• Journal of Acupuncture Research
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• v.22 no.3
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• pp.155-167
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• 2005

Objectives : The purpose of this study was to investigate the anti-inflammatory effect of Cobrotoxin on binding affinity of cobrotoxin with P50, $IKK{\alpa}$ and $IKK{\beta}$, activities of NF-${\kappa}B$, Cell viability of astrocyte, expressions of protein molecules of NF-${\kappa}B$ such as P50, P-$1{kappa}B$, $1{\kappa}B$ and iflammation related genes such as Cox-2, iNOS, cPLA2 in the SNP or LPS induced Inflammatory pathway of Rats' astrocytes. Methods : In this study, The expression of cytosolic phospholipase A2, Nitric oxcide, Cyclooxygenase-2 and inducible nitrogen oxide synthase was determined by western blotting with corresponding antibodies, and the generation of NF-${\kappa}B$ was assayed by EMSA method in astrocytes of rats. The Cell viability of astrocytes was determined by MTT assay, and Binding affinity of Cobrotoxin with P50, $IKK{\alpha}$ and $IKK{\beta}$ was assayed by Surface plasmon resonance analysis, and NF-${\kappa}B$ dependent luciferase activity was determined by luciferase analysis, and Uptake of cobrotoxin in astrocytes was identified by Confocal laser scanning microscope Results : 1. Compared with control, LPS-induced NF-${\kappa}B$ DNA binding activity was decreased significantly by 0.1, $0.5{\mu}g/m{\ell}$ of Cobrotoxin in Astrocyte. 2. Compared with control, LPS-induced NF-kB dependent luciferase expression was decreased significantly by 0.1, 0.5 and $1{\mu}g/m{\ell}$ of Cobrotoxin in Astrocyte. 3. Compared with control, SNP induced P50, $I{\kappa}B$ expressions in astrocyte were decreased significantly by 0.1, 0.5 and $1{\mu}g/m{\ell}$ of Cobrotoxin and P-$1{\kappa}B$ expression was decreased significantly by 0.5 and $1{\mu}g/m{\ell}$ of Cobrotoxin. 4. Compared with control, LPS induced P50, $1{\kappa}B$ expressions in astrocyte were decreased significantly by 0.5 and $1{\mu}g/m{\ell}$ of Cobrotoxin. 5. Compared with control, SNP induced Cox-2, iNOS, CPLA2 expressions in astrocyte were decreased significantly by $1{\mu}g/m{\ell}$ of Cobrotoxin. 6. Compared with control, LPS induced Cox-2, cPLA2 expressions in astrocyte were decreased significantly by 0.1, 0.5, $1{\mu}g/m{\ell}$ of Cobrotoxin and iNOS expression was decreased significantly by 0.5, $1{\mu}g/m{\ell}$ of Cobrotoxin. 7. Compared with $0.5{\mu}g/m{\ell}$ of Cobrotoxin, SNP-induced NF-${\kappa}B$ DNA bindins activity in astrocyte was increased significantly by Cobrotoxin $0.5{\mu}g/m{\ell}$ with DTT 1mM and Cobrotoxin $0.5{\mu}g/m{\ell}$ with DTT 5mM. 8. Compared with $0.5{\mu}g/m{\ell}$ of Cobrotoxin, LPS-induced NF-${\kappa}B$ DNA binding activity in astrocyte was increased significantly by Cobrotoxin $0.5{\mu}g/m{\ell}$ with DTT 1mM, Cobrotoxin $0.5{\mu}g/m{\ell}$ with DTT 5mM, Cobrotoxin $0.5{\mu}g/m{\ell}$with GSH 1mM and Cobrotoxin $0.5{\mu}g/m{\ell}$ with GSH 5mM 9. Compared with $0.1{\mu}g/m{\ell}$ of cobrotoxin, SNP induced P50 expressions in astrocyte were increased significantly by Cobrotoxin $0.5{\mu}g/m{\ell}$ with DTT 1mM, Cobrotoxin $0.5{\mu}g/m{\ell}$ with DTT 5mM Cobrotoxin $0.5{\mu}g/m{\ell}$ with GSH 1mM and Cobrotoxin $0.5{\mu}g/m{\ell}$ with GSH 5mM. 10. The uptake of the labeled cobrotoxin into the cells was shown under a confocal laser scanning microscope. cobrotoxin was uptaken into the membrane and nucleus of astrocytes. Conclusions : In summary, the present results demonstrate that cobrotoxin directly binds to sulfhydryl group of p50 and IKKS resulting In the reduction of translocation of p50 and IkB release, thereby inhibits activation of NF-${\kappa}B$, and suggest that pico to nanomolar range of cobrotoxin could inhibit the expression of genes in the NF-${\kappa}B$ signal pathway.

