• Title/Summary/Keyword: B16F0

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Genetic Polymorphisms of Blood Proteins and Enzymes in Korean Native Cattle (재래한우의 혈액단백질 및 효소의 유전적 다형현상)

  • Sang, Byung Chan;Ryoo, Seung Heui;Lee, Sang Hon;Han, Sung Wook;Lee, Chang Soo
    • Korean Journal of Agricultural Science
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    • v.22 no.2
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    • pp.170-179
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    • 1995
  • This study was conducted to investigate the genetic constitution of blood proteins and enzymes in 238 Korean Native cattle reared at Korean Native Cattle Breeding Center, National Livestock Cooperative Federation. The genetic polymorphisms of post-transferrin-2(pTf-2), transferrin(Tf), post-albumin(pAlb), albumin(Alb), ceruloplamin(Cp), amylase-I(Am-I) and hemoglobin(Hb) were analyzed by using PAGE(polyacrylamide gel electrophoresis) and STAGE(starch gel electrophoresis). The genotypes and gene frequencies were estimated at these loci for each blood proteins and enzymes. The results obtained from this study were summarized as follows : 1. The pTf-2 locus were identified to be genetically controlled by codominant alleles designated pTf-2 F and S, and the distribution of genotypes were 46.22, 46.64 and 7.14% for pTf-2 FF, FS and SS types, and the gene frequencies of the pTf-2 F and S allele were 0,695 and 0.305, respectiveley. 2. The Tf locus were found to be controlled by Tf A, D1, D2 and E alleles, and the distributioin of genotypes were 0.84, 13.87, 13.03, 10.92, 22.27, 12.61, 2.94, 15.51, 6.72 and 1.68% for Tf AA, AD1, AD2, AE, D1D1, D1D2, D1E, D2E and EE types, and the gene frequencies of Tf A, D1, D2 and E were 0.197, 0.430, 0.191 and 0.081, respectively. 3. The pAlb locus were observed to be controlled by two alleles, pAlb F and S, and the distribution of genotypes were 42.86, 33.19 and 23.95% for pAlb FF, FS and SS types, and the gene frequencies were 0.595 and 0.405 for Tf F and S allele, respectively. Also the gene frequencies of Alb was 1.000 of Alb A allele. 4. The Cp locus were identified to be controlled by Cp F and S allele, and the distribution of genotypes were 23.11, 34.87 and 42.02% for Cp FF, FS and SS types, and the gene frequencies were 0.405 and 0.595 for Cp F and S allele, respectively. 5. The Am-I locus were observed to be genetically controlled by Am-I B and C allele, and the distribution of genotypes were 51.26, 16.81 and 31.92% for Am-I BB, BC and CC types, and the gene frequencies of Am-I B and C alleles were 0.597 and 0.403, respectively. 6. The Hb locus were found to be controlled by Hb A and B alleles, and the distribution of genotypes were 93.19, 16.39 and 0.42% for Hb AA, AB and BB types, and the gene frequencies of Hb A and B alleles were 0.914 and 0.086, respectively.

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Antitumor Activity of the Medicinal Formula Kilkyungtang and Two Modified Kilkyungtangs in Vivo. I (생약복합제제 길경탕 및 가미길경탕의 항암효과 (제 1 보))

  • Kim, Sung-Hoon;Park, Kyung-Sik;Ryu, Shi-Yong
    • Korean Journal of Pharmacognosy
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    • v.27 no.1
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    • pp.37-41
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    • 1996
  • The prescription, Kilkyungtang (KKT), which originally consists of twelve kinds of medicinal plant materials and was used as a decoction for the treatment of malignant tumors and two modified Kilkyungtangs (KKT-1 and KKT-2), supplemented by the additional crude drug to KKT (KKT-1:Houttuyniae herba, and KKT-2:Oldenlandiae diffusae herba) were investigated on their antitumoral properties, in vivo respectively. All KKTs were found to exhibit significant life time-prolonging effects when they were administered orally to Sarcoma-180 bearing ICR mice for 7 days. (ILS was estimated as 20% in KKT, 42% in KKT-1 and 57% in KKT-2). A profound lessening of tumor weights was also observed when KKTs were administered to $B16-F_0$ bearing C57B/6 mice.

