• BMB Reports
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• v.39 no.6
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• pp.782-787
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• 2006

A new protein was cloned and identified as the sixth subunit of Choristoneura fumiferana origin recognition complex (CfORC6). The newly identified 43 kDa protein CfORC6 is much bigger than DmORC6 (25.7 kDa) and HsORC6 (28.1 kDa), though it's 23.85% identical to DmORC6 and 23.81% identical to HsORC6. Although the molecular weight of CfORC6 is close to ScORc6 (50 kDa), CfORC6 is only 14.03% identical to ScORC6. By alignment, it was found that the N-terminal of CfORC6 has about 30% identities with other ORC6s, but about 100aa of C-terminal of CfORC6 has no identity with other ORC6s. Like ScORC6, CfORC6 has many potential phosphorylation sites, (S/T)PXK. Like DmORC6, CfORC6 has leucine-rich region in the relevant site. Northern Blot showed that CfORC6 mRNA is about 2,000nt. Southern Blot confirmed that there is one copy of CfORC6 gene in spruce budworm genome. Western blot showed that infection of Cf124T cells with CfMNPV didn't affect the expression levels of CfORC6, at least up to 26 hr post infection.

• Journal of muscle and joint health
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• v.27 no.3
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• pp.247-254
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• 2020

Purpose: This was a descriptive observational study examining the reasons for, frequency of, and factors affecting work interruption among intensive care unit (ICU) nurses. Methods: Convenience sampling was used to recruit 46 regular nurses who had been working in an ICU at D tertiary hospital in B metropolitan city for at least six months. To measure the degree of work interruption, this study revised a tool used by Brixey et al. (2007) for analyzing the type of work interruption. Results: A total of 1,787 work interruptions occurred during 368 hours of observation, i.e., at an average of 4.85 times per hour. Communication-related factors caused work interruption most frequently, followed by environmental, work-related, and personal factors. As for the work-related characteristics of nurses, participants experienced work interruption more frequently while working on weekdays, when the medical staff were stationed, than on weekends. Conclusion: ICU nurses experienced work interruption frequently. With the health care system expected to become more complicated in the future, efforts should be made to reduce unnecessary work interruptions to improve the operation efficiency of ICUs.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.6
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• pp.787-794
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• 2005

The aim of this study was 1) to confirm the practical efficiency of a routine milk P4 monitoring system for postpartum reproductive management of a dairy herd, and 2) to evaluate the relationship between the blood metabolic profiles, milk quality and body weight of individual cows in the farm records, which may reflect the postpartum nutritional condition, and the time of postpartum resumption of ovarian activity of dairy cows. A total of 116 Holstein cows was used in the present study. First, during the period of Experiment 1, postpartum reproductive management based on weekly measured milk P4 concentration from individual cows was conducted. Compared with the reproductive records of the past two years without P4 monitoring, although the day from calving to first AI did not change, both the number of AI until pregnant (with P4; 1.9 times vs. without P4; 2.9 times) and the days open (with P4; 95.1 days vs. without P4; 135.8 days and 133.8 days) were significantly decreased. In Experiment 2, the measurement of blood constituents such as albumin, blood urea nitrogen, packed cell volume, ammonia, glucose, total cholesterol, non-esterified, AST and $\gamma$-GTP was performed on the blood samples taken once approximately 14 days postpartum, to monitor both health and nutritional conditions. The milk constituent parameters, such as milk protein (MP), milk fat (MF), SNF and lactose, collected from the monthly progeny test of individual cows, were used to monitor the postpartum nutritional status. Furthermore, the data obtained from the routine measurements of body weight were used to calculate the rate of peripartum body weight loss. The resumption day of the postpartum estrous cycle was assumed from the milk P4 profiles of individual cows. There was no clear relationship between each parameter from blood examination and those from resumption time. However, the cows had low values of MP, and SNF, which significantly affected the resumption of the postpartum estrous cycle. Similarly, a higher rate of body weight loss indicated a significant delay (more than 1 month) in the resumption of the postpartum estrous cycle, compared with the groups that had a medium or lower rate of body weight loss. The results of the present study demonstrated that the implementation of routine milk P4 monitoring-based postpartum reproductive management, together with milk quality parameters and routine BW data available in field conditions may be utilized as a practical approach for increasing the postpartum reproductive efficiency of a high yielding dairy herd.

