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http://dx.doi.org/10.7847/jfp.2013.26.3.185

Mixture of Edwardsiella tarda specific Bacteriophage and Bacillus subtilis KM-1enhanced bactericidal activity against Edwardsiella tarda  

Baek, Min Suk (Department of Aquatic Life Medicine, Kunsan National University)
Hwang, Yo Sep (Department of Aquatic Life Medicine, Kunsan National University)
Choi, Sanghoon (Department of Aquatic Life Medicine, Kunsan National University)
Publication Information
Journal of fish pathology / v.26, no.3, 2013 , pp. 185-191 More about this Journal
Abstract
The present study was performed to investigate an antibacterial activity of specific bacteriophage (phage) and Bacillus subtilis KM-1 (B. subtilis) mixture against Edwardsiella tarda (E. tarda). An appropriate number of phage showing the most effective antibacterial activity was $2{\times}10^5$ PFU/ml with $1{\times}10^7$ CFU/ml of B. subtilis 36 h post incubation. On the other hand, B. subtilis showed a dose dependant manner in inducing antibacterial activity in the presence of phage ($2{\times}10^5$ PFU/ml). The phage and B. subtilis mixture showed higher antibacterial activity against E. tarda than phage or B. subtilis only. These results suggest that the phage and B. subtilis mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.
Keywords
Phage; B. subtilis; E. tarda; antibacterial activity;
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