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$M\"{o}ssbauer$ studies of $NdFe_{10.7}TiM{0.3}(M\;=\;B,\;Ti)$ ($NdFe_{10.7}TiM{0.3}(M\;=\;B,\;Ti)$$M\"{o}ssbauer$ 연구)

  • 김철성;이용종;김윤배;김창석
    • Journal of the Korean Magnetics Society
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    • v.5 no.1
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    • pp.64-70
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    • 1995
  • The authors have studied crystallographic and magrletic properties of $NdFe_{10.7}TiM_{0.3}(M=B,\;Ti)$ by X-ray diffraction, VSM magnetometer, and Mossbauer spectrometer. The Alloys were prepared by arc-melting under argon atmosphere. The $NdFe_{10.7}TiM_{0.3}$ has pure single phase, whereas the $NdFe_{10.7}Ti_{1.3}$ contains some $\alpha-Fe$, from powder X-ray diffractometry. The $NdFe_{10.7}TiM_{0.3}$ has the $ThMn_{12}$-type tetragonal structure with $a_{0}=8.587\;{\AA}\;and\;c_{0}=4.788\;{\AA}$. The Curie temperature ($T_c$) is $570{\pm}3\;K$ from $M\"{o}ssbauer$ spectroscopy performed at various temperatures ranging from 13 to 770 K. Each spectrum of below $T_c$ was fitted with five subspectra of Fe sites in the structure ($8i_{1},\;8i_{2},\;8j_{1},\;8j_{2}\;and\;8f$). The area fraction of the subspectra at room temperature are 16.4, 8.2, 14.8, 21.3 and 39.3 %, respectively. Magenetic hyperfine fields for the Fe sites decrease in the order, $H_{hf}(8i)>H_{hf}(8j)>H_{hf}(8f)$.

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NONLINEAR DIFFERENTIAL EQUATIONS OF SECOND ORDER IN A HILBERT SPACE

  • Kim, RakJoong
    • Korean Journal of Mathematics
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    • v.16 no.1
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    • pp.91-101
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    • 2008
  • Let H be a Hilbert space. Assume that $0{\leq}{\alpha}$, ${\beta}{\leq}1$ and r(t) > 0 in I = [0, T]. By means of the fixed point theorem of Leray-Schauder type the existence principles of solutions for two point boundary value problems of the form $\array{(r(t)x^{\prime}(t))^{\prime}+f(t,x(t),r(t)x^{\prime}(t))=0,\;t{\in}I\\x(0)=x(T)=0}$ are established where f satisfies for positive constants a, b and c ${\mid}{f(t,x,y){\mid}{\leq}a{\mid}x{\mid}^{\alpha}+b{\mid}y{\mid}^{\beta}+c\;\;for\;all(t,x,y){\in}I{\times}H{\times}H$.

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Variance Components and Genetic Parameters for Milk Production and Lactation Pattern in an Ethiopian Multibreed Dairy Cattle Population

