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Application of data fusion modeling for the prediction of auxin response elements in Zea mays for food security purposes

  • Nesrine Sghaier;Rayda Ben Ayed;Ahmed Rebai
    • Genomics & Informatics
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    • v.20 no.4
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    • pp.45.1-45.7
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    • 2022
  • Food security will be affected by climate change worldwide, particularly in the developing world, where the most important food products originate from plants. Plants are often exposed to environmental stresses that may affect their growth, development, yield, and food quality. Auxin is a hormone that plays a critical role in improving plants' tolerance of environmental conditions. Auxin controls the expression of many stress-responsive genes in plants by interacting with specific cis-regulatory elements called auxin-responsive elements (AuxREs). In this work, we performed an in silico prediction of AuxREs in promoters of five auxin-responsive genes in Zea mays. We applied a data fusion approach based on the combined use of Dempster-Shafer evidence theory and fuzzy sets. Auxin has a direct impact on cell membrane proteins. The short-term auxin response may be represented by the regulation of transmembrane gene expression. The detection of an AuxRE in the promoter of prolyl oligopeptidase (POP) in Z. mays and the 3-fold overexpression of this gene under auxin treatment for 30 min indicated the role of POP in maize auxin response. POP is regulated by auxin to perform stress adaptation. In addition, the detection of two AuxRE TGTCTC motifs in the upstream sequence of the bx1 gene suggests that bx1 can be regulated by auxin. Auxin may also be involved in the regulation of dehydration-responsive element-binding and some members of the protein kinase superfamily.

'Mansu', a Hardy Kiwifruit (Actinidia arguta Planch. et Miq.) Cultivar with Improved Storage Life

  • Jung, Byung Joon;Cho, Hye Sung;Park, Moon Young;Cho, Youn Sup
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.755-760
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    • 2016
  • In general, the storage life of hardy kiwifruit (Actinidia arguta) is approximately one month, even in cold storage, which is a limiting factor for its commercialization due to the short marketing period. This short shelf life is in contrast to those of green kiwifruit (A. deliciosa, 6 to 8 months) and gold kiwifruit (A. chinensis, 4 to 6 months). To increase the storage life of hardy kiwifruit, we performed a cross between A. arguta (a local collection, 'Hy2-1', female) and A. deliciosa ('Matua', male) at Wando station at the Fruit Research Institute of JARES, Korea in 1999. After the first selection in 2006, we clonally propagated the hybrid plants by grafting them onto A. arguta seedlings in Gwangyang and Haenam province, Korea. We performed the final selection of this cultivar in 2012 and applied for plant protection rights from the Korea Forest Research Institute in 2013. Several experimental orchards have been established for commercial production. 'Mansu' begins to flower on May 20 in Gwangyang. The horticultural maturity date in 'Mansu', when the soluble solid content reaches $7^{\circ}Bx$, is October 15, whereas that of the control variety ('Chiak') is October 5. The average fruit size of 'Mansu' is approximately 15 g. The soluble solid content of 'Mansu' is approximately 16 to $17^{\circ}Bx$ after ripening. The total yield of mature 'Mansu' vines is estimated to be 2.2 to 2.5 tons per 10a. The fruit firmness of 'Mansu' exceeded 1.5 kg/5 mmØ until 70 days after storage (at 0 and $1^{\circ}C$). Therefore, 'Mansu' fruits have much longer storability than the control. The cultivation and production of 'Mansu' may extend the marketing period for hardy kiwifruit.

Oviposition Time of Overwintered Females and Migration of Crawlers of Pseudaulacaspis prunicola (Homoptera: Diaspididae) on Cherry Trees in Jeju Island (제주도 벚나무에 발생하는 벚나무깍지벌레 월동성충의 산란시기 및 부화약충 이동시기)

  • Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.44 no.3 s.140
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    • pp.231-235
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    • 2005
  • This study was conducted to obtain the optimal spray time for Pseudaulacaspis prunicota (Maskell) (Homoptera: Diaspididae) in early seaon in Jeju. Oviposition time of overwintered females and activity of hatched nymphs of P. prunicola were monitored, and the phenology data were compared with the outputs estimated by a degree-days model of P. pentagona (Targioni-Tozzetti)). Overwintered females of P. prunicola began to lay eggs from mid to late April, and the eggs started to hatch from early May followed by the active migration of the hatched nymphs during mid May. The phenological events of P. prunicola in early season were likely comparable with those of P. pentagona reported in southern Korea and in central Japan. A degree-day model, which predicts the proportion of >50% hatched egg batches of P. pentagona (y=1[exp(-(-a+bx))]; y, proportion; x, degree-days based on $10.5^{\circ}C$ from 1 January; a=-18.80 and b=0.073), accurately described the migration time of P. prunicola hatched nymphs. Thus, it is considered that the degree-day model can be used for predicting the optimal spray time for P. prunicola in early season.

