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Formulation of Bacillus amyloliquefaciens A-2 and Its Efficacy to Control Tomato Leaf Mold Caused by Fulvia fulva (길항세균 Bacillus amyloliquefaciens A-2를 이용한 토마토 잎곰팡이병 방제용 미생물 제제)

  • Kong, Hyun-Gi;Chun, Ock-Joo;Choi, Ki-Hyuck;Lee, Kwang-Youll;Baek, Joung-Woo;Kim, Hyun-Ju;Murugaiyan, Senthilkumar;Moon, Byung-Ju;Lee, Seon-Woo
    • Research in Plant Disease
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    • v.16 no.1
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    • pp.27-34
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    • 2010
  • This study was performed to develop a formulation using an antagonistic bacterium Bacillus amyloliquefaciens A-2 to control tomato leaf mold caused by Fulvia fulva. B. amyloliquefaciens A-2 was grown in a medium with rice oil and mixed with various carrier and additives. One of the formulations, A2-MP, showed the best disease control value among the tested formulations. The disease control value of A2-MP at 100-fold and 500-fold diluted treatment was not significantly different from that of chemical fungicide triflumizole in a growth chamber. Although disease control effect was decreased by serial diluted treatment of the prepared A2-MP, 1,000-fold diluted treatment of A2-MP still showed high disease control value of 72.0%. For the green house experiments, the disease control values of A2-MP was indicated as 79.4% which is similar to that of chemical fungicide, triflumizole showing 79.6%. When the disease control activity of the formulation A2-MP was compared in tomato production conditions, disease control values of 100-fold diluted A2-MP and 3,000 fold diluted triflumizole exhibited 60%, 81.6%, respectively. The disease control efficiency by A-2MP was 73% of the disease control value of chemical fungicide. The formulation A-2MP maintained the stable bacterial viability and disease control activity when stored at $4^{\circ}C$. This result suggested that A-2MP develped from B. amyloliquefaciens A-2 could be used to control tomato leaf mold.

Survey on Menstruation of Middle & High School Girls in an Urban Area (일부 도시지역에 있어서 중, 고교생의 월경에 관한 조사연구)

  • 김명엽;강현숙
    • Journal of Korean Academy of Nursing
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    • v.2 no.1
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    • pp.63-72
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    • 1971
  • The conclusions were obtained from the detailed survey of 1,285 students experiencing menstruation, who were chosen among 1,717 students in middle & high school girls in Seoul. The survey was conducted during July 20-July 24, 1971. 1. Age of Menarche An average age of menarche was 13.3$\pm$1.07. The earliest age of menarche was 9 and the latest age 18. Ages of menarche were between 12 and 14 in 84.3 percent of the students surveyed. a. By present age distribution, the aged students were lower, than younger students in the average age of menarche. b. By father′s educational levels, among the students whose fathers were graduated from high schools, college & ever the earliest average age of menarche was found with 13.2, and among the students whose fathers were graduated from primary schools that wag latest with 13.6. c. By father′s occupations, among the students whose fathom engaged in "workers not classifiable"the earliest age of menarche was found with 12.5$\pm$0.27, among the students whose fathers were in "service business"the second was 12.9$\pm$1.07, and among the students whose fathers were in "miners, quarrymen and related workers"that was latest with 13.8$\pm$1.14. d. By economic status, among the students of "wealthy"families the age of menarche was 13.1$\pm$0.25, the among the students of "ordinary"families the lags of menarche 13.3$\pm$1.06. and the among the students of "poor" families that was 13.8$\pm$0.31. e. By home discipline, among the students being treated "rigid" the age of menarche was 13.5$\pm$1.13, among the students being treated "moderate"the age of menarche was 13.3$\pm$0.22, and those being treated "indifferent" that was 13.0$\pm$0.26. f. By students physical condition, among the students of "good" condition the average of menarche was 13.3$\pm$0.16, and among the students "poor" that was 13.5$\pm$0.31. 2. Menstruation a. For the six months after the average of menarche 39.0 percent of the students had normal menstruations, and 61.3 percent of them had abnormal ones. Of the students with abnormal menstruation 21.7 percent had abnormal menstruation from time to time, 25.4 percent had no menstruation for one month to three months, 7.2 percent had menstruation for four to six months and 6.7 per cent had no menstruation for more than sin months. Most students became to have normal menstruations a few months later the age of menarche. b. At the time interviewed, the percentile of cycle of menstruation as following: 23 days types: 46.8 percent 30 days types: 40.6 percent others : 12.6 percent The average cycle of menstruation was every 28.9 days. c. The average duration of menstruation is 4.69 days. d. The subjective symptoms during menstruation period: Out of the total 89.7 per cent had some pains, while 10.3 percent had no symptom. Among the symptoms, abdominal pain occupied 29.9 percent, neurotic symptoms 19.0 percent and lumbago 15.1 percent. e. By attitude or Action at first physical change, "Treated it by own experience" : 30.0 percent "Don′t know what to do because of ignorance" : 20.1 percent "Asked others about it" : 43.0 percent

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Effects of Xylitol and Grapefruit Seed Extract on Sensory Value and Fermentation of Baechu Kimchi (자일리톨과 자몽씨추출물이 배추김치의 관능성과 발효숙성에 미치는 영향)

  • Moon, Sung-Won;Shin, Hyun-Kyung;Gi, Geun-Eog
    • Korean Journal of Food Science and Technology
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    • v.35 no.2
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    • pp.246-253
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    • 2003
  • Application of xylitol (Xyl) and grapefruit seed extract (GSE) to improve the quality and preservation of baechu kimchi was attempted. Xylitol and grapefruit seed extract at various combinatory concentrations were added into baechu kimchi and fermented for 25 days at $10^{\circ}C$. Assay was performed on sensory value, acidity, and bacterial growth. Addition of 0.1% GSE and 2% Xyl showed the highest score in the overall acceptability, sour taste, and texture. Score of intensity characteristics in smell and sour taste were the highest in the control and that of texture the highest in 0.1% GSE plus 2% Xyl treatment. The pH decreased, and titratable acidity, and growth of total viable cells and lactic acid bacteria were remarkably retarded in 0.1% GSE plus 2% Xyl group compared to the control. Results showed that application of 2% Xyl plus 0.1% GSE to the kimchi fermentation enhanced sensory value of the fermented product and extended the storage period by about twofold.

Numerical simulation of hollow steel profiles for lightweight concrete sandwich panels

  • Brunesi, E.;Nascimbene, R.;Deyanova, M.;Pagani, C.;Zambelli, S.
    • Computers and Concrete
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    • v.15 no.6
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    • pp.951-972
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    • 2015
  • The focus of the present study is to investigate both local and global behaviour of a precast concrete sandwich panel. The selected prototype consists of two reinforced concrete layers coupled by a system of cold-drawn steel profiles and one intermediate layer of insulating material. High-definition nonlinear finite element (FE) models, based on 3D brick and 2D interface elements, are used to assess the capacity of this technology under shear, tension and compression. Geometrical nonlinearities are accounted via large displacement-large strain formulation, whilst material nonlinearities are included, in the series of simulations, by means of Von Mises yielding criterion for steel elements and a classical total strain crack model for concrete; a bond-slip constitutive law is additionally adopted to reproduce steel profile-concrete layer interaction. First, constitutive models are calibrated on the basis of preliminary pull and pull-out tests for steel and concrete, respectively. Geometrically and materially nonlinear FE simulations are performed, in compliance with experimental tests, to validate the proposed modeling approach and characterize shear, compressive and tensile response of this system, in terms of global capacity curves and local stress/strain distributions. Based on these experimental and numerical data, the structural performance is then quantified under various loading conditions, aimed to reproduce the behaviour of this solution during production, transport, construction and service conditions.

Regeneration of Immune System after Syngeneic Bone Marrow Transplantation in Irradiated Mice: I. The Changes of Cell Number in Peripheral blood and Lymphatic Organs and Composition of Lymphocyte Subsets (마우스 동계골수이식 후 면역체계의 재생: I. 말초혈액 및 림프장기 세포의 숫적 변 화와 림프구아군의 분포)

  • Kim, Sung-Ho;Oh, Heon;Lee, Song-Eun;Kim, Soon-Tae;Jo, Sung-Kee;Hyun, Byung-Hwa;Ryu, Si-Yun;Daynes, Raymond A.
    • Korean Journal of Veterinary Pathology
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    • v.1 no.1
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    • pp.1-6
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    • 1997
  • The cellularity and composition of the spleen lymph node thymus and peripheral blood and tempo of regeneration were studied at various time points after syngeneic bone marrow transplantation(BMT) in C3H/Hen mice. Significant depression of absolute lymphocyte count was noted on week 1 after lethal whole-body irradiation and BMT. In comparison to the lymph node thymus and spleen had an rapid regeneration of cellularity. The distinct cell populations($CD4^+,\;CD8^+,\;CD28^+,\;B220^+) have determined in the lymphoid tissue of mice subjected to irradiation. The relative representation of these subpopulations was significantly different from that in nonirradiated control. $CD4^+\;and\;CD8^+$ cells were present in very low numbers whereas the $B220^+$ cells reached more than normal range at 2 weeks after BMT. The number of $CD4^+$ cells returned to normal relatively soon than $CD8^+$ cell. At week 4 after BMT, the cellularity and composition of spleen lymph node and peripheral blood lymphocyte reached about 50% of the normal range therefore we can choose this time point for the other tests of immune function after BMT.

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Effect of extract temperature and duration on antioxidant activity and sensory characteristics of Ulmus pumila bark extract (추출온도 및 시간에 따른 유백피 추출물의 항산화 활성과 음료의 관능적 특성)

  • Cho, Myoung Lae;Oh, Yu-Na;Ma, Jin-Gyeong;Lee, Su-Jin;Choi, Young-Hee;Son, Dong-Hwa;Jang, Eun Hee;Kim, Jong-Yea
    • Food Science and Preservation
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    • v.23 no.7
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    • pp.995-1003
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    • 2016
  • Ulmus pumila L. bark underwent distilled water extraction under three temperature condition ($4^{\circ}C$, room temperature, or $80^{\circ}C$) and two extraction times (1, or 5 min) in order to develop a functional beverage products. Changes in yield, pH, color, total phenolic (TP) content, tannin content and antioxidant activity of the aqueous extracts were evaluated for each extraction temperature and duration. Extraction conditions did not affect yield or pH value of the extracts; however CIE $b^*$ values were high in extracts prepared under high extraction temperature ($80^{\circ}C$) and long extraction duration (5 min) conditions. Both extraction temperature and duration affected the TP and tannin contents of the extracts; however, all extraction conditions resulted in ${\geq}450\;mg\;GAE/g$ TP content and ${\geq}80\;mg\;CE/g$ tannin content. All extracts exhibited ABTS and DPPH radical scavenging ability similar to that of vitamin C. Nitric oxide inhibition activity was lower in the 5 min duration sample than in the 1 min sample. The $4^{\circ}C$ extraction temperature produced an extract with the highest reducing power and hydrogen peroxide values. Extraction temperature also affected sensory evaluation results with the $80^{\circ}C$ extraction temperature producing significantly higher flavor, bitterness, and color score, than those obtained under $4^{\circ}C$ and room temperature extraction conditions.

Changes in Quality Characteristics of Venison Jerky Manufactured under Different Dry Time during Storage (건조시간을 달리하여 제조한 사슴고기 육포의 저장 중 품질 특성 변화)

  • Kim Il-Suk;Jin Sang-Keun;Park Ki-Hoon;Kim Dong-Hoon;Hah Kyung-Hee;Park Seok-Tae;Kwuak Kyung-Rak;Park Jung-Kwon;Kang Yang-Su
    • Food Science of Animal Resources
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    • v.26 no.2
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    • pp.166-174
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    • 2006
  • To determine the proper processing and storage conditions, physico-chemical, microbial and sensory properties of venison jerky under different dry times were measured during storage at $30^{\circ}C$ for 28 days. Samples were dried for 3 hr (T1), 4 hr (T2) and 5 hr (T3) at $75^{\circ}C$ in the smoke chamber, respectively. The pH of T1 was slightly lower than those of T2 and T3 as storage time increased. As dry and storage time increased, TBARS of T2 and T3 were significantly higher (p<0.05) than that of T1. In meat color, $L^*$ values of T3 showed slightly higher than those of T1 and T2, while at values were not clearly tendency by the passage of storage time. $b^*$ values of T2 and T3 were higher than that of T1. The water activity were significantly lower (p<0.05) in ,the order of T3$log_{10}$ CFU/g until 28 days and its number were accepted by sensory evaluation. In conclusions, T2 and T3 showed slightly high overall acceptability and lipid oxidative stability compared to T1 conditions. These results indicated that longer dry time ($4{\sim}5 hr$) of venison jerky would be better characteristics as compared to shorter dry time (3 hr) with increased storage time at $30^{\circ}C$.

Evaluation of The Highway Design Speed Determination Process Using Case Studies (Reclassifying Functions and Terrain Types) (사례분석을 통한 도로설계속도 결정방법론 적용성 평가 (기능 재분류와 지형특성 이용))

  • Sim, Gwan-Bo;Choe, Jae-Seong
    • Journal of Korean Society of Transportation
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    • v.24 no.2 s.88
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    • pp.101-112
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    • 2006
  • Currently, highway design speed is determined by considering highway function, area type, and terrain type. Sometime it is pointed out that determining a reasonable design speed which is both efficient and safe is not an easy task and that Practicing engineers often select an unsuitable design speed on purpose, capitalizing on some ambiguous and discretionary expressions in describing the highway design speed. This undesirable Problem is arising mostly due to the fact, that the current geometric design standard fails to include rolling terrain type and can not reflect the whole characteristics of land use patterns adjacent to the design highway route. A recent research was Published considering this problem and it attempted to improve the highway design speed determining process. In this research Project, tn see the effects of this recently developed procedure, a new and reduced design speed was calculated based on the new Procedure and subsequently another highway design route was selected. The travel time. construction cost. and the expected degree of safety associated with the new route were assessed to be compared with the ones with the existing procedure. As a result. it was found that the new procedure was successful in reflecting the localities such as terrain type and area type into better determining highway design speed, eliminating much of highway engineers' discretion when applying engineering judgments. Also the new Procedure is keen to produce a more economical highway project. In other words, despite of producing reduced amount of user benefits accrued, in the new highway route, the construction cost has been cut significantly leading to higher values in B/C. NPV, and IRR. Also EMME-II output, which Provided the link assigned volumes, rendered only a slightly reduced Levels of Service along surrounding links in the study network. This reduction was believed to occur because of lower design speed and it had been expected from the beginning.

Treatment of Reproductive Dysfunctions and Reproductive Monitoring Using Ultrasonography in Dairy Cow (초음파 검사에 의한 젖소 번식 검진과 번식 장애 치료)

  • Lim, W.H.;Oh, K.S.;Seo, G.J.;Hwang, S.S.;Kim, B.S.;Bae, C.S.;Kim, S.H.;Kim, J.T.;Park, I.C.;Park, S.G.;Son, C.H.
    • Journal of Embryo Transfer
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    • v.21 no.3
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    • pp.217-223
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    • 2006
  • This study was carried out principally to obtain the basic data for the improvement of the reproductive performance and production using plasma progesterone assay and ultrasonography in dairy cow. The results obtained from this studies were as follows. The results of reproductive examination in 85,983 cows were ovarian diseases 40,399 (47.0%), uterine diseases 11,912 (13.9%), pregnancy or pregnant failures 26,587 (30.9%), adhesion of reproductive tracts 172 (0.2%), freemartin 8 (0.01%), and others 6,905 (8.3%), respectively. The treatment status of reproductive dysfunction in 30,241 cows were silent heat or error of estrus detection 14,909 (49.3%), follicular cysts 3,750 (12.4%), luteal cysts 907 (3.0%), inactive ovaries 665 (2.2%), granulosa cell tumor of ovary 3 (0.01%) and endometritis 6,986 (23.1%), respectively. The indices of reproductive efficiency after the periodical examination of reproductive status were as follows; the mean intercalving inteual was reduced from 475 days at the first examination to 381 days at the last examination of reproductive status, the mean interval calving to conception was reduced from 186 to 98 days, the mean interval calving to first service was reduced from 106 to 66 days, the cows showing heat by 60 days postpartum were increased from 32 to 90%, the mean conception rate to first service was increased from 42 to 64%, and the mean service per conception was reduced from 2.6 to 1.8 times, respectively.

A Standard Rose Cultivar, 'To Dios', with Numerous Peach-colored Petals (꽃잎 수가 많은 복숭아색 스탠다드 절화 장미 '투디오스' 육성)

  • Heo, Moon-Sun;Hwang, Soo-Kyung;Yoon, Jae-Soo;Kang, Byoung-Cheorl
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.799-806
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    • 2016
  • A standard rose cultivar, 'To Dios'(Rosa hybrida) was selected for use as a cut flower from the progeny of a cross between 'Vanilla perfume' and 'Marcia' at the research and development division of the Goyang-si Agricultural Technology Center in 2013. 'Vanilla perfume', an orange-colored (RHS Orange Group 27C) standard rose cultivar with 48 petals, was used as the female plant. 'Marcia', a white-colored (RHS Green white Group 157B) standard rose cultivar with 96 petals was used as the male parent. A cross was made in 2009 and seedlings were produced. Selections were made between 2010 and 2013, and a plant with good cut flower traits was finally selected and named 'To Dios'. 'To Dios' is a standard rose with large flowers of 11.2 cm in diameter and 128 peach-colored (RHS Red Group 36B) petals per flower. Vase life of this cultivar is up to 15 days. It takes 47 days from pruning to blooming and cut flower productivity is approximately $160stems/m^2$ per year. 'To Dios' was registered as a new cultivar No. 4875 with the Korea Seed & Variety Service on March 19, 2014.