• BMB Reports
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• 제48권7호
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• pp.401-406
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• 2015

Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed at the promoter area of ANGPT1. Using MTT and wound healing assays, depletion of KDM3B was found to increase cell proliferation and cell motility, indicating that KDM3B has a role in angiogenesis. [BMB Reports 2015; 48(7): 401-406]

• Mycobiology
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• 제51권2호
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• pp.72-78
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• 2023

In this study, a fungal strain KNUF-22-025 belonging to the genus Botryotrichum was isolated from the soil in Korea. The cultural and morphological characteristics of this strain differed from those of closely related species. On malt extract agar, strain KNUF-22-025 showed slower growth than most of the related species, except B. domesticum. The conidia size (9.6-21.1×9.9-18.4 ㎛) of strain KNUF-22-025 was larger than those of B. piluliferum, B. domesticum, and B. peruvianum but smaller than those of B. atrogriseum and B. iranicum. Conidiophores in strain KNUF-22-025 (137 ㎛) were longer than those in other closely related species but shorter than those in B. atrogriseum. Multi-locus analysis of molecular markers, such as ITS, 28S ribosomal DNA, RBP2, and TUB2 revealed that strain KNUF-22-025 was distinct from other Botryotrichum species. Thus, this strain is proposed as a novel species based on morphological characteristics along with molecular phylogeny and named Botryotrichum luteum sp. nov.

• 디지털융복합연구
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• 제15권10호
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• pp.19-29
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• 2017

본 연구는 베이비부머세대의 유형별 삶의 만족에 영향을 미치는 다차원적 요인을 규명하자고 하였다. 분석대상은 한국복지패널(10차년도) 응답자 중 1955년부터 1963년에 태어난 베이비부머세대 가구주 1,895명이었다. 베이비부머세대를 유형화하는데 활용한 기준은 성(gender), 소득지위, 종사상 지위로 남성과 여성 각각 4가지 유형으로 분류하였다. 남성 베이비부머세대의 삶의 만족에 대해 남성IV유형(B=.074, t=1.992, p<.05), 건강상태(B=.233, t=7.268, p<.001), 가구소득(B=.174, t=4.476, p<.001), 우울(B=-.182, t=-5.242, p<.001), 자아존중감(B=.255, t=7.027, p<.001), 신뢰(B=.064, t=2.036, p<.05) 등이 유의미한 영향을 미치는 것으로 분석되었다. 여성베이비부머세대의 삶의 만족에 대해 여성IV유형(B=.100, t=2.598, p<.05), 건강상태(B=.239, t=6.790, p<.001), 우울(B=-.181, t=-5.142, p<.001), 자아존중감(B=.320, t=8.600, p<.001) 등이 유의미한 영향을 미치는 것으로 분석되었다.

• 한국공작기계학회논문집
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• 제15권4호
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• pp.63-68
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• 2006

This research presents the reliability analysis of the pneumatic actuator within the tape feeder that is used to transfer the correct force to linked parts during l.0E+7 cycles. First, the degradation analysis for thrust and air leakage is executed to obtain the failure data of a product based on its performance over time. Second, once the parameters has been calculated using the weibull 2-parameter distribution and MLE(Maximum Likelihood Estimation), information related to life such as reliability, failure rate, probability density function is estimated. Finally, MTTF(Mean Time To Failure) and $B_{10}$ life of actuators are calculated. MTTF means the mean life at the confidence level and $B_{10}$ life refers to the time by which 10% of the product would fail. In this study, failure causes and solutions are examined using the reliability analysis.

• BMB Reports
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• 제38권3호
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• pp.290-293
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• 2005

In order to investigate the epitope of basic fibroblast growth factor (bFGF) and its immunogenicity, the epitopes of bFGF were screened from the phage display library with monoclonal antibody GF22, which can neutralize the bio-activity of bFGF. By three rounds of screening, the positive phage clones with bFGF epitopes were selected, which can effectively block the bFGF to bind with GF22. Sequence analysis showed that the epitopes shared a highly conservative sequence (Leu-Pro-Pro/Leu-Gly-His-Phe/Ile-Lys). The sequence of PPGHFK was located at 22-27 of the bFGF. The specific immuno-response of mouse could be highly induced by phage clones with the epitopes. And the anti-bFGF activity induced by LPGHFK was 3 times higher than the original sequence, which showed that the mimetic peptide LPLGHIK might be used as a tumor vaccine in the prevention and treatment of tumor.

• 생명과학회지
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• 제30권12호
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• pp.1033-1041
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• 2020

최근 모든 연령대의 사람들은 미용적인 이유로 더 밝은 피부를 원하고 있으며, 천연 제품은 화학적으로 합성된 화합물보다 더 많은 관심을 받고 있다. 조구등은 아시아에서 전통 한약재로 널리 사용되어 왔다. 새로운 피부 미백제를 찾기 위해 본 연구에서는 조구등의 항산화 활성과 잠재적인 tyrosinase 억제 작용을 확인하였다. 조구등을 70% 에탄올로 추출하여 항산화 활성을 분석하고 tyrosinase 활성과 melanin 합성에 미치는 영향을 평가했다. 총 mRNA 발현은 RT-PCR을 사용하였다. 그 결과 조구등 추출물은 B16F10 세포에서 뛰어난 항산화 능력과 상당한 수준의 폴리페놀 및 플라보노이드 화합물을 함유하였다. 또한, 세포 내 tyrosinase 활성을 억제하고 처리된 세포에서 melanin의 양을 감소시켰다. Tyrosinase의 mRNA 발현은 1 mg/ml 농도에서 현저히 감소하였다. 이와 같은 결과는 조구등이 미백 효과가 있는 화장품의 천연 소재로 사용될 수 있는 높은 잠재력을 가지고 있음을 시사한다.

