• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.547-554
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• 2010

Low-molecular-weight glutenin subunit (LMW-GS) in common wheat (Triticum aestivum L.) is important for quality processing of bread and noodles. The objectives of this study were to clarify the composition of LMW-GSs and to identify their corresponding proteins. Using LMW-GS specific primers we cloned and characterized 43 LMW-GS genes in the wheat cultivar 'Jokyoung'. Some of these genes contain polypeptides different in size due to the presence of various deletions or insertions within repetitive and glutamine-rich domains. The comparison of deduced amino acid sequence of the LMW-GS genes in Jokyoung with that of 12 groups LMW-GSs of wheat cultivar Norin 61 showed that the deduced amino acid sequences were nearly the same to LMW-GS groups of 1, 2, 3/4, 5, 7, 10 and 11. All LMW-GS genes contain eight cysteine residues, which are conserved among all of the typical LMW-GS sequences. The relative positions of cysteine residues are also conserved, except those of the first and seventh. Based on phylogenetic analysis, the 43 sequences with the same N-terminal and C-terminal amino acid sequences were clustered in the same group. To identify the proteins containing the corresponding amino acid sequences, we determined the N-terminal amino acid sequence of 7 spots of LMW-GSs of Jokyoung separated by two-dimensional gel electrophoresis (2DE). Of them, Glu-B3 (LMW-m and LMW-s) and Glu-D3 (LMW-m) were detected in two and three spots, respectively and the others were not clear. Collectively, we classified diverse LMW-GSs and identified their corresponding protein products. These results will be helpful in breeding programs for improvement of wheat flour quality.

• Journal of Nutrition and Health
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• v.11 no.4
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• pp.11-23
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• 1978

This study is based on data from the nutritional and clinical survey in Chung Nam area, namely Myl-Yang-Ri, Yoon-Gok-Myon, Chyung Yang Koon. Under the direction of two Nutrition professors and two clinical pathology professors it was carried by 35 Dae Jeun medical Junior College students majoring in nutrition and clinical pathology from 18 July to 25 July 1978. The nutrition surveys were carried out with subjects in village from a total 67 households, 36 of them were randomly selected. The clinical surveys were carried out with subjects in village from a total 382 inhabitant, 154 inhabitant were randomly selected and 109 peoples were subjected to stool examination. The results obtained in this study are summarized as follows. 1) Nutritional survey a) Food Intake The average food intake per person per day in survey area was 929.9 grams (95.5% in vegetables and 4.5% in animal foods). The average consumption of the basicfood groups per person per day was 965g for meats and legumes (10.4% of the total food in take). 268.9g for fruits and vegetables (29% of the total food intake), 559.1g for cereals and potatoes(60.1% of the total food intake), 5.2g for milk and small fishes and 0.2g for fats and oils. b) Nutrient Intake The average daily consumption of calories and nutrients was 2054.1 kcal and 61.2g for total proteins, 223.2mg for calcium, 9.5mg for iron, 4914.4 IU for vitamin A, 1.5mg for thiamin, 1.2mg for riboflavin, 19.9mg for niacin and 54.7mg for ascorbic acid. When these figures are compared with the recommended allowances for Korean, the calories and nutrients intakes were insufficient. Especially the intakes of the calcium were lower than the recommended allowance which are 500 milligrams per day. c) Kinds of food stuffs consumed The kinds of food stuffs consumed by the subjects were 47 figures total. Generally these kinds of food were vegetables food. 2) Clinical Survey a) The mean value of hemoglobin from 72 males was 13.2g/dl, that of 82 females was 12.3g/dl. b) The proportion of low hemoglobin (<12.2g/dl for male and <11.3g/dl fo female) for male was 20.8%, females was 11.0% and from 24 male & female (both sexes) were 15.6% c) The mean value of Hematocrit of males was 39.6%, that of females was 37.4%. d) The mean value of MCHC of males was 33.9%, that of females was 33.6%. e) The ABO blood group was distributed as following; 30.5%, for group O, 29.2% for group A, 24.0% for group B, and 16.3% for AB group. Biochemical race index was 1.13. f) The over all prevalence rate of heminthic infectious by rate of each helminth was as following; Ascaris lumbricoides 33.9%, Thrichocephalus trichiurus 11.9%, Hookworm 0.9%, Hymenolepis diminuta 0.9%, Trichostromgylus orientalis 0.9% and the prevalence rate of two more helminthic infection was 7.2%.

