• Development and Reproduction
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• v.17 no.4
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• pp.353-361
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• 2013

The objective of this study was to characterize the reproductive cycle of the chameleon goby, T. trigonocephalus. Gonadal development was investigated using a histological method. Specimens were collected monthly, from April 2009 to March 2010. The gonadosomatic index (GSI) of females began to increase in April, reaching the maximum in May, and declined sharply in August. In males, the GSI began to increase in April and reaching the maximum in July. The annual reproductive cycle of T. trigonocephalus can be divided into four successive stages in females: the growing (November-March), maturing (April-May), ripe and spawning (June-July), and recovery (August-October) stages. Males passed through growing (November-March), maturing (April-June), ripe and spermiation (July-August), and recovery (September-October) stages. These results indicate the spawning season is from June to July. The relationship between fecundity (Fc) and body length (BL) was $Fc=86.1511BL^{2.6506}$. Fecundity was ranged from 3,448-9,654 eggs in a BL of 4.8-7.2 cm and it was increased as BL increased.

• Korean Journal of Agricultural Science
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• v.5 no.2
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• pp.77-79
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• 1978

The Present study was examined the response of mice of 5 strains to superovulation treatment. Female mice of CF#1, C57BL/6, ICR, dd and CHN strains, aged 60 days were used. Superovulation was induced by the subcutaneous injection of 5 IU PMS and followed 48 hours later by the subcutaneous injection of 5 IU HeG. All females were killed 24 hours after RCG and then ova in the oviduct were counted under a microscope. The average number of ova ovulated in CF#1, C57BL/6, ICR, dd and CHN were 21.3, 17.9, 20.7, 21.0 and 20.5, respectively.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.1
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• pp.74-83
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• 1995

To estimate the parameters of a mathematical model of fishes' swimming behavior, the behavior in a experimental water tank was observed and analyzed using the video monitoring system. The tank was equipped with vertical circulation system, and measured $3,500L\;{\times}\;1,500B\;{\times}\;1,000H\;mm$ at flow channel and $1,200L\;{\times}\;900B\;{\times}\;500H\;mm$ at observational part. Rainbow trout, salmo gairdnerii were used as experimental fishes. Their swimming behavior in the tank was observed by the monitoring system, and the positions of every individual were checked at 0.5 second intervals by the image processing of recorded pictures for 5 minutes. The mean swimming speed calculated from the time series data of positions of every individual ranged from 2.5BL cm/sec to 2.9BL cm/sec at the stagnated flow. The mean swimming speed of 10 individuals in a school increased according to the flow speed. The mean swimming depth ranged from 17 cm to 38 cm even though it changed irregularly at the stagnated flow and gradually became stable according to the increase of flow speed. In the present study, the mean distance of individuals from wall of the tank varied from 17.6cm to 21.4cm. The mean distance between the nearest individual varied from 0.4BL cm to 0.7BL cm when 10 individuals in a school were observed. The mean dimension of fish schools became enlarged in all directions according to increase in the number of individuals, and as flow speed increased the horizontal dimension of fish schools expanded while their vertical dimension decreased.

• Korean Journal of Acupuncture
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• v.35 no.2
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• pp.105-115
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• 2018

Objectives : This research aimed to verify the validities of contraindicated acupoints in acupuncture treatment in Zhenjiudacheng. Methods : We investigated contraindicated acupoints when performing needling in Zhenjiudacheng, then verified them in today's medical knowledge and clinical studies. Results : In Zhenjiudacheng, 39 acupoints - LU2, LI4, LI13, LI14, LI16, ST1, ST9, ST12, ST17, ST25, ST30, ST42, SP6, SP11, SI18, BL8, BL9, BL56, KI11, TE7, TE8, TE19, TE20, GB3, GB18, GB21, GV10, GV11, GV17, GV22, GV24, CV1, CV4, CV5, CV8, CV9, CV15, CV17 and EX-HN11 - were described to need a careful approach in acupuncture treatment and 21 acupoints among them had contraindications. Most of the contraindications can be explained based on anatomical knowledge and results of clinical studies, and acupuncture treatment at the 39 contraindicated acupoints can induce injury of tissues or organs near the acupoints. Conclusions : The contraindicated acupoints in acupuncture treatment in Zhenjiudacheng seem to have been classified based on the adverse events experienced by doctors at that time. Therefore careful acupuncture treatment at these acupoints is needed to avoid injury to tissue or organs.

