The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Inhibition Effects Against Plant Pathogenic Fungi and Plant Growth Promotion by Beneficial Microorganisms

유용 미생물을 활용한 식물 병원 곰팡이의 억제와 식물 생장촉진 효과

  • Jung, Jin Hee (Department of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Sang Woo (Department of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Yun Seok (Department of Applied Plant Sciences, Kangwon National University) ;
  • Lamsal, Kabir (Department of Applied Plant Sciences, Kangwon National University) ;
  • Lee, Youn Su (Department of Applied Plant Sciences, Kangwon National University)
  • 정진희 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 김상우 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 김윤석 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 거비르 람살 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 이윤수 (강원대학교 농업생명과학대학 식물자원응용공학과)
  • Received : 2013.03.13
  • Accepted : 2013.06.20
  • Published : 2013.06.30
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Abstract

The experiment was carried out to analyze the inhibition effect of plant pathogenic fungi and growth promotion activity induced by the bacterial strains isolated from peatmoss. Among the isolated bacterial strains, B10-2, B10-4, B10-5 and B10-6 which showed more than 30% inhibition rate against Botrytis cinerea and Rhizoctonia solani in vitro, were further analyzed in the greenhouse for the growth promotion activity on lettuce (Lactuca sativa), pak-choi (Brassica compestris L. ssp. chinensis) and Chinese cabbage (Brassica campestris L. ssp. pekinensis). The results showed the treatment of B10-4 on lettuce showed the highest growth promotion activity with the leaf area ($169.17cm^2$), fresh weight (leaf: 40.29 g, root: 8.80 g)and dry weight (leaf: 11.24 g, root: 4.17 g), which was about two folds as compared to control. On pak-choi, the growth promotion rate was the highest with the leaf area of $112.87cm^2$, leaf fresh weight of 60.70 g, root fresh weight of 3.37 g, leaf dry weight of 14.34 g, and root dry weight of 1.90 g. As a result of treatment of B10-13 on chinese cabbage, the growth promotion rate was the highest with the leaf area ($293.56cm^2$), fresh weight (leaf: 113.67 g, root: 2.40 g) and dry weight (leaf: 6.03 g, root: 0.53 g). The production of Indole Acetic Acid (IAA) and Indole-3-Butylic Acid (IBA) were also analyzed in these bacterial isolates. The IAA and IBA analyses were carried out in all bacterial isolates each day within the 5 days of incubation period. The highest production of IAA was observed with $112.57{\mu}g/mg$ protein in B10-4 after 3 days of incubation and IBA production was the highest in B10-2 with $58.71{\mu}g/mg$ protein after 2 days of incubation. Also, phosphate solubilizing activity was expressed significantly in B10-13 in comparison to that of other bacterial isolates. Bacterial identification showed that B10-2 was Bacillaceae bacterium and B10-5 was Bacillus cereus, B10-4 and B10-6 were Bacillus sp. and B-13 was Staphylococcus sp. by ITS sequence.

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References

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