• Journal of Nutrition and Health
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• v.45 no.6
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• pp.588-599
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• 2012

There is a limitation to estimate vitamin $B_{12}$ intake due to the lack of data on vitamin $B_{12}$ content of Korean commercial foods. In this study, vitamin $B_{12}$ content was determined in favorite Korean restaurant foods, convenient or instant foods, fast foods and bakery products through a modified microbioassay using Lactobacillus delbrueckii ATCC 7830. Bulgogi and seafood & green pepper griddle had high vitamin $B_{12}$ content, 3.50 and $2.96{\mu}g$/100 g, respectively. Pork suyook, pork griddle and pollack griddle had 0.48, 0.31 and $0.32{\mu}g$/100 g of vitamin $B_{12}$, respectively. In stew, soft-tofu stew with seafood and doenjang stew with seafood had relatively high vitamin $B_{12}$ content, 1.93 and $1.44{\mu}g$/100 g, respectively. Bibimbap and 4 different types of rice porridge, beef & mushroom, chicken & ginseng, seafood or abalone, had 0.36, 0.08, 0.09, 1.64 and $0.13{\mu}g$/100 g of vitamin $B_{12}$, respectively. One serving of haejanggguk, yookejang, chuotang and galbitang had 5.97, 2.04, 2.63 and $1.91{\mu}g$ of vitamin $B_{12}$, respectively. One serving of samgetang and sulongtang had $2.89{\mu}g$ and $6.64{\mu}g$ of vitamin $B_{12}$. In noodles, one serving of cram noodle soup, bibim-nangmyeon, and mul-nangmyeon had 18.8, 1.21 and $0.38{\mu}g$ of vitamin $B_{12}$, respectively. One regular gimbap and one triangle gimbap contained 1.09-2.53 and $0.54-1.11{\mu}g$ of vitamin $B_{12}$, respectively. One cheese-burger, chicken-burger and bulgogi-burger had 0.76, 0.62 and $0.54{\mu}g$ of vitamin $B_{12}$, respectively. A plain bagel and a waffle contained 0.13 and $0.17{\mu}g$/100 g of vitamin $B_{12}$, respectively. Ready-made tomato sauce or cream sauce for spaghetti in a retort pouch contained only a trace of vitamin $B_{12}$. In conclusion, these results should contribute to improving the present food vitamin $B_{12}$ content database, most of which were cited from foreign data, thereby it could be helpful to estimate the vitamin $B_{12}$ intake of Koreans more accurately than before. It will also provide new information for dietary education related to vitamin $B_{12}$ and health.

• Korean Journal of Animal Reproduction
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• v.22 no.3
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• pp.237-244
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• 1998

