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Effect of Harvest Time and Infective Juvenile Size of the Entomopathogenic Nematode, Steinernema arenarium, on Pathogenicity, Development, and Propagation (곤충병원성선충, Steinernema arenarium의 수확시기와 유충 체장이 병원성과 발육 및 증식에 미치는 영향)

  • Han, Gun-Yeong;Lee, Dong-Woon;Choo, Young-Moo;Choo, Ho-Yul
    • Korean journal of applied entomology
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    • v.51 no.1
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    • pp.9-18
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    • 2012
  • The size of infective $Steinernema$ $arenarium$ juveniles is variable and ranges from 724 to 1408 ${\mu}m$. Effects of harvest time and infective juvenile size on pathogenicity, development, and reproduction were examined in the last instar of the great wax moth, $Galleria$ $mellonella$. Harvest time of infective juveniles (IJs) of $S.$ $arenarium$ affected pathogenicity. IJs harvested at the 10th day from trapping were more pathogenic than those harvested the 3rd day from trapping. Mortality of $G$. mellonella also depending on harvest time, $i.e$, 100% died within 48h when IJs were harvested at the 10th day, without relation to size. However, mortality was 40% in the small size group (SSG) compared with 18% in the large size group (LSG) within 48h when IJs were harvested at the 3rd day. Establishment of $S.$ $arenarium$ within the host was different depending on IJ size. The number of established IJs was 1.8 in the SSG, 3.3 in the LSG, and 3.2 in the mixed size group (MSG) when IJs were harvested at the 3rd day, and 5.3 in the SSG, 7.4 in the LSG, and 7.6 in the MSG when IJs were harvested at the 10th day. The length of the female adult was 7,070.5 ${\mu}m$ in the SSG and 7,893.9 ${\mu}m$ in the LSG and that of the male was 1,460.5 ${\mu}m$ in the SSG and 1,688.2 ${\mu}m$ in the LSG when IJs were harvested at the 3rd day. The length of the female adult was 7,573.6 ${\mu}m$ in the SSG and 8,305.4 ${\mu}m$ in the LSG and that of the male adult was 1,733.4 ${\mu}m$ in the SSG and 1,794.4 ${\mu}m$ in the LSG when IJs were harvested at the 10th day. Harvest time and size of IJs did not influence numbers of progeny or size of IJS.

Antibacterial Activity of Sodium Phytate and Sodium Phosphates Against Escherichia coli O157:H7 in Meats (식육에서 피틴산염과 인산염의 Escherichia coli O157:H7균에 대한 항균효과)

  • Hue, Jin-Joo;Li, Lan;Lee, Yea-Eun;Lee, Ki-Nam;Nam, Sang-Yoon;Yun, Young-Won;Jeong, Jae-Hwang;Lee, Sang-Hwa;Yoo, Han-Sang;Lee, Beom-Jun
    • Journal of Food Hygiene and Safety
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    • v.22 no.1
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    • pp.37-44
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    • 2007
  • The approval of use of certain food-grade phosphates as food additives in a wide variety of meat products greatly stimulated research on the applications of phosphates in foods. Although phosphates have never been classified as antimicrobial agents, a number of investigators have reported that phosphates have antimicrobial activities. Phytic acid is a natural plant inositol hexaphosphate constituting 1-5% of most cereals, nuts, legumes, oil seeds, pollen, and spores. In this study, we investigated antibacterial activities of sodium phytate(SPT), sodium pyrophosphate (SPP), sodium tripolyphosphate (STPP) on Escherichia coli O157:H7 on tryptic soy broth and in beef, pork and chicken. In tryptic soy broth, SPT, SPP and STPP at the concentrations of 0.05, 0.1, and 0.5% effectively inhibited the growth of Escherichia coli O157:H7 in a concentration-dependent manner. The bactericidal activity of SPT was the stronger than that of SPP or STPP at the same concentrations. In addition, the antibacterial effects of SPT, SPP and STPP at the concentrations of 0.05, 0.1, 0.3, and 0.5% on Escherichia coli O157:H7 were also investigated in raw or cooked meats including beef, pork and chicken. SPT, SPP and STPP significantly inhibited the bacterial growth in a dose-dependant manner (p<0.05). The bactericidal effect of SPT was stronger than that of SPP or STPP. The addition of SPT, SPP and STPP in meats increased meat pHs. SPP and STPP also increased the levels of soluble orthophosphate in meats but STP did not. These results indicate that SPT is very effective for inhibition of bacterial growth and that can be used as a muscle food additive for increasing functions of meats.

