• Title/Summary/Keyword: B56

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Polymerase chain reaction for the detection of Toxoplasma gondii in the blood of cats (중합효소연쇄반응(PCR)을 이용한 고양이 혈액내에서의 Toxoplasma gondii 검출에 관한 연구)

  • Suh, Myung-deuk;Joo, Bo-hyun
    • Korean Journal of Veterinary Research
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    • v.39 no.6
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    • pp.1151-1160
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    • 1999
  • This study was conducted to detect the toxoplasma-specific DNA in peripheral blood collected from cats experimentally infected with Toxoplasma gondii (RH strain) and from domiciled cats by B1 gene-base polymerise chain reaction(PCR). The sensitivity of oligonucleotide primer, T-1 & T-2, designed from toxoplasma B1 gene amplification method was compared with parasite detection by mouse inoculation(MI). And also, latex agglutination test(LAT) and indirect fluorescent antibody test(IFAT) were conducted to detect the fluctuation of serum antibodies compared with the detection of toxoplasma by PCR and MI. Toxoplasma B1 gene PCR was shown consistently high sensitivity and the results obtained by PCR agreed completely with those from MI. All blood samples collected before infection with T gondii gave negative results by PCR and MI. Also, toxoplasma Bl gene PCR was not cross reaction with Neospora caninum DNA and normal cat leucocyte as controls. The toxoplasma-specific DNA was detected by PCR in blood of 5 cats experimentally infected with T gondii 6 days after infection and the detection of this specific-DNA was long lasted in blood for 64 days after infection. The detection of toxoplasma-specific DNA by PCR could be identified as few as 10 tachyzoites and the isolation of T gondii by MI could be isolated as few as 1 tachyzoite from tenfold serial dilution of T gondii with normal cat blood, respectively. In healthy domiciled cats, the toxoplasma-specific DNA and the parasite were detected and isolated in blood from 3 of 56(5.3%) cats by both PCR and MI, respectively. In the results of antibody test from the total 56 heads of healthy domiciled cats, the positive rates are 15(26.7%) by LAT and 19(33.9%) by IFAT. These results suggest that PCR detection of toxoplasma can be applied as a sensitive and specific diagnostic and research tool.

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Structure of Cholesteryl Pentyl Carbonate (Cholesteryl Pentyl Carbonate의 결정 및 분자구조)

  • Seo, Hye Ran;Park, Yeong Ja;B. M. Craven
    • Journal of the Korean Chemical Society
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    • v.34 no.1
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    • pp.29-36
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    • 1990
  • Cholesteryl pentyl carbonate $(C_{33}H_{56}O_3)$ is monoclinic, space group P21, with a = 12.484(3), b = 9.043(3), c = 14.053(3)$\AA$, ${\beta} = 94.12(2)^{\circ}$ and z = 2. The intensity data were measured for the 2969 reflections within sin $\theta/\lambda = 0.52 {\AA}^{-1}$, using an automatic four--circle diffractometer and graphite monochromated Mo-K$\alpha$ radiation. The atomic coordinates from cholesteryl octanoate were used in an initial trial structure and the structure was refined by full-matrix least squares methods. The final R-factor was 0.12 for 1164 observed reflections. The pentyl group has shortened bond lengths due to the high thermal vibrations in this region. Adjacent molecules are related by $2_1$ screw axis so that they are arranged in an antiparallel array, corresponding to the Monolayer Type II packing mode. There are close packings of cholesteryl groups within the monolayers. This packing type is similar to those of cholesteryl hexanoate, octanoate, hexyl carbonate and oleate.

