Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Mixture of Edwardsiella tarda specific Bacteriophage and Bacillus subtilis KM-1enhanced bactericidal activity against Edwardsiella tarda

Edwardsiella tarda의 특이 Bacteriophage와 Bacillus subtilis KM-1혼합액이 Edwardsiella tarda 에 미치는 항균효과

  • Baek, Min Suk (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Hwang, Yo Sep (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sanghoon (Department of Aquatic Life Medicine, Kunsan National University)
  • 백민석 (군산대학교 해양과학대학 수산생명의학과) ;
  • 황요셉 (군산대학교 해양과학대학 수산생명의학과) ;
  • 최상훈 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2013.10.10
  • Accepted : 2013.11.19
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was performed to investigate an antibacterial activity of specific bacteriophage (phage) and Bacillus subtilis KM-1 (B. subtilis) mixture against Edwardsiella tarda (E. tarda). An appropriate number of phage showing the most effective antibacterial activity was $2{\times}10^5$ PFU/ml with $1{\times}10^7$ CFU/ml of B. subtilis 36 h post incubation. On the other hand, B. subtilis showed a dose dependant manner in inducing antibacterial activity in the presence of phage ($2{\times}10^5$ PFU/ml). The phage and B. subtilis mixture showed higher antibacterial activity against E. tarda than phage or B. subtilis only. These results suggest that the phage and B. subtilis mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.

본 연구는 E. tarda에 대한 특이박테리오phage와 생균제의 일종인 B. subtilis KM-1의 혼합제가 E. tarda에 대한 항균활성에 미치는 효과를 알아보고자 수행되었다. 가장 효과적인 혼합제의 항균활성을 보인 phage의 적정 수는 B. subtilis 가 $2{\times}10^5$ CFU/ml의 농도로 존재할 때 배양 36시간째 $2{\times}10^5$ PFU/ml로 나타났다. B. subtilis의 경우 $2{\times}10^5$ PFU/ml의 phage가 존재할 때 첨가량에 비례하는 항균활성을 보여주었다. phage와 B. subtilis의 혼합투여는 phage나 B. subtilis 각각을 투여한 그룹보다 훨씬 높은 항균활성을 나타냈다. 본 연구의 결과는 수산양식에서 대두되고 있는 양식어류의 E. tarda 감염성 질병 치료를 위한 항생제 대체제로서의 사료첨가제 개발 가능성을 제시하고 있다.