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.195-203
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• 2012

The essential oil was extracted by steam distillation from the aerial part of erect bur-marigold (Bidens tripartita L.), one of the noxious weed in paddy field. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The fragrance of the essential oil was green, herbal, oily, spicy. There were 42 constituents in the essential oil：17 hydrocarbons, 6 alcohols, 6 acetates, 5 N-containing compounds, 3 ethers, 3 ketones, 1 lactone and 1 S-containing compound. Major constituents were ${\alpha}$-phellandrene (22.50%), ${\alpha}$-pinene (22.21%), 2,4-dimethyl (2,5-dimethylphenyl) methyl ester benzoic acid (15.11%), limonene (10.66%), ${\beta}$-pinene (35.43%), and ${\beta}$-cubebene (5.27%). The $IC_{50}$ value in MTT assay using HaCaT keratinocyte cell line was 0.018%. However, attachment of patch with 0.1% of the erect bur-marigold essential oil for 24 hr did not show any skin toxicity. Overall results of this study suggest that the essential oil of erect bur-marigold could be used as a source for the development of perfumery industrial products.

• The Korean Journal of Food And Nutrition
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• v.18 no.2
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• pp.140-145
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• 2005

Changes of plasma lipids, total cholesterol and phospholipids content according to diet of silkworm extract powder were as follows. Decrease ratios of plasma lipids at 3, $5\%(w/w)$ treating groups were plasma lipids 5.16, $9.15\%$, those of total cholesterols were 1.63, $2.76\%$ and those of phospholipids were 1.37, $2.00\%$, respectively. Decreasing effects of plasma triglyceride content according to diet of silkworm extract powder was higher than those of total cholesterols and phospholipids contents. Decrease of plasma glucose concentrations were $4.62\%\;and\;6.46\%\;at\;3\%,\;5\%(w/w)$ treating group than control group, respectively. These results were not proportional decrease according to treating amount. In the other hand, changes of insulin concentration of plasma, pancreas and femur were appeared the similar tendency with plasma glucose concentration. Relationship between treating amount of silkworm extract powder and insulin concentration had not positive relativity, In conclusion, treatment of silkworm extract powder about $3\%(w/w)$ could be expected to prevention and/or medical treatment effect of diabetic patients.

• Korean Journal of Radiology
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• v.20 no.1
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• pp.58-68
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• 2019

Objective: To compare digital breast tomosynthesis (DBT) and conventional full-field digital mammography (FFDM) in the detectability of breast cancers in patients with dense breast tissue, and to determine the influencing factors in the detection of breast cancers using the two techniques. Materials and Methods: Three blinded radiologists independently graded cancer detectability of 300 breast cancers (288 women with dense breasts) on DBT and conventional FFDM images, retrospectively. Hormone status, histologic grade, T stage, and breast cancer subtype were recorded to identify factors affecting cancer detectability. The Wilcoxon signed-rank test was used to compare cancer detectability by DBT and conventional FFDM. Fisher's exact tests were used to determine differences in cancer characteristics between detectability groups. Kruskal-Wallis tests were used to determine whether the detectability score differed according to cancer characteristics. Results: Forty breast cancers (13.3%) were detectable only with DBT; 191 (63.7%) breast cancers were detected with both FFDM and DBT, and 69 (23%) were not detected with either. Cancer detectability scores were significantly higher for DBT than for conventional FFDM (median score, 6; range, 0-6; p < 0.001). The DBT-only cancer group had more invasive lobular-type breast cancers (22.5%) than the other two groups (i.e., cancer detected on both types of image [both-detected group], 5.2%; cancer not detected on either type of image [both-non-detected group], 7.3%), and less detectability of ductal carcinoma in situ (5% vs. 16.8% [both-detected group] vs. 27.5% [both-non-detected group]). Low-grade cancers were more often detected in the DBT-only group than in the both-detected group (22.5% vs. 10%, p = 0.026). Human epidermal growth factor receptor-2 (HER-2)-negative cancers were more often detected in the DBT-only group than in the both-detected group (92.3% vs. 70.5%, p = 0.004). Cancers surrounded by mostly glandular tissue were detected less often in the DBT only group than in the both-non-detected group (10% vs. 31.9%, p = 0.016). DBT cancer detectability scores were significantly associated with cancer type (p = 0.012), histologic grade (p = 0.013), T and N stage (p = 0.001, p = 0.024), proportion of glandular tissue surrounding lesions (p = 0.013), and lesion type (p < 0.001). Conclusion: Invasive lobular, low-grade, or HER-2-negative cancer is more detectable with DBT than with conventional FFDM in patients with dense breasts, but cancers surrounded by mostly glandular tissue might be missed with both techniques.