To investigate the fidelity of transgene transmission and expression, we produced transgenic mice carrying bovine $\beta$-casein/bovine grwoth hormone(bGH) fusion gene and examined transmission efficiency and expression level of the transgene in the founders and their progeny. The transgene was composed of 1.8 kb bovine $\beta$-casein promoter and 2.1 kb bGH gene. Ten transgenic mice were produced. Milk and mammary gland were collected from eight transgenic lines at 10-day lactation and a, pp.ied to Western and Northern blot analyses. The bGH expression was detected in four of them. The concentrations of bGH in milk were highly variable from 4$\mu\textrm{g}$/ml to 600$\mu\textrm{g}$/ml depending on the lines. The bGH mRNA level in mammary gland was closely correlated with the bGH concentration in milk in each transgenic line. These results indicated that bGH transgene expression was a, pp.opriately regulated in the mammary gland and secreted into milk in transgenic mice. By using two transgenic lines(#2, #7) secreting a considerable amoung of bGH into their milk, the inferitance and maintenance of transgenic phenotype were assessed in successive four generations. The mean transmission frequencies of transgene in lines #2 and #7 were 34% and 40%, respectively. The bGH concentration in milk were 80, 240, 120, 60$\mu\textrm{g}$/ml in each G0(generation 0), G2, G3, G4 generation of line #2 and 600, 1600, 860, 900$\mu\textrm{g}$/ml in each G1. G2, G3, G4 generation of line #7. These results demonstrated that bovine $\beta$-casein/bGH gene was stably transmitted from generation to generation in a Menelian fashion in trasgenic mice and consistenly expressed in their milk throughout the generations, although there was a little variation in the transmission frequency and expression level of the transgene between generations.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.73-73
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• 2018

최근 매실나무를 기주로 한 겨우살이의 인공재배에 성공함에 따라, 식품 소재로 이용하기 위해 일반성분을 분석하였다. 경기도 남양주시에서 매실나무를 기주로 한 인공재배 겨우살이를 채취하여 동결건조한 후 수분, 조단백질, 조지방, 회분, 탄수화물, 베타카로틴, 비타민 B1, 비타민 B2, 비타민 B3, 비타민C, Ca, Fe, K, Na 그리고 P의 함량을 분석하였다. 조단백질, 조지방, 회분, 베타카로틴, Ca, Fe, K, Na 그리고 P은 AOAC 방법을 이용하여 분석하였고, 탄수화물은 탄수화물(%) = 100 - (조단백질% + 조지방% + 회분% + 수분%) 계산식을 활용하여 산출하였다. 수분, 비타민 B1, 비타민 B2, 비타민 B3, 비타민 C는 식품공전에 제시된 방법에 따라 분석하였다. 동결건조한 매실나무겨우살이의 일반성분은 탄수화물 58.90%, 단백질 22.36%, 회분 11.11%, 지방 5.37%, 수분 2.26% 순으로 함유하였다. 비타민의 함량은 비타민 C 4.78mg/100g, 나이아신 1.87mg/100g, 비타민 A 1.42mg/100g, 비타민 B2 0.73mg/100g 그리고 비타민 B1 0.30 mg/100g의 순서로 많이 함유하였다. 무기질은 K 3141.54mg/100g, Ca 977.77mg/100g, P 309.91 mg/100g, Na 20.13mg/100g 그리고 Fe 8,24mg/100g의 순서로 많이 함유하였다.

• The Korean Journal of Mycology
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• v.41 no.2
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• pp.118-126
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• 2013

The experiment was carried out to analyze the inhibition effect of plant pathogenic fungi and growth promotion activity induced by the bacterial strains isolated from peatmoss. Among the isolated bacterial strains, B10-2, B10-4, B10-5 and B10-6 which showed more than 30% inhibition rate against Botrytis cinerea and Rhizoctonia solani in vitro, were further analyzed in the greenhouse for the growth promotion activity on lettuce (Lactuca sativa), pak-choi (Brassica compestris L. ssp. chinensis) and Chinese cabbage (Brassica campestris L. ssp. pekinensis). The results showed the treatment of B10-4 on lettuce showed the highest growth promotion activity with the leaf area ($169.17cm^2$), fresh weight (leaf: 40.29 g, root: 8.80 g)and dry weight (leaf: 11.24 g, root: 4.17 g), which was about two folds as compared to control. On pak-choi, the growth promotion rate was the highest with the leaf area of $112.87cm^2$, leaf fresh weight of 60.70 g, root fresh weight of 3.37 g, leaf dry weight of 14.34 g, and root dry weight of 1.90 g. As a result of treatment of B10-13 on chinese cabbage, the growth promotion rate was the highest with the leaf area ($293.56cm^2$), fresh weight (leaf: 113.67 g, root: 2.40 g) and dry weight (leaf: 6.03 g, root: 0.53 g). The production of Indole Acetic Acid (IAA) and Indole-3-Butylic Acid (IBA) were also analyzed in these bacterial isolates. The IAA and IBA analyses were carried out in all bacterial isolates each day within the 5 days of incubation period. The highest production of IAA was observed with $112.57{\mu}g/mg$ protein in B10-4 after 3 days of incubation and IBA production was the highest in B10-2 with $58.71{\mu}g/mg$ protein after 2 days of incubation. Also, phosphate solubilizing activity was expressed significantly in B10-13 in comparison to that of other bacterial isolates. Bacterial identification showed that B10-2 was Bacillaceae bacterium and B10-5 was Bacillus cereus, B10-4 and B10-6 were Bacillus sp. and B-13 was Staphylococcus sp. by ITS sequence.

