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http://dx.doi.org/10.4014/mbl.1702.02004

Bio-capsule Formation for Synchronous Saccharification and Fermentation Process  

Shin, Gyeong Yeon (Department of Biology and Chemistry, Changwon National University)
Choi, Hye Jung (Department of Biology and Chemistry, Changwon National University)
Kang, Yang-Rae (Il San Institute, Chilseo Ethanol Factory, Il San Trading Co., Ltd.)
Nam, Ki-Du (Il San Institute, Chilseo Ethanol Factory, Il San Trading Co., Ltd.)
Song, Ju Yeong (Department of Civil, Environmental and Chemical Engineering, Changwon National University)
Joo, Woo Hong (Department of Biology and Chemistry, Changwon National University)
Publication Information
Microbiology and Biotechnology Letters / v.45, no.2, 2017 , pp. 162-167 More about this Journal
Abstract
For the production of bioethanol by the synchronous saccharification and fermentation (SSF) process, bio-capsule formation was attempted. Many saccharifying fungal strains and fermentative yeast strains were first screened. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201, and P. chrysogenum KACC 44363 were found to be excellent producers of saccharifying enzymes such as ${\alpha}$-amylase and glucoamylase. Saccharomyces cerevisiae IFO-M-07 showed the highest ethanol productivity among the tested strains. Secondly, we determined the optimal conditions for pellet formation, and those for bio-capsule formation. All the tested fungal strains formed pellets, and the optimal conditions for bio-capsule formation were $28^{\circ}C$ and 120 rpm. Lastly, SSF process was performed using a bio-capsule. An ethanol yield of 3.9% was achieved by using the Aspergillus sp. BCNU 6200 bio-capsule (Aspergillus sp. BCNU 6200 + S. cerevisiae IFO-M-07) at $30^{\circ}C$ with shaking at 120 rpm during the 10 days of incubation. The results provide useful information on the application of a bio-capsule in bioethanol production under the SSF process.
Keywords
Bio-capsule; ethanol production; optimal conditions; saccharifying enzyme;
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