Analysis of Chemical Components of Korean Loquat (Eriobotrya japonica Lindl.) Fruit (국내산 비파 열매의 화학적 성분 분석)

  • Lee, Boo-Yong;Park, Eun-Mi;Kim, Eun-Jeong;Choi, Hee-Don;Kim, In-Hwan;Hwang, Jin-Bong
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.428-432
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    • 1996
  • The chemical components of Korean loquat (Eriobotrya japonica Lindl.) fruit were analysed. Approximate compositions of loquat flesh and seed were as follows. respectively crude lipid 0.53% and 0.83%, crude protein 0.05% and 5.27%, crude fiber 3.46% and 3.49%, crude ash 3.24% and 2.78%, carbohydrate 92.72% and 87.63% Soluble solids content, pH and acidity (citric acid) of loquat flesh juice were $12^{\circ}Bx$ by saccharometer, 4.43 and 0.18%, respectively. Free sugar compositions of loquat flesh and seed extracts $(3^{\circ}Bx)$ were as follows, respectively; fructose 0.77% and 0.31%, glucose 0.73% and 0.79%, sucrose 0.52% and 0.19%, ribose and 0.56%, Loquat flesh contained Glu 336.72 mg%, Asp 251.06 mg%, Arg 30.90 mg% and Lys 5.26 mg% Loquat seed contained Glu 448.23 mg%, Asp 335.63 mg%, lle 44.20 mg% and His 37.89 mg%, Potassium (k) contents of loquat flesh and seed were 32627.95 mg% and 28936.28 mg% in total amount of crude ash, while vitamin A and C of loquat flesh and seed were not detected. Composition of major lipid of loquat fruit seed oils fractionated by silicic acid was neutral lipids 43.78%, glycolipids 12.32% and phospholipids 43.90%, Fatty acid compositions of loquat seed lipid extracted by chloroform-methanol (2 : 1) were as follow; palmitic acid 23.72%, stearic acid 3.815, oleic acid 8.55%, linoleic acid 54.29% and linolenic acid 9.63%, Neutral lipids consist of palmitic acid 28.89, stearic acid 6.80%, oleic acid 11.07%, linoleic acid 40.67% and linolenic acid 12.58%, Glycolopids cinsist of palmitic acid 13.21%, stearic acid 4.56%, oleic acid 6.53%, linoleic acid 64.92% and linolenic aicd 10.77% Phospholipids consist of palmitic acid 30.95%, stearic acid 3.40%, oleic acid 9.09%, linoleic acid 48.45% and linolenic acid 8.10%.

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Biological Activities and Quality Characteristics of Rice Germ after Microbial Fermentation (미생물 발효 쌀 배아의 품질특성 및 생리활성)