• 한국어병학회지
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• 제26권3호
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• pp.185-191
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• 2013

본 연구는 E. tarda에 대한 특이박테리오phage와 생균제의 일종인 B. subtilis KM-1의 혼합제가 E. tarda에 대한 항균활성에 미치는 효과를 알아보고자 수행되었다. 가장 효과적인 혼합제의 항균활성을 보인 phage의 적정 수는 B. subtilis 가 $2{\times}10^5$ CFU/ml의 농도로 존재할 때 배양 36시간째 $2{\times}10^5$ PFU/ml로 나타났다. B. subtilis의 경우 $2{\times}10^5$ PFU/ml의 phage가 존재할 때 첨가량에 비례하는 항균활성을 보여주었다. phage와 B. subtilis의 혼합투여는 phage나 B. subtilis 각각을 투여한 그룹보다 훨씬 높은 항균활성을 나타냈다. 본 연구의 결과는 수산양식에서 대두되고 있는 양식어류의 E. tarda 감염성 질병 치료를 위한 항생제 대체제로서의 사료첨가제 개발 가능성을 제시하고 있다.

• 한국물환경학회지
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• 제34권1호
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• pp.46-56
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• 2018

A mcyB-specific Ultra-Rapid quantitative PCR was developed for the quantitative detection of Microcystis aeruginosa, which is often a dominant species in green tide. McyB-specific UR-qPCR was optimized under extremely short times of each step in thermal cycles, based on the specific primers deduced from the mcyB in microcystin synthetase of M. aeruginosa. The M. aeruginosa strain KG07 was used as a standard for quantification, after the microscopic counting and calculation by mcyB-specific UR-qPCR. The water samples from the river water with the Microcystis outbreak were also measured by using both methods. The $1.0{\times}10^8$ molecules of mcyB-specific DNA was recognized inner 4 minutes after beginning of UR-qPCR, while $1.0{\times}10^4$ molecules of mcyB-specific templates was detected inner 7 minutes with quantitative manner. From the range of $1.0{\times}10^2$ to $1.0{\times}10^8$ initial molecules, quantification was well established based on $C_T$ using mcyB-specific UR-qPCR (Regression coefficiency, $R^2=0.9977$). Between the numbers of M. aeruginosa cell counting under microscope and calculated numbers using mcyB-specific UR-qPCR, some differences were often found. The reasons for these differences were discussed; therefore, easy compensation method was proposed that was dependent on the numbers of the cell counting. Additionally, to easily extract the genomic DNA (gDNA) from the samples, a freeze-fracturing of water-sample using liquid nitrogen was tested, by excluding the conventional gDNA extraction method. It was also verified that there were no significant differences using the UR-qPCR with both gDNAs. In conclusion, the mcyB-specific UR-qPCR that we proposed would be expected to be a useful tool for rapid quantification and easy monitoring of M. aeruginosa in environmental water.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.806-811
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• 2007

Bacillus anthracis is a soil pathogen capable of causing anthrax that is closely related to several environmental species, including B. cereus, B. mycoides, and B. thuringiensis. DNA homology studies showed that B. anthracis, B. cereus, B. mycoides, and B. thuringiensis are closely related, with a high sequence homology. To establish a method to specifically detect B. anthracis in situations such as environmental contamination, we initially performed RAPD-PCR with a 10-mer random primer and confirmed the presence of specific PCR bands only in B. anthracis species. One region specific for B. anthracis was cloned and sequenced, and an internal primer set was designed to amplify a 241-bp DNA fragment within the sequenced region. The PCR system involving these specific primer sets has practical applications. Using lyses methods to prepare the samples for PCR, it was possible to quickly amplify the 241-bp DNA segment from samples containing only a few bacteria. Thus, the PCR detection method developed in this study is expected to facilitate the monitoring of environmental B. anthracis contamination.

• 산업융합연구
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• 제21권10호
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• pp.121-129
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• 2023

본 연구는 간호대학생을 대상으로 대인관계능력이 대학생활적응에 미치는 영향에서 정서지능과 자기효능의 병렬다중매개효과를 확인하고자 실시하였다. 연구대상자는 일개 대학 간호대학생 130명으로 선정하였다. 자료는 2023년 7월 24일 부터 8월 1일까지 수집되었으며, SPSS/WIN 27.0 프로그램을 활용하여, Descriptive statistics, Pearson's correlation, PROCESS macro model 4로 분석하였다. 연구 결과, 대인관계능력은 정서지능, 대학생활적응, 자기효능과 양의 상관관계가 있었다. 대인관계능력은 대학생활적응에 총효과(B=0.48, p<.001)가 유의하였고, 정서지능(B=0.20, 95% bootstrap CI=0.06~0.36)과 자기효능(B=0.17, 95% bootstrap CI=0.05~0.31)의 매개효과는 유의하였다. 그러므로 간호대학생의 대학생활적응 증진을 돕는 프로그램 개발에 정서지능과 자기효능을 함께 증진할 수 있는 다양한 훈련 프로그램의 개발 및 연구가 필요할 것이다.