The Effect of Hyeonggaeyeongyotang Extract on the Fertility, Early Embryonic Development in Wistar rats by Oral Gavage Administration (형개련교탕(荊芥連翹湯) 추출물의 경구투여(經口投與)가 rat의 수태능(受胎能) 및 초기(初期) 배발생(胚發生)에 미치는 영향(影響))

  • Kim, Eun-Hee;Hwang, Sun-Yi;Kim, Sang-Chan;Jee, Seon-Young
    • Herbal Formula Science
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    • v.16 no.1
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    • pp.65-78
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    • 2008
  • Objectives : this study was to access the effect of Hyeonggaeyeongyotang water extracts, a polyherbal formula has been used as folk medicine, on the fertility and early embryonic development of male and female Wistar rats when administered by oral gavage. Methods : In male rats, Hyeonggaeyeongyotang extract were dosed 4 weeks before pairing and 2 weeks after mating including the mating periods up to termination after necropsy of the majority of the females. In female rats, they were dosed 2 weeks before pairing, and from Day 0 to Day 7 of gestation. This study was conducted in accordance with the recommendations of the KFDA Guideline [2005-60] for Detection of Toxicity to Reproduction for Medicinal Products. Results: 1. No Hyeonggaeyeongyotang extract treatment-related changes on the clinical signs and mortalities, the Food consumptions, the Body weights and gains were demonstrated in all dosed levels tested in this study except for 500ml/kg-dosing male group in which a significant(p<0.05) increase of body gains was detected during day 0-7 after dosing. 2. No Hyeonggaeyeongyotang extract treatment-related changes on the pre-coital intervals, the estrus cycles, the mating index, conception rate and fertility index were demonstrated in all dosed levels tested in this study. 3. No Hyeonggaeyeongyotang extract treatment-related gross findings on reproductive organs, the weights of reproductive organs, histopathological findings on reproductive organs, the corpora lutea number, implantation site number, live fetus number, number of resorpted embryo and pre-and post-implatation loss were demonstrated in all dosed levels tested in this study. Conclusions : Base on the results, it is considered that the NOAEL (No-Observed-Adverse-Effect Level) for fertility and early embryonic development toxicity of Hyeonggaeyeongyotang extract was under 2000ml/kg/day in Wistar male and female rats because there no treatment-related changes on the fertility and early embryonic developmental index were demonstrated in all dosed levels tested.

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Feasibility Study for the Cleaning of Well Screens using High-voltage Pulsed Discharge (고전압 펄스 방전을 이용한 지하수 관정 스크린 공막힘 재생법 연구)

  • Chung, Kyoung-Jae;Lee, Seok-Geun;Dang, Jeong-Jeung;Choi, Gil-Hwan;Hwang, Y.S.;Kim, Chul-Young;Park, Young-Jun
    • The Journal of Engineering Geology
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    • v.23 no.1
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    • pp.29-36
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    • 2013
  • The application of appropriate rehabilitation methods can improve the efficiency of clogged wells and extend their life. In this paper, we study the feasibility of well cleaning using high-voltage pulsed discharge, in which electrical energy is used to produce impulsive pressure in water, in contrast to conventional methods that employ chemical or pneumatic energy sources. This technique utilizes the compressive shock wave generated by the expansive force of hot, dense plasma that is produced during a pulsed discharge in the gap between electrodes immersed in water. Compared with conventional techniques, this method is simple, and easy to handle and control. Using a capacitive pulsed power system with an electrical energy of 200 J, an impulsive pressure of 10.7 MPa is achieved at the position 6 cm away from the discharge gap. The amplitude of the impulsive pressure was easily controlled by adjusting the charging voltage of the capacitor and was almost linearly proportional to peak discharge current. The technique achieved good results in cleaning feasibility tests with mock-up specimens similar to clogged well screens.

Differences in the Joint Movements and Muscle Activities of Novice according to Cycle Pedal Type