  • Song, Hyo-Nam;Lee, Youn Ri
    • The Korean Journal of Food And Nutrition
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    • v.30 no.1
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    • pp.59-66
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    • 2017
  • This study investigated the quality characteristics and biological activities of rice germ fermented by Bacillus spp. During the milling process, the contents of rice germ in the rice bran (Control) were adjusted to 30% (RG30) and 70% (RG70). The approximate composition, pH, total acidity, total soluble solid, total sugar, polyphenol and flavonoid contents were measured. DPPH radical scavenging activity, xanthine oxidase (XO) and angiotensin converting enzyme (ACE) activities were also determined. We observed that the moisture content decreased after fermentation, while the crude protein was significantly increased. Fermentation remarkably lowered the pH from 5.83~6.26 to 4.77~4.93, thereby elevating the total acidity. Fermentation also increased the total solid contents, from $0.40{\sim}0.87^{\circ}Bx$ to $1.63{\sim}2.20^{\circ}Bx$. The total sugar decreased to 136.81~151.53 mg/mL from 377.56~450.64 mg/mL. Polyphenol contents were the highest in control (0.59 and 0.73 mg/mL before and after fermentation, respectively). Fermentation significantly affected the increase of the polyphenols in both rice germ 30% and 70% samples, from 0.26 and 0.28 mg GAE/g before fermentation, to 0.52 and 0.70 mg GAE/g after fermentation, respectively. There was a slight increase in the flavonoid contents after fermentation. The $IC_{50}$ value of the electron donating ability, as evaluated by the DPPH method, was the lowest in control (3.77 and 3.36 mg/mL before and after fermentation, respectively). Fermentation increased the XO inhibition activity up to 63.69% in control, 49.81% in rice germ 30%, and 59.32% in rice germ 70%. The ACE inhibition activities were also increased in the fermented control, rice germ 30% and 70%, to 40.51%, 22.69% and 33.91%, respectively.

Physicochemical Characteristics and Sensory Properties of Omija Wines Fermented by Active Dry Yeast Strains (활성건조효모를 이용한 오미자 발효주의 이화학 및 관능 특성)

  • Lee, Si-Hyung;Park, Hae-Kyung;Kim, Myung-Hee
    • Korean Journal of Food Science and Technology
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    • v.42 no.6
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    • pp.739-742
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    • 2010
  • In order to select a superior yeast, 8 kinds of commercial active dry yeasts (Lalvin 1116, Lalvin 1118, Lalvin D-47, Lalvin Bourgovin, Parisienne, Fermivin, Red Star Monrachet, and Red Star Premier Cuvee) were utilized for omija wine fermentation. During fermentation, the physicochemical characteristics and sensory properties of the various omija wines were evaluated. According to the results, pH and titratable acidity were in ranges of 3.0-3.3% and 1.8-2.4%, respectively. Sugar content was $24^{\circ}Bx$ at early fermentation and changed to $8.4-10.2^{\circ}Bx$ at 24 days of fermentation. While the omija wines fermented by Lalvin D-47 and Red Star Premier Cuvee showed the highest alcohol contents (13.0%), the omija wine fermented by Parisienne showed the lowest alcohol content (10.8%). The omija wine fermented by Lalvin 1118 had an alcohol content of 12.0% and showed the lowest yeast count of 5.8 log CFU/mL. Hunter's values (L, a, and b) were all different among the 8 omija wines. Moreover, the omija wine fermented by Lalvin 1118 showed the highest scores for taste ($6.75{\pm}1.68$), swallowing ($6.65{\pm}1.50$), and overall acceptability ($6.70{\pm}1.34$). It is concluded that Lalvin 1118 was the best yeast among 8 tested commercial active dry yeasts, having a high potential for omija wine fermentation.

Antioxidant Activity and Fermentation Characteristics of Blueberry Wine Using Traditional Yeast (토종효모를 이용한 블루베리 발효주의 발효 특성 및 항산화 활성)

  • Yoon, Hae Hoon;Chae, Kyu Seo;Son, Rak Ho;Jung, Ji Hye
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.6
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    • pp.840-846
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    • 2015
  • We investigated blueberry wine made using traditional yeast (Saccharomyces cerevisiae M-5) and Saccharomyces cerevisiae Fermivin which is widely used in wine manufacturing, and measured its fermentative characteristics and antioxidant activity. S. cerevisiae M-5 is a traditional yeast isolated from domestically grown Black raspberry (Rubus occidentalis). Both S. cerevisiae M-5 and Fermivin were inoculated into blueberry juice (BBJ) up to $1{\times}10^9CFU/kg$, followed by incubation at $25^{\circ}C$ for 39 days. Final fermentation products of blueberry fermented with S. cerevisiae M-5 (BBM) presented 13.10% alcohol, $8.42^{\circ}Bx$ of sugar, and 1.183% acidity, and final fermentation products of blueberry fermented with Fermivin (BBF) presented 14.20% alcohol, $8.2^{\circ}Bx$ of sugar, and 1.153% acidity. The contents of total polyphenol and flavonoid compounds of BBM were higher than those of BBF and BBJ. DPPH and ABTS radical scavenging activities and reducing power of BBM were higher than those of BBF. The sensory evaluation revealed that BBM showed excellent flavor, taste, and overall acceptability compared with BBF. Based on these results, the possibility of industrial utilization of S. cerevisiae M-5 as traditional yeast was confirmed by sensory evaluation and antioxidant activity. Fermentation rate of S. cerevisiae M-5 was similar to Fermivin, which is used in the food industry.

