• The Journal of Korean Medicine
• /
• v.40 no.4
• /
• pp.61-71
• /
• 2019

Objectives: The purpose of this study is to determine the effect of Yuhyangjeongtong-san on the recovery of tibial fractures in rats. Methods: In this study, osteocalcin and Calcitonin, CTX-2, TGF-β and BMP-2, which are used as indicators of bone formation, were analyzed after hematologic fractures using experimental rats. In addition, the fracture union process was confirmed using X-rays. Results: Osteocalcin, Calcitonin and BMP-2 showed a significant increase compared with the control at 4 weeks. CTX-2 and TGF-β showed a significant increase compared with the control at 3 weeks. On X-ray, YJS treated group, as the experiment progressed, the boundary line became blurred, the bone outline was clearly visible, and the fracture recover was progressing. Conclusion: The findings suggest that YJS can play a significant role in the repair of fractures. Therefore YJS is likely to be used to treat fractures.

• Journal of Soil and Groundwater Environment
• /
• v.21 no.2
• /
• pp.22-28
• /
• 2016

In order to evaluate landfill stabilization based on organic carbon, stoichiometric analysis and a biological methane potential (BMP) test based on modeling were performed at the 2^nd Sudokwon Landfill Site. Mass balance analysis through a BMP test proved to be more adaptable for evaluation, and it showed that 28.9% of landfill organic carbon was expected to remain by 2046, 30 years after landfill closure. The organic carbon ratio of total landfill waste for 2046 is forecasted as 2.9% in demolition waste and 5.1% in household waste, and, if one were to consider plastic as an organic waste, the ratios would increase to 15.9% and 28.3%, respectively. Therefore, it seems that organic matter biodegradation facilitating measures such as bioreactor landfill technology and preemptive recovery of combustible waste are necessary to shorten post closure management periods and to meet the landfill stabilization guidelines more safely.

• Korean Journal of Poultry Science
• /
• v.44 no.3
• /
• pp.211-223
• /
• 2017

Transforming growth factor beta ($TGF-{\beta}$) signaling pathways are involved in the regulation of proliferation, differentiation, immunity, survival, and apoptosis of many cells. The aim of this study was to investigate the differential expression of $TGF-{\beta}$-related genes, and their interactions and regulators in the spleen of two genetically disparate chicken lines (Marek's disease resistant line 6.3 and Marek's disease-susceptible line 7.2) induced with necrotic enteritis (NE) by Eimeria maxima and Clostridium perfringens infection. By using high-throughput RNA-sequencing, we investigated 76 $TGF-{\beta}$-related genes that were significantly and differentially expressed in the spleens of the chickens. Approximately 20 $TGF-{\beta}$ pathway genes were further verified by qRT-PCR, and the results were consistent with our RNA sequencing data. All 76 identified genes were analyzed through Gene Ontology and mapped onto the KEGG chicken $TGF-{\beta}$ pathway. Our results demonstrated that several key genes, including $TGF-{\beta}$1-3, bone morphogenetic proteins (BMP)1-7, inhibitor of differentiation (ID) proteins ID1-3, SMAD1-9, and Jun, showed a markedly differential expression between the two chicken lines, relative to their respective controls. We then further predicted 24 known miRNAs that targeted BMP7 mRNA from 139 known miRNAs in the two chicken lines. Among these, six miRNAs were measured by qRT-PCR. In conclusion, this study is the first to analyze most of the genes, interactions, and regulators of the $TGF-{\beta}$ pathway in the innate immune responses of NE afflicted chickens.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.9 no.2
• /
• pp.583-599
• /
• 2015

We apply the Bayesian matching pursuit (BMP) algorithm to the estimation of time-frequency selective channels in orthogonal frequency division multiplexing (OFDM) systems. By exploiting prior statistics and sparse characteristics of propagation channels, the Bayesian method provides a more accurate and efficient detection of the channel status information (CSI) than do conventional sparse channel estimation methods that are based on compressive sensing (CS) technologies. Using a reasonable approximation of the system model and a skillfully designed pilot arrangement, the proposed estimation scheme is able to address the Doppler-induced inter-carrier interference (ICI) with a relatively low complexity. Moreover, to further reduce the computational cost of the channel estimation, we make some modifications to the BMP algorithm. The modified algorithm can make good use of the group-sparse structure of doubly selective channels and thus reconstruct the CSI more efficiently than does the original BMP algorithm, which treats the sparse signals in the conventional manner and ignores the specific structure of their sparsity patterns. Numerical results demonstrate that the proposed Bayesian estimation has a good performance over rapidly time-varying channels.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.37
• /
• pp.4.1-4.6
• /
• 2015