• The Korean Journal of Food And Nutrition
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• v.32 no.5
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• pp.565-570
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• 2019

Proteasome inhibitors can improve the efficiency of cancer treatments by inhibiting nuclear factor ${\kappa}B$($NF-{\kappa}B$) activation in cancer cells. Lentils are a type of beans of which consumption of such beans is increasing. The purpose of this study was to investigate the effects of lentils extract (LE) on the proteasomal activities, $NF-{\kappa}B$ activation, and cell cycle in HepG2 human liver cancer cells. LE treatments inhibited proteasomal activities at concentrations of 10, 50, and $100{\mu}g/mL$ respectively, and repressed $NF-{\kappa}B$ activation at concentrations of 1, 10, and $100{\mu}g/mL$ respectively, in HepG2 cells. LE treatments at concentrations of 1, 10, and $100{\mu}g/mL$ respectively, increased sub-G1 cell population in HepG2 cells, which may be the result of apoptosis. The results suggest that LE inhibited $NF-{\kappa}B$ activation partially with its proteasome inhibitory activities, and the increase of sub-G1 cell population was induced partially, by inhibition of $NF-{\kappa}B$ activation in HepG2 cells.

• Bulletin of the Korean Mathematical Society
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• v.30 no.2
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• pp.213-221
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• 1993

In this paper, we will show that the multiplicative group G in a finite ring R with identity 1 has a (B, N)-pair satisfying the following conditions; (1) G=BNB where B and N are subgroups of G. (2) B.cap.N is a normal subgroup of N and W = N/(B.cap.N), is generated by a set S = { $s_{1}$, $s_{2}$, .., $s_{k}$} where $s_{i}$.mem.N/(B.cap.N), $s_{i}$$^{2}$.iden.1 and $s_{i}$.neq.1. (3) For any s.mem.S and w.mem.W, we have sBw.contnd.BwB.cup.BswB. (4) We have sBs not .subeq. B for any s.mem.S. When G, B, N and S satisfy the above conditions, we say that the quadruple (G, B, N, S) is a Tits system. The group W is called the Weyl gorup of the Tits system.ystem.m.

• Journal of the Korean Society of Food Science and Nutrition
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• v.8 no.1
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• pp.9-14
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• 1979