  • Seo, Jeong-Woo;Kim, Dae-Hyeok;Yang, Seung-Tae;Kang, Dong-Won;Choi, Jin-Seung;Kim, Jin-Hyun;Tack, Gye-Rae
    • Korean Journal of Applied Biomechanics
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    • v.26 no.2
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    • pp.237-242
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    • 2016
  • Objective: The purpose of this study was to compare the joint movements and muscle activities of novices according to pedal type (flat, clip, and cleat pedal). Method: Nine novice male subjects (age: $24.4{\pm}1.9years$, height: $1.77{\pm}0.05m$, weight: $72.4{\pm}7.6kg$, shoe size: $267.20{\pm}7.50mm$) participated in 3-minute, 60-rpm cycle pedaling tests with the same load and cadence. Each of the subject's saddle height was determined by the $155^{\circ}$ knee flexion angle when the pedal crank was at the 6 o'clock position ($25^{\circ}$ knee angle method). The muscle activities of the vastus lateralis, tibialis anterior, biceps femoris, and gastrocnemius medialis were compared by using electromyography during 4 pedaling phases (phase 1: $330{\sim}30^{\circ}$, phase 2: $30{\sim}150^{\circ}$, phase 3: $150{\sim}210^{\circ}$, and phase 4: $210{\sim}330^{\circ}$). Results: The knee joint movement (range of motion) and maximum dorsiflexion angle of the ankle joint with the flat pedal were larger than those of the clip and cleat pedals. The maximum plantarflexion timing with the flat and clip pedals was faster than that of the flat pedal. Electromyography revealed that the vastus lateralis muscle activity with the flat pedal was greater than that with the clip and cleat pedals. Conclusion: With the clip and cleat pedals, the joint movements were limited but the muscle activities were more effective than that with the flat pedal. The novice cannot benefit from the clip and cleat pedals regardless of their pull-up pedaling advantage. Therefore, the novice should perform the skilled pulling-up pedaling exercise in order to benefit from the clip and cleat pedals in terms of pedaling performance.

Feeding Habit Analysis of the Korean Water Deer (고라니(Hydropotes inermis argyropus)의 식이습성 분석)

  • Park, Ji-Eun;Kim, Baek-Jun;Oh, Dae-Hyun;Lee, Hang;Lee, Sang-Don
    • Korean Journal of Environment and Ecology
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    • v.25 no.6
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    • pp.836-845
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    • 2011
  • This study aims feeding habit of the Korean water deer(Hydropotes inermis argyropus) using a visual analysis of rumen contents of 44 individuals. The samples were collected from Cheorwon(Gangwon province) and the eastern part of Jeonnam province. Based on the analysis, a total of 15 orders, 18 families and 13 species were identified. Among them, Solanceae(53.8%) and Graminease(30.3%) showed the highest proportions. Plants of 13 orders, 15 families and 10 species were found and forbs(70.21%) and Solanceae(54.9%) were most preferred in Cheorwon. Eight orders, 7 families and 4 species were found in the Eastern parts of Jeonnam province, and Gramineae(78.6%) and woody plants(14.70%) was most preferred. Among four seasons, Gramineae in spring(80.6%) and autumn(68.8%), Leguminosae(75.9%) in summer, and Solanceae(85.4%) in winter were the highest proportions.

[$Ca^{2+}$ Sensitization Mechanism in Stretch-induced Myogenic Tone

  • Kim, Jung-Sup;Ryu, Sung-Kyung;Ahn, Duck-Sun;Kang, Bok-Soon;Lee, Young-Ho
    • The Korean Journal of Physiology and Pharmacology
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    • v.6 no.1
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    • pp.33-39
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    • 2002
  • It has been suggested that $Ca^{2+}$ sensitization mechanisms might contribute to myogenic tone, however, specific mechanisms have not yet been fully identified. Therefore, we investigated the role of protein kinase C (PKC)- or RhoA-induced $Ca^{2+}$ sensitization in myogenic tone of the rabbit basilar vessel. Myogenic tone was developed by stretch of rabbit basilar artery. Fura-2 $Ca^{2+}$ signals, contractile responses, PKC immunoblots, translocation of PKC and RhoA, and phosphorylation of myosin light chains were measured. Stretch of the resting vessel evoked a myogenic contraction and an increase in the intracellular $Ca^{2+}$ concentration $([Ca^{2+}]_i)$ only in the presence of extracellular $Ca^{2+}$. Stretch evoked greater contraction than high $K^+$ at a given $[Ca^{2+}]_i.$ The stretch-induced increase in $[Ca^{2+}]_i$ and contractile force were inhibited by treatment of the tissue with nifedipine, a blocker of voltage-dependent $Ca^{2+}$ channel, but not with gadolinium, a blocker of stretch-activated cation channels. The PKC inhibitors, H-7 and calphostin C, and a RhoA-activated protein kinase (ROK) inhibitor, Y-27632, inhibited the stretch-induced myogenic tone without changing $[Ca^{2+}]_i.$ Immunoblotting using isoform-specific antibodies showed the presence of $PKC_{\alpha}$ and $PKC_{\varepsilon}$ in the rabbit basilar artery. $PKC_{\alpha},$ but not $PKC_{\varepsilon},$ and RhoA were translocated from the cytosol to the cell membrane by stretch. Phosphorylation of the myosin light chains was increased by stretch and the increased phosphorylation was blocked by treatment of the tissue with H-7 and Y-27632, respectively. Our results are consistent with important roles for PKC and RhoA in the generation of myogenic tone. Furthermore, enhanced phosphorylation of the myosin light chains by activation of $PKC_{\alpha}$ and/or RhoA may be key mechanisms for the $Ca^{2+}$ sensitization associated with myogenic tone in basilar vessels.

LONG-TERM ANALYSIS OF RECONSTRUCTED TEMPOROMANDIBULAR JOINT AND MANDIBLE USING FREE FIBULAR FLAP (비골 피판을 이용한 하악 및 하악과두 재건의 장기간 임상적 평가)

  • Ahn, Kang-Min;Chung, Hun-Jong;Ryom, Hak-Ryol;Kim, Hang-Jin;Kim, Yoon-Tae;Hwang, Soon-Jung;Myoung, Hoon;Kim, Myung-Jin;Kim, Soung-Min;Jahng, Jeong-Won;Lee, Jong-Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.31 no.5
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    • pp.409-416
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    • 2005
  • Purpose of study: The temporomandibular joint (TMJ) occupies a key functional role in mastication and contributes to normal deglutition, speech as well as cosmesis. When a large amount of mandible including the condyle head is resected, it is very difficult to reconstruct it as a functional unit. In this retrospective study, we present the functional, radiographic and cosmetic results of reconstructed temporomandibular joint using free fibular flap. Patients and Methods: Total 12 patients (M:F = 6:6) who underwent condylar reconstruction with the fibular flap were interviewed and examined by radiographs and Bio-PAK$^{(R)}$. Mean follow up periods was $47.7{\pm}20.0$ months and the average age was $38.7{\pm}15.3$ years. Remodeling of condyle and function of TMJ were evaluated and facial contour was judged subjectively. Results: All flaps were viable and no immediate postoperative complication had happened. One patient showed decreased mouth opening, so interpositional gap arthroplasty was performed. The resorption rates of reconstructed fibular were minimal and the condyle heads were changed into domeshaped neocondyle after 2 years. All patients had normal diet and no speech difficulty was reported. Nine patients were satisfied with their facial contour but three patients complained about the depression of cheek. Conclusion: The reconstruction of TMJ with free fibular flap was reliable methods and very effective means of restoring mandibular function. The functional and morphologic results were excellent and showed little complications.

Exploring the Student Presenters' and Student Visitors' Perceptions of the Science Festival (과학축전 활동 경험에 대한 부스 운영 및 참여 관람 학생의 인식)

  • Park, Eun-Ji;Lee, Sun-Kyung;Kim, Ki-Sang;Kim, Chan-Jong
    • Journal of the Korean earth science society
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    • v.31 no.7
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    • pp.772-784
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    • 2010
  • 'Science festival' is an annual public event featuring a variety of science- and technology-related workshops and live demonstrations of experiments by student presenters. This study was to explore the student presenters' and student visitors' perceptions of the science festival. The subjects were 323 student presenters who managed laboratory booths and 495 student visitors who attended the festival for six days. We developed a questionnaire based on the modified items of ones both from Ahn & Park (2009) and Lee et al. (2010). Data included students' participation backgrounds, differences of their perceptions about the educational effects according to participation types and school levels, and student presenters' perceptions of scientific inquiry that they had through the preparation of the festival. Findings suggested that student presenters perceived their experience of preparing for the festival as meaningful scientific inquiry process such as asking and solving problems. Based on the results, discussion and implications for communitybased programs as an informal science education were presented.

The Effect of University Hospital Budgeting System Characteristics on Budgetary Slack (대학병원 예산시스템의 특성이 예산슬랙에 미치는 영향)

  • Jung, Tae-young;Lee, Seo-joon;Han, Jae-hoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.2
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    • pp.405-412
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    • 2018
  • The budget is an essential tool for the systematic management of the organizational performance and for diagnosing the status of an organization. Effective budget execution is important to hospital management due to the nonprofit nature of hospitals. On the other hand, there are few studies regarding the budgetary slack of hospitals in Korea. The purpose of this study was to investigate the effects of the university hospital budgeting system characteristics on the budgetary slack. For this purpose, the data from 118 staff of a university hospital located in Seoul were collected by self-administered surveys. Frequency analysis, correlation analysis, and multiple regression analysis were carried out using Stata Ver. 14. The main results of this study were as follows. First, information asymmetry has a positive influence on the budgetary slack. Second, budget feedback and budget motivation have a negative effect on the budgetary slack. These findings suggest that the sharing of budget-related information among hospital staff is important for efficient hospital management. In addition, it is necessary to establish ways to enhance the feedback and motivation in budgetary activities for managing the budgetary slack systematically. The major strength of this study is that it draws attention to research concerning the budgetary slack of hospitals in the absence of research on the budgetary slack. The significance of this study was to provide an empirical basis for improving the efficiency of hospital management.