  • Gebreyohannes, Gebregziabher;Koonawootrittriron, Skorn;Elzo, Mauricio A.;Suwanasopee, Thanathip
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.9
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    • pp.1237-1246
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    • 2013
  • The objective of this study was to estimate variance components and genetic parameters for lactation milk yield (LY), lactation length (LL), average milk yield per day (YD), initial milk yield (IY), peak milk yield (PY), days to peak (DP) and parameters (ln(a) and c) of the modified incomplete gamma function (MIG) in an Ethiopian multibreed dairy cattle population. The dataset was composed of 5,507 lactation records collected from 1,639 cows in three locations (Bako, Debre Zeit and Holetta) in Ethiopia from 1977 to 2010. Parameters for MIG were obtained from regression analysis of monthly test-day milk data on days in milk. The cows were purebred (Bos indicus) Boran (B) and Horro (H) and their crosses with different fractions of Friesian (F), Jersey (J) and Simmental (S). There were 23 breed groups (B, H, and their crossbreds with F, J, and S) in the population. Fixed and mixed models were used to analyse the data. The fixed model considered herd-year-season, parity and breed group as fixed effects, and residual as random. The single and two-traits mixed animal repeatability models, considered the fixed effects of herd-year-season and parity subclasses, breed as a function of cow H, F, J, and S breed fractions and general heterosis as a function of heterozygosity, and the random additive animal, permanent environment, and residual effects. For the analysis of LY, LL was added as a fixed covariate to all models. Variance components and genetic parameters were estimated using average information restricted maximum likelihood procedures. The results indicated that all traits were affected (p<0.001) by the considered fixed effects. High grade $B{\times}F$ cows (3/16B 13/16F) had the highest least squares means (LSM) for LY ($2,490{\pm}178.9kg$), IY ($10.5{\pm}0.8kg$), PY ($12.7{\pm}0.9kg$), YD ($7.6{\pm}0.55kg$) and LL ($361.4{\pm}31.2d$), while B cows had the lowest LSM values for these traits. The LSM of LY, IY, YD, and PY tended to increase from the first to the fifth parity. Single-trait analyses yielded low heritability ($0.03{\pm}0.03$ and $0.08{\pm}0.02$) and repeatability ($0.14{\pm}0.01$ to $0.24{\pm}0.02$) estimates for LL, DP and parameter c. Medium heritability ($0.21{\pm}0.03$ to $0.33{\pm}0.04$) and repeatability ($0.27{\pm}0.02$ to $0.53{\pm}0.01$) estimates were obtained for LY, IY, PY, YD and ln(a). Genetic correlations between LY, IY, PY, YD, ln(a), and LL ranged from 0.59 to 0.99. Spearman's rank correlations between sire estimated breeding values for LY, LL, IY, PY, YD, ln(a) and c were positive (0.67 to 0.99, p<0.001). These results suggested that selection for IY, PY, YD, or LY would genetically improve lactation milk yield in this Ethiopian dairy cattle population.

RESULTS FROM ADAPTABILITY TRIAL OF RAMBOUILLET SHEEP AND THEIR CROSSBREEDING WITH KAGHANIS. EFFECTS ON EWE MATING WEIGHT, WOOL PRODUCTION, LITTER SIZE AND LAMB GROWTH

  • Nawaz, M.;Meyer, H.H.;Jadoon, J.K.;Naqvi, M.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.3
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    • pp.481-485
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    • 1992
  • In order to upgrade native sheep, Rambouillet (R) rams were mated to Kaghani (K) ewes to generate F1 ($R{\times}K$) crossbred ewes. Crossbred ewes were backcrossed to Rambouillet rams to produce B1 ($R{\times}F1$), B2 ($R{\times}B1$) and B3 ($R{\times}B2$) genotypes. Weaning weight of 2605 lambs and wool weight of 2378 mature ewe records, representing R, K, F1, B1, B2 and B3 genotypes, were analyzed to compare genetic variation among genotypes produced during upgrading process and identify genotypes of the highest performance. Performance of Rambouillets was also evaluated under semi-temperate climate. Data were adjusted for yearly variation considering Rambouillet as a control. Genotypes influenced lambs weaning weight (p<.01). B1 lambs were heaviest (18.4 kg) followed in order by B2, F1, B3, R and K lambs (18.3, 17.9, 16.9, 16.8 and 13.2 kg, respectively). The highest wool production was 2.5 kg from R ewes followed by B2 (2.3), B3 (2.3), F1 (2.0) and K (1.2) ewes (p < .01). Ewe mating weight, reproduction, growth and wool production of Rambouillets deteriorated significantly after the first decade of their importation. Compared with the first phase (1959-1971), ewe mating weight, litter size, birth weight, lamb weaning weight and wool production declined by 20, 23, 32 and 36%, respectively, in the second phase (1972-1988).

