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http://dx.doi.org/10.5423/RPD.2010.16.1.027

Formulation of Bacillus amyloliquefaciens A-2 and Its Efficacy to Control Tomato Leaf Mold Caused by Fulvia fulva  

Kong, Hyun-Gi (Dept. of Applied Biology, Dong-A University)
Chun, Ock-Joo (Dept. of Applied Biology, Dong-A University)
Choi, Ki-Hyuck (Dept. of Applied Biology, Dong-A University)
Lee, Kwang-Youll (Dept. of Applied Biology, Dong-A University)
Baek, Joung-Woo (Dept. of Applied Biology, Dong-A University)
Kim, Hyun-Ju (National Plant Quarantine Service)
Murugaiyan, Senthilkumar (Dept. of Applied Biology, Dong-A University)
Moon, Byung-Ju (Dept. of Applied Biology, Dong-A University)
Lee, Seon-Woo (Dept. of Applied Biology, Dong-A University)
Publication Information
Research in Plant Disease / v.16, no.1, 2010 , pp. 27-34 More about this Journal
Abstract
This study was performed to develop a formulation using an antagonistic bacterium Bacillus amyloliquefaciens A-2 to control tomato leaf mold caused by Fulvia fulva. B. amyloliquefaciens A-2 was grown in a medium with rice oil and mixed with various carrier and additives. One of the formulations, A2-MP, showed the best disease control value among the tested formulations. The disease control value of A2-MP at 100-fold and 500-fold diluted treatment was not significantly different from that of chemical fungicide triflumizole in a growth chamber. Although disease control effect was decreased by serial diluted treatment of the prepared A2-MP, 1,000-fold diluted treatment of A2-MP still showed high disease control value of 72.0%. For the green house experiments, the disease control values of A2-MP was indicated as 79.4% which is similar to that of chemical fungicide, triflumizole showing 79.6%. When the disease control activity of the formulation A2-MP was compared in tomato production conditions, disease control values of 100-fold diluted A2-MP and 3,000 fold diluted triflumizole exhibited 60%, 81.6%, respectively. The disease control efficiency by A-2MP was 73% of the disease control value of chemical fungicide. The formulation A-2MP maintained the stable bacterial viability and disease control activity when stored at $4^{\circ}C$. This result suggested that A-2MP develped from B. amyloliquefaciens A-2 could be used to control tomato leaf mold.
Keywords
Bacillus amyloliquefaciens; Biocontrol formulation; Fulvia fulva; Tomato leaf mold;
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Times Cited By KSCI : 1  (Citation Analysis)
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