• The Korea Journal of Herbology
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• v.32 no.5
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• pp.47-55
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• 2017

Objectives : Obesity is caused by the excess accumulation of fat in the body due to energy imbalance, and it causes various diseases. The aim of this study was to investigate an anti-obesity efficacy and an antioxidant activity of water from herbal mixture extract (SM17). Methods : The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. To evaluated anti-obesity effect of SM17, we used a high fat diet fed mouse model. The SM17 (150 mg/kg body weight/day, p.o.) was treated every day for 6 weeks to C57BL/6 mice. Body weight and food intake were measured every day. The changes of reactive oxygen species (ROS), alanine aminotransferanse (ALT), aspartate aminotransferase (AST), triglycerids (TG) and total cholesterol (TC) in serum were analyzed after experiment. Also, expression of lipid metabolism related proteins were investigated by western blot analysis. Results : It was effective in antioxidant measurements, SM17 administration inhibited the biomarkers of lipid metaboism in serum and tissues. The administration of SM17 showed a significant reduction of body and tissue weight. Morever, it decreased ROS, ALT, AST, TG and TC in serum, compared with those of the obese mice. Adipogenesis-related protein expressions increased in obese mice compared to normal mice. However, SM17 group exhibited the down-expression of these proteins. Conclusion : A SM17 aqueous extract has a great effect on the stimulation (AMPK) activation, and may have a benefit to reduce a fatty acid metabolism through inhibition of lipid accumulation.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.79-79
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• 2002

본 연구에서는 in vitro에서 성숙된 난자의 핵성숙(Polar Body extrusion)에 소요되는 시간과 배반포 단계로의 발달능력 사이의 관계를 비교하여 조기에 발달능력을 가진 embryo를 선발할 수 있는 IVP 체계를 개발하고자 하였으며 in vitro maturation(IVM)에 따른 first polar body(PB) 형성, IVM과 IVF 시간이 oocyte의 발달에 미치는 영향과 생산된 배반포의 세포수를 평가하였다. IVM은 TCM199 배양액을 사용하였고 in vitro fertilization(IVF)은 Fer -TALP용액을 사용하였으며 in vitro culture(IVC)는 CRlaa 배양액을 사용하여 2일까지는 0.3% BSA를 3일 부터는 10%FBS와 bovine oviduct epithelial cell을 첨가하여 배양하였다. IVM 시간에 따른 PB의 출현율은 0hr(0%), 6hr(0%), 12hr(0%), 14hr(8.7%), 16hr(40.5%), 18hr(48.0%), 20hr(65%), 22(68%) 그리고 24hr(74.5%)을 보였으며 IVM 시간에 따른 cleavage 및 8cell 발달율 사이에는 유의적인 차이가 없었으나 배반포(BL) 및 8cell에서 배반포로 발달률은 18시간(BL 31$\pm$6, BL/8cell 82 $\pm$5%)에서 가장 높게 나타났으며 24시간(BL 17$\pm$2, BL/8cell 60$\pm$8%)과 유의적인 차이를 보였다(P<0.05). IVC 7일째 배반포의 총세포수와 trophoblast(TE) 세포수는 IVM 18시간(mean$\pm$S.E.; total: 131.1$\pm$34.0, TE: 97.6$\pm$29.6)에서 24시간(total: 112.2$\pm$17.5, TE: 80.1$\pm$15.6)보다 유의하게 많은 것으로 나왔으나(P<0.05) 7일째의 inner cell mass(ICM) 숫자(18hr 33.5$\pm$12.8 vs 24hr 32.1$\pm$12.0)와 8일째 ICM, TE 그리고 총 세포수에는 유의성 있는 차이가 없었다. IVM 18시간에서 PB 형성과 8cell 발달률 사이에 높은 상관성을 보였고 배반포 및 8cell에서 배반포 단계로 높은 발달률을 보였으며 생산된 배반포의 TE 숫자와 총 세포수가 유의하게 많은 것으로 나타났다. 따라서 IVM 18시간 실시하였을 경우 보다 많은 세포수를 가진 배반포 발달 가능성이 높은 embryo를 조기에 선발 가능할 것으로 사료된다.