Keywords

References

  1. Ackermann, H.W. and Nguyen, T.M. 1983. Sewage coliphages studied by electron microscopy. Appl. Environ. Microbiol. 45(3): 1049-1059.
  2. Aly, S. M., Abdel-Galil Ahmed, Y., Abdel-Aziz Ghareeb, A. and Mohamed, M. F. 2008. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish Shellfish Immunol. 25: 128-136. https://doi.org/10.1016/j.fsi.2008.03.013
  3. Bang, J. D., Chun, S. K., Park, S. I. and Choi, Y. J. 1992. Studies on the biochemical and serological characteristics of Edwardsiella tarda isolated from cultured flounder, Paralichthys olivaceus. Fish Pathol. 5(1): 29-35.
  4. Barrow, P., Lovell, M. and Berchieri, A. Jr. 1998. Use of lytic bacteriophage for control of experimental Escherichia coli septicemia and meningitis in chickens and calves. Clin. Diagn. Lab. Immunol. 5: 294-298.
  5. Bizani, D. and Brandelli, A. 2002. Characterization of a bacteriocin produced by a newly isolated Bacillus sp. strain 8A. J. Appl. Microbiol. 93: 512-519. https://doi.org/10.1046/j.1365-2672.2002.01720.x
  6. Bradley, D. E. 1967. Ultrastructure of bacteriophages and bacteriocins. Bacteriol. Rev. 31(4): 230-314.
  7. Burr, G., Gatlin, D. and Ricke, S. 2005. Microbial ecology of the gastrointestinal tract of fish and the potential application of prebiotics and probiotics in finfish aquaculture. J. World Aquacul. Soc. 36: 425-436. https://doi.org/10.1111/j.1749-7345.2005.tb00390.x
  8. Carlton, R. M. 1999. Phage therapy: past history and future prospects. Immunol. Ther. Exp. 47: 267-274.
  9. Chinabut, S. and Puttinaowarat, S. 2005. The choice of disease control strategies to secure international market access for aquaculture products. Dev. Biol. 121: 255-261.
  10. Fuller, R. 1989. probiotics in man and animals. J. Appl. Bacteriol. 66: 365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  11. Kanai, K., Tawaki, S. and Uchida, Y. 1988. An ecological study of Edwardsiella tarda in flounder farm. Fish Pathol. 22: 41-47.
  12. Kumar, R., Mukherjee, S. C., Ranjan, R. and Nayak, S. K. 2008. Enhanced innate immune parameters in Labeo rohita (Ham.) following oral administration of Bacillus subtilis. Fish Shellfish Immunol. 24: 168-172. https://doi.org/10.1016/j.fsi.2007.10.008
  13. Kusuda, R. and Kawai, K. 1988. Bacterial diseases of cultured marine fish in Japan. Fish Pathol. 33: 221-227.
  14. Lee, C. H., Heo, Y. J., Baek, M. S., Lee, J. U., Kang, J. Y., Han, M. J., Kyoung, S. B. and Choi, S. H. 2011. Characterization of Edwardsiella tarda specific phage isolated from fish farms on west coast of Korea. J. Fish Pathol. 24(2): 85-93. https://doi.org/10.7847/jfp.2011.24.2.085
  15. Merril, C. R., Biswas, B., Carlton, R., Jensen, N. C., Creed, G. J., Zullo, S. and Adhya, S. 1996. Long-circulating bacteriophage as antibacterial agents. Proc. Natl. Acad. Sci. USA. 93: 3188-3192. https://doi.org/10.1073/pnas.93.8.3188
  16. Nayak, S. K. 2010. Probiotics and immunity: a fish perspective. Fish Shellfish Immunol. 29: 2-14. https://doi.org/10.1016/j.fsi.2010.02.017
  17. Nayak, S. K., Swain, P. and Mukherjee, S. C. 2007. Effect of dietary supplementation of probiotic and vitamin C on the immune response of Indian major carp. Fish Shellfish Immunol. 23: 892-896. https://doi.org/10.1016/j.fsi.2007.02.008
  18. Paek, N. S., Lim, Y. B. and Kim, Y. M. 2001. Antibacterial Activity and Growth Promotion in Aquacultured Fish by Probiotics. Kor. J. Appl, Microbiol. Biotechnol. 29(1): 56-61.
  19. Park, S. C., Ichiro, S., Minoru, F., Koh-Ichiro, M. and Toshihiro, N. 2000. Isolation of Bacteriophage Specific to a Fish Pathogen, Pseudomonas plecoglossicida, as a Candidate for Disease Control. Applied and Environmental Microbiology. 1416-1422.
  20. Rodgers, C. J., Pringle, J. H., McCarthy D. H. and Austin, B. 1981. Quantitative and qualitative studies of Aeromonas salmonicida bacteriophage. J. Gen. Microbiol. 125: 335-345.
  21. Salminen, S., Ouwehand, A., Benno, Y. and Lee, Y. K. 1999. Probiotics: How should they be defined Trends. Food Sci. Technol. 10: 107-110. https://doi.org/10.1016/S0924-2244(99)00027-8
  22. Shahani, K. M. and Ayebo, A. D. 1980. Role of dietary lactobacilli in gastrointestinal microccology. Am. J. Clin. Nutr. 33: 2448-2457.
  23. Smith, H. W. and Huggins, M. B. 1980. The association of the O18, K1 and H7 antigens and the Co1V plasmid of a strain of Escherichia coli with its virulence and immunogenecity. J. Gen. Microbiol. 128: 387-400.
  24. Smith, H. W. and Huggins, M. B. 1982. Successful treatment of experimental Escherichia coli infections in mice using phage: its general superiority over antibiotics. J. Gen. Microbiol. 128: 307-318.
  25. Soothill, J. S. 1992. Treatment of experimental infections of mice with bacteriophages. J. Med. Microbiol. 37: 258-261. https://doi.org/10.1099/00222615-37-4-258
  26. Soothill, J. S. 1994. Bacteriophage prevents destruction of skin grafts by Pseudomonas aeruginosa. Burns. 20: 209-211. https://doi.org/10.1016/0305-4179(94)90184-8
  27. Stevenson, R. M. W. and Airdrie, D. W. 1984. Isolation of Yersinia ruckeri bacteriophages. Appl. Environ. Microbiol. 47: 1201-1205.
  28. Sulakvelidze, A., Alavidze, Z. and Morris, J. G., Jr. 2001. Bacteriophage therapy. Antimicrob. Agents Chemother. 45: 649-659. https://doi.org/10.1128/AAC.45.3.649-659.2001
  29. Tournut, J. 1989. Application of probiotics to animal husbundary. Rev. Sci. Tech. Off. Int. Epiz. 8: 551-566.
  30. Wang, Y., Tian, Z., Yao, j. and Li, W. 2008. Effect of probiotics, Enterococcus faecium, on tilapia (Oreochromis niloticus) growth performance and immune response. Aquaculture. 277: 203-207. https://doi.org/10.1016/j.aquaculture.2008.03.007
  31. Zheng, G., Yan, L. Z., Vederas, J. C. and Zuber, P., 1999. Genes of the sbo-alb locus of Bacillus subtilis are required for production of the antilisterial bacteriocin subtilosin. J. Bacteriol. 181: 7346-7355.

Cited by

  1. The Effects of a Dietary Edwardsiella tarda Specific Bacteriophage and Bacillus subtilis Mixture on Innate Immune Responses and Antibacterial Activity of Nile tilapia Oreochromis niloticus vol.47, pp.1, 2014, https://doi.org/10.5657/KFAS.2014.0023