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http://dx.doi.org/10.5352/JLS.2020.30.12.1033

The Effect of Inhibition of Uncaria rhynchophylla as an Inhibitor of Melanogenesis and an Antioxidant in B16F10 Melanoma Cells  

Dong, Yuanyuan (Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University)
Woo, Young Min (Department of Natural Science Institute, Silla University)
Cha, Ji Hyun (Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University)
Cha, Jae Young (Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University)
Lee, Nai Wei (Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University)
Back, Min Woo (Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University)
Park, Joon-sung (Division of Kinesiology, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University)
Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University)
Kim, Andre (Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University)
Publication Information
Journal of Life Science / v.30, no.12, 2020 , pp. 1033-1041 More about this Journal
Abstract
Many people of all ages wish to have lighter skin for cosmetic reasons, and natural products attract more attention than chemically synthesized compounds. Uncaria rhynchophylla is widely used in Asia as a traditional herbal medicine. In order to find novel skin whitening agents, the present study evaluated the antioxidant activity and potential tyrosinase-inhibiting properties of U. rhynchophylla. Specifically, this study analyzed the antioxidant capacity of a 70% ethanolic extract of U. rhynchophylla as well as its effects on tyrosinase activity and melanin synthesis. Total mRNA levels were examined using reverse transcription polymerase chain reaction. The results revealed that U. rhynchophylla extracts exhibit great antioxidant capacity and significant levels of polyphenol and flavonoid compounds. U. rhynchophylla extracts can also powerfully inhibit tyrosinase activity. This same capacity was observed in melanoma B16F10 cells; that is, U. rhynchophylla extracts suppressed intracellular tyrosinase activity and reduced the amount of melanin in treated cells. In addition, a 1 mg/ml concentration of U. rhynchophylla extract significantly reduced the mRNA expression levels of tyrosinase. U. rhynchophylla extracts decrease tyrosinase and inhibit melanogenesis in B16F10 cells. This finding suggests that U. rhynchophylla has great potential as a natural whitening agent in skincare products.
Keywords
Antioxidant; B16F10 melanoma cells; kojic acid; tyrosinase; Uncaria rhynchophylla;
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