• Title/Summary/Keyword: B2C site

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The Thermal Dynamics of Fe Ion on the M-type Ba-ferrite (M형 Ba-Ferrite에서 Fe 이온의 열 진동에 관한 연구)

  • Sur, Jung-Chul;Ghim, Jin-Soo;Choi, Jong-Wan
    • Journal of the Korean Magnetics Society
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    • v.21 no.1
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    • pp.5-9
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    • 2011
  • M$\ddot{o}$ssbauer and Raman spectrum studies have been carried out on the 2b-site Fe ion in the Ba-ferrite (M-type). The thermal dynamics of Fe ion was analyzed by M$\ddot{o}$ssbauer spectra at different angles between the $\gamma$-ray direction and c-axis. The vibration on the 2b-site was more active compare to other direction and had very strong intensity in the Raman spectrum.

Identification of a Potential Tyrosine Phosphorylation Site on the NR2B Subunit of the N-methyl-D-aspartate Receptor (NMDA 수용체 아단위 2B의 Tyrosine 인산화 위치의 동정)

  • Il Soo Moon;Yong Wook Jung;Bok Hyun Ko
    • Journal of Life Science
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    • v.8 no.6
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    • pp.654-659
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    • 1998
  • The 2B subunit of N-methyl-D-aspartate (NMDA) receptors (NR2B) is the major phosphotyrosine-containing pro-tein in the postsynaptic density (PSD). In order to identify the site for tyrosine phosphorylation on NR2B, a mass spectrometry was applied on tryptic and endolys-C peptides. The NR2B subunit was isolated from N-octyl glucoside (NOG)-insoluble PSD fraction through SDS-PAGE and electroelution. The eluted protein was confirmed to be NR2B and phosphorylated on tyrosine by its cognate antibody and phosphotyrosine-specific antibody. By matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry of the peptides generated by digesting the eluted NR2B with trysin or endolys-C, a potential site for tyrosine phosphorylation could be identified as Tyr-1304.

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The Influence of B2C e-Learning Site Service Quality on Customer's Reuse Intention (B2C e-러닝 사이트의 서비스품질이 재이용의향에 미치는 영향)

  • Han, Dae-Mun;Kim, Yeong-Real;Kim, Jong-Woo
    • Journal of Korea Society of Industrial Information Systems
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    • v.11 no.5
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    • pp.245-260
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    • 2006
  • The e-learning market in korea has been growing rapidly over the past several years. We selects the variables well matched to service quality of e-learning site on the basis of independent variables of this study, sets the models as adding the factors newly noticed such as convenience of site use in e-learning. We also see the significance of the relationships between service quality of e-learning site, personal performance, customer satisfaction and customer's reuse intention.

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Occurrence of canine brucellosis in Korea and polymorphism of Brucella canis isolates by infrequent restriction site-PCR

  • Bae, Dong Hwa;Lee, Young Ju
    • Korean Journal of Veterinary Research
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    • v.49 no.2
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    • pp.105-111
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    • 2009
  • In this study, occurrence of canine brucellosis was surveyed in kennels, indoor dogs and stray dogs in Korea, and infrequent restriction site-polymerase chain reaction (IRS-PCR) was applied to analyze DNA polymorphism of Brucella canis (B. canis) isolates. Among a total of 501 dogs tested, B. canis antibodies by both rapid screening agglutination with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay were detected in only 14.1% of kennel dogs. There were no seropositive cases in indoor dogs and stray dogs. DNA polymorphism was observed in 16 B. canis isolates by the IRS-PCR. Sixteen isolates were tested with primers, PsalA, PsalC, PsalG and PsalT, and different primers produced different DNA patterns. In regard to the IRS-PCR pattern of 16 isolates, 9 (56.3%) belonged to the IRS-PCR type I. The remaining 7 were differentiated as type II, III and IV. An application of the primer PsalC provided discrimination between B. canis isolated in 2005 and others.

Effects of A-Site Sr and B-Site Substitution on the Dielectric Properties of BaTiO3 Ceramics (A-site Sr 및 B-site Ca 첨가 BaTiO$_3$ 세라믹스의 유전특성)

  • 박재관;오태성;김윤호
    • Journal of the Korean Ceramic Society
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    • v.28 no.9
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    • pp.689-695
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    • 1991
  • Dielectric properties of Ba1-$\chi$Sr$\chi$Ti1-yCayO3-y ceramics, where Sr and Ca were doped to Ba-site and Ti-site within the range of 0 x 0.24 and 0 y 0.05, respectively, were investigated. The substitution of Ca for Ti, which maintained the high resistivity of these formulations after sintering in a reducing atmosphere, was confirmed. Ca addition decreased the tetragonality c/a, increased the unit cell volume, and lowered Curie temperature, which were attributed to the occupancy of Ca2+ ions on Ti-sites. The lowering of Curie temperature by Ca addition was affected by the substitution of Sr for Ba-site; within 2 mol% of Ca, Curie temperature was lowered at a rate of 2$0^{\circ}C$ and 16$^{\circ}C$ per mol% of Ca at x=0 and x=0.08, respectively. Whereas the resistivity of the formulations without Ca was reduced to 107 {{{{ OMEGA }}cm, when sintered at low oxygen partial pressure of 10-9 MPa, the resistivity value higher than 1011 {{{{ OMEGA }}cm was maintained for the formulations containing Ca more than 0.5 mol%. Dielectric loss factor, tan$\delta$, was about 1% for most formulations.

