The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Eleutherococcus sessiliflorus induces differentiation of prechondrogenic ATDC5 Cells

오가피(Eleutherococcus sessiliflorus)의 전연골성 ATDC5 세포의 분화 유도

  • Shrestha, Saroj Kumar (School of Dentistry, Jeonbuk National University) ;
  • Song, Jungbin (Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Lee, Sung Hyun (NEUMED R&BD Institute) ;
  • Lee, Donghun (Department of Herbal Pharmacology, College of Korean Medicine, Gachon University) ;
  • Kim, Hocheol (Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Soh, Yunjo (School of Pharmacy, Jeonbuk National University)
  • Received : 2021.12.30
  • Accepted : 2022.01.25
  • Published : 2022.01.30
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Abstract

Objectives : The process through which mesenchymal cells condense and differentiate into chondrocytes to form new bone is known as endochondral bone formation. Chondrogenic differentiation and hypertrophy are essential steps in bone formation and are influenced by various factors. The stem bark and root bark of Eleutherococcus sessiliflorus (ES) have been widely used to treat growth retardation and arthritis in traditional Korean Medicine. In this study, we aimed to investigate the possible role of the stem bark of ES in the stimulation of chondrogenic differentiation in clonal murine chondrogenic ATDC5 cells. Methods : In ATDC5 cells treated with ES extract, cell viability and extracellular matrix production were determined using CCK-8 assay and Alcian blue staining, respectively, and alkaline phosphatase activity was measured. We also examined mRNA and protein expression levels of genes related to chondrogenic expression in ATDC5 cells using reverse transcription-polymerase chain reaction and western blot analyses. Results : ES extract increased the accumulation of Alcian blue-stained cartilage nodules and alkaline phosphatase activity in ATDC5 cells. It increased the mRNA expressions of chondrogenic markers including bone sialoprotein (BSP), cartilage collagens, Runt-related transcription factor-2 (RUNX-2), osteocalcin (OCN), β-catenin, and bone morphogenetic protein-2 (BMP-2), as well as the protein expressions of β-catenin, RUNX-2, BMP-2, and alkaline phosphatase (ALP). Conclusion : Taken together, these results suggest that ES extract exhibits a chondromodulating activity and therefore may be a possible agent for the treatment of bone growth disorders.

Keywords

Acknowledgement

이 논문은 2021 전북대학교의 학술연구지원을 받아 수행된 연구로 이에 감사드립니다. 또한 본 연구는 보건복지부의 재원으로 한국보건산업진흥원의 한의약선도기술개발사업 지원에 의하여 이루어진 것입니다(HI14C0976).

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