosporium and Rhizopus stolonifer show this diversity to be reflected in qualitative variation in the major enzymatic determinants (ie cellulase, xylanase, ligninase and etc) required for substrate bioconversion. For example P. chrysosporium which is cultivated on highly lignified substrates such as wood (or) sawdust, produces two extracellular enzymes which have associated with lignin deploymerization. (Mn peroxidase and lignin peroxidase). Conversely Rhizopus stolonifer which prefers high cellulose and low lignin containg substrates produce a family of cellulolytic enzymes including at least cellobiohydrolases and ${\beta}-glucosidases$, but very low level of recognized lignin degrading enzymes.

• Mycobiology
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• 제39권4호
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• pp.266-271
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• 2011

Species of Phoma and its allies were isolated during a survey on the diversity of endophytic fungi associated with pine trees in Korea. Based on the phylogenetic analyses of internal transcribed spacer and ${\beta}$-tubulin gene sequences, two Phoma-like species from the needles of Pinus koraiensis were identified as Peyronellaea calorpreferens and P. glomerata. They were also morphologically identified based on the previous descriptions. Here, we report P. calorpreferens and P. glomerata being present in Korea as endophytic fungi in Pinus koraiensis.

• Mycobiology
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• 제44권4호
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• pp.248-259
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• 2016

During fungal diversity surveys of the order Eurotiales in Korea, two fungal strains, EML-DG33-1 and EML-NCP50, were isolated from samples of rat dung and fig tree leaf collected at a garden located in Gwangju in 2014. To complete the National Species List of Korea, it is a prerequisite to verify whether many questionable species, which were previously recorded but not confirmed, indeed present in Korea. Herein, the isolates were confirmed as undescribed species, Paecilomyces variotii and Talaromyces amestolkiae based on the combination of morphological and phylogenetic analyses of multigenes including the rDNA internal transcribed spacer, ${\beta}-tubulin$, and RNA polymerase II subunit 2.

• Mycobiology
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• 제49권2호
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• pp.105-121
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• 2021

Six interesting fungal strains were isolated during a survey of fungal diversity associated with freshwater; these strains were designated as CNUFC YJW2-22, CNUFC MSW11-6-2, CNUFC HRS5-3, CNUFC MSW242-6, CNUFC DMW2-2, and CNUFC CPWS-1. Based on a polyphasic approach including phylogenetic analyses of internal transcribed space (ITS), large subunit (LSU), beta-tubulin (BenA), and calmodulin (CaM) gene sequences, morphological analyses, the six strains were found to be identical to Acremonium guillematii, Cadophora novi-eboraci, Lectera nordwiniana, Mycoarthris corallina, Talaromyces siamensis, and Tetracladium globosum, respectively. To our knowledge, these are the first records of the rare Lectera, Mycoarthris, and Tetracladium genera in Korea, and the first reports of A. guillematii, C. novi-eboraci, L. nordwiniana, M. corallina, T. siamensis, and Te. globosum in a freshwater environment.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2023년도 가을학술발표대회논문집
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• pp.237-238
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• 2023

Urbanization and the increase in building scale have amplified the complexity of M.E.P design. Traditional design methods face limitations when considering intricate pathways and variables, leading to an emergent need for research in automated design. Initial algorithmic approaches encountered challenges in addressing complex architectural structures and the diversity of M.E.P types. However, with the launch of OpenAI's ChatGPT-3.5 beta version in 2022, new opportunities in the automated design sector were unlocked. ChatGPT, based on the Large Language Model (LLM), has the capability to deeply comprehend the logical structures and meanings within training data. This study analyzed the potential application and latent value of LLMs in M.E.P design. Ultimately, the implementation of LLM in M.E.P design will make genuine automated design feasible, which is anticipated to drive advancements across designs in the construction sector.