• Journal of Science Education
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• v.41 no.2
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• pp.179-194
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• 2017

The purpose of this study is to construct two equivalent science relevance recognition test tool after confirming the reliability and validity of the CARS(Changes in Attitude of Relevance to Science) questionnaire to determine the applicability of the items to Korean students and to compare gender and school differences. For this study, 59 items of the CARS scientific relevance test were translated and assigned to 787 middle and high school students (analyed the answer of 300 middle school students and 431 high school students). In order to determine the fit of the CARS question to Korean students and to overcome the limitation of the number of questions, we used the item-linking method of the Rasch model. By analyzing the results of the research, we constructed two equivalent scientific relevance recognition questionnaires of CARS-A and CARS-B with 25 items. The Pearson correlation coefficient of the Rasch scores of the two equivalent test was 0.78. The two types of scientific relevance recognition test tools generated through this study can be used to confirm students' attitude of scientific relevance to daily life, or to confirm the change after a certain class or grade. Through this study, we will discuss the implications of students' perceptions of science associations in science education, and the development and application of tools.

• Journal of agricultural medicine and community health
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• v.45 no.2
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• pp.89-99
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• 2020

Objective: This study aimed to report the relationship between walking activity and perceived physical activity barriers after classifying the age group(≤64, 65-74, ≥75). Methods: The subjects were conducted on 1500 residents of two areas, and the walking activity was defined as five or more times a week, 10 minutes or more at a time, and 30 minutes or more per day. Perceived physical activity barriers were lack of time, lack of social support, lack of energy, lack of willpower, and fear of falling. Results: In the group under 64 year, the lack of time (exp(B)=0.819, p=0.002) and the lack of willpower (exp(B)=0.656, p<0.001) were significantly associated. In the 65-74 year old group, the lack of willpower (exp(B)=0.714, p<0.001) and fear of falling (exp(B)=0.787, p=0.003) were a significant association. In the group aged 75 years and over, lack ofwill power (exp(B)=0.734, p<0.001) and fear of falling (exp(B)=0.807, p=0.003) were significantly associated. Conclusion: In order to solve the lack of willpower and lack of time, a walking activity should be performed together at a certain time in the village, and the elderly in the late ages should reduce the fear of falling by performing a fall prevention exercise in parallel.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.3
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• pp.94-102
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• 2010

Carbon Neutral is one of the magnifying pan-governmental tasks which aim for stabilizing pan-global ecosystem. The purpose of this study is to estimates carbon neutral degree in cities by using capacity of carbon storage, which is carbon sinks, on forest and to develop an indicator that could be utilized in establishing policy related to climate change respond. When the carbon emitted from city has been absorbed or stored, the value of the indicator aiming to achieve carbon neutral could be drawn. The result and implication are as follows. First, while the annual amount of carbon emission in Gyeonggi S city was 18,787 thousand (tons), which was the highest, that of Gyeongbuk U city indicated the lowest with approximately 112 thousand (tons). Second, Gyeongbuk U city represented the largest capacity of annual carbon storage with about 16,748 thousand (tons), whereas the smallest figure was shown in Gyeonggi B city with 151 thousand (tons). Third, as result of the estimation of carbon neutral degree, the value of the indicator in Gyeonggi B city was 3% referring the lowest point, while that of other cities demonstrated over 100%. Forth, it has the possibility to suggest the fixed quantity when the plan for achieving carbon neutral of city is being processed. In the near future, after the completion of the construction projects of greenhouse gas inventory in all local governments, by utilizing the relative dates, the value of the indicator can be calculated and a more general conclusion could be drawn. Moreover, as expanding case studies to all domestic cities, generalness is in need.

• Journal of Microbiology and Biotechnology
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• v.30 no.3
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• pp.417-426
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• 2020

Bacillus amyloliquefaciens is an important plant disease-preventing and growth-promoting microorganism. B. amyloliquefaciens WS-8 can stimulate plant growth and has strong antifungal properties. In this study, we sequenced the complete genome of B. amyloliquefaciens WS-8 by Pacific Biosciences RSII (PacBio) Single Molecule Real-Time (SMRT) sequencing. The genome consists of one chromosome (3,929,787 bp) and no additional plasmids. The main bacteriostatic substances were determined by genome, transcriptome, and mass spectrometry data. We thereby laid a theoretical foundation for the utilization of the strain. By genomic analysis, we identified 19 putative biosynthetic gene clusters for secondary metabolites, most of which are potentially involved in the biosynthesis of numerous bioactive metabolites, including difficidin, fengycin, and surfactin. Furthermore, a potential class II lanthipeptide biosynthetic gene cluster and genes that are involved in auxin biosynthesis were found. Through the analysis of transcriptome data, we found that the key bacteriostatic genes, as predicted in the genome, exhibited different levels of mRNA expression. Through metabolite isolation, purification, and exposure experiments, we found that a variety of metabolites of WS-8 exert an inhibitory effect on the necrotrophic fungus Botrytis cinerea, which causes gray mold; by mass spectrometry, we found that the main substances are mainly iturins and fengycins. Therefore, this strain has the potential to be utilized as an antifungal agent in agriculture.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.28 no.10
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• pp.779-787
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• 2017