쌍끌이 중층트롤어법의 연구 ( 2 ) - 모형어구의 깊이에 관하여 - ( A Study on the Pair Midwater Trawling ( 2 ) - Working Depth of the Model Net - )

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    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.31 no.1
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    • pp.45-53
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    • 1995
  • Working depth of the model net was determined by using of the same experimental tank and the same model net that used in the forwarded report in a series studies. The depth of the net which indicates the depth of the head rope from the water surface, was determined by the photographs taken in front of the net mouth with the combination of towing velocity, warp length and distance between paired boats. The results obtained can be summarized as follows: 1. Working depth of model nets A and B was varied in the range of 0.09~1.66$m$,and 0.04~1.34$m$(which can be converted into 2.7~40.2$m$and 1.2~49.8$m$in the full-scale net) respectively, and the depth of model net A was slightly deeper than the depth of the model net B. 2. Working depth ($D$,which is appendixed m for the model net, f for the full-scale net, A and B for the types of the model nets) can be expressed as the function of towing velocity$V_t$, as in the model net($V_t$=$m$/$sec$) $D_{mA}$=(-1.99+0.65$L_w$) $e^{-1.72V_t}$ $D_{mA]$=(-1.91+1.04 $L_w$) $e^{2.88V_t}$ in the full-scale net($V_t$=$k$'$t$ $D_{fA}$=(-29.32+0.65$L_w$)$e^{0.40 V_t}$ $D_{fB}$=(-57.60+1.04$L_w$)$e^{-0.67 V_t}$ 3. Working depth 9$D$ appendixes are as same as the former) can be expressed as the function of warp length$L_w$) in the model net, and can be converted into full-scale net as in the model net ($V_t$=$m$/$sec$) $D_{mA}$=-0.99 $e^{-1.42V_t}$+0.67$e^{-1359V_t}$$L_w$ $D_{mB}$=-.258$e^{-3.77V_t}$+1.16$e^{-3.15V_t$ $L^w$, in the full-scale net($V_t$=k't) $D_{fA}$=-29.28$e^{-0.32V_t}$+0.67$e^{-0.37V_t$$L_w$ $D_{fB}$=-69.10$e^{-0.81V_t}$+1.16$e^{-0.72V_t}$$L_w$. 4. Working depth was gradually shallowed according to the increase of the distance between paired boats.

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A Study on the Physique and Bodily Strength of the Enlisted Men of Marine Corps in Korea (일부 사형의 체격 및 체력에 관한 연구)

  • Shin, Young-Soo
    • Journal of Preventive Medicine and Public Health
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    • v.6 no.1
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    • pp.87-99
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    • 1973
  • The purpose of this project is offering fundamental and proper informations for the better health control and personnel management of the enlisted men of Marine corps in Korea. Survey has been done under 1,001 marine enlisted men for the purpose of understanding their condition of physique, vital capacity, and bodily strength. 1. Under the subject of physique, 7 items, body weight, chest-girth, relative body weight, relative chest-girth, Vervaeck index, and Roehrer index are listed, and under the subject of vital capacity, BTPS vital capacity and percent predicted vital capacity are listed, and under the subject of bodily strength, 7 items, grasping power, chinning-up, throwing a hanp-grenade, forward jumping, sitting-up, 100 meter sprinting, are listed. The total items are 16 and mean score of each one is as follow. 1)Physique : a. Height : $$168{\pm}0.15cm$$ b. Body weight : $$62.7{\pm}0.17kg$$ c. Chest-girth : $$91.4{\pm}0.16cm$$ d. Relative body weight : $$37.2{\pm}0.09$$ e. Relative chest-girth : $$54.3{\pm}0.10$$ f. Vervaeck index : $$91.6{\pm}0.15$$ g. Roehrer index : $$1.31{\pm}0.003$$ 2) Vital capacity : a. BTPS vital capacity : $$4470{\pm}20cc$$ b. % Predicted vital capacity : $$150{\pm}5.1%$$ 3) Bodily strength : a. Grasping power : $$41.4{\pm}0.26kg$$ b. Chinning-up : $$5.7{\pm}0.10$$ c. Throwing a hand-grenade : $$39.7{\pm}0.20m$$ d. Forward jumping : $$214{\pm}0.58cm$$ e. Sitting-up : $$19.1{\pm}0.25$$ f. Pushing-up : $$22.1{\pm}0.18$$ g. 100 meter sprinting : $$16.1{\pm}0.04sec$$. 2. Comparative analysis has been done about the conditional classes of marine enlisted men with the results of above mentioned 16 items. 7 classes according to the branches, 3 according to the ranks, 9 according to tile length of service are adopted respectively.