• Korean Journal of Poultry Science
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• v.43 no.4
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• pp.207-212
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• 2016

This work was carried out to investigate the ability of thirteen crossbreds for production of commercial chicken (CC) of Korean native chicken to be cut out for Korean circumstance. Total of one thousand three hundred of crossbreds were divided thirteen groups (100 birds/group) that were twelve groups (1A, 1B, 2A, 2B, 3A, 3B, 4A, 4B, 5A, 5B, 6A, 6B) to crossbreed with two sires (A, B) and six dams (1, 2, 3, 4, 5, 6) of parent stock (PS) of Korean native chicken and one group (HH) as commercial Korean native chicken. Experimental diets were starter diet (0~7d; CP 22.0% ME 3,150 kcal/kg), early diet (8d~Wt 800 g; CP 19.0% ME 3,150 kcal/kg) and finished diet (Wt 800 g~12 wk; CP 18.0% ME 3,150 kcal/kg). Fertility of 1B group was higher than other groups. Hatchability per fertilization eggs and hatchability per setting eggs were 87.5~89% and 87.9~96.3%, respectively, and those of 4B and 6B groups were lower compared to other groups. Weekly body weight of HH group was lower than other groups. CV values of body weight of thirteen groups were showed over 12% as 12.5~18.2%, and especially, those of 2B, 3B, 5B, HH groups were lower compared to other groups. Weekly feed conversion ratios (FCRs) of 13 crossbreds were 1.7~2.3, 2.1~2.5, 2.4~2.7 and 2.7~3.2 at the age of 6, 8, 10 and 12 weeks, respectively. Weekly production indexes of 2A, 3A and HH groups were the lower than other groups and those of thirteen groups decreased following weeks. Feed costs per body weight gain (kg) were 1,069~1,255 won, 1,100~1,263 won, and 1.468~1,696 won at starter, earlier and finished period of broilers. These results leaded down to conclusion to be possible selection of new Korean native commercial chicken that have the excellent performance and economics.

• Journal of Oriental Neuropsychiatry
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• v.31 no.1
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• pp.1-12
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• 2020

Objectives: The purpose of this study was to evaluate the reliability and validity of the instrument on pattern identification for insomnia (PIT-Insomnia) and verify the correlation between PIT-Insomnia and psychological tests. Methods: Two evaluators examined the pattern identification of the participants who met insomnia disorder diagnostic criteria of the Diagnostic and Statistical Manual of Mental Disorder, Fifth Edition (DSM-5) and took the Insomnia Severity Index (ISI) score over 15 once manually and twice using the PIT-Insomnia to measure the inter-rater and test-retest reliability. We also conducted the following surveys: the Pittsburgh Sleep Quality Index (PSQI), the Korean version of Beck's depression inventory (K-BDI), the Korean version of the State-Trait Anxiety Inventory (STAI-K), the Korean Symptom checklist-95 (KSCL-95), and the EuroQol-5 dimension (EQ-5D), to measure concurrent validity and correlation between the PTI-Insomnia and psychological tests. Results: 1. The test-retest reliability analysis of the pattern identification results showed moderate agreement, and test-retest reliability analysis of each pattern identification score showed agreements from poor to moderate. 2. The inter-rater reliability analysis of the pattern identification results via manual showed slight agreement, when analysis was performed with calibration, the inter-rater reliability analysis of the pattern identification results via manual showed fair agreement. 3. The concordance analysis between results via manual and the PIT-Insomnia showed poor agreement, when the analysis was performed with calibration, concordance analysis showed fair agreement. 4. The concordance analysis between the PIT-Insomnia and the PSQI showed positive linear correlation. 5. The concordance analysis between the PIT-Insomnia and the PSQI, K-BDI, STAI-K, KSCL-95, and EQ-5D showed that non-interaction between the heart and kidney have positive linear correlation with the K-BDI, anxiety item of KSCL-95, dual deficiency of the heart-spleen have positive linear correlation with somatization item of KSCL-95, paranoia item of KSCL-95, heart deficiency with timidity have positive linear correlation with stress vulnerability item of KSCL-95, parania item of KSCL-95, phlegm-fire harassing the heart have positive linear correlation with K-BDI, paranoia item of KSCL-95, depressed liver qi transforming into fire have positive linear correlation with the anxiety item of KSCL-95, parania item of KSCL-95, all pattern identification have negative linear correlation with EQ-5D. Conclusions: The PIT-Insomnia has moderate agreement of reliability and reflects the severity of insomnia since it has some concurrent validity with the PSQI. There are some correlations between the PTI-Insomnia with specific psychological tests, so we could suggest it can be used appropriately in the clinical situation.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.586-593
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• 2014