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Dynamic Structure of Bacteriorhodopsin Revealed by $^{13}C$ Solid-state NMR

  • Saito, Hazime;Yamaguchi, Satoru;Tuzi, Satoru
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.110-113
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    • 2002
  • We demonstrate here a dynamic structure of bacteriorhodopsin (bR) as revealed by $^{13}$ C NMR studies on [3_$^{13}$ C]_,[1-$^{13}$ C]Ala- and/or Val-labeled wild type and a variety of site-directed mutants at ambient temperature. For this purpose, well-resolved (up to twelve) I$^{13}$ C NMR peaks were assigned with reference to the displacement of peaks due to the conformation-dependent I$^{13}$ C chemical shifts and reduced peak-intensities due to site-directed mutations. Revealed bR structure was not rigid as anticipated from 2D crystals of hexagonal array but a dynamically heterogeneous, undergoing a variety of local fluctuations depending upon specific site with frequency range of 10$^2$ -10$^{8}$ Hz. In particular, dynamics- dependent suppression of peaks turned out to be very sensitive to the motion of 10$^{-4}$ s and 10$^{-5}$ s interfered with frequency of magic angle spinning and proton decoupling, respectively. It is also noteworthy that such dynamic feature is strongly dependent upon the manner of 2D crystalline packing: $^{13}$ C NMR peaks of monomeric bR yielded either highly broadened or completely suppressed signals, depending upon the type of $^{13}$ C-labeled amino-acid residues.

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Study on the reducibility of substituted $LaMnO_3$ (치환된 $LaMnO_3$의 환원반응성에 대한 연구)

  • Lee, Sang-Beom;Jeon, Hyun-Pyo
    • The Journal of Natural Sciences
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    • v.14 no.2
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    • pp.41-54
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    • 2004
  • $LaMnO_3$ and A site substituted $La_{0.9}Sr_{0.1}MnO_3$(a=$5.33\AA$, c=$13.27\AA$), B site substituted $LaMn_{0.9}Cu_{0.1}O_3$(a=$5.52\AA$, c=$13.31\AA$) mixed oxides were prepared by Citrate sol-gel method. The powder X-ray diffraction patterns of these oxides were indexed with single phase hexagonal perovskite structures. According to the TRR result, oxygen stoichiometry of these oxides were oxidative nonstoichiometry as like $LaMnO_{3.16}$, $La_{0.9}Sr_{0.1}MnO_{3.10}$ and $LaMn_{0.9}Cu_{0.1}O_{3.14}$ Reduction reactions of un-substituted $LaMnO_3$ was two steps, but specific site(A site of B site) partially substituted $LaMnO_3$ oxides were procees to three reactions.

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Evaluation of Site-specific Seismic Response Characteristics at Town Fortress Areas Damaged by Historical Earthquakes (역사 지진 피해 발생 읍성 지역에 대한 부지 고유의 지진 응답 특성 평가)

  • Sun, Chang-Guk;Chung, Choong-Ki;Kim, Dong-Soo;Kim, Jae-Kwan
    • The Journal of Engineering Geology
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    • v.17 no.1 s.50
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    • pp.1-13
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    • 2007
  • In order to evaluate the local site effects at two town fortress areas in Korea where stone parapets were col-lapsed by historical earthquakes, site characteristics were assessed using site investigations such as borehole drillings and seismic tests. Equivalent-linear site response analyses were conducted based on the shear ways velocity ($V_s$) profiles and geotechnical characteristics determined from site investigations. The study sites are categorized as site classes C and B according to the mean $V_s$ to 30 m ranging from 500 to 850 m/s, and their site periods are distributed in the short period range of 0.06 to 0.16 sec, which contains the natural period of fortress wall and stone parapet. From the results of site response analyses in the study areas, for site class C indicating most of site conditions, contrary to site class B, the short-period (0.1-0.5 sec) and mid-period (0.4-2.0 sec) site coefficients, $F_a$ and $F_v$ specified in the Korean seismic design guide, underestimate the ground motion in short-period band and overestimate the ground motion in mid-period band, respectively, due to the high amplification in short period range, which represent the site-specific seismic response characteristics. These site-specific response characteristics indicate the potential of resonance in fortress walls during earthquake and furthermore could strongly affect the collapse of parapets resulted from seismic events in historical records.

e-CRM을 위한 B2C Web Site 고객행동모델 분석 및 평가방법에 관한 연구

  • 이경록;서장훈;박명규
    • Proceedings of the Safety Management and Science Conference
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    • 2002.11a
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    • pp.239-246
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    • 2002
  • In this report, we provide the focus on suggesting a method of estimating & measurement of CBM(Customer Behavior Model). Through the use of internet, a new trend of business for e-CRM on B2C Web Site which is now known as EC has emerged. The Purpose of this study is to identify the relationship between the customers of a shopping mall and CBM characteristics. Result showed that there is a significant relationship between the some customers pattern of shopping mall and CBM, CVM(Customer Visit Model).

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Construction of Transformation Method for Streptomyces scabiei ATCC 49173 Producing Phytotoxin (식물독소를 생산하는 Streptomyces scabiei ATCC 49173의 형질전환법 구축)

  • Jang, Bo-Youn;Ha, Heon-Su;Choi, Sun-Uk
    • KSBB Journal
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    • v.25 no.2
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    • pp.167-172
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    • 2010
  • Streptomyces scabiei producing phytotoxin called thaxtomin, which cause scab disease on economically important crops such as potato. For molecular genetics study of S. scabiei an effective transformation method was established based on conjugal transfer from Escherichia coli ET12567 (pUZ8002) using a phiC31-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 50 mM $MgCl_2$. In addition, the sequence and location of the chromosomal integration attB site of S. scabiei was identified for the first time in the strains producing thaxtomin by the southern blot analysis of exconjugants and the sequencing of plasmid containing DNA flanking the insertion sites from exconjugant chromosome. Similar to the case of Streptomyces species, a single phiC31 attB site of S. scabiei is present within an ORF encoding a pirin-homolog.