• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.485-488
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• 2000

Degradation of aromatic compound by Pseudomonas mendocina BCNU 154 has been investigated. The microorganism utilizes xylene, toluene, nitrobenzene, ethylbenzene and cumene. This strain is tolerant to some heavy metals, such as Mn, Cu, Si, and Mo, and resistant to some antibiotics, such as vancomycin, chloramphenicol and ampicillin. The metabolic pathway of toluene in Pseudomonas mendocina BCNU 154 is also elucidated.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.474-477
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• 2000

An organic solvent-tolerant Pseudomonas savastanoi strain BCNU 106 was isolated.. This bacterium was gram negative rod, and showed oxidase positive, catalase positive, and decaboxylase positive reactions. The Pseudomonas strain oxidized cholesterol on the medium supplemented with an organic solvent. The strain BCNU 106 is able to grow in presence of organic solvents of which log $P_{ow}$ is between 1.5 to 7.0. The strain was well grown in a medium supplemented with a 10% volume of a organic solvent (toluene, p-xylene, cyclohexane, mesitlylene) containing $cholesterol(20mg/m{\ell)$. Major conversion compound were 7-dehydrocholesteryl dimethylphosphate, cholesta-4,7-dien-3-one, and cholesta-3,5-dieone.

• Journal of Life Science
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• v.14 no.1
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• pp.188-192
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• 2004

Toluene tolerance and therrnotolerance in Crampositive organic solvent resistant bacterium Micrococcus sp. BCNU 121 has been studied. Exposure to a sub- lethal temperature or a sub-lethal concentration of toluene conferred protection to subsequent challenges with a killing temperature or a lethal concentration of toluene, respectively. Pretreatment of Micrococcus sp. BCNU 121 with sub-lethal concentrations of toluene induced adaptative protection against heat shock. Moreover, temperature-adaptative cells also showed cross-resistance to lethal doses of toluene. These data suggested a cross-regulation between toluene tolerance and heat shock response.

• Proceedings of the Korean Society of Life Science Conference
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• 2001.02a
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• pp.64-64
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• 2001

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.374-377
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• 2000

A strain of Pseudomonas sp. BCNU 154 was isolated from contaminated soil with orangic solvents as the sole source of carbon and energy. It utilized an exceptionally wide aromatic substrates. The strain BCNU 154 was able to utilize toluene, p-xylene, ethylbenzene, cumene, as the only carbon and energy source. When toluene or p-xylene was used as the sole carbon and energy source, the compound was rapidly degraded with significant increase in biomass concentruction. The biodegradation of this compound was observed when ethylbenzene or cumene was supplied on the carbon source and energy source, which may be a candidate extremophilic bacterium for the bioremediation technology.

• Biomolecules & Therapeutics
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• v.3 no.2
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• pp.122-131
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• 1995

Five human cancer cell lines (HeLa S3, Hep 3B, KATO III, Hs 683, HeLa MR) and one human normal cell line (WI-38) were examined cell viability, northern blot analysis, western blot analysis, and in situ hybridization for the expression $O_{6}$ -methylguanine-DNAmethyltransferase (MGMT), which can repair $O_{6}$ -methylguanine produced in DNA by alkylating agents. In cell viability test, the lethal sensitivities of each strain against anti-tumor drug N,N-bis(2-chloroethyl)- N-nitrosourea (BCNU) were counted, and both BCNU treated and untreated cell extracts were examined for their MGMT inducibility by RNA dot blot analysis. Cell lines did not show MGMT induction by BCNU pretreatment. Tlle MGMT activity was assayed by measuring the $^3$H radioactivity transferred from the substrate DNA containing [methyl-$^3$H)-O$_{6}$ -methylguanine to acceptor molecules in the cell extracts. Extracts from the majority of tumor strains and normal cells contained substantial MGMT activity of varying degree, while the known Mer$^{[-10]}$ cell (lacked or severely depleted in MGMT activity) Hela MR, and Hs 683 (proved to be Mer$^{[-10]}$ ) were much more sensitive to BCNU than the rest of tumor strains, as measured by cell viability test. Overall results above, KATO III showed the highest expression level of MGMT among the strains examined. Furthermore, with all the tumor and normal strains tested, a good correlation was observed between MGMT expression and cellular resistance to BCNU. The varying levels of expression of MGMT in human cancer cells found in this study should provide a molecular basis for MGMT expression among tumor strains from different tissue origin, the information of antitumor agents selection for chemotherapy of cancers.

