Browse > Article
http://dx.doi.org/10.4489/KJM.2013.41.2.118

Inhibition Effects Against Plant Pathogenic Fungi and Plant Growth Promotion by Beneficial Microorganisms  

Jung, Jin Hee (Department of Applied Plant Sciences, Kangwon National University)
Kim, Sang Woo (Department of Applied Plant Sciences, Kangwon National University)
Kim, Yun Seok (Department of Applied Plant Sciences, Kangwon National University)
Lamsal, Kabir (Department of Applied Plant Sciences, Kangwon National University)
Lee, Youn Su (Department of Applied Plant Sciences, Kangwon National University)
Publication Information
The Korean Journal of Mycology / v.41, no.2, 2013 , pp. 118-126 More about this Journal
Abstract
The experiment was carried out to analyze the inhibition effect of plant pathogenic fungi and growth promotion activity induced by the bacterial strains isolated from peatmoss. Among the isolated bacterial strains, B10-2, B10-4, B10-5 and B10-6 which showed more than 30% inhibition rate against Botrytis cinerea and Rhizoctonia solani in vitro, were further analyzed in the greenhouse for the growth promotion activity on lettuce (Lactuca sativa), pak-choi (Brassica compestris L. ssp. chinensis) and Chinese cabbage (Brassica campestris L. ssp. pekinensis). The results showed the treatment of B10-4 on lettuce showed the highest growth promotion activity with the leaf area ($169.17cm^2$), fresh weight (leaf: 40.29 g, root: 8.80 g)and dry weight (leaf: 11.24 g, root: 4.17 g), which was about two folds as compared to control. On pak-choi, the growth promotion rate was the highest with the leaf area of $112.87cm^2$, leaf fresh weight of 60.70 g, root fresh weight of 3.37 g, leaf dry weight of 14.34 g, and root dry weight of 1.90 g. As a result of treatment of B10-13 on chinese cabbage, the growth promotion rate was the highest with the leaf area ($293.56cm^2$), fresh weight (leaf: 113.67 g, root: 2.40 g) and dry weight (leaf: 6.03 g, root: 0.53 g). The production of Indole Acetic Acid (IAA) and Indole-3-Butylic Acid (IBA) were also analyzed in these bacterial isolates. The IAA and IBA analyses were carried out in all bacterial isolates each day within the 5 days of incubation period. The highest production of IAA was observed with $112.57{\mu}g/mg$ protein in B10-4 after 3 days of incubation and IBA production was the highest in B10-2 with $58.71{\mu}g/mg$ protein after 2 days of incubation. Also, phosphate solubilizing activity was expressed significantly in B10-13 in comparison to that of other bacterial isolates. Bacterial identification showed that B10-2 was Bacillaceae bacterium and B10-5 was Bacillus cereus, B10-4 and B10-6 were Bacillus sp. and B-13 was Staphylococcus sp. by ITS sequence.
Keywords
Bacillus sp.; Growth promotion; Indole Acetic Acid (IAA); Indole-3-Butylic Acid (IBA);
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Boix, C., Calvo, A. C., Imeson, A. C. and Soriano-Soto, M. D. 2001. Influence of soil properties on the aggregation of some Mediterranean soils and the use of aggregate size and stability as land degradation indicators. Catena 44:47-67.   DOI   ScienceOn
2 Dey, R., Pal, K. K., Bhatt, D. M. and Chauhan, S. M. 2004. Growth promotion and yield enhancement of peanut (Arachis phygaea L.) by application of plant growth-promoting rhizobacteria. Microbiol. Res. 159:371-394.   DOI   ScienceOn
3 Egamberdiyeva, D. 2007. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl. Soil Ecol. 36:184-189.   DOI   ScienceOn
4 Freitas, J. R., Banerjee, M. R. and Germida, J. J. 1997. Phosphate solubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola (Brassica napus L.). Biol. Fertil. Soils 24:358-364.   DOI   ScienceOn
5 Gray, E. J. and Smith, D. L. 2005. Intracellular and extracellular PGPR commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol. Biochem. 37:395-412.   DOI   ScienceOn
6 Handelsman, J., Raffel, S., Mester, E. H., Wunderlich, L. and Grau, C. R. 1990. Biological control of damping-off of alfalfa seeding with Bacillus cereus UW85. Appl. Environ. Microbiol. 56:713-718.
7 Kloepper, J. W. and Schroth, M. N. 1981. Relationship of in vitro antibiosis of plant growth-promoting rhizobacteria to plant growth and the displacement of root microflora. Phytopathol. 71:642-644.   DOI
8 Kloepper, J. W., Schroth, M. N. and Miller, T. D. 1980. Effects pf rhizospher colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathol. 70:1078-1082.   DOI
9 Lee, S. J. and Huh, K. Y. 2002. The effect of pyroligneous acid on turfgrass growth-The case of Yong-pyong golf course green. Korea Institute of Landscape Architecture. 30:95-104. (in Korean).   과학기술학회마을
10 Macros, A., Gagne, S. and Antoun, H. 1995. Effect of compost on rhizosphere microflora of tomato and on the incidence of plant growth-promotion rhizobacteria. Appl. Environ. Microbiol. 61:194-199.
11 Mayak, S., Tarosh, T. and Glick, B. R. 2004. Plant growthpromoting bacteria that confer resistance to water stress in tomatoes and peppers. Plant Sci. 166:525-530.   DOI   ScienceOn
12 Phae, C. G., Shoda, M. and Kita, N. 1992. Biological control of crown and root rot and bacterial wilt of tomato by Bacillus subtilis NB22. Ann. Phytopath. Soc. 58:329-339.   DOI
13 Topp, G. C., Reynolds, W. D., Cook, F. J., Kirby, J. M. and Carter, M. R. 1997. Physical attributes of soil quality. In; E. G. Gregorich and M. R. Carter (ed.) Soil Quality for Crop Production and Ecosystem Health. Develop. Soil Sci. 25:21-58.   DOI
14 Reynolds, W. D., Bowman, B. T., Drury, C. F., Tan, C. S. and Lu, X. 2002. Indicators of good soil physical quality : density and storage parameters. Geoderma 110:131-146.   DOI   ScienceOn
15 Rothrock, C. S. and Gottlied, D. 1981. Importance of antibiotic production in antagonism of selected Streptomyces species to two soil-borne plant pathogens. J. Antibiot. 34:830-835.   DOI
16 Turner, J. T. and Backman, P. A. 1991. Factors relating to peanut yield increases after seed treatment with Bacillus subtilis. Plant Dis. 75:347-353.   DOI
17 Wei, G., Kleopper, J. W. and Tuzun, S. 1996. Induced systemic resistance to cucumber diseases plant growth by plant promoting rhizobacteria under field conditions. Phytopathol. 86:221-224.   DOI
18 Whitelaw, M. A., Harden, T. J. and Helyar, K. R. 1999. Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Bio. Biochem. 31:655-665.   DOI   ScienceOn
19 Ahn, B. J., Cho, S. T., Cho, T. S., Lee, S. J. and Lee, Y. S. 2003. Effect of wood. charcoal and pyroligneous acid on soil microbiology and growth of red pepper. J. Kor. For. En. 22:49-56. (in Korean).   과학기술학회마을