• Korean Journal of Acupuncture
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• v.27 no.2
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• pp.57-70
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• 2010

Objectives : To investigate the meaning and the importance of needling or moxibustion- prohibited acupoints in Chimgugapeulgyeong(鍼灸甲乙經). Methods : We found needling or moxibustion-prohibited acupoints in Chimgugapeulgyeong, then investigated the causes of the prohibitions from the various literatures and the anatomical structures near the acupoints. Results : In Chimgugapeulgyeong, the needling and moxibustion-prohibited points were ST9, ST17, ST32, CV5, and CV15. The needling-prohibited points were LU2, LI13, ST12, BL56, KI2, KI7, TE8, TE19, GB3, CV8, GV24, and jwagak(左角). The moxibustion-prohibited points were LU3, LU8, ST1, ST7, ST8, ST30, ST33, BL5, BL6, BL15, BL30, TE18, TE21, TE23, GB22, GB33, GB42, GV6, GV15, GV16, GV17 and GV25. The major cause of developing prohibited needling or moxibustion was due to the possibility to damage vessels or organs near them; other causes were side effects brought by applying wrong stimulating method or inducing women sterilized. Conclusions : The prohibition of needling or moxibustion on the points found in Chimgugapeulgyeong had acceptable causes. Therefore the techniques of needling or moxibustion on the acupoint should be performed with care.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.149-153
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• 2003

This study was conducted to survey the utilization possibility of composting system of small scale static pile with animal manure produced from cattle shed and the effect of addition of microorganism on the maturity of compost. Microorganisms added in composting substrate were bacteria+lactobacillus(BL)+photo.(BLP). The composting practiced was a windrow system without aeration equipment and turning was practiced periodically. The water content of substrate mixed with cow manure, rice husk, and sawdust was about 60%. The temperature during the composting process was increased at over $60^{\circ}C$ within 3 days after composting starting. Increase of temperature at the early stage of composting was fasten in BLP and BL than Control. Because the pH of the raw material was high, the changes of pH during composting was little and stabilized in weak alkaline condition. EC value was high for accumulation of manure and urine excreted continuously by animal and the changes of those during composting occurred in 5~10% increase. Reduction rates of C/N ratio were the largest as the 22.7% in BLP and 19.2and 17.5% in BL and Control respectively. In the evaluation of phytotoxicity, there was stabilized within the short time in BLP and not the difference between BL and Control. Treatment of animal manure produced from small scale cattle shed was possible by using the small scale static pile composting system with reasonable water content and turning and the addition of microorganism in composting substrate was effected on the temperature increase at the early stage of composting and reduction of plant toxicity compounds but little on the maturity of compost.

• Journal of Haehwa Medicine
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• v.15 no.1
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• pp.33-40
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• 2006

Objectives : This study is aimed to investigate acupuncture treatments for blurred vision, tinnitus, difficulty in urination and bi-syndrome caused by aging process through oriental medical documents. methods : We researched 21 oriental medical classics for the acupuncture treatments of blurred vision, tinnitus, difficulty in urination and bi-syndrome caused by aging or deficiency. Results & conclusions : 1. Disharmony between yin and yang, disharmony between organs, disharmony of essence, qi, spirit, blood and body fluid are the main features of aging. 2. ST36, BL18, BL10, CV24, ST1, BL23 have been most frequently used for acupuncture to treat blurred vision caused by aging. For moxibustion, ST36 has been most frequently used. 3. GB2, TE21, TE17, SI19, TE3 are the mainly used points for acupuncture to treat tinnitus and deafness caused by aging. For moxibustion, LI1 and BL15 have been most frequently used. 4. CV3, CV7, CV4, BL67, LR8, LR4 have been most frequently used to treat difficulty in urination caused by aging. 5. LI11, GB34, GB30, LI4, ST36, TE10, TE5, Sama (extra point) have been most frequently used to treat numbness, paralysis and bi syndrome caused by aging.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.626-632
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• 2005

A new bacterium BL-2 excreting a novel cationic polyglucosamine biopolymer was isolated from the spoiled leaves of Chinese cabbage and identified as Enterobacter sp. BL-2. The isolated Enterobacter sp. BL-2 was cultivated in pH-stat fed-batch culture using acetic acid as the feeding stock at pH 8.0, resulting in 17.11 g/l of cells and 1.53 g/l of an extracellular biopolymer after 72 h. The excreted biopolymer was purified by a three-step procedure, involving ethanol precipitation and deproteinizations, to a nearly homogeneous state, and its molecular weight was found to be 106 kDa. It was composed of glucosamine, rhamnose, and galactose at a molar ratio of 86.4:1.6:1.0, respectively, indicating a rarely found novel high-glucosamine-containing biopolymer. The FT-IR and $^{13}C-NMR$ spectra of the novel cationic polyglucosamine biopolymer PGB-l revealed a close identity with chitosan from crab shell. It can effectively flocculate various suspended solids, including kaolin clay, $Ca(OH)_2,\;Al_{2}O_3$, active carbon, microbial cells, and acidic dyes.