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On the Numbers of Palindromes

  • Bang, Sejeong;Feng, Yan-Quan;Lee, Jaeun
    • Kyungpook Mathematical Journal
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    • v.56 no.2
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    • pp.349-355
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    • 2016
  • For any integer $n{\geq}2$, each palindrome of n induces a circulant graph of order n. It is known that for each integer $n{\geq}2$, there is a one-to-one correspondence between the set of (resp. aperiodic) palindromes of n and the set of (resp. connected) circulant graphs of order n (cf. [2]). This bijection gives a one-to-one correspondence of the palindromes ${\sigma}$ with $gcd({\sigma})=1$ to the connected circulant graphs. It was also shown that the number of palindromes ${\sigma}$ of n with $gcd({\sigma})=1$ is the same number of aperiodic palindromes of n. Let $a_n$ (resp. $b_n$) be the number of aperiodic palindromes ${\sigma}$ of n with $gcd({\sigma})=1$ (resp. $gcd({\sigma}){\neq}1$). Let $c_n$ (resp. $d_n$) be the number of periodic palindromes ${\sigma}$ of n with $gcd({\sigma})=1$ (resp. $gcd({\sigma}){\neq}1$). In this paper, we calculate the numbers $a_n$, $b_n$, $c_n$, $d_n$ in two ways. In Theorem 2.3, we $n_d$ recurrence relations for $a_n$, $b_n$, $c_n$, $d_n$ in terms of $a_d$ for $d{\mid}n$ and $d{\neq}n$. Afterwards, we nd formulae for $a_n$, $b_n$, $c_n$, $d_n$ explicitly in Theorem 2.5.

Effect of Carbon-based Nanofillers on the Toughening Behavior of Epoxy Resin

  • Lee, Gi-Bbeum;Kim, Haeran;Shin, Wonjae;Jeon, Jinseok;Park, In-Seok;Nah, Changwoon
    • Elastomers and Composites
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    • v.56 no.3
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    • pp.179-186
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    • 2021
  • Carbon-based nanofillers, including nanodiamond (ND) and carbon nanotubes (CNTs), have been employed in epoxy matrixes for improving the toughness, using the tow prepreg method, of epoxy compounds for high pressure tanks. The reinforcing performance was compared with those of commercially available toughening fillers, including carboxyl-terminated butadiene acrylonitrile (CTBN) and block copolymers, such as poly(methyl methacrylate)-b-poly(butyl acrylate)-b-poly(methyl methacrylate) (BA-b-MMA). CTNB improved the mechanical performance at a relatively high filler loading of ~5 phr. Nanosized BA-b-MMA showed improved performance at a lower filler loading of ~2 phr. However, the mechanical properties deteriorated at a higher loading of ~5 phr because of the formation of larger aggregates. ND showed no significant improvement in mechanical properties because of aggregate formation. In contrast, surface-treated ND with epoxidized hydroxyl-terminated polybutadiene considerably improved the mechanical properties, notably the impact strength, because of more uniform dispersion of particles in the epoxy matrix. CNTs noticeably improved the flexural strength and impact strength at a filler loading of 0.5 phr. However, the improvements were lost with further addition of fillers because of CNT aggregation.

Analysis of Algal Bloom Occurrence Characteristics Namyang Lake using Sentinel-2 MSI (Sentinel-2 MSI를 활용한 남양 간척담수호의 조류발생 특성 분석)

