• Journal of the Korean institute of surface engineering
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• v.38 no.2
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• pp.60-64
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• 2005

The ICP-CVD (inductively coupled plasma chemical vapor deposition) process was applied to the deposition of $TiB_2$ films. For plasma generation, 13.56 MHz r.f. power was supplied to 2-turn Cu coil placed inside chamber. And the gas mixture of $TiCl_4,\;BCl_3,\;H_2$ and Ar was used for $TiB_2$ deposition. $TiB_2$ films with high hardness (<40 GPa) were obtained at extremely low deposition temperature $(250^{\circ}C)$, and the films hardness increased with ICP power and gas flow ratio of $TiCl_4/BCl_3$. The film structure was changed from (100) preferred orientation to random orientation with increasing RF power. It is supposed that the enhanced hardness of films was caused by a strong Ti-B chemical bonding of stoichiometric $TiB_2$ films and film densification induced by high density plasma.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.29 no.2
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• pp.56-64
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• 2016

Objectives : The purpose of this study is to observe the effects of Gardeniae Fuctus(GF) on the metabolic process of antioxidant and anti-inflammation. Methods : 4-HNE was injected into PC-12 cell to cause oxidative stress-induced inflammatory response, and then a western blot was conducted to observe the expression of Nuclear Factor-kB (NF-kB) and c-Jun N-terminal kinase (JNK) protein that are important factors involved with inflammation. Results : 1. The Gardeniae Fuctus water extract 50 ㎍ and 100 ㎍ significantly suppressed the increase in JNK protein expression in PC-12 cell. 2. The Gardeniae Fuctus water extract 50 ㎍, 100 ㎍ and 200 ㎍ significantly suppressed the increase in NF-kB protein expression in PC-12 cell. Conclusion : These results suggest that the Gardeniae Fuctus water extract has antioxidative and anti-inflammatory activity through suppressing the activity of JNK and NF-kB.

• Environmental Analysis Health and Toxicology
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• v.17 no.2
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• pp.175-185
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• 2002

To investigate effects of A. gmelini on the 14-day CCl$_4$induced hepatotoxicity, extracts were prepared in 3 ml saline at the dose of 50, 500, 5,000 mg/kg b.w. to administer orally everyday and same concentration (1 : 9, CCl$_4$: olive oil v/v) of CCl$_4$administered intraperitoneally with 2.5 ml/kg b.w. On the 7th day, hemanalysis showed following recovery values; AST 9.9∼64.6%, ALT 36.9∼71.9%, ALP 75.3∼93.7％, BUN 53.8∼59.7%, TBIL 60.4∼100.0< ％, TCHO 77.7∼100.0< ％, and TG 60.4∼100.0< ％. Even if, 14-day CCl$_4$induced hepatotoxicity recovery was found to depend on doses of extract, and recovery values of each treatment were AST 13.8∼56.4％, ALT 15.7∼68.0％, ALP 53.4∼84.4％, BUN 76.9∼100.0< ％, TBIL 60.4 ∼ 100.0< %, TCHO 82.6∼99.3< ％, and TG 56.7∼99.7%. By histological examination of liver, hydropic degeneration, fatty change, lipid accumulation and necrosis were also recovered.

• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.490-495
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• 1990

The extracellular inulinase from Bacillus spp. was purified to a single protein through a sequence of operations including ammonium sulfate fractionation, heat treatment, DEAE Sepharose C1-6B ion exchange chromatography, Sephadex 6-100 and Sephadex 6-150 gel filtration. The purified enzyme was confirmed to be a $\beta$ -D-fructofuranosidase(EC 3.2.1.26) which was much more active on sucrose than on inulin(I/S = 0.2). The maximal inulinase activity was observed at pH 6.0 and at the temperature of $50^{\circ}C$. The mo1ecular weight of the enzyme was about 56, 000. Tryptophan and histidine residues of the enzyme molecule were found to be essential for its catalytic activity.

• BMB Reports
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• v.28 no.4
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• pp.353-358
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• 1995

Epitope tagging is the process of fusing a set of amino acid residues that are recognized as an antigenic determinant to a protein of interest. Tagging a protein with an epitope facilitates various immunochemical analyses of the tagged protein with a specific monoclonal antibody. The monoclonal antibody H8 has subtype specificity for an epitope derived from the preS2 region of hepatitis B virus surface antigen. Previous studies on serial deletions of the preS2 region indicated that the preS2 epitope was located in amino acid residues 130~142. To test whether the amino acid sequence in this interval is sufficient to confer on proteins the antigenicity recognizable by the antibody H8, the set of amino acid residues in the interval was tagged to the amino terminal of ${\beta}$-galactosidase and to the carboxyl terminal of the truncated $p56^{lck}$ fragment. The tagged ${\beta}$-galactosidase, expressed in Escherichia coli, maintained the enzymatic activity and was immunoprecipitated efficiently with H8. The tagged $p56^{lck}$ fragment, synthesized in an in vitro translation system, was also immunoprecipitated specifically with H8. These results demonstrate that the amino acid sequence of the preS2 region can be used efficiently for the epitope tagging approach.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.413-419
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• 1998