The consumption of catchup is increasing due to the raising of income level and food industry and westernization of dietary life followed by economic growth. In this paper I picked up three kinds of food produced by two foreign food companies and three kinds of food by two domestic companies. The average results by experimentation on the nutritive ingredients and the standard quality was as follows; A. Nutritve ingredients; 1) Water content a) Domestic 68.3% b) Foreign 69.1% 2) Protein content a) Domestic 2.2 g b) Foreign 2.1 g 3) Fat content a) Domestic 0.1 g b) Foreign 0.1 g 4) Carbohydrate content a) Domestic 25.1 g b) Foreign 24.6 g 5) Mineral content (Ash) a) Domestic 4.4 g b) Foreign 3.9 g 6) Calcium content a) Domestic 33.5 mg b) Foreign 24.2 mg 7) Phosphorus content a) Domestic 16.1 mg b) Foreign 24.2 mg 8) Vitamin C content a) Domestic 14.6mg b) Foreign 16.0 mg B. Standard quality 1) Remains after evaporation a) Domestic 41.7% b) Foreign 38.4% 2) Free mineral acid content a) Domestic none b) Foreign none 3) Tar chromatophore a) Domestic $trace(Acid)^+$ b) Foreign none 4) Heavy metalic $elements^*$ 5) Sodium chloride content a) Domestic 3.3% b) Foreign 3.3% 6) pH level a) Domestic 3.83 b) Foreign 3.76 + The tar chromatophore elements could not be accurately measured by chromatography. * The heavy metalic elements were both under safety levels in the domestic and foreign products.

• Journal of Environmental Health Sciences
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• v.18 no.2
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• pp.52-56
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• 1992

The ability of chemicals, 10% sodium hypochlorite, 28% ammonium hydroxide, 5% sodium hydroxide, 5% sodium bicarbonate, 0.1% hydrochloric acid, 5% hydrogen peroxide, and 5% acetone, to destroy aflatoxins in laboratory waste water containing 3.26 ppb of B$_{1}$ 7.64 ppb of B$_{6}$3 ppb of G$_{1}$, and 11.39 ppb of G$_{2}$ with the total of 29.11 ppb was investigated. High performance liquid chromatograph (HPLC) was used for the separation and quantitation of aflatoxins. Treatment for 2 hours by the chemicals affected the destruction of aflatoxins and the most effective chemical was 10% sodium hypochlorite (p<0.05). Sodium hypochlorite concentrations more than 1% significantly reduced aflatoxin B$_{2}$, G$_{1}$, G$_{2}$ and total aflatoxins and more than 3% reduced B$_{1}$ (p<0.05). No further significant decreases were observed above the concentration of 5% for all 4 aflatoxins. Complete destruction of aflatoxins B$_{2}$, G_{1}$, and G$_{2}$ was achieved by 5% sodium hypochlorite at 48 hours and B$_{1}$ at 72 hours.

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.211-216
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• 2001

The growth of Selenastrum capricornutum in culture was investigated as a function of ionic copper concentrations to verify the toxicity and physiological variation. In a $Cu^{++}$ excess culture (E: $Cu^{++}$ of $130{\mu}g/l$), the growth rate (K)(0.32) was lower than that of the control culture (C: $Cu^{++}$ of $0.065{\mu}g/l$) (0.61) after 8 days and the exponential growth rate ($R_E$) was also found to be lower in culture E (1.1) than culture C (2.9). On the contrary, the K of S. capricornutum in trace $Cu^{++}$ culture ($T_1$ and $T_2$, 0.72) after 6 days tended to be more increased than culture C (0.68). From 8 to 14 days of culture, the amounts of chlorophyllls a and b were increased in culture C (chlorophyll a, $106->126{\mu}g/g$ dry wt; chlorophyll b, $158->208{\mu}g/g$ dry wt; chlorophyll b were decreased in culture E (chlorophyll a, $309->235{\mu}g/g$ dry wt; chlorophyll b, $405->352{\mu}g/g$ dry wt). The amounts of chlorophylls in ionic copper trace culture ($T_1$ and $T_2$) [(chl a/b) of $T_1$: $384/620\;{\mu}g/g$ dry wt; (chl a/b) of $T^2$:$320/467{\mu}g/g$ dry wt] were increased more than the culture C($260/387{\mu}g/g$ dry wt). Howeveer, when photosynthetic rates were normalized to the dry weight of algae, the control culture continued to showed higher values than the treated culture ($T_1$). An appropriate amount of ionic copper ($T_1:\;26\;{\mu}g/l$) stimulated the grwoth of S. capricornutum than the ionic copper content of $13\;{\mu}g/l$ ($T_2$), while the excess amount of ionic copper ($130\;{\mu}g/l$) resulted in the highest toxicity to the growth of S. capricornutum.