  • Wonjin Jang;Jinuk Kim;Jiwan Lee;Yongeun Park;Seongjoon Kim
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.56-56
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    • 2023
  • 남양호는 농업용수 공급을 위해 건설된 하구 담수호로 과도한 영양물질 축적으로 인해 매년 여름 녹조류가 번성한다. 따라서 본 연구에서는 조류발생 특성을 분석하고자 식물성 플랑크톤 및 관련 분해 산물에 의해 고유 광학특성을 가지고 있는 Chlorophyll-a(Chl-a)의 추정을 통한 녹조 발생을 파악하고자 Sentinel-2 Multi Spectral Image(MSI)의 원격 반사율 광학 스펙트럼을 사용하였다. Chl-a 추정알고리즘 개발을 위하여 Sentinel-2 A, B의 교차 방문주기인 5일 간격에 맞추어 현장수질자료(2022년: 27회 2023년: 27회)를 측정하였다. Chl-a 농도는 EXO-YSI를이용하여 측정하였으며 해당기간동안 9.4 ~ 127.1 mg/L의 범위를 보였으며, Sentine-2 자료는 A, B자료에서 B1(443 nm) ~ B8A(865 nm)파장의 값을 기상조건(구름, 안개, 강수)을 고려하여 현장수질측정 위치에서 반사도를 추출하였다. 입력자료는 대기 및 방사영향을 고려해 반사도 간의 비율자료와 선행연구에서 활용된 반사도를 활용하였으며 알고리즘은 다중선형회귀분석(Multi Linear Regression Model)과 Random Forest를 활용하였다. MLR의 경우 결정계수(R2)가 학습 및 검증에서 각각 0.68, 0.59의 성능을 보였으며, RF의 경우 각각 0.94, 0.85의 성능을 보였다. 해당알고리즘으로 생성된 Chl-a 시공간농도 자료는 담수호내 조류발생 특성을 분석하고 효율적 조류관리 및 대처에 활용될 것으로 판단된다.

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Effects of Dietary Prebiotic, Probiotics and Synbiotic on Growth, Nonspecific Immunity, Antioxidant Capacity, Intestinal Microbiota and Antiinflammatory Activity of Hybrid Grouper (Epinephelus akaara ♀×Epinephelus lanceolatus ♂) (사료 내 Prebiotic, Probiotics와 Synbiotic의 첨가가 대왕붉바리(Epinephelus akaara ♀×Epinephelus lanceolatus ♂)의 성장, 비특이적 면역력, 항산화능, 장내 미생물 조성과 항염증에 미치는 영향)

  • Wonhoon Kim;Jongho Lim;Minjoo Kang;Choong Hwan Noh;Kyeong-Jun Lee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.6
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    • pp.850-860
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    • 2023
  • The effects of dietary mannan oligosaccharides, Lactobacillus plantarum, Bacillus subtilis, and Bacillus licheniformis supplementation on hybrid grouper Epinephelus akaara ♀×Epinephelus lanceolatus ♂ were evaluated. The fish were fed a basal diet and five other diets consisting of 0.6% mannan oligosaccharides, L. plantarum, B. subtilis, and B. licheniformis and mixture of each 0.15% prebiotic and all the probiotics (designated as MOS, LP, BS, BL, and SYN) for 56 days. Growth performance and feed utilization showed no significant differences among all experimental groups. Lipid level of whole-body was significantly high in MOS and BL groups. Plasma aspartate aminotransferase was significantly low in BL and SYN groups. Nitro-blue tetrazolium, lysozyme and anti-protease, and glutathione peroxidase in BS, SYN, and all probiotic groups, respectively, were significantly high. Intestinal Vibrio bacteria was significantly low in all probiotic and SYN groups. Gene expression of interleukin-1β and interleukin-10 in SYN group; transforming growth factor β2 in MOS and BS groups, toll-like receptor 2-2 in BS and BL groups; and C-type lectin in MOS, LP and SYN groups were significantly upregulated. Our findings indicate that mannan oligosaccharides, L. plantarum, B. subtilis, and B. licheniformis could improve innate immunity, antioxidant capacity, anti-inflammation, and intestinal microbiota of hybrid grouper.

미국의 대형 기술개발 현황(상)

  • Korea Defense Industry Association
    • Defense and Technology
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    • no.2 s.72
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    • pp.48-56
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    • 1985
  • 미국의 산업계, 학계, 정부기관은 서로 협력해서 아주 대규모의 기술개발을 정력적으로 추진하고 있다. 금번 미연구우주국국(NASA), 국방성, 각지의 대학, 연구소등 미국의 대형 기술개발의 "최전선" 을 취재해서 (1)우주개발, (2)빔병기와 군사기술, (3)핵융합, (4)차조전략폭격기 B1 의 4개항으로 나누어 그 동향을 살펴보았다 .

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