'Choha', an Early-maturing and High-quality Apricot (식미 우수한 조생종 살구 '초하(初夏)')

  • Kang, Sang-Jo;Chung, Kyeong-Ho;Kim, Ho-Yeol;Jun, Ji-Hyae;Lee, Don-Kyun
    • Horticultural Science & Technology
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    • v.17 no.1
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    • pp.21-22
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    • 1999
  • 'Choha' was released by the National Horticultural Research Institute in 1997 as a new early-maturing and high-quality apricot variety, which originated from the crossing of 'N.Y. 472' and 'Early Orange' in 1986. This variety was tested as 'Wonkyo Sa-05' at 4 areas for 3 years from 1995. 'Choha' is resistant to fruit cracking by rainy spells just before fruit maturing. Fruits mature in late June, earlier than any other varieties. The content of soluble solids is $12.5^oBx$ and fruit acidity is low. Fruits are relatively large, about 60g in weight.

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'Chosi', Early Maturing Sweet Persimmon (Diospyros kaki Thunb.) for 'Chuseok' (조생종 추석용 단감 '초시' 육성)

  • Ma, Kyeong-Bok;Cho, Kwang-Sik;Kang, Sam-Seok;Kim, Yoon-Kyeong;Hwang, Hae-Sung;Choi, Jang-Jeon;Han, Jeom-Hwa
    • Korean Journal of Breeding Science
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    • v.51 no.4
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    • pp.424-427
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    • 2019
  • A persimmon (Diospyros kaki L. Thunb.) cultivar, 'Chosi', which was a cross between 'Johongsi' and 'Nishimurawase' in 2000, was finally selected in 2010. 'Chosi' persimmon fruits were harvested in Sept. 19 in Naju, with a medium fruit size of 161 g, on average, and high soluble solids of approximately 15.3 °Bx. The flesh tastes good, with a crispy texture, and the juice is rich. The shape of the fruit is round oblate, and the skin color is orange; the appearance is graceful. Physiological disorders, such as stylar-end cracking or skin fine cracking, rarely occur in this cultivar. (Registration No. 7075)

The Effect of Vigor of a Bearing Shoot in ‘Bluecrop’ Highbush Blueberry (Vaccinium corymbosum L.) on Growth Characteristics of Shoots and Fruits (하이부시 블루베리 ‘블루크롭’의 결과지 세력이 신초와 과실 생장에 미치는 영향)

  • Kwon, Yeuseok;Lee, Sung-Hee;Shin, Hyunman;Nam, Sang-Yeong;Oh, Youngjae;Kim, Daeil
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.598-603
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    • 2016
  • The aim of this study was to elucidate the influence of vigor of a bearing shoot in ‘Bluecrop’ Highbush Blueberry (Vaccinium corymbosum L.) on growth characteristics of shoots and fruits. Bearing shoots were classified with BS (bearing shoot) and BMB (bearing mother branch). The vigor of bearing shoots were divided into four arbitrary categories; A was thin (< 6.0 ㎜) BMB and short (< 10 ㎝) BS, B was thin BMB and long (≥ 10 ㎝) BS, C was thick (≥ 6 ㎜) BMB and short BS and D was thick BMB and long BS. Shoots from D were longer (6.5 ㎝) and thicker (1.70 ㎜) than those from the others. Shoots of D had more leaves (5.8 ea) than those of the others. Leaf area of D was larger (13.5 ㎠) than those of the others. The first harvest of D was one week faster than the others. Ratio of big berry (>14 ㎜) from the long BSs was higher (B : 41.7, D : 46.8%) than that from the short BSs. Soluble solid content of small berrys did not show any different according to vigor of bearing shoots, but soluble solid content of big berrys of the long BSs was higher (B : 16.2, D : 15.6°Bx) than those of the short BSs. The thickness of BMB did not affect ratio of fruit size and soluble soild content. The long BSs would be proper than the short BSs for bearing bigger fruits.