Osteochondroma is rarely reported in the maxillofacial region; however, it is prevalent in the mandibular condyle. This slowly growing tumor may lead to malocclusion and facial asymmetry. A 39-year-old woman complained of gradual development of anterior and posterior unilateral crossbite, which resulted in facial asymmetry. A radiological study disclosed a large tumor mass on the top of the left mandibular condyle. This bony tumor was surgically removed through condylectomy and the remaining condyle head was secured. Subsequently, bimaxillary orthognathic surgery was performed to correct facial asymmetry and malocclusion. Pathological diagnosis was osteochondroma; immunohistochemistry showed that the tumor exhibited a conspicuous expression of BMP-4 and BMP-2 but rarely expression of PCNA. There was no recurrence at least for 1 year after the operation. Patient's functional and esthetic rehabilitation was uneventful.

• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.6
• /
• pp.1049-1057
• /
• 2012

The study investigated the biochemical methane potential (BMP) assay of pig slurry supplemented with mixed methanogens and cellulolytic bacteria to improve anaerobic digestion for methane production. For the BMP assay, 7 different microbial supplementation groups consisted of the cultures of mixed methanogens (M), Fibrobacter succinogenes (FS), Ruminococcus flavefaciensn (RF), R. albus (RA), RA+FS, M+RA+FS, and control. The cultures were added in the batch reactors with the increasing dose levels of 1% (0.5 mL), 3% (1.5 mL) and 5% (2.5 mL). Incubation for the BMP assay was carried out for 60 days at $38^{\circ}C$ using anaerobic digestate obtained from an anaerobic digester with pig slurry as inoculum. In results, 5% RF and RA+FS increased total biogas up to 8.1 and 8.4%, respectively, compared with that of control (p<0.05). All 5% microbial culture supplements significantly increased methane production up to 12.1~17.9% compared with that of control (p<0.05). Total solid (TS) and volatile solid (VS) digestion efficiencies showed no relationship to the increased supplementation levels of microbial cultures. After incubation, pH values in all treatment groups ranged between 7.527 and 7.657 indicating that methanogensis was not inhibited during the incubation. In conclusion, the results indicated that both hydrolysis and methanogenesis stages for methane production in anaerobic batch reactors were influenced by the supplemented microorganisms due to the chemical characteristics of pig slurry, but only the 5% supplementation level of all microbial culture supplements used in the experiment affected methane production.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.9 no.3
• /
• pp.154-159
• /
• 2006

It is essential to understand the decomposition characteristics for developing the optimum anaerobic digestion system of organic wastes. In this study, BMP (Biochemical Methane Potential) test using serum bottle was conducted to evaluate the anaerobic degradability of fish offal. 3 different groups of fish offal including waste from mackerel and hairtail handling except viscera and fish viscera were chosen for the substrates. Grinded fish offal was transferred anaerobically to serum bottle in amounts of 50 ml, 100 ml and 150 ml, respectively. BMP test was carried out in triplicate. Cumulative methane production and methane production rate depending on incubation time were evaluated. These results varied depending on substrate characteristics. The average values of ultimate methane yield ranged between $420ml{\cdot}CH_4/g{\cdot}VS$ and $490ml{\cdot}CH_4/g{\cdot}VS$, and the methane production and degradation rate of viscera were higher than those of other parts of fish offal. According to the analysis of elemental composition, average C/N ratio of fish offal used in this study was 5.2. Theoretical ultimate methane yield calculated from elemental composition was $522ml{\cdot}CH_4/g{\cdot}VS$. Biodegradability was calculated as 0.847.

