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http://dx.doi.org/10.6114/jkood.2016.29.2.056

Effects of Gardeniae Fructus on the metabolic process of antioxidant and anti-inflammation by JNK and NF-kB  

Lee, Sang-Chul (Dept. of Anatomy-Pointology, College of Korean Medicine, Gachon University)
Sim, Sung-Yong (Dept. of Ophthalmology & Otorhinolaryngology & Dermatology, College of Korean Medicine, Gachon University)
Kim, Youn-Sub (Dept. of Anatomy-Pointology, College of Korean Medicine, Gachon University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.29, no.2, 2016 , pp. 56-64 More about this Journal
Abstract
Objectives : The purpose of this study is to observe the effects of Gardeniae Fuctus(GF) on the metabolic process of antioxidant and anti-inflammation. Methods : 4-HNE was injected into PC-12 cell to cause oxidative stress-induced inflammatory response, and then a western blot was conducted to observe the expression of Nuclear Factor-kB (NF-kB) and c-Jun N-terminal kinase (JNK) protein that are important factors involved with inflammation. Results : 1. The Gardeniae Fuctus water extract 50 ㎍ and 100 ㎍ significantly suppressed the increase in JNK protein expression in PC-12 cell. 2. The Gardeniae Fuctus water extract 50 ㎍, 100 ㎍ and 200 ㎍ significantly suppressed the increase in NF-kB protein expression in PC-12 cell. Conclusion : These results suggest that the Gardeniae Fuctus water extract has antioxidative and anti-inflammatory activity through suppressing the activity of JNK and NF-kB.
Keywords
Gardeniae Fuctus; 4-HNE; JNK; NF-kB;
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