Newly reclaimed tidal land is known to be in low status of soil fertility. The incorporation of crop residue is an effective method to improve soil properties and fertility in reclaimed saline soils. The objective of this study was to evaluate the efficiency of rice straw (RS) application to improve physico-chemical properties of saline-sodic soil and its contribution to productivity of whole crop barley. Increasing rate of rice straw improved growth parameter related to yield of whole crop barley, which increased tiller number significantly (p<0.05).The yield increased by 15% (F.W) and 9% (D.W) in rice straw-amended plots. The content of soil organic matter (SOM) in the surface soil (0-20cm) with rice straw incorporation increased by 5~9% (RS 2.5~RS 7.5) compared to RS 0, in which the content of SOM decreased after two consecutive cultivations. Rice straw incorporation promoted soil physico-chemical properties and nutrient-availability of the test crop, as indicated in change in soil bulk density, porosity and increased nutrient uptake of plant. Especially, the P content and uptake of whole crop barley increased with increasing the rate of rice straw application. In conclusion, the rice straw application at rates of $5.0-7.5ton{\cdot}ha^{-1}$ in reclaimed saline soils effectively improved soil properties and crop productivity, which has potentials to reduce the loss of chemical fertilizers and facilitate the favorable condition for crop growth under adverse soil condition.

• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.2
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• pp.180-183
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• 2010

This study was performed to investigate the temperature-dependent development, longevity and oviposition of an indigenous larval parasitoid, Meteorus pulchricornis, on tobacco cutworm, Spodoptera litura. M. pulchricornis were reared at nine constant temperatures between 15 and $35^{\circ}C$. The developmental times of each three developmental stage decreased from 38.7 to 16.3 d between 15 and $30^{\circ}C$. However, M. pulchricornis showed longer developmental time at $32.5^{\circ}C$ (9.5, 7.7 and 17.2 days for each three developmental stage) than at $30^{\circ}C$ (8.9, 7.3 and 16.3 days for each three developmental stage). Immature M. pulchricornis could not develop any more at $35^{\circ}C$. The lower developmental threshold estimated by linear regression equation for the egg to cocoon, cocoon to adult emergence and egg to adult emergence were 5.1, 4.6 and $4.5^{\circ}C$. The thermal constant for each of the three stages were 217.2, 176.2 and 403.8 degree-days, respectively. When no food or 50% honey solution as a food source is provided for M. pulchricornis, the parasitoid survived for 8.3 and 55.9 days at $25^{\circ}C$, respectively. M. pulchricornis females laid 5.2 eggs daily and total of 131.6 eggs at $25^{\circ}C$ until it died. Peak age-specific fecundity was observed on $14^{th}$ day (9.6 cocoons) after parasitoid emergence and gradually decreased thereafter.

• Journal of Plant Biotechnology
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• v.41 no.4
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• pp.216-222
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• 2014

This study was carried out to develop an efficient transformation protocol via particle bombardment with PLBs (protocorm-like bodies) in Phalaenopsis. To achieve this aim, osmoticum treatment and an increasing shooting chances in particle bombardment process were applied for this study. In addition, pCAMBIA3301: ORE7 vector which contains a herbicide-resistance bar gene as a selectable marker and ORE7 gene as a gene of interests were employed. With regard to the increasing chances of shooting in particle bombardment, double shooting was the best results with 1.5 ~ 2.5 times higher than those of a single or triple shooting treatment in the productioon of PPT (D-L-phosphinothricin)-resistant PLBs. However, regeneration rate of shoots in double shooting was not high as a single shooting. Further, double shooting showed 35 ~ 40% higher than that of a single shooting in the frequency of browning. Regarding effects of different osmotic treatments, combination of 0.2 M sorbitol with 0.2 M mannitol showed the best results in transformation efficiency, regeneration of transformants and reduction of browning. Putative transgenic Phalaenopsis plants were analyzed by PCR analysis and confirmed the presence of bar and ORE 7 gene. Also, real-time PCR was conducted by using 21 transgenic plants and showed only 4 plants had one copy of transgene; whereas, the other 17 plants had more than 2 copies of transgene. Transgenic phalaenopsis plants produced in this study were transferred to pots and flowered normally without morphological variations in flower and leaf.