• Journal of Korean Society on Water Environment
• /
• v.26 no.3
• /
• pp.432-438
• /
• 2010

LIDMOD2 was developed for evaluation of low impact development (LID) and best management practice (BMP) by modification of Site Evaluation Tool (SET). The modification includes employment of SCS-CN method for annual runoff simulation, unit load method for annual pollutant loads simulation, and the method proposed by Korean TMDL for calculating pollutant reduction by BMPs. The CN values were updated with regionalized parameters within Nack-Dong River basin because these are important parameters for simulating hydrology. LIDMOD2 was tested by applying to Andong Bus terminal. As a simulation results, pollutant loads and surface runoff will be significantly increased by post-development without LID compared with those from pre-development. LID technique was simulated to efficiently reduce surface runoff and pollutant load and increase infiltration. LIDMOD2 is screening level tool and easy to use because LIDMOD2 is based on spread sheet and most of parameters are regionalized. LIDMOD2 was illustrate that it could evaluate LID well by summarizing and graphing annual hydrology, annual pollutant loading, and hydrograph for event storm. The calculation methods related with pollutant loads are employed from the guideline of Korean TMDL and it can be useful tool for Korean TMDL to evaluate the effect of LID/BMP on developing area.

• The Korea Journal of Herbology
• /
• v.37 no.1
• /
• pp.51-59
• /
• 2022

Objectives : The process through which mesenchymal cells condense and differentiate into chondrocytes to form new bone is known as endochondral bone formation. Chondrogenic differentiation and hypertrophy are essential steps in bone formation and are influenced by various factors. The stem bark and root bark of Eleutherococcus sessiliflorus (ES) have been widely used to treat growth retardation and arthritis in traditional Korean Medicine. In this study, we aimed to investigate the possible role of the stem bark of ES in the stimulation of chondrogenic differentiation in clonal murine chondrogenic ATDC5 cells. Methods : In ATDC5 cells treated with ES extract, cell viability and extracellular matrix production were determined using CCK-8 assay and Alcian blue staining, respectively, and alkaline phosphatase activity was measured. We also examined mRNA and protein expression levels of genes related to chondrogenic expression in ATDC5 cells using reverse transcription-polymerase chain reaction and western blot analyses. Results : ES extract increased the accumulation of Alcian blue-stained cartilage nodules and alkaline phosphatase activity in ATDC5 cells. It increased the mRNA expressions of chondrogenic markers including bone sialoprotein (BSP), cartilage collagens, Runt-related transcription factor-2 (RUNX-2), osteocalcin (OCN), β-catenin, and bone morphogenetic protein-2 (BMP-2), as well as the protein expressions of β-catenin, RUNX-2, BMP-2, and alkaline phosphatase (ALP). Conclusion : Taken together, these results suggest that ES extract exhibits a chondromodulating activity and therefore may be a possible agent for the treatment of bone growth disorders.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.33 no.1
• /
• pp.28-39
• /
• 2007

The purpose of this study is to evaluate the regenerative capacity of reconstruction in the atrophied posterior maxilla by comparing bone graft procedures and alveolar distraction osteogenesis (ADO) techniques. We performed the autogenous iliac bone graft (AGB group, 5 specimens in 3 patients), and the combination (Mixed group, 3 specimens in 3 patients) of the autogenous and deproteinized bovine bone ($Bio-Oss^{(R)}$, Geistlich Co., Switzerland) as the ratio of 2:1 in the sinus floor elevation procedures. ADO procedures using $TRACK^{(R)}$ (KLS Martin Co., Germany) were also performed to augment vertical alveolar height in atrophied posterior maxilla (ADO group, 5 specimens in 4 patients). Newly generated bone tissues were obtained with the 2.0mm diameter trephine bur (3i Co., USA) during implant fixture installation after 5-7 months. Routine histolomorphological observation, immunodot blot assay for quantitative evaluation, and immunohistochemical staining with antibodies to MMP-1, -9, -10, TIMP-1, -2, and BMP-2, -4 were all carried out. Lamellar bone formation was well shown in all specimens and new bone formations of ADO group increased than those of other procedures. In immunohistochemical staining, the strong expression of BMP-2 was shown in all specimens, and immunodot blot assay showed that bone formation is accompanied by the good induction of factors associated with angiogenesis and appeared more increased amount of osteogenic and angiogenic factors in ADO group. ADO is the most effective technique for new bone formation compared to sinus floor elevation with autogenous or mixed bone graft in the atrophied posterior maxilla. In the quantitative immunodot blot assay, the regenerated bone after ADO showed more increased products of VEGF, BMP-2, PCNA and MMP-1 than those after the other procedures, and these findings were able to be confirmed by immunohistochemical stainings.