Aryl acylamidase [EC 3.5.1.13] present in an acetaminophen-assimilating Pseudomonas sp. has been purified to a homogeneity using series of ammonium sulfate fractionation, DEAE-Sephacel anion exchange, Phenyl-Sepharose CL-4B hydrophobic, and Sephadex G-100 gel-permeation chromatography. The molecular weight, which was estimated by gel-permeation filtration and sodium dodecyl sulfate polyacylamide gel electrophoresis, was about 57 kDa and 56 kDa, respectively, indicating that this enzyme is a monomeric protein. The optimum pH was 10.5 and the optimum temperature was 40$^{\circ}C$. After incubation of the enzyme at 50$^{\circ}C$ for 30 min, residual activity of the enzyme was 34% compared to its original activity. The Km values for acetaminophen and 4'-nitroactanilide were 0.10 mM and 0.11 mM, respectively.

• Nutritional Sciences
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• v.2 no.2
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• pp.102-106
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• 1999

Changes in nutritional and health status brought on by a two-year balanced diet were assessed with anthropometric measurements as well as hematological and lipid profiles in 56 healthy young men. Recommended dietary allowances (RDA) were adjusted with estimated daily weighted activity factor. The weighted resting energy expenditure factor of the subjects was 2.37 $\pm$ 0.05. Compared with RDA, all nutrient intakes were adequate and 56% of energy was supplied by carbohydrates, 18% by protein and 26% by fat. The vitamin and mineral intakes except vitamin B-2 were higher (26.46-129.88%) than RDA. Vitamin B-2 intake was 92.15 \ulcorner 14.16% of RDA. There was no seasonal variation on nutrient intakes. Height was increased and systolic blood pressure was decreased by balanced diet for two years. Body weight, diastolic blood pressure and body mass index (BMI) were unchanged. The level of hemoglobin and hematocrit was not changed, the level of plasma protein was decreased and the level of plasma total cholesterol and albumin was increased. These results suggest that a balanced diet can increase height and complement health status achieved through vigorous exercise, even in adults, and that the level of Korean RDA for energy is adequate to maintain existing body weight.

• Microbiology and Biotechnology Letters
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• v.19 no.4
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• pp.372-379
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• 1991

A rapid and simple quantitative assay method for aminoglycoside-3'- phosphotransferase (APH(3')) derived from E. coli ATCC 21990 was developed using the thin layer chromatographic densitometry, 3'-phosphorylated kanamycin B (3'-PKMB), product of APH (3') reaction, was separated from reaction mixtures by developing on the silica gel TLC plate with chloroform-methanol-ammonia water (3:4:3). The quantity of the 3'-PKMB was measured by densitometry after color development by ninhydrin method. Densitometric TLC assay for APH (3') was showed a good quantitative result and reproducibility. Sensitivity of this assay was 1.56 nmol of 3'-PKMB and could be analyzed many samples at same time. This method may be applicable for the analysis of inactivating enzymes of aminoglycoside antibiotics.

• Journal of Chest Surgery
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• v.45 no.1
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• pp.56-59
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• 2012

Aspergillus is a ubiquitous fungus and can cause many levels of disease severity. Chronic necrotizing aspergillosis is a rare disease and few cases have been reported in Korea. We experienced a case of pleural aspergillosis that was treated successfully with medical and surgical interventions. The 52-year-old man who was diagnosed with chronic necrotizing pulmonary aspergillosis underwent surgical treatment including a lobectomy, decortication, and myoplasty. The patient was also medically treated with amphotericin B followed by voriconazole. Pleural irrigation with amphotericin B was also performed. A multi-dimensional approach should be considered for treating chronic necrotizing pulmonary aspergillosis.

• Journal of the Korean Ceramic Society
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• v.56 no.2
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• pp.173-177
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• 2019

Thermal properties of a SiO₂-B₂O₃-BaO ternary glass system depending on compositional change of BaO have been examined to find a proper sealing material for reversible oxide cells. Glass transition temperature and thermal expansion coefficients increased simultaneously up to 55 mol% of BaO content. The structural role of BaO with regard to the thermal properties has been discussed on the basis of Raman spectroscopy results. Flowability of the glass at sealing temperature has been examined with packed glass powders of 12 mm diameter along with a high temperature optical microscope. The practical sealing property of the glass was also examined with YSZ coated with NiO-yittria stabilized zirconia (NiO-YSZ) and it showed good adhesion without noticeable reaction with NiO-YSZ layer.