• KSBB Journal
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• v.26 no.2
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• pp.100-106
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• 2011

Strain BCNU 5011 was isolated from forest soil samples collected in the Taebaek mountain in the Gangwon province, Korea. The biochemical, physiological and 16S rRNA sequence analysis strongly indicated that this isolate was most closely related to Paenibacillus polymyxa. A maximum production level of antimicrobial substances of Paenibacillus sp. BCNU 5011 was achieved under aerobic incubation at $30^{\circ}C$ for 3 days in SST broth.Paenibacillus sp. BCNU 5011 showed a broad spectrum of activity against Gram positive and Gram negative bacteria, including methicllinresistant Staphylococcus aureus (MRSA). Paenibacillus sp. BCNU 5011 was also shown to inhibit the growth of different potential human pathogenic bacteria and fungi in vitro. Peptide extract showed better antimicrobial activity than solvent extracts. But active antimicrobial compounds might be included in both peptide extract and solvent extracts. Further separation, purification and identification of active principles leads project to develop antimicrobial agents and anti-MRSA agents.

• Journal of Life Science
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• v.27 no.8
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• pp.945-950
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• 2017

To some extent, the growth of solvent-tolerant Pseudomonas sp. BCNU 106 is limited by toxic solvents. Therefore, various strategies to overcome this limitation need to be investigated. One such strategy is to use exogenous trehalose. The highest intracellular trehalose content of 181.88 mM was measured at 12 hr. The extracellular trehalose content decreased rapidly within 12 to 16 hr in the presence of cyclohexane. Moreover, the number of Pseudomonas sp. BCNU 106 cells grown in Luria-Bertani (LB) broth supplemented with 0.1 M trehalose in the presence of 1%(v/v) cyclohexane, hexane, propylbenzene, and m-xylene increased 89.94-, 89.72-, 91.25-, and 118.9-fold, respectively, in comparison to the control level. High survival rates of 80% and 90% were observed in the presence of cyclohexane and hexane by the addition of 0.05 M trehalose for up to 4 hr, respectively. Exogenously-added trehalose was transported into the cells, and it conferred protection against cyclohexane, hexane, propylbenzene, and m-xylene. Adding exogenous trehalose to the growth medium improved the tolerance of Pseudomonas sp. BCNU 106; thus, it is a potential biocatalyst for biotransformation and biodegradation.

• Microbiology and Biotechnology Letters
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• v.45 no.2
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• pp.162-167
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• 2017

For the production of bioethanol by the synchronous saccharification and fermentation (SSF) process, bio-capsule formation was attempted. Many saccharifying fungal strains and fermentative yeast strains were first screened. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201, and P. chrysogenum KACC 44363 were found to be excellent producers of saccharifying enzymes such as ${\alpha}$-amylase and glucoamylase. Saccharomyces cerevisiae IFO-M-07 showed the highest ethanol productivity among the tested strains. Secondly, we determined the optimal conditions for pellet formation, and those for bio-capsule formation. All the tested fungal strains formed pellets, and the optimal conditions for bio-capsule formation were $28^{\circ}C$ and 120 rpm. Lastly, SSF process was performed using a bio-capsule. An ethanol yield of 3.9% was achieved by using the Aspergillus sp. BCNU 6200 bio-capsule (Aspergillus sp. BCNU 6200 + S. cerevisiae IFO-M-07) at $30^{\circ}C$ with shaking at 120 rpm during the 10 days of incubation. The results provide useful information on the application of a bio-capsule in bioethanol production under the SSF process.

• Microbiology and Biotechnology Letters
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• v.45 no.4
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• pp.299-304
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• 2017

Biogenic amines such as histamine, tyramine, cadaverine, and putrescine may have detrimental effects on consumers' health; therefore, they must be considered hazardous substances in foods. In recent years, the application of microorganisms that can degrade biogenic amines has become an emerging method for reducing the amount of these amines in foods. Primarily, Bacillus sp. BCNU 9171 was isolated from Doenjang, a Korean traditional fermented soybean paste. The inhibitory effects of the cell-free supernatant (CFS) of Bacillus sp. BCNU 9171 on biogenic amine production by 4 amine-positive food pathogenic bacteria were investigated using high performance liquid chromatography (HPLC). Our results showed that 4 different CFS concentrations-10% [1 ml CFS + 9 ml histidine decarboxylase broth (HDB)], 25% (2.5 ml CFS + 7.5 ml HDB), 50% (5 ml CFS + 5 ml HDB), and 75% (7.5 ml CFS + 2.5 ml HDB)-reduced the biogenic amine production up to 87% compared with that of the control without CFS. These results suggested that it is advisable to use Bacillus sp. strain BCNU 9171 as a starter organism for the manufacture of fermented foods and to ensure food safety since it prevents the accumulation of high amounts of biogenic amines in fermented foods by amine-positive bacteria.