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Antioxidative and antimelanogenic effects of ethyl acetate fractions of Korean domestic honeys from different floral sources (화종에 따른 국내산 벌꿀 에틸 아세테이트 분획물의 산화방지능 및 멜라닌 생성 억제 효과)

  • Jeong, Ha-Ram;Baek, Youngsu;Kim, Dae-Ok;Lee, Hyungjae
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.660-664
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    • 2018
  • Honey is known to have a variety of health-promoting effects. Ethyl acetate fractions (EAFs) of four Korean domestic honeys from basswood, Korean raisin, chestnut, and acacia as well as a New Zealand manuka honey were analyzed comparatively to evaluate their antioxidative and antimelanogenic effects. The EAFs of basswood, Korean raisin, and chestnut honey had higher antioxidant capacities and tyrosinase inhibition activities than those of manuka honey. Pretreatment of B16F1 melanoma cells with EAFs at $100{\mu}g/mL$ resulted in relative retention of melanin contents as follows: acacia honey (141.0%)>basswood honey (134.3%)>manuka honey (131.5%)>Korean raisin honey (107.3%)>chestnut honey (88.0%). Intracellular tyrosinase activities of B16F1 melanoma cells were significantly (p<0.05) lowered by EAFs of Korean raisin and chestnut honey than by EAF of manuka honey. Consequently, these results suggest that Korean domestic honeys from different floral sources serve as good sources of antioxidative and antimelanogenic agents.

Inhibition of Aqueous Extract from Amomum xanthioides on ${\alpha}$-melnocyte Stimulating Hormone Induced Melanogenesis in B16F10 Cell (사인의 열수 추출물이 B16F10 흑색종세포의 멜라닌형성에 미치는 영향)

  • Lee, Soo-Jin;Ye, Jeong-Sook;Choi, Yung-Hyun;Lee, Yong-Tae;Chung, Kyung-Tae;Jeong, Seong-Yun;Choi, Byung-Tae
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.1
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    • pp.50-53
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    • 2007
  • To develope skin-whitening or therapeutic agents against hyperpigmentation, aqueous extract from Amomum xanthioides (AEAX) was evaluated for melanogenesis inhibitory activity in B16F10 melanoma cell. The treatment with AEAX at the 0.5 and 1.0 mg/ml level significantly inhibits the biosynthesis of melanin compared with untreated control. The AEAX-treated cells at the 1.0 mg/ml level were more efficient than commercial arbutin at 0.1 mg/ml. The tyrosinase activity also significantly decreased in AEAX-treated cells at the 0.5 and 1.0 mg/ml level. The Western analyses confirmed the significantly decreased expression of tyrosinase and tyrosinase-related protein-1 by AEAX treatment. These results indicate that AEAX may contribute to the inhibition of melanin biosynthesis through regulating tyrosinase activity and expression and serve as a new candidate in the design of new skin-whitening or therapeutic agents.