In this paper, we designed a RF transceiver for small unmanned aerial vehicle(UAV) using power switching method. To apply for the UAV, several characteristics such as size, weight, and power consumption are very important. To reduce power consumption, we propose a new power switching method. Using the proposed method, we fabricated the RF transceiver needed to establish the data link for a small UAV. The fabricated RF transceiver shows an output power of +25 dBm, a noise figure of 4.56 dB and a received signal strength of -100 dBm. By performing power measurement of proposed switching method, 25 % of power could be reduced. The size of the fabricated RF transceiver is $100{\times}60{\times}5.7mm^3$ and the weight is as small as 38 g.

• Journal of the Korean Mathematical Society
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• v.36 no.4
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• pp.787-811
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• 1999

Let M be the maximal ideal space of the Banach algebra $H^{\infty}$ of bounded analytic functions on the open unit disc $\triangle$. For a positive singular measure ${\mu}\;on\;{\partial\triangle},\;let\;{L_{+}}^1(\mu)$ be the set of measures v with $0\;{\leq}\;{\nu}\;{\ll}\;{\mu}\;and\;{{\psi}_{\nu}}$ the associated singular inner functions. Let $R(\mu)\;and\;R_0(\mu)$ be the union sets of $\{$\mid$\psiv$\mid$\;<\;1\}\;and\;\{$\mid${\psi}_{\nu}$\mid$\;<\;0\}\;in\;M\;{\setminus}\;{\triangle},\;{\nu}\;\in\;{L_{+}}^1(\mu)$, respectively. It is proved that if $S(\mu)\;=\;{\partial\triangle}$, where $S(\mu)$ is the closed support set of $\mu$, then $R(\mu)\;=\;R0(\mu)\;=\;M{\setminus}({\triangle}\;{\cup}\;M(L^{\infty}(\partial\triangle)))$ is generated by $H^{\infty}\;and\;\overline{\psi_{\nu}},\;{\nu}\;{\in}\;{L_1}^{+}(\mu)$. It is proved that %d{\theta}(S(\mu))\;=\;0$ if and only if there exists as Blaschke product b with zeros $\{Zn\}_n$ such that $R(\mu)\;{\subset}\;{$\mid$b$\mid$\;<\;1}\;and\;S(\mu)$ coincides with the set of cluster points of $\{Zn\}_n$. While, we proved that $\mu$ is a sum of finitely many point measure such that $R(\mu)\;{\subset}\;\{$\mid${\psi}_{\lambda}$\mid$\;<\;1}\;and\;S(\lambda)\;=\;S(\mu)$. Also it is studied conditions on \mu for which $R(\mu)\;=\;R0(\mu)$.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.15 no.9
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• pp.783-787
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• 2002

In recent year, B $i_{4-}$x L $a_{x}$ $Ti_3$ $O_{12(BLT)}$ is one of promising substitute materials for the ferroelectric random access memory(FRAM) applications. But the systematic composition is still insufficient, so this experiment was carried out in ceramic ambient sintering process which has the very excellent ferroelectric property. Samples were prepared by a bulk and the purpose which was estimated with a suitability of thin films applications. The density of B $i_{3.75}$ L $a_{0.25}$ $Ti_3$ $O_{12}$ was high and the XRD pattern showed that the intensity of main peak (117) was increased at the argon ambient sintering. Controlling the quantity of oxygen, crystallization showed a thin, long plate like type, and we obtained the excellent dielectric and polarization properties at the argon atmosphere sintering. Also this sintering process was effective at the bulk sample. Argon ambient sintered sample produced higher permittivity of 154, the remanent polarization(2Pr) of 6.8 uC/$\textrm{cm}^2$ compared with that sintered in air and oxygen ambient. And this sintering